##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139665_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20917593
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.531560347311473	31.0	31.0	34.0	28.0	34.0
2	31.556468997173816	31.0	31.0	34.0	28.0	34.0
3	31.641631998480896	33.0	31.0	34.0	28.0	34.0
4	35.195392079767494	37.0	35.0	37.0	32.0	37.0
5	35.02933702744862	37.0	35.0	37.0	32.0	37.0
6	35.08040633547082	37.0	35.0	37.0	32.0	37.0
7	34.93629725944089	37.0	35.0	37.0	32.0	37.0
8	34.95870060192872	37.0	35.0	37.0	32.0	37.0
9	36.45798175727006	39.0	37.0	39.0	32.0	39.0
10-11	36.395095745480845	39.0	35.0	39.0	31.5	39.0
12-13	36.37913728888405	39.0	35.0	39.0	31.0	39.0
14-15	37.532023641534664	40.0	37.0	41.0	31.0	41.0
16-17	37.55925999707519	40.0	37.0	41.0	31.0	41.0
18-19	37.63356876195076	40.0	37.0	41.0	31.0	41.0
20-21	37.665336363972344	40.0	37.0	41.0	31.0	41.0
22-23	37.62803623943515	40.0	37.0	41.0	31.0	41.0
24-25	37.62088456642326	40.0	37.0	41.0	31.0	41.0
26-27	37.546842994824395	40.0	37.0	41.0	31.0	41.0
28-29	37.48648379121329	40.0	36.5	41.0	31.0	41.0
30-31	37.38410667680752	40.0	36.0	41.0	31.0	41.0
32-33	37.296496854621665	40.0	36.0	41.0	30.5	41.0
34-35	37.20712202877901	40.0	36.0	41.0	30.0	41.0
36-37	37.17870396934626	40.0	36.0	41.0	30.0	41.0
38-39	37.113764033894725	40.0	36.0	41.0	30.0	41.0
40-41	37.10572577044904	40.0	35.0	41.0	30.0	41.0
42-43	37.077665362806314	40.0	35.0	41.0	30.0	41.0
44-45	37.011668721540815	39.5	35.0	41.0	30.0	41.0
46-47	36.94561901562069	39.0	35.0	41.0	30.0	41.0
48-49	36.88789815568085	39.0	35.0	41.0	30.0	41.0
50-51	36.81439641571117	39.0	35.0	41.0	30.0	41.0
52-53	37.04176923279658	39.0	35.0	41.0	30.0	41.0
54-55	37.033228965487666	39.0	35.0	41.0	30.0	41.0
56-57	36.9586186744729	39.0	35.0	41.0	30.0	41.0
58-59	36.82931893647229	39.0	35.0	41.0	30.0	41.0
60-61	36.7092831834206	39.0	35.0	41.0	30.0	41.0
62-63	36.55405963552505	38.5	35.0	41.0	30.0	41.0
64-65	36.36950072070125	38.0	35.0	40.0	29.5	41.0
66-67	36.1555265092457	37.5	34.0	40.0	29.0	41.0
68-69	35.9085301228793	37.0	34.0	40.0	29.0	41.0
70-71	35.67633606477721	37.0	34.0	39.5	29.0	41.0
72-73	35.406986354600264	36.0	34.0	39.0	29.0	41.0
74-75	35.1457070794566	36.0	34.0	39.0	29.0	40.5
76-77	34.85715770338409	35.5	34.0	38.5	29.0	40.0
78-79	34.58241826123603	35.0	34.0	37.0	29.0	39.0
80-81	34.32322938099045	35.0	33.5	37.0	29.0	39.0
82-83	34.05349369791193	35.0	33.0	36.5	29.0	39.0
84-85	33.83215466448013	35.0	33.0	36.0	29.0	37.0
86-87	33.609746151731315	35.0	33.0	36.0	29.0	37.0
88-89	33.46632698051637	35.0	33.0	35.0	29.0	37.0
90-91	33.36378774551292	35.0	33.0	35.0	29.0	36.0
92-93	33.291266338110376	35.0	33.0	35.0	29.0	36.0
94-95	33.34440797029911	35.0	33.0	35.0	29.0	36.0
96-97	33.528152968014865	35.0	33.0	35.0	30.0	36.0
98-99	33.62975713667841	35.0	34.0	35.0	30.0	35.5
100	32.826107175051646	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	21.0
15	29.0
16	72.0
17	327.0
18	2468.0
19	12080.0
20	36914.0
21	79750.0
22	132053.0
23	180130.0
24	223560.0
25	263168.0
26	298823.0
27	333615.0
28	376017.0
29	426512.0
30	491221.0
31	574294.0
32	686416.0
33	843540.0
34	1084303.0
35	1436216.0
36	2090891.0
37	3506939.0
38	4849392.0
39	2852804.0
40	136031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.86092472494326	21.89389572691275	14.497079085533407	23.748100462610587
