##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21000161
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.568051787793436	31.0	31.0	34.0	28.0	34.0
2	31.59427272962336	31.0	31.0	34.0	28.0	34.0
3	31.68059216307913	33.0	31.0	34.0	28.0	34.0
4	35.23196988823086	37.0	35.0	37.0	32.0	37.0
5	35.067223341763906	37.0	35.0	37.0	32.0	37.0
6	35.11470616820509	37.0	35.0	37.0	32.0	37.0
7	34.97694684340753	37.0	35.0	37.0	32.0	37.0
8	34.99784844506668	37.0	35.0	37.0	32.0	37.0
9	36.50828948406634	39.0	37.0	39.0	32.0	39.0
10-11	36.446997763493336	39.0	36.0	39.0	32.0	39.0
12-13	36.4317492613509	39.0	35.0	39.0	32.0	39.0
14-15	37.59714258857349	40.0	37.0	41.0	31.0	41.0
16-17	37.62376017021965	40.0	37.0	41.0	31.5	41.0
18-19	37.68962533192008	40.0	37.0	41.0	31.5	41.0
20-21	37.71649377605879	40.0	37.0	41.0	31.0	41.0
22-23	37.67962741033428	40.0	37.0	41.0	31.0	41.0
24-25	37.6738297362071	40.0	37.0	41.0	31.0	41.0
26-27	37.60049755470874	40.0	37.0	41.0	31.0	41.0
28-29	37.54167445344054	40.0	37.0	41.0	31.0	41.0
30-31	37.43856900070679	40.0	36.0	41.0	31.0	41.0
32-33	37.356935674561626	40.0	36.0	41.0	31.0	41.0
34-35	37.27346471095	40.0	36.0	41.0	30.0	41.0
36-37	37.24351198126705	40.0	36.0	41.0	30.0	41.0
38-39	37.18429980029538	40.0	36.0	41.0	30.0	41.0
40-41	37.170122866582886	40.0	36.0	41.0	30.0	41.0
42-43	37.14057725883063	40.0	36.0	41.0	30.0	41.0
44-45	37.0760539807133	40.0	35.0	41.0	30.0	41.0
46-47	37.00892610221092	39.5	35.0	41.0	30.0	41.0
48-49	36.953365271985774	39.0	35.0	41.0	30.0	41.0
50-51	36.88237299459822	39.0	35.0	41.0	30.0	41.0
52-53	37.106738489707105	39.0	35.5	41.0	30.0	41.0
54-55	37.10598469435365	39.0	35.0	41.0	30.0	41.0
56-57	37.03263348755449	39.0	35.0	41.0	30.0	41.0
58-59	36.90636910215824	39.0	35.0	41.0	30.0	41.0
60-61	36.79176996864001	39.0	35.0	41.0	30.0	41.0
62-63	36.638565406175076	39.0	35.0	41.0	30.0	41.0
64-65	36.450270641009425	38.0	35.0	40.5	30.0	41.0
66-67	36.236709526509735	38.0	34.5	40.0	29.5	41.0
68-69	35.988142463442486	37.0	34.0	40.0	29.0	41.0
70-71	35.746094559591754	37.0	34.0	39.5	29.0	41.0
72-73	35.47337643525815	36.0	34.0	39.0	29.0	41.0
74-75	35.2047226916405	36.0	34.0	39.0	29.0	41.0
76-77	34.909724897482505	35.5	34.0	38.5	29.0	40.0
78-79	34.63028595271969	35.0	34.0	37.0	29.0	39.0
80-81	34.365756659311984	35.0	34.0	37.0	29.0	39.0
82-83	34.094407743098145	35.0	33.0	36.5	29.0	39.0
84-85	33.8693524123036	35.0	33.0	36.0	29.0	37.0
86-87	33.64371954477364	35.0	33.0	36.0	29.0	37.0
88-89	33.498733926177636	35.0	33.0	35.0	29.0	37.0
90-91	33.39344644175948	35.0	33.0	35.0	29.0	36.0
92-93	33.320048007972304	35.0	33.0	35.0	29.0	36.0
94-95	33.37039675705297	35.0	33.0	35.0	29.0	36.0
96-97	33.552093733579426	35.0	33.5	35.0	30.0	36.0
98-99	33.649402821368994	35.0	34.0	35.0	30.0	36.0
100	32.85320122459728	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	7.0
14	13.0
15	19.0
16	70.0
17	309.0
18	2153.0
19	11048.0
20	33804.0
21	75932.0
22	125616.0
23	172772.0
24	216377.0
25	254244.0
26	290933.0
27	328037.0
28	369498.0
29	420142.0
30	484037.0
31	567332.0
32	681094.0
33	836722.0
34	1075824.0
35	1422471.0
36	2062655.0
37	3470305.0
38	4945622.0
39	2996249.0
40	156876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94788897094646	21.713814479803272	14.4616605558405	23.876635993409764
2	14.15116620031481	22.917698442267483	41.0854564276808	21.8456789297369
