##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14042414
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54438140051988	31.0	31.0	34.0	28.0	34.0
2	31.571354184544052	31.0	31.0	34.0	28.0	34.0
3	31.653986914215746	33.0	31.0	34.0	28.0	34.0
4	35.209745845692915	37.0	35.0	37.0	32.0	37.0
5	35.047257757818564	37.0	35.0	37.0	32.0	37.0
6	35.098658464278294	37.0	35.0	37.0	32.0	37.0
7	34.958996793571245	37.0	35.0	37.0	32.0	37.0
8	34.982695710295964	37.0	35.0	37.0	32.0	37.0
9	36.484867843947626	39.0	37.0	39.0	32.0	39.0
10-11	36.42495919861072	39.0	36.0	39.0	32.0	39.0
12-13	36.410414477168956	39.0	35.0	39.0	31.5	39.0
14-15	37.56125837765501	40.0	37.0	41.0	31.0	41.0
16-17	37.59078684761751	40.0	37.0	41.0	31.0	41.0
18-19	37.669495002782284	40.0	37.0	41.0	31.0	41.0
20-21	37.700431442120305	40.0	37.0	41.0	31.0	41.0
22-23	37.65973402855761	40.0	37.0	41.0	31.0	41.0
24-25	37.65367521430689	40.0	37.0	41.0	31.0	41.0
26-27	37.57851409365027	40.0	37.0	41.0	31.0	41.0
28-29	37.5209837808131	40.0	37.0	41.0	31.0	41.0
30-31	37.41373593820725	40.0	36.0	41.0	31.0	41.0
32-33	37.325475947720804	40.0	36.0	41.0	31.0	41.0
34-35	37.23880233587893	40.0	36.0	41.0	30.0	41.0
36-37	37.2096424140456	40.0	36.0	41.0	30.0	41.0
38-39	37.1450775840259	40.0	36.0	41.0	30.0	41.0
40-41	37.13821306461182	40.0	35.5	41.0	30.0	41.0
42-43	37.11325360094698	40.0	35.0	41.0	30.0	41.0
44-45	37.05338144054323	40.0	35.0	41.0	30.0	41.0
46-47	36.98817234757513	39.5	35.0	41.0	30.0	41.0
48-49	36.93985893489668	39.0	35.0	41.0	30.0	41.0
50-51	36.874116535302505	39.0	35.0	41.0	30.0	41.0
52-53	37.104126686040956	39.0	35.0	41.0	30.0	41.0
54-55	37.10606154457196	39.0	35.0	41.0	30.0	41.0
56-57	37.03685977703157	39.0	35.0	41.0	30.0	41.0
58-59	36.91508310943623	39.0	35.0	41.0	30.0	41.0
60-61	36.80666355841116	39.0	35.0	41.0	30.0	41.0
62-63	36.65951708018146	39.0	35.0	41.0	30.0	41.0
64-65	36.48057506326789	38.0	35.0	40.5	30.0	41.0
66-67	36.27378570073711	38.0	34.5	40.0	29.5	41.0
68-69	36.03428597174705	37.0	34.0	40.0	29.0	41.0
70-71	35.80367551324464	37.0	34.0	40.0	29.0	41.0
72-73	35.53955421399411	36.5	34.0	39.0	29.0	41.0
74-75	35.27818280291228	36.0	34.0	39.0	29.0	41.0
76-77	34.9882647051248	36.0	34.0	38.5	29.0	40.0
78-79	34.70920975849996	35.0	34.0	37.5	29.0	39.5
80-81	34.442418081172875	35.0	34.0	37.0	29.0	39.0
82-83	34.16510705700439	35.0	33.5	36.5	29.0	39.0
84-85	33.93435781148675	35.0	33.0	36.0	29.0	37.5
86-87	33.70048925185908	35.0	33.0	36.0	29.0	37.0
88-89	33.548433790530396	35.0	33.0	35.5	29.0	37.0
90-91	33.43795308384272	35.0	33.0	35.0	29.0	36.0
92-93	33.35677030807895	35.0	33.0	35.0	29.0	36.0
94-95	33.4018992083121	35.0	33.0	35.0	29.0	36.0
96-97	33.5732728673545	35.0	33.5	35.0	30.0	36.0
98-99	33.668514154956426	35.0	34.0	35.0	30.0	36.0
100	32.87001744812075	34.0	32.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	6.0
15	20.0
16	46.0
17	249.0
18	1544.0
19	7550.0
20	23427.0
21	51732.0
22	86209.0
23	117300.0
24	145429.0
25	171058.0
26	195150.0
27	218272.0