2	14.130742966866253	22.765770671955394	41.27697773791258	21.826508623265774
3	16.402604257573994	29.201208762403972	31.970093308536978	22.426093671485052
4	11.489982673005953	20.007182962392314	40.5036555754694	27.99917878913233
5	11.395955751717226	39.86178509138367	36.76080896443313	11.98145019246597
6	26.823927934564306	41.23210989213047	19.581154866191095	12.362807307114133
7	23.893719761032823	34.41189743242576	25.115280926605692	16.579101879935724
8	23.716141098089853	37.10777647716589	22.20625739064004	16.96982503410421
9	25.651184122593023	15.868615283367667	21.804892187970527	36.67530840606879
10-11	23.02758646902137	27.412881972424287	30.355256197292153	19.204275361262194
12-13	24.356114202940468	25.420276119318252	30.066158442028744	20.157451235712532
14-15	21.64014735162531	26.40985928394025	27.849545148235094	24.100448216199347
16-17	20.56322079543288	29.394247863847696	28.49242711228236	21.550104228437068
18-19	20.57086312793657	28.500811649488178	30.490096886646374	20.438228335928876
20-21	21.88718426268952	27.47990252769298	30.170143465272346	20.46276974434515
22-23	21.48005695172165	27.567420470937343	29.95854439988717	20.993978177453837
24-25	21.060024220583657	27.815153400028912	30.18850907852016	20.936313300867273
26-27	20.98112458107175	27.908314216567643	30.090208960411953	21.020352241948657
28-29	20.889420117507726	27.70679252614594	30.319505024114648	21.084282332231684
30-31	21.276739564285453	27.67978618370727	29.92269633566078	21.12077791634649
32-33	21.13739862469533	27.967807533876478	29.923001726751103	20.971792114677086
34-35	21.197741539827042	28.11343756218395	29.89767314875135	20.791147749237663
36-37	21.201130509216817	27.881944469971494	29.70337488809963	21.213550132712058
38-39	21.040537878868697	27.984336149302052	29.877916574309054	21.097209397520192
40-41	21.234284190483784	27.85085183376033	29.817112438068843	21.09775153768704
42-43	21.2971565908068	27.94314046939077	29.402220083057156	21.357482856745268
44-45	21.253670724336935	28.30208729467629	29.13352537398215	21.310716607004622
46-47	21.550328404245178	28.102909261299285	28.686494038463582	21.66026829599195
48-49	21.522304513176874	28.2403519437352	28.614266494326724	21.623077048761203
50-51	21.44057718886887	28.338329201091916	28.454910858489875	21.76618275154934
52-53	21.5258233383228	28.315432454917733	28.53675797608295	21.621986230676516
54-55	21.573118526347187	28.253698939358095	28.518248693697863	21.654933840596854
56-57	21.70569681366748	28.401996848588873	28.27960712271019	21.612699215033462
58-59	21.445074151613287	28.52751886891356	28.55566650383159	21.47174047564156
60-61	21.68444178619425	28.73726888817914	28.190892120668686	21.38739720495792
62-63	21.4838250929735	28.83416497011872	28.228062227308715	21.453947709599067
64-65	21.40472733525361	28.635504205634067	28.318151384850864	21.64161707426146
66-67	21.66632553184045	28.68678377471015	28.08926765811145	21.557623035337944
68-69	21.558308985376996	28.59381171377865	28.252947975047928	21.594931325796427
70-71	21.612867542575962	28.203445889025602	28.40668580968831	21.777000758710123
72-73	21.709072627119877	28.293621013353416	28.271857689398722	21.725448670127985
74-75	21.474045739608723	28.464290033671052	28.303594081265555	21.75807014545467
76-77	21.539406714215414	28.442672707441098	28.290744396938265	21.727176181405223
