##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139667_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5705390
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.270596751492885	25.0	18.0	33.0	18.0	33.0
2	27.48249024168374	29.0	25.0	31.0	18.0	33.0
3	26.77967062724897	29.0	25.0	31.0	18.0	33.0
4	30.950172380853893	31.0	30.0	33.0	27.0	33.0
5	31.713724390444824	33.0	31.0	33.0	29.0	33.0
6	36.07008004711334	38.0	36.0	38.0	33.0	38.0
7	36.37619251269414	38.0	37.0	38.0	34.0	38.0
8	36.50823256604719	38.0	37.0	38.0	34.0	38.0
9	37.188449869334086	38.0	38.0	38.0	36.0	38.0
10-11	37.23829448994722	38.0	38.0	38.0	36.0	38.0
12-13	37.34241182460796	38.0	38.0	38.0	36.5	38.0
14-15	37.32923183165393	38.0	38.0	38.0	36.0	38.0
16-17	37.43686145907641	38.0	38.0	38.0	37.0	38.0
18-19	37.46293277059061	38.0	38.0	38.0	37.0	38.0
20-21	37.49335553429883	38.0	38.0	38.0	37.0	38.0
22-23	37.517203856641686	38.0	38.0	38.0	37.0	38.0
24-25	37.527072266891324	38.0	38.0	38.0	37.0	38.0
26-27	37.497221752333104	38.0	38.0	38.0	37.0	38.0
28-29	37.47393422721868	38.0	38.0	38.0	37.0	38.0
30-31	37.48566478886699	38.0	38.0	38.0	37.0	38.0
32-33	37.47337129121587	38.0	38.0	38.0	37.0	38.0
34-35	37.43903183598409	38.0	38.0	38.0	37.0	38.0
36-37	37.42091615600431	38.0	38.0	38.0	37.0	38.0
38-39	37.407747328006266	38.0	38.0	38.0	37.0	38.0
40-41	37.40463828640379	38.0	38.0	38.0	37.0	38.0
42-43	37.37373997713611	38.0	38.0	38.0	37.0	38.0
44-45	37.33730957157153	38.0	38.0	38.0	37.0	38.0
46-47	37.29788491466483	38.0	38.0	38.0	37.0	38.0
48-49	37.238624314994524	38.0	38.0	38.0	37.0	38.0
50-51	37.161666719292576	38.0	38.0	38.0	37.0	38.0
52-53	37.18626595980709	38.0	38.0	38.0	37.0	38.0
54-55	37.17160486061391	38.0	38.0	38.0	37.0	38.0
56-57	37.12152637481283	38.0	38.0	38.0	36.0	38.0
58-59	37.108857873763334	38.0	38.0	38.0	36.0	38.0
60-61	37.12646484710859	38.0	38.0	38.0	36.0	38.0
62-63	37.129585319819434	38.0	38.0	38.0	36.0	38.0
64-65	37.07477619412588	38.0	38.0	38.0	36.0	38.0
66-67	37.114289482824944	38.0	38.0	38.0	36.0	38.0
68-69	37.128369228501406	38.0	38.0	38.0	36.0	38.0
70-71	37.1145018391023	38.0	38.0	38.0	36.0	38.0
72-73	37.10898476191119	38.0	38.0	38.0	36.0	38.0
74-75	37.07159395826066	38.0	38.0	38.0	36.0	38.0
76-77	37.08067277007305	38.0	38.0	38.0	36.0	38.0
78-79	37.082212648044305	38.0	38.0	38.0	36.0	38.0
80-81	37.078994668688495	38.0	38.0	38.0	36.0	38.0
82-83	37.035561516087206	38.0	38.0	38.0	36.0	38.0
84-85	37.02545304082034	38.0	38.0	38.0	36.0	38.0
86-87	36.99520674246813	38.0	38.0	38.0	36.0	38.0
88-89	37.00574435482469	38.0	38.0	38.0	36.0	38.0
90-91	36.99593582515808	38.0	38.0	38.0	36.0	38.0
92-93	36.9795916988747	38.0	38.0	38.0	36.0	38.0
94-95	36.96960471165473	38.0	38.0	38.0	35.5	38.0
96-97	36.954984839131654	38.0	38.0	38.0	35.0	38.0
98-99	36.988846216112336	38.0	38.0	38.0	35.0	38.0
100	35.56179545318166	38.0	36.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	15.0
21	77.0
22	308.0
23	827.0
24	2037.0
25	4203.0
26	7736.0
27	13613.0
28	22432.0
29	33010.0
30	43118.0
31	55650.0
32	72205.0
33	99901.0
34	160370.0
35	338056.0
36	1221694.0
37	3630135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.35799305568944	18.808162106359074	10.406247425679927	21.427597412271552