28	245996.0
29	280342.0
30	322904.0
31	378603.0
32	454376.0
33	557444.0
34	717823.0
35	949130.0
36	1372335.0
37	2294480.0
38	3256553.0
39	2083255.0
40	111177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.67975164384129	22.264028107987702	14.593516470885989	23.462703777285014
2	13.94150781278489	23.175459468329503	41.56268945091711	21.320343267968493
3	16.319872067580405	29.736803088130003	32.080858746936244	21.862466097353348
4	11.306299619196663	20.293510770603746	40.868577571263906	27.531612038935688
5	11.229322445643893	40.00837620516588	36.83610399459785	11.926197354592377
6	26.192357313511323	41.57964768529082	19.942665323245713	12.285329677952143
7	23.4048317171491	34.594012583647185	25.62310760308783	16.378048096115887
8	23.294642513157875	37.39635726187976	22.60041393359473	16.708586291367634
9	25.50692085999973	16.003084958123047	22.28908592804755	36.200908253829674
10-11	22.684987164712567	27.793289843052417	30.555689679312238	18.966033312922782
12-13	24.045591761326076	25.821473393572063	30.4032026421684	19.729732202933466
14-15	21.431283875737396	26.711792597657674	28.19688705066329	23.66003647594164
16-17	20.198128370226645	29.739280657265567	28.881372900232833	21.18121807227496
18-19	20.234929742477984	28.7532425877579	30.98281342142742	20.0290142483367
20-21	21.53603188585005	27.750648524193156	30.622979531910726	20.09034005804607
22-23	21.138549380854187	27.81968794808249	30.47608781087131	20.56567486019202
24-25	20.731926329769443	28.07933236675149	30.65469316267218	20.53404814080689
26-27	20.654907926443652	28.218525053020908	30.562564349171378	20.564002671364065
28-29	20.603451419313178	27.945887805441398	30.781699587497492	20.66896118774793
30-31	20.983371649022462	28.033681412911584	30.43556392175005	20.547383016315912
32-33	20.756752477387362	28.201577169008285	30.34703901402486	20.694631339579495
34-35	20.73987150899337	28.330609774137894	30.4871459078092	20.442372809059535
36-37	20.94404132214473	28.156791621980293	30.13592362920432	20.763243426670652
38-39	20.696458282937513	28.25487417337046	30.27265105767712	20.776016486014907
40-41	20.89416173062812	28.116032743502412	30.232564624854252	20.75724090101521
42-43	20.949743024393914	28.24176500558445	29.81811188601885	20.990380084002787
44-45	20.911241587816683	28.54795279940599	29.566970835534505	20.97383477724282
46-47	21.238959939605213	28.44717985004621	29.04083825082398	21.273021959524595
48-49	21.186520685703655	28.539794267680467	28.979124424757757	21.294560621858118
50-51	21.105129098080734	28.6594401129367	28.805416908639504	21.43001388034306
52-53	21.188420081433264	28.641307502220155	28.873729838522543	21.296542577824038
54-55	21.23775478280963	28.590971574702024	28.847033790885835	21.32423985160251
56-57	21.36870622897625	28.686239544087684	28.628594580724016	21.316459646212056
58-59	21.104817321854462	28.77446556861164	28.893282089471512	21.227435020062384
60-61	21.32669241425918	29.014190854672012	28.535093965282616	21.124022765786194