78-79	21.526419739879536	28.437318631349505	28.212675632621114	21.82358599614984
80-81	21.53607002043093	28.468219516945144	28.207275465520397	21.78843499710353
82-83	21.71593197601027	28.279918693568373	28.377699779108173	21.626449551313183
84-85	21.685033113495773	28.342044222974128	28.19025510410299	21.782667559427107
86-87	21.50598889293223	28.527708604115375	28.30817011608402	21.658132386868374
88-89	21.669498206196156	28.362260570440178	28.27700480104672	21.691236422316948
90-91	21.648203370387183	28.396158035284184	28.344998438903872	21.610640155424754
92-93	21.719756563159972	28.427021337437363	28.22631118406582	21.626910915336843
94-95	21.447248533388354	28.402331563793876	28.393366227904803	21.757053674912967
96-97	21.51258990007712	28.611540546561017	28.41919639430363	21.456673159058237
98-99	22.424754931827785	30.550276077409805	28.675441605254765	18.34952738550765
100	21.671194683143426	31.417016482083902	22.418705183451895	24.493083651320777
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5564.0
1	5741.0
2	9194.0
3	12577.0
4	15841.5
5	27583.5
6	37737.5
7	42690.5
8	43835.0
9	39116.5
10	31957.5
11	24478.5
12	24795.5
13	35484.5
14	64324.0
15	107871.0
16	144309.0
17	163504.5
18	167257.5
19	158754.5
20	141565.0
21	123263.0
22	112367.5
23	115195.0
24	132588.5
25	165206.5
26	211804.5
27	263531.0
28	313624.5
29	370400.5
30	428879.5
31	484457.0
32	549308.5
33	614799.5
34	678644.0
35	738953.5
36	801434.0
37	855797.5
38	878482.5
39	888804.5
40	898728.5
41	896720.0
42	884249.5
43	888917.5
44	899728.0
45	904267.0
46	904209.5
47	892445.0
48	871814.5
49	838819.5
50	801795.5
51	756053.0
52	702568.5
53	648153.0
54	589669.5
55	531679.5
56	476915.0
57	422956.5
58	372863.0
59	328149.0
60	279122.5
61	222870.0
62	172070.0
63	133911.5
64	104038.5
65	80145.5
66	61041.0
67	47397.0
68	37423.0
69	28648.5
70	21737.5
71	16470.5
72	12643.5
73	9706.5
74	7091.0
75	5190.0
76	3854.0
77	2765.0
78	2015.0
79	1473.0
80	1074.5
81	787.5
82	608.0
83	416.0
84	290.5
85	235.5
86	195.5
87	157.5
88	107.0
89	73.5
90	57.5
91	45.5
92	32.5
93	16.5
94	6.5
95	5.0
96	4.5
97	4.0
98	3.0
99	3.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012730910291638241
3	0.0
4	0.1266445905128759
5	0.035391261317686024
6	0.004474702227928423
7	0.005163117955302028
8	4.2069850006164666E-4
9	5.83241102358192E-4
10-11	0.004771103443880947
12-13	2.6293656253852916E-5
14-15	4.302598296085023E-5
16-17	3.6811118755394087E-4
18-19	2.1512991480425115E-5
20-21	8.92455349956387E-4
22-23	0.030486858965873765
24-25	0.0010724601073630936
26-27	0.004645196726003828
28-29	6.212129037477558E-4
30-31	0.0025791292178754424
32-33	0.003423849619869036
34-35	0.020084899386202164
36-37	0.00439862738320756
38-39	0.001701425421621986
40-41	0.0011289535898643195
42-43	0.0010363674415609504
44-45	0.030723031968535548
46-47	0.0038790809586605104
48-49	0.005701371489927061
50-51	0.019501513104711436
52-53	0.004406342083485867
54-55	0.0011035731233444395
56-57	0.001348517259331895
58-59	0.004173685209368008
60-61	0.0029590342373485997
62-63	9.097509527554646E-4
64-65	1.7694656458104433E-4
66-67	0.0
68-69	2.8113577245582024E-4
70-71	0.013390794371582703
72-73	0.0044451894497198755
74-75	0.003061261381935772
76-77	2.9932483392485408E-6
78-79	0.0
80-81	5.509213716142476E-5
82-83	3.097670991283835E-6
84-85	1.2532901215609952E-5