2	14.962293270279911	20.094106882913266	41.33197434277236	23.611625504034468
3	19.853516359302947	25.325067870543194	29.4729663243971	25.34844944575676
4	12.870347422593317	17.65351196220554	39.05038151669317	30.42575909850797
5	13.662624290364025	37.67558396533804	34.9707557239733	13.69103602032464
6	30.35200047674217	37.22557090751027	18.666489056839232	13.75593955890833
7	27.11222787498243	32.82363259621543	22.31229714300938	17.751842385792763
8	24.32254412055968	36.57034838985591	20.98990603622189	18.117201453362522
9	25.601825642068288	17.346351432592687	21.353194084891655	35.69862884044737
10-11	24.247693847396935	26.872185775205555	28.52231836912113	20.357802008276384
12-13	25.175579583516637	24.367221171558825	28.768418986256854	21.68878025866768
14-15	22.272395050995637	25.85270244453052	26.70642147162595	25.168481032847883
16-17	21.597007391256337	29.019260734147885	27.50835613341069	21.875375741185092
18-19	21.46910202457676	28.082891791796882	29.36906153654702	21.07894464707934
20-21	22.501263749631043	27.1105670502436	29.35742099724394	21.03074820288142
22-23	22.063034687694355	27.12046770996429	29.238332317802136	21.57816528453922
24-25	21.8580659027948	27.174015944326342	29.4634313786334	21.504486774245464
26-27	21.783207507437425	27.386047010731247	29.35039155737666	21.48035392445467
28-29	21.610003362802146	27.48864462233206	29.41887024934029	21.482481765525506
30-31	22.07939720633508	27.247202282866585	29.352043698220687	21.321356812577648
32-33	21.653470524058868	27.48765465611351	29.317865470882005	21.541009348945618
34-35	21.902197205747903	27.477015246517112	29.31160408523324	21.309183462501746
36-37	21.825909298822825	27.32257502373652	29.289749040993364	21.561766636447295
38-39	21.642875719223028	27.36690976029256	29.486918737044448	21.50329578343996
40-41	21.963618153081587	27.261821166561383	29.32027367084013	21.454287009516896
42-43	21.91567120722366	27.26343599317399	29.132857530933055	21.68803526866929
44-45	21.863727314456334	27.512645796547513	28.988462795603713	21.635164093392437
46-47	21.983406579941555	27.383239738233772	28.674875993917865	21.958477687906804
48-49	21.945589621880725	27.4363667980567	28.71578819744025	21.90225538262233
50-51	21.875485160049422	27.667299542032282	28.63330606856533	21.823909229352967
52-53	22.04789140919582	27.652887348662368	28.542390452947963	21.75683078919385
54-55	22.051705469149237	27.596245621574994	28.476068912783823	21.875979996491946
56-57	22.108775727742202	27.77968009639776	28.285684082074454	21.825860093785586
58-59	21.941130571483967	27.874743668675457	28.515336503483752	21.66878925635683
60-61	22.08165528952886	27.943999406926935	28.331795934936142	21.642549368608062
62-63	21.92389942476423	28.064001672527162	28.34155433222752	21.67054457048109
64-65	21.9065663822253	27.986921695454974	28.356438496573666	21.75007342574606
66-67	22.05470918083495	27.958931948124295	28.210444952917346	21.775913918123415
68-69	22.027514543184793	27.86435795187482	28.354808222016686	21.753319282923698
70-71	22.007238126726786	27.570752377127228	28.508964489735156	21.913045006410833
72-73	22.105648705255714	27.55830089962808	28.47985148459131	21.8561989105249
74-75	21.91988815388138	27.709502761498094	28.3806711913594	21.989937893261125
