FastQCFastQC Report
Fri 27 May 2016
SRR2139667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5705390
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105600.039.8102261
GTATCAA174750.034.088861
TCAACGC191600.030.3792464
ATCAACG192200.030.2185253
CAACGCA193400.030.03055
TATCAAC197000.029.8067382
AACGCAG200200.029.0317366
ACGCAGA230700.025.1382277
CGCAGAG234150.024.7496648
GCAGAGT271050.021.4431069
GTACATG193350.020.6864171
GAGTACT154500.020.57209412-13
TACATGG189400.020.3090722
ACATGGG188800.019.7422263
CTTATAC40950.019.1190171
GTACTTT165900.019.05620414-15
GTGGTAT63950.018.9629521
CAGAGTA263400.018.79476710-11
TGGTATC60700.018.786582
CGTTCGG1750.00692302918.55278294
Produced by FastQC (version 0.11.5)