3	16.4707546765951	28.959054171060878	31.882922230929562	22.68726892141446
4	11.583469307789493	19.978518404016686	40.46577882681516	27.97223346137866
5	11.645080742500026	39.587142821892236	36.66493303477645	12.102843400831285
6	26.989017722915786	41.0143921217559	19.685752745688323	12.310837409639998
7	24.06007346604767	34.23180092392584	25.190432079716985	16.517693530309508
8	23.612554624817918	36.99085764952212	22.379520639693105	17.017067085966854
9	25.644953963390332	15.964332615588189	21.860387220472965	36.530326200548515
10-11	22.920383530460605	27.41375711269302	30.492662649253244	19.17319670759313
12-13	24.242951944207263	25.426874270868403	30.150851758080048	20.179322026844282
14-15	21.58854154823993	26.481447191602136	27.863489733727846	24.066521526430083
16-17	20.614687152873362	29.422648417403725	28.517818574128356	21.444845855594558
18-19	20.669311154706552	28.558323227853812	30.382309504528486	20.39005611291115
20-21	21.85384616796135	27.655034436421577	30.06263487817893	20.428484517438143
22-23	21.4631938127002	27.633423986278615	29.892101451233145	21.01128074978804
24-25	21.151784657783505	27.857761562584592	30.026446231947684	20.96400754768422
26-27	21.066241314994333	27.998725342235296	29.918133695846933	21.016899646923438
28-29	21.047176067439633	27.806014030142055	30.054259878896044	21.092550023522268
30-31	21.335997899572696	27.810008909126623	29.8618599876001	20.99213320370059
32-33	21.0504971800701	28.000509990146206	29.821905437654117	21.127087392129578
34-35	21.178520609232493	28.064576534173796	29.763378702250403	20.99352415434331
36-37	21.24329289392079	27.941154601534674	29.659886111877277	21.155666392667264
38-39	21.133727220687778	28.0716211439683	29.686673278047216	21.1079783572967
40-41	21.32159093252577	27.95260482269256	29.622840964252966	21.1029632805287
42-43	21.315018584421907	28.06222691856826	29.261964049938033	21.360790447071796
44-45	21.325946105589928	28.276246231007544	29.043427931116295	21.354379732286237
46-47	21.544159537592684	28.19787228402738	28.62197615408148	21.63599202429846
48-49	21.51912245909022	28.31272672780259	28.598745296780148	21.569405516327045
50-51	21.474429536658686	28.380543212397658	28.420657439242536	21.724369811701116
52-53	21.591475171085193	28.340560501408678	28.44162786460017	21.62633646290596
54-55	21.637696265187117	28.29586930476634	28.457092016017192	21.60934241402935
56-57	21.654181203068518	28.395074949779875	28.320333032996952	21.630410814154654
58-59	21.512788906603834	28.468206077903684	28.451074796333003	21.56793021915947
60-61	21.675698718058726	28.620576272931647	28.216368347891745	21.48735666111788
62-63	21.545232332973058	28.64603939030914	28.29544251802244	21.51328575869536
64-65	21.499159178828844	28.497159138889106	28.337873514649857	21.66580816763219
66-67	21.649943997403817	28.574868708672135	28.14691870820659	21.628268585717453
68-69	21.608355071959934	28.516708913566536	28.267791266837598	21.607144747635935
70-71	21.639375084068714	28.163955516410184	28.4094433969371	21.787226002584003
72-73	21.681816200207777	28.29753818750233	28.22524999811887	21.795395614171024
74-75	21.496810622189496	28.437319380484716	28.25439417858587	21.811475818739922
76-77	21.5810962405237	28.376681539247855	28.29066998989454	21.751552230333903