86-87	0.005571828909101507
88-89	0.0
90-91	9.43063117133187E-5
92-93	1.1220404080412147E-4
94-95	2.41309393028451E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	13806.0
20-21	26877.0
22-23	29552.0
24-25	32078.0
26-27	37678.0
28-29	53179.0
30-31	81865.0
32-33	92582.0
34-35	113288.0
36-37	143634.0
38-39	185013.0
40-41	182850.0
42-43	205816.0
44-45	246337.0
46-47	233076.0
48-49	201127.0
50-51	174070.0
52-53	158416.0
54-55	162327.0
56-57	171128.0
58-59	178562.0
60-61	179946.0
62-63	168557.0
64-65	179266.0
66-67	189196.0
68-69	196887.0
70-71	196350.0
72-73	171051.0
74-75	166060.0
76-77	173904.0
78-79	187357.0
80-81	197567.0
82-83	185163.0
84-85	181437.0
86-87	186363.0
88-89	204484.0
90-91	223135.0
92-93	229235.0
94-95	254127.0
96-97	634634.0
98-99	1260228.0
100-101	1.2829385E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.05034267560212
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.55741627792153	71.77608935791486
2	7.341766950936285	11.90105454435593
3	2.049390552797074	4.983114197410336
4	0.8377006116234755	2.7158368652657674
5	0.4370721316961112	1.7712423023962858
6	0.2391245736831754	1.1628677183507183
7	0.14357068397515782	0.8145517194049984
8	0.09366011200969068	0.6072947338736567
9	0.06585897258789747	0.4804103066860959
>10	0.22561751054927986	2.836408563205057
>50	0.005443437881926495	0.3088951671919896
>100	0.0030979700459506476	0.458607313439838
>500	2.2690381437590968E-4	0.11629792597537127
>1k	5.331047810356548E-5	0.06732928452897942
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.561329546855606E-6
2	0.0	1.434199432028341E-5	0.0	0.0	9.561329546855606E-6
3	0.0	1.434199432028341E-5	0.0	4.780664773427803E-6	9.561329546855606E-6
4	0.0	2.3903323867139014E-5	0.0	9.561329546855606E-6	9.561329546855606E-6
5	9.561329546855606E-6	2.3903323867139014E-5	0.0	1.434199432028341E-5	9.561329546855606E-6
6	9.561329546855606E-6	2.3903323867139014E-5	0.0	1.434199432028341E-5	2.3903323867139014E-5
7	9.561329546855606E-6	2.3903323867139014E-5	0.0	1.434199432028341E-5	2.3903323867139014E-5
8	1.434199432028341E-5	2.3903323867139014E-5	0.0	1.434199432028341E-5	2.868398864056682E-5
9	1.434199432028341E-5	5.736797728113364E-5	0.0	1.434199432028341E-5	3.346465341399462E-5
10-11	1.434199432028341E-5	5.736797728113364E-5	0.0	3.107432102728072E-5	3.346465341399462E-5
12-13	2.151299148042511E-5	5.736797728113364E-5	0.0	3.346465341399462E-5	3.346465341399462E-5
14-15	2.3903323867139014E-5	8.605196592170046E-5	0.0	4.302598296085022E-5	3.8245318187422425E-5
16-17	2.3903323867139014E-5	8.605196592170046E-5	0.0	6.692930682798924E-5	4.302598296085023E-5
18-19	3.107432102728072E-5	9.561329546855607E-5	0.0	7.170997160141704E-5	4.302598296085023E-5
20-21	4.780664773427803E-5	1.0517462501541167E-4	0.0	1.0039396024198385E-4	4.302598296085023E-5
22-23	5.019698012099193E-5	1.2429728410912288E-4	0.0	1.0995528978883948E-4	5.0196980120991934E-5
24-25	5.258731250770583E-5	1.3624894604269237E-4	0.0	1.45810275589548E-4	6.692930682798924E-5
26-27	5.258731250770583E-5	1.482006079762619E-4	0.0	1.840555937769704E-4	6.692930682798924E-5
28-29	5.258731250770583E-5	1.5059094036297578E-4	0.0	2.987915483392377E-4	6.692930682798924E-5
30-31	5.258731250770583E-5	1.7210393184340092E-4	0.0	0.0010087202671932665	6.692930682798924E-5
32-33	5.258731250770583E-5	1.7210393184340092E-4	0.0	0.0024094550458076125	8.127130114827265E-5