76-77	21.96649626571142	27.656160273921138	28.382171680356727	21.995171780010715
78-79	22.006863346193782	27.71623452218066	28.255773808055906	22.021128323569656
80-81	21.978093985062557	27.71652073255515	28.243095235435305	22.062290046946988
82-83	22.1164490018018	27.637285637419396	28.349287075731567	21.89697828504724
84-85	22.192505073761247	27.603497548929056	28.27683085960637	21.927166517703327
86-87	22.006531612399503	27.721731212757028	28.257842911588515	22.013894263254958
88-89	22.15940941540154	27.710831240795613	28.202321189565456	21.927438154237393
90-91	22.140427186188408	27.75203179548357	28.204337664994284	21.90320335333374
92-93	22.180578066296665	27.73311990004182	28.202085780273933	21.884216253387574
94-95	22.02470297263487	27.854110973553432	28.184218543248722	21.93696751056298
96-97	22.157071384870317	27.987909969630287	28.20432076067852	21.65069788482087
98-99	23.208642630947963	29.828442104907676	28.468563081224435	18.49435218291993
100	22.19475174234696	30.78459726710843	22.29346866347201	24.7271823270726
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	946.0
1	952.0
2	1245.5
3	1707.0
4	2202.5
5	3146.0
6	4116.0
7	5065.0
8	6735.0
9	7890.5
10	7637.5
11	7375.5
12	8068.0
13	10209.0
14	14073.0
15	18336.0
16	22391.5
17	25807.0
18	26580.5
19	24740.0
20	22157.5
21	19858.5
22	19071.5
23	21114.5
24	25771.5
25	32720.5
26	42758.5
27	54743.5
28	65497.0
29	77167.0
30	89876.5
31	101987.0
32	115912.5
33	130718.5
34	145747.5
35	161011.5
36	178404.0
37	192139.0
38	199242.5
39	206796.5
40	216077.5
41	223097.5
42	227486.5
43	236594.0
44	246527.5
45	252908.5
46	254868.0
47	253894.5
48	252909.5
49	248685.0
50	242013.5
51	231806.5
52	218494.5
53	203721.0
54	187757.0
55	171478.0
56	154953.5
57	138573.0
58	123175.5
59	107192.5
60	90034.5
61	72567.0
62	56300.5
63	43275.0
64	32618.5
65	24067.0
66	17579.0
67	13172.5
68	10078.0
69	7521.0
70	5476.5
71	3946.0
72	2810.5
73	1942.5
74	1314.5
75	862.5
76	564.0
77	340.0
78	225.5
79	163.0
80	105.5
81	69.5
82	51.0
83	34.5
84	20.5
85	12.0
86	10.5
87	12.5
88	10.0
89	6.5
90	4.5
91	3.0
92	2.5
93	1.5
94	0.5
95	1.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003067274980325622
3	7.5367328088001E-4
4	7.010914240744279E-5
5	0.0
6	0.0
7	2.8043656962977115E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.732890556814056E-4
24-25	3.3309274687037447E-4
26-27	6.136846952910834E-5
28-29	0.002447049660636312
30-31	0.0017209535311815268
32-33	0.004320166767235925
34-35	0.008955643184587895
36-37	0.0011782847611280136
38-39	0.0
40-41	1.3457262157694352E-4
42-43	6.050748075658915E-4
44-45	2.1845029359264352E-4
46-47	3.674116857123699E-5
48-49	0.0040968874963178996
50-51	0.0031591362547617203
52-53	4.5205780952939747E-4
54-55	0.002562637018032043
56-57	0.03958829702555589
58-59	0.06084391142354575
60-61	0.02898281706554236
62-63	0.010481669554119404
64-65	8.521673886638352E-4
66-67	2.5011174992986866E-4
68-69	0.0013039418769424565
70-71	0.010461401972934596
72-73	0.01603400323736127
74-75	0.010108819882818977
76-77	0.011931799350064158
78-79	0.002625452239462928
80-81	0.01372643494410935
82-83	0.043408448760015955
84-85	0.023706463348580144
86-87	0.0035538750856085335