62-63	21.14893377439666	29.11217363772769	28.60289479436524	21.135997793510413
64-65	21.0787979085805	28.920808547469907	28.666481827960023	21.333911715989572
66-67	21.296836491969977	28.964295372209538	28.456282161795443	21.28258597402504
68-69	21.214734682723055	28.924050364450878	28.58182933451477	21.2793856183113
70-71	21.289286434060312	28.531460574379253	28.72838639609353	21.450866595466902
72-73	21.360304973677678	28.65992761505322	28.590395277016604	21.389372134252497
74-75	21.15185946245076	28.830786558884007	28.59966168931904	21.417692289346192
76-77	21.242943854558547	28.705506777642903	28.67075683898098	21.38079252881757
78-79	21.2089772595829	28.709963989017584	28.584193522846206	21.49686522855331
80-81	21.179422081907862	28.823400463807396	28.50984256044263	21.487334893842107
82-83	21.374464462910968	28.632279424559226	28.64708399761095	21.34617211491885
84-85	21.344398674505268	28.671260186472324	28.525421867996897	21.458919271025508
86-87	21.158096218649213	28.865546562783035	28.665857847354875	21.310499371212877
88-89	21.341432954599114	28.667368893842777	28.599678894896062	21.391519256662047
90-91	21.33112511453042	28.71174916869767	28.651515110980185	21.305610605791724
92-93	21.383845006605604	28.776057765986845	28.517882384164906	21.322214843242644
94-95	21.11693158019495	28.724045620228306	28.697829979214706	21.461192820362037
96-97	21.212239413626353	28.873454778781472	28.78135871690244	21.13294709068974
98-99	22.073634937888823	30.812135145870467	29.02454375526557	18.08968616097514
100	21.311199866391263	31.714314738975258	22.780253599656273	24.194231794977206
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2751.0
1	2978.5
2	6224.0
3	9383.0
4	11919.0
5	20781.5
6	28402.0
7	32140.5
8	32818.5
9	29145.0
10	23873.0
11	18307.5
12	18426.0
13	26077.5
14	46998.0
15	78349.5
16	104329.0
17	117943.0
18	119880.5
19	112938.0
20	100826.5
21	88815.5
22	82405.5
23	86665.5
24	101911.0
25	127064.5
26	161512.5
27	198656.5
28	235495.0
29	277151.0
30	318965.5
31	357437.0
32	403103.5
33	450835.5
34	493376.5
35	531638.0
36	571268.0
37	603756.5
38	617038.0
39	623072.0
40	626122.5
41	619031.0
42	606358.5
43	606839.5
44	616715.5
45	618924.5
46	601010.0
47	577202.5
48	559465.0
49	535463.5
50	509419.0
51	479232.0
52	444373.5
53	406528.5
54	365867.5
55	328424.5
56	293625.5
57	258863.0
58	226046.0
59	197240.0
60	166792.0
61	133261.0
62	103116.0
63	80174.0
64	62145.5
65	47223.0
66	35580.0
67	27357.0
68	21461.5
69	16370.5
70	12339.5
71	9272.0
72	6939.0
73	5318.5
74	4013.0
75	2931.5
76	2138.5
77	1559.0
78	1165.0
79	864.0
80	643.5
81	481.5
82	370.0
83	263.5
84	195.0
85	154.5
86	118.0
87	90.5
88	59.0
89	33.5
90	23.5
91	19.0
92	13.5
93	8.5
94	6.5
95	5.0
96	4.0
97	3.5
98	2.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013088917617725841
3	0.0
4	0.12712913890731323
5	0.03582717330510267
6	0.004628833760349182
7	0.004970655330344198
8	4.628833760349182E-4
9	5.98187747491279E-4
