FastQCFastQC Report
Fri 27 May 2016
SRR2139665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20917593
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA366150.050.7978321
GTATCAA671500.036.465731
CTTATAC160950.028.27131
ATCAACG865500.027.8507693
TCAACGC871350.027.6780784
CAACGCA873000.027.5592885
TATCAAC888200.027.3959982
AACGCAG904600.026.7302386
TATACAC226600.023.7083643
ACGCAGA1016450.023.674267
CGCAGAG1026650.023.422738
GCAGAGT1155500.020.480819
GTACATG860650.020.221851
TACATGG855550.019.90082
ACATGGG850300.019.6770533
TTATACA255450.018.9341662
TATGCCG121850.018.77248642-43
ACATCTC241200.018.6985828
AGAGTAC1074800.018.69591310-11
ACACATC246750.018.5808436
Produced by FastQC (version 0.11.5)