78-79	21.563067342787214	28.381680639039423	28.222921459309102	21.832330558864257
80-81	21.567968013827358	28.443292437001038	28.14903545066762	21.839704098503987
82-83	21.723773337933846	28.287639044215474	28.26273338088906	21.725854236961617
84-85	21.691712656822286	28.332155016078346	28.161295875274394	21.814836451824966
86-87	21.508022493823237	28.48397186883541	28.31820322422397	21.689802413117388
88-89	21.68020767881067	28.34768840363956	28.232312717031444	21.739791200518326
90-91	21.66467253802645	28.386004151744164	28.286456048683313	21.662867261546072
92-93	21.737865470538075	28.42113319915825	28.175770201807588	21.66523112849609
94-95	21.500960033772813	28.42210319182343	28.316640623573587	21.760296150830168
96-97	21.5644394290772	28.55098226575803	28.396440110306287	21.48813819485848
98-99	22.46368445196469	30.484562108274304	28.650632660598337	18.401120779162667
100	21.697082103823902	31.409860590407845	22.32496096115424	24.568096344614013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4701.0
1	4829.0
2	8082.5
3	11373.0
4	14225.5
5	24433.5
6	33431.0
7	38190.0
8	39509.0
9	35508.0
10	29361.5
11	22942.0
12	23311.5
13	32731.0
14	58249.5
15	96965.0
16	129378.0
17	146835.5
18	151052.0
19	144563.0
20	130306.0
21	115432.0
22	107978.0
23	113826.0
24	133506.0
25	167095.0
26	214075.0
27	265447.5
28	315718.5
29	372978.5
30	432946.0
31	489654.0
32	555555.5
33	623774.5
34	688501.5
35	751338.5
36	817091.0
37	870989.0
38	894677.0
39	908732.0
40	921364.0
41	921022.5
42	909039.0
43	909852.5
44	913617.0
45	909395.5
46	899463.0
47	880197.0
48	859322.0
49	830624.5
50	797856.5
51	755325.0
52	702213.5
53	648633.5
54	591768.0
55	537296.5
56	485180.5
57	430672.0
58	380377.5
59	332476.0
60	280205.5
61	223979.5
62	173457.5
63	135683.0
64	105240.5
65	80564.0
66	60945.0
67	46716.0
68	36911.5
69	28388.5
70	21160.0
71	15683.5
72	11830.0
73	8960.0
74	6603.5
75	4828.5
76	3540.5
77	2565.0
78	1892.0
79	1386.5
80	1000.5
81	736.0
82	577.0
83	421.0
84	313.5
85	242.5
86	193.5
87	160.0
88	113.5
89	74.0
90	52.5
91	41.0
92	29.0
93	15.5
94	7.5
95	4.5
96	2.5
97	3.0
98	4.5
99	5.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013247517483318342
3	0.0
4	0.12789901944085094
5	0.036299721702133615
6	0.004590440997095212
7	0.005109484636808261
8	4.333300111365813E-4
9	6.428522143234998E-4
10-11	0.004735677978849781
12-13	3.809494603398517E-5
14-15	5.4761484923853676E-5
16-17	3.809494603398517E-4
18-19	1.4285604762744436E-5
20-21	9.150965115829891E-4
22-23	0.030287498363821334
24-25	0.0010224908570085954
26-27	0.004524117397568815
28-29	5.848175235106232E-4
30-31	0.0026797803983653386
32-33	0.0033900069303323174
34-35	0.019730225284753933
36-37	0.004322141992139845
38-39	0.0016507298768474448
40-41	0.0010063368984924825
42-43	0.0011232079298780038
44-45	0.030463448831155784
46-47	0.003918292085626206
48-49	0.005502645129804134
50-51	0.019445936605461393
52-53	0.004256093280460907
54-55	9.729298658041834E-4
56-57	0.0012834876594948676
58-59	0.0039701473182809765
60-61	0.0029131996113390844
62-63	7.738344548431955E-4
64-65	1.2323607514274867E-4
66-67	2.830057588841875E-6
68-69	2.889891334649388E-4
70-71	0.01335077541315179
72-73	0.004280308190387288
74-75	0.002991311438570786
76-77	2.990665743428214E-6
78-79	0.0
80-81	7.962237922938766E-5
82-83	3.1011907052724804E-6
84-85	1.5694153836669748E-5
86-87	0.005712765880718656