88-89	6.713421920665256E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	72.0
20-21	391.0
22-23	699.0
24-25	714.0
26-27	1877.0
28-29	4490.0
30-31	11223.0
32-33	14705.0
34-35	19954.0
36-37	31903.0
38-39	37180.0
40-41	36219.0
42-43	40922.0
44-45	48832.0
46-47	50039.0
48-49	46021.0
50-51	41228.0
52-53	40189.0
54-55	43860.0
56-57	44931.0
58-59	44549.0
60-61	44810.0
62-63	43419.0
64-65	47155.0
66-67	50481.0
68-69	51468.0
70-71	53798.0
72-73	45351.0
74-75	45248.0
76-77	47506.0
78-79	48721.0
80-81	48413.0
82-83	45758.0
84-85	45454.0
86-87	47109.0
88-89	48890.0
90-91	49809.0
92-93	49141.0
94-95	55813.0
96-97	170382.0
98-99	373770.0
100-101	3732896.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.09830896671113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.33770456990365	82.2713333104617
2	5.6699868740931585	10.103724846902978
3	1.163443569167771	3.109825637731296
4	0.39442334770005294	1.405698131882554
5	0.17667022925457276	0.7870509335671806
6	0.09138823665726911	0.48855224073673686
7	0.05325675570918345	0.33215608123190393
8	0.03306041817006628	0.23565018821481892
9	0.018827193201062867	0.15097239691238362
>10	0.05889396562144018	0.8190948755986619
>50	0.0014502390714954834	0.08392430798688487
>100	8.12564876814838E-4	0.15931686087828847
>500	5.469106901037953E-5	0.02689830733819168
>1k	2.7345504420903237E-5	0.025801880556471098
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7527285601860697E-5
2	0.0	0.0	0.0	0.0	1.7527285601860697E-5
3	0.0	0.0	0.0	0.0	1.7527285601860697E-5
4	0.0	1.2269099921302488E-4	0.0	0.0	1.7527285601860697E-5
5	0.0	1.2269099921302488E-4	0.0	0.0	1.7527285601860697E-5
6	1.7527285601860697E-5	1.2269099921302488E-4	0.0	0.0	1.7527285601860697E-5
7	1.7527285601860697E-5	1.2269099921302488E-4	0.0	1.7527285601860697E-5	1.7527285601860697E-5
8	1.7527285601860697E-5	1.2269099921302488E-4	0.0	1.7527285601860697E-5	1.7527285601860697E-5
9	1.7527285601860697E-5	1.4021828481488558E-4	0.0	1.7527285601860697E-5	1.7527285601860697E-5
10-11	1.7527285601860697E-5	1.4021828481488558E-4	0.0	5.2581856805582094E-5	1.7527285601860697E-5
12-13	1.7527285601860697E-5	1.4021828481488558E-4	0.0	7.010914240744279E-5	1.7527285601860697E-5
14-15	1.7527285601860697E-5	1.9280014162046766E-4	0.0	1.0516371361116419E-4	1.7527285601860697E-5
16-17	3.5054571203721394E-5	1.9280014162046766E-4	0.0	1.4021828481488558E-4	1.7527285601860697E-5
18-19	3.5054571203721394E-5	2.01563784421398E-4	0.0	1.665092132176766E-4	1.7527285601860697E-5
20-21	3.5054571203721394E-5	2.1032742722232835E-4	0.0	1.9280014162046766E-4	1.7527285601860697E-5
22-23	3.5054571203721394E-5	2.1032742722232835E-4	0.0	2.3661835562511938E-4	3.5054571203721394E-5
24-25	3.5054571203721394E-5	2.190910700232587E-4	0.0	2.892002124307015E-4	5.258185680558209E-5
26-27	3.5054571203721394E-5	2.2785471282418904E-4	0.0	4.1189121164372637E-4	5.258185680558209E-5
28-29	3.5054571203721394E-5	2.2785471282418904E-4	0.0	9.377097796995473E-4	5.258185680558209E-5
30-31	3.5054571203721394E-5	2.541456412269801E-4	0.0	0.0022960744138437513	5.258185680558209E-5
32-33	3.5054571203721394E-5	2.6290928402791043E-4	0.0	0.004968985468127508	5.258185680558209E-5
34-35	5.258185680558209E-5	2.804365696297711E-4	0.0	0.00829916973248104	5.258185680558209E-5
