FastQCFastQC Report
Fri 27 May 2016
SRR2139666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21000161
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA327250.051.157121
GTATCAA611500.037.1155551
TCAACGC783150.028.5093364
ATCAACG786150.028.3899923
CAACGCA789100.028.250235
TATCAAC809950.027.7196042
AACGCAG815900.027.439056
ACGCAGA924350.024.094067
CGCAGAG936750.023.7882678
GCAGAGT1054450.020.7443079
GTACATG801000.019.968441
TACATGG794100.019.598842
ACATGGG791450.019.3081653
AGAGTAC988150.018.66550610-11
GAGTACT601250.018.3815912-13
CAGAGTA1047300.018.09551210-11
CTTATAC134350.017.7791841
CATGGGG469150.017.2984074
AGTACTT657600.016.84423412-13
GTACTTT665150.016.56902914-15
Produced by FastQC (version 0.11.5)