88-89	0.0
90-91	6.529024599308047E-5
92-93	8.618525308001234E-5
94-95	2.459114111889086E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	12828.0
20-21	24997.0
22-23	26360.0
24-25	28815.0
26-27	34842.0
28-29	49473.0
30-31	78282.0
32-33	90364.0
34-35	110932.0
36-37	144091.0
38-39	182094.0
40-41	181378.0
42-43	206365.0
44-45	244774.0
46-47	232616.0
48-49	203269.0
50-51	180997.0
52-53	167596.0
54-55	172859.0
56-57	181161.0
58-59	187801.0
60-61	187428.0
62-63	174341.0
64-65	181934.0
66-67	191070.0
68-69	199543.0
70-71	199960.0
72-73	181081.0
74-75	178303.0
76-77	186606.0
78-79	199221.0
80-81	206611.0
82-83	195319.0
84-85	191573.0
86-87	197768.0
88-89	213634.0
90-91	231652.0
92-93	237281.0
94-95	263199.0
96-97	643284.0
98-99	1257944.0
100-101	1.2740515E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.98241034335805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.21717710060854	74.03456399838745
2	7.226556367770404	11.993541317594614
3	1.8451351273205816	4.593412808227821
4	0.7563315429530721	2.5104885781182786
5	0.35255817864184186	1.4628063724982125
6	0.18785476186308653	0.9353184560325871
7	0.11766931127244824	0.6835138150981438
8	0.07130039750615225	0.4733343074800055
9	0.04725054967084918	0.3528868051562065
>10	0.1714071735688413	2.215524865522203
>50	0.004353054725022716	0.2454542141124188
>100	0.002179924560676224	0.33585870323858275
>500	1.6701140789845308E-4	0.0879921478435317
>1k	5.949813063412334E-5	0.07530361068981133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.761868254248146E-6	0.0
2	0.0	2.380934127124073E-5	0.0	2.380934127124073E-5	0.0
3	0.0	2.380934127124073E-5	0.0	2.380934127124073E-5	4.761868254248146E-6
4	0.0	5.714241905097775E-5	0.0	3.3333077779737025E-5	4.761868254248146E-6
5	4.761868254248146E-6	6.19042873052259E-5	0.0	3.3333077779737025E-5	4.761868254248146E-6
6	1.4285604762744438E-5	6.19042873052259E-5	0.0	3.3333077779737025E-5	9.523736508496293E-6
7	1.4285604762744438E-5	6.19042873052259E-5	0.0	3.3333077779737025E-5	9.523736508496293E-6
8	1.4285604762744438E-5	6.666615555947405E-5	0.0	3.3333077779737025E-5	9.523736508496293E-6
9	1.4285604762744438E-5	1.238085746104518E-4	0.0	6.666615555947405E-5	9.523736508496293E-6
10-11	1.4285604762744438E-5	1.238085746104518E-4	0.0	9.285643095783885E-5	9.523736508496293E-6
12-13	1.4285604762744438E-5	1.2618950873757585E-4	0.0	1.0238016746633513E-4	1.1904670635620365E-5
14-15	1.9047473016992585E-5	2.142840714411666E-4	0.0	1.1666577222907957E-4	1.9047473016992585E-5
16-17	3.3333077779737025E-5	2.2380780794966287E-4	0.0	1.4285604762744438E-4	1.9047473016992585E-5
18-19	3.3333077779737025E-5	2.3333154445815915E-4	0.0	1.4761791588169254E-4	2.1428407144116658E-5
20-21	3.3333077779737025E-5	2.4999808334802767E-4	0.0	1.7142725715293326E-4	2.380934127124073E-5
22-23	3.5714011906861094E-5	2.7856929287351656E-4	0.0	1.928556642970499E-4	2.380934127124073E-5
24-25	3.809494603398517E-5	3.047595682718813E-4	0.0	2.452362150937795E-4	2.380934127124073E-5
26-27	3.809494603398517E-5	3.166642389075017E-4	0.0	3.095214365261295E-4	2.380934127124073E-5
28-29	3.809494603398517E-5	3.190451730346258E-4	0.0	4.88091496060435E-4	2.380934127124073E-5
30-31	3.809494603398517E-5	3.6904478970423133E-4	0.0	0.0012095145365790291	2.6190275398364803E-5
32-33	3.809494603398517E-5	3.714257238313554E-4	0.0	0.0027214077073028156	2.8571209525488876E-5