10-11	0.00467156145659856
12-13	6.0530903019950846E-5
14-15	5.340962031172134E-5
16-17	3.809886248902788E-4
18-19	2.1363848124688534E-5
20-21	8.553304119998295E-4
22-23	0.03024227541809723
24-25	0.0010147862938675322
26-27	0.004473307576863323
28-29	5.880104455036042E-4
30-31	0.0027258436045433845
32-33	0.0034382145265286056
34-35	0.020089132595897025
36-37	0.004491716781262047
38-39	0.0016789120473205776
40-41	0.0010187900581558086
42-43	0.0010852955150782472
44-45	0.030855642938499916
46-47	0.0040408974259213745
48-49	0.005909974355392466
50-51	0.019965430336684378
52-53	0.004477350402820126
54-55	0.0010412029745601149
56-57	0.0013516063476142384
58-59	0.004260253515836744
60-61	0.0030092391101605343
62-63	8.294374905745739E-4
64-65	8.886468384167106E-5
66-67	0.0
68-69	2.9864773603591356E-4
70-71	0.014063525853462969
72-73	0.004230835821573325
74-75	0.0031918466608173387
76-77	0.0
78-79	0.0
80-81	9.28468123228889E-5
82-83	4.702850176457992E-6
84-85	1.9043913646135328E-5
86-87	0.0057693734185735365
88-89	0.0
90-91	2.47713615712653E-5
92-93	4.529037470840547E-5
94-95	3.427203762353599E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	8673.0
20-21	17281.0
22-23	18676.0
24-25	20364.0
26-27	24403.0
28-29	35352.0
30-31	57356.0
32-33	65389.0
34-35	79553.0
36-37	102925.0
38-39	132881.0
40-41	130612.0
42-43	147703.0
44-45	177227.0
46-47	169024.0
48-49	147271.0
50-51	127042.0
52-53	114610.0
54-55	116750.0
56-57	123579.0
58-59	129356.0
60-61	130434.0
62-63	119606.0
64-65	126084.0
66-67	134245.0
68-69	140166.0
70-71	140368.0
72-73	122495.0
74-75	119090.0
76-77	125049.0
78-79	133464.0
80-81	140136.0
82-83	131287.0
84-85	127977.0
86-87	131582.0
88-89	143495.0
90-91	155499.0
92-93	159913.0
94-95	173011.0
96-97	415273.0
98-99	820577.0
100-101	8406636.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.46330387964781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.23640838421638	74.47965470100203
2	7.197499270562657	12.014541375850289
3	1.9073081911595489	4.775707294526724
4	0.7419022310625493	2.476864454406489
5	0.3573462211084374	1.4912648121308663
6	0.1867574281861724	0.9352435188290403
7	0.1168011720953978	0.6824028204068059
8	0.06490716557810058	0.43338931876893
9	0.04610289175339079	0.3463109697729388
>10	0.13892594723048296	1.7801918540324024
>50	0.004112615719330773	0.2306882855544646
>100	0.0018321855239554847	0.28473482980839365
>500	6.420535070431333E-5	0.03713310159856824
>1k	3.2090452887147786E-5	0.03187266331201756
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.121282708229511E-6
2	0.0	7.121282708229511E-6	0.0	0.0	1.4242565416459021E-5
3	0.0	7.121282708229511E-6	0.0	0.0	1.4242565416459021E-5
4	0.0	4.272769624937707E-5	0.0	3.560641354114755E-5	2.1363848124688534E-5
5	7.121282708229511E-6	4.272769624937707E-5	0.0	4.272769624937707E-5	2.1363848124688534E-5
6	7.121282708229511E-6	4.272769624937707E-5	0.0	4.272769624937707E-5	2.1363848124688534E-5
7	1.4242565416459021E-5	4.9848978957606576E-5	0.0	4.9848978957606576E-5	2.1363848124688534E-5
