FastQCFastQC Report
Fri 27 May 2016
SRR2139664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14042414
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA253750.049.4849471
GTATCAA471150.037.3486941
ATCAACG618600.028.0199683
TCAACGC618650.028.0110424
CAACGCA620250.027.9032555
TATCAAC634700.027.516882
AACGCAG642300.027.0832716
CTTATAC104500.025.1205941
ACGCAGA728200.023.763837
CGCAGAG734550.023.5471978
TATACAC164700.021.1682533
GCAGAGT814200.020.8141449
GTACATG616850.020.2561231
TACATGG616300.019.866312
ACATGGG609250.019.6836533
AGAGTAC770650.018.79238110-11
TATGCCG71950.018.46345542-43
GAGTACT464650.018.44294512-13
CAGAGTA812500.017.81176810-11
CTCGTAT70200.017.78198638-39
Produced by FastQC (version 0.11.5)