36-37	5.258185680558209E-5	2.979638552316318E-4	0.0	0.013522300841835528	5.258185680558209E-5
38-39	5.258185680558209E-5	3.1549114083349254E-4	0.0	0.02133070657746447	5.258185680558209E-5
40-41	5.258185680558209E-5	3.5930935483814426E-4	0.0	0.02941078523992225	5.258185680558209E-5
42-43	5.258185680558209E-5	3.6807299763907465E-4	0.0	0.037166609118745614	6.134549960651244E-5
44-45	6.134549960651244E-5	4.2065485444465675E-4	0.0	0.04580756092046293	7.010914240744279E-5
46-47	7.010914240744279E-5	4.732367112502388E-4	0.0	0.05489545850502771	7.010914240744279E-5
48-49	7.010914240744279E-5	4.995276396530298E-4	0.0	0.06461433837125946	7.010914240744279E-5
50-51	7.010914240744279E-5	6.485095672688457E-4	0.0	0.07370223595582423	7.010914240744279E-5
52-53	7.010914240744279E-5	6.485095672688457E-4	0.0	0.08279889718318993	8.763642800930348E-5
54-55	7.010914240744279E-5	6.485095672688457E-4	0.0	0.09092279405965237	1.2269099921302488E-4
56-57	7.010914240744279E-5	6.485095672688457E-4	0.0	0.09760945351676223	1.4021828481488558E-4
58-59	7.010914240744279E-5	6.572732100697761E-4	0.0	0.1041558946890572	1.4898192761581594E-4
60-61	8.763642800930348E-5	6.660368528707065E-4	0.0	0.11067604493294937	1.5774557041674627E-4
62-63	1.0516371361116417E-4	6.748004956716369E-4	0.0	0.11682812217920246	1.5774557041674627E-4
64-65	1.0516371361116417E-4	7.186187096762885E-4	0.0	0.12211259878816347	1.5774557041674627E-4
66-67	1.0516371361116417E-4	7.361459952781493E-4	0.0	0.12722180254110588	1.5774557041674627E-4
68-69	1.0516371361116417E-4	7.361459952781493E-4	0.0	0.1325150427928678	1.5774557041674627E-4
70-71	1.0516371361116417E-4	7.624369236809403E-4	0.0	0.1375716646890046	1.5774557041674627E-4
72-73	1.0516371361116417E-4	7.887278520837314E-4	0.0	0.14174315866224746	1.7527285601860696E-4
74-75	1.0516371361116417E-4	7.887278520837314E-4	0.0	0.14483672457097585	1.7527285601860696E-4
76-77	1.0516371361116417E-4	8.413097088893135E-4	0.0	0.14789523590850057	1.7527285601860696E-4
78-79	1.0516371361116417E-4	8.763642800930348E-4	0.0	0.1502438921791499	1.7527285601860696E-4
80-81	1.2269099921302488E-4	8.763642800930348E-4	0.0	0.1509975654600299	1.7527285601860696E-4
82-83	1.2269099921302488E-4	8.763642800930348E-4	0.0	0.15147080217128012	1.9280014162046766E-4
84-85	1.2269099921302488E-4	8.763642800930348E-4	0.0	0.15172494781250712	1.9280014162046766E-4
86-87	1.2269099921302488E-4	8.938915656948955E-4	0.0	0.1518301115261183	1.9280014162046766E-4
88	1.2269099921302488E-4	9.464734225004776E-4	0.0	0.15183887516891922	2.1032742722232835E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10560	0.0	39.810226	1
GTATCAA	17475	0.0	34.08886	1
TCAACGC	19160	0.0	30.379246	4
ATCAACG	19220	0.0	30.218525	3
CAACGCA	19340	0.0	30.0305	5
TATCAAC	19700	0.0	29.806738	2
AACGCAG	20020	0.0	29.031736	6
ACGCAGA	23070	0.0	25.138227	7
CGCAGAG	23415	0.0	24.749664	8
GCAGAGT	27105	0.0	21.443106	9
GTACATG	19335	0.0	20.686417	1
GAGTACT	15450	0.0	20.572094	12-13
TACATGG	18940	0.0	20.309072	2
ACATGGG	18880	0.0	19.742226	3
CTTATAC	4095	0.0	19.119017	1
GTACTTT	16590	0.0	19.056204	14-15
GTGGTAT	6395	0.0	18.962952	1
CAGAGTA	26340	0.0	18.794767	10-11
TGGTATC	6070	0.0	18.78658	2
CGTTCGG	175	0.006923029	18.552782	94
>>END_MODULE