34-35	5.736797728113364E-5	2.0556858525739555E-4	0.0	0.004128104031854908	8.605196592170046E-5
36-37	5.736797728113364E-5	2.3903323867139015E-4	0.0	0.006535168745275807	8.605196592170046E-5
38-39	6.214864205456144E-5	2.5337523299167355E-4	0.0	0.010866451030001396	8.605196592170046E-5
40-41	6.692930682798924E-5	3.011818807259516E-4	0.0	0.015529989516480219	8.605196592170046E-5
42-43	6.692930682798924E-5	3.131335426595211E-4	0.0	0.019629409559694562	8.605196592170046E-5
44-45	6.692930682798924E-5	3.465981960735157E-4	0.0	0.02414474743819712	8.605196592170046E-5
46-47	6.692930682798924E-5	3.681111875539408E-4	0.0	0.029056880492894187	8.844229830841437E-5
48-49	6.692930682798924E-5	3.7050151994065474E-4	0.0	0.03402638152487239	9.561329546855606E-5
50-51	6.692930682798924E-5	4.183081676749328E-4	0.0	0.03919667047733456	9.561329546855606E-5
52-53	6.931963921470315E-5	4.254791648350745E-4	0.0	0.04418051350363304	1.0756495740212556E-4
54-55	7.170997160141705E-5	4.254791648350745E-4	0.0	0.04878429368044401	1.2429728410912288E-4
56-57	7.170997160141705E-5	4.326501619952162E-4	0.0	0.053041475661181475	1.338586136559785E-4
58-59	7.410030398813096E-5	4.3504049438193007E-4	0.0	0.05738470960784063	1.386392784294063E-4
60-61	8.844229830841437E-5	4.541631534756413E-4	0.0	0.0623135749892447	1.482006079762619E-4
62-63	1.0039396024198387E-4	4.685051477959247E-4	0.0	0.06676198356091927	1.673232670699731E-4
64-65	1.0039396024198387E-4	5.210924603036305E-4	0.0	0.07094267490528189	1.673232670699731E-4
66-67	1.0517462501541167E-4	5.210924603036305E-4	0.0	0.07492496866154724	1.673232670699731E-4
68-69	1.0517462501541167E-4	5.234827926903444E-4	0.0	0.07895028840077345	1.673232670699731E-4
70-71	1.0517462501541167E-4	5.258731250770584E-4	0.0	0.08290628850078496	1.8644592616368433E-4
72-73	1.0995528978883948E-4	5.258731250770584E-4	0.0	0.08588703298701719	2.0556858525739555E-4
74-75	1.1473595456226728E-4	5.426054517840557E-4	0.0	0.08829170736805139	2.0556858525739555E-4
76-77	1.1473595456226728E-4	5.56947446104339E-4	0.0	0.09051471648769531	2.0556858525739555E-4
78-79	1.1473595456226728E-4	5.641184432644807E-4	0.0	0.09199672256745793	2.0556858525739555E-4
80-81	1.1473595456226728E-4	5.641184432644807E-4	0.0	0.09247000838002728	2.1273958241753725E-4
82-83	1.1473595456226728E-4	5.641184432644807E-4	0.0	0.09277836125791337	2.2947190912453455E-4
84-85	1.1473595456226728E-4	5.688991080379086E-4	0.0	0.09286202289144836	2.2947190912453455E-4
86-87	1.1473595456226728E-4	5.784604375847641E-4	0.0	0.09287636488576864	2.2947190912453455E-4
88	1.1473595456226728E-4	5.975830966784754E-4	0.0	0.09287875521815536	2.3425257389796236E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	36615	0.0	50.797832	1
GTATCAA	67150	0.0	36.46573	1
CTTATAC	16095	0.0	28.2713	1
ATCAACG	86550	0.0	27.850769	3
TCAACGC	87135	0.0	27.678078	4
CAACGCA	87300	0.0	27.559288	5
TATCAAC	88820	0.0	27.395998	2
AACGCAG	90460	0.0	26.730238	6
TATACAC	22660	0.0	23.708364	3
ACGCAGA	101645	0.0	23.67426	7
CGCAGAG	102665	0.0	23.42273	8
GCAGAGT	115550	0.0	20.48081	9
GTACATG	86065	0.0	20.22185	1
TACATGG	85555	0.0	19.9008	2
ACATGGG	85030	0.0	19.677053	3
TTATACA	25545	0.0	18.934166	2
TATGCCG	12185	0.0	18.772486	42-43
ACATCTC	24120	0.0	18.698582	8
AGAGTAC	107480	0.0	18.695913	10-11
ACACATC	24675	0.0	18.580843	6
>>END_MODULE