34-35	3.809494603398517E-5	4.1190160399246466E-4	0.0	0.004511870170900118	3.3333077779737025E-5
36-37	3.809494603398517E-5	4.4761561589932574E-4	0.0	0.007361848321067634	3.3333077779737025E-5
38-39	3.809494603398517E-5	4.595202865349461E-4	0.0	0.012014193605468073	3.3333077779737025E-5
40-41	3.809494603398517E-5	5.380911127300405E-4	0.0	0.01731653390657338	3.3333077779737025E-5
42-43	6.19042873052259E-5	5.666623222555294E-4	0.0	0.021899832101287223	3.3333077779737025E-5
44-45	6.19042873052259E-5	6.357094119421275E-4	0.0	0.027202172402392533	3.3333077779737025E-5
46-47	6.19042873052259E-5	6.809471603574849E-4	0.0	0.032768796391608616	3.809494603398517E-5
48-49	6.19042873052259E-5	6.833280944846089E-4	0.0	0.038361610656223066	3.809494603398517E-5
50-51	6.19042873052259E-5	7.976129325865645E-4	0.0	0.04407347162719372	4.2856814288233316E-5
52-53	6.19042873052259E-5	7.999938667136885E-4	0.0	0.04965914308942679	4.999961666960554E-5
54-55	6.666615555947405E-5	8.118985373493088E-4	0.0	0.05489005536671838	6.19042873052259E-5
56-57	7.618989206797034E-5	8.357078786205496E-4	0.0	0.059909064506695926	7.380895794084627E-5
58-59	7.618989206797034E-5	8.428506810019219E-4	0.0	0.06501140634112282	8.333269444934255E-5
60-61	7.618989206797034E-5	8.547553516375422E-4	0.0	0.07018755713349054	9.047549683071477E-5
62-63	7.618989206797034E-5	8.595172198917903E-4	0.0	0.07511370984251026	9.523736508496292E-5
64-65	7.857082619509441E-5	9.285643095783884E-4	0.0	0.07977319792929206	9.523736508496292E-5
66-67	8.095176032221848E-5	9.476117825953811E-4	0.0	0.08431840117797193	9.999923333921106E-5
68-69	8.095176032221848E-5	9.595164532310014E-4	0.0	0.08879455733696517	9.999923333921106E-5
70-71	8.095176032221848E-5	9.690401897394978E-4	0.0	0.09302547728086466	9.999923333921106E-5
72-73	8.333269444934255E-5	9.738020579937458E-4	0.0	0.09626354769375339	1.142848381019555E-4
74-75	8.571362857646663E-5	9.928495310107385E-4	0.0	0.09887781336533563	1.142848381019555E-4
76-77	8.571362857646663E-5	0.0010523728841888402	0.0	0.10118731946864598	1.142848381019555E-4
78-79	8.571362857646663E-5	0.001076182225460081	0.0	0.10262064181317468	1.142848381019555E-4
80-81	8.571362857646663E-5	0.001076182225460081	0.0	0.1031777803989217	1.1904670635620366E-4
82-83	8.571362857646663E-5	0.001078563159587205	0.0	0.10363015788307528	1.333323111189481E-4
84-85	9.047549683071477E-5	0.0010976106326041975	0.0	0.10374920458943149	1.3571324524607218E-4
86-87	9.047549683071477E-5	0.00110475343498557	0.0	0.1038015851402282	1.3809417937319624E-4
88	9.047549683071477E-5	0.001171419590545044	0.0	0.10380872794260958	1.3809417937319624E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	32725	0.0	51.15712	1
GTATCAA	61150	0.0	37.115555	1
TCAACGC	78315	0.0	28.509336	4
ATCAACG	78615	0.0	28.389992	3
CAACGCA	78910	0.0	28.25023	5
TATCAAC	80995	0.0	27.719604	2
AACGCAG	81590	0.0	27.43905	6
ACGCAGA	92435	0.0	24.09406	7
CGCAGAG	93675	0.0	23.788267	8
GCAGAGT	105445	0.0	20.744307	9
GTACATG	80100	0.0	19.96844	1
TACATGG	79410	0.0	19.59884	2
ACATGGG	79145	0.0	19.308165	3
AGAGTAC	98815	0.0	18.665506	10-11
GAGTACT	60125	0.0	18.38159	12-13
CAGAGTA	104730	0.0	18.095512	10-11
CTTATAC	13435	0.0	17.779184	1
CATGGGG	46915	0.0	17.298407	4
AGTACTT	65760	0.0	16.844234	12-13
GTACTTT	66515	0.0	16.569029	14-15
>>END_MODULE