8	1.4242565416459021E-5	4.9848978957606576E-5	0.0	5.6970261665836085E-5	2.1363848124688534E-5
9	2.1363848124688534E-5	1.2106180603990169E-4	0.0	1.1394052333167217E-4	5.6970261665836085E-5
10-11	2.1363848124688534E-5	1.2106180603990169E-4	0.0	1.6022886093516398E-4	5.6970261665836085E-5
12-13	2.1363848124688534E-5	1.2106180603990169E-4	0.0	2.0295655718454106E-4	5.6970261665836085E-5
14-15	3.560641354114756E-5	2.4212361207980336E-4	0.0	2.2788104666334434E-4	7.477346843640987E-5
16-17	4.272769624937707E-5	2.563661774962624E-4	0.0	2.6704810155860667E-4	7.833410979052463E-5
18-19	4.9848978957606576E-5	2.6704810155860667E-4	0.0	3.0621515645386894E-4	8.545539249875413E-5
20-21	5.6970261665836085E-5	2.777300256209509E-4	0.0	3.4894285270324605E-4	8.545539249875413E-5
22-23	5.6970261665836085E-5	2.812906669750657E-4	0.0	3.8810990759850837E-4	8.901603385286889E-5
24-25	6.053090301995084E-5	2.955332323915247E-4	0.0	4.2371632113965586E-4	9.257667520698364E-5
26-27	6.765218572818035E-5	2.990938737456395E-4	0.0	5.305355617630986E-4	9.257667520698364E-5
28-29	7.12128270822951E-5	2.990938737456395E-4	0.0	6.515973678030003E-4	9.61373165610984E-5
30-31	7.477346843640987E-5	3.987918316608526E-4	0.0	0.0012569063980025087	9.969795791521315E-5
32-33	7.833410979052463E-5	4.059131143690821E-4	0.0	0.002374947783194542	1.1394052333167217E-4
34-35	7.833410979052463E-5	4.700046587431477E-4	0.0	0.0038454926624439355	1.3886501281047545E-4
36-37	7.833410979052463E-5	5.447781271795576E-4	0.0	0.006017483888453936	1.4598629551870496E-4
38-39	7.833410979052463E-5	5.554600512419018E-4	0.0	0.009727672179441512	1.531075782269345E-4
40-41	7.833410979052463E-5	6.053090301995084E-4	0.0	0.014306656960833088	1.5666821958104925E-4
42-43	8.189475114463938E-5	6.26672878324197E-4	0.0	0.018419197724835627	1.5666821958104925E-4
44-45	8.545539249875413E-5	6.729612159276888E-4	0.0	0.02262431516404516	1.6378950228927876E-4
46-47	8.545539249875413E-5	6.978857054064921E-4	0.0	0.027384892654496583	1.7091078499750827E-4
48-49	8.545539249875413E-5	7.014463467606069E-4	0.0	0.03203865090432457	1.922746331221968E-4
50-51	8.545539249875413E-5	8.082655873840495E-4	0.0	0.03691316891810767	1.922746331221968E-4
52-53	8.545539249875413E-5	8.118262287381642E-4	0.0	0.041552684602519194	2.065171985386558E-4
54-55	8.545539249875413E-5	8.15386870092279E-4	0.0	0.045893106413185086	2.2075976395511483E-4
56-57	8.545539249875413E-5	8.189475114463938E-4	0.0	0.05018723988624747	2.2075976395511483E-4
58-59	8.545539249875413E-5	8.189475114463938E-4	0.0	0.05439591796681112	2.2432040530922959E-4
60-61	8.545539249875413E-5	8.225081528005086E-4	0.0	0.05895709954143212	2.2788104666334434E-4
62-63	8.545539249875413E-5	8.474326422793118E-4	0.0	0.06360017586719777	2.3500232937157385E-4
64-65	8.545539249875413E-5	0.001004100861860361	0.0	0.06745991109505817	2.3856297072568863E-4
66-67	8.545539249875413E-5	0.0010112221445685906	0.0	0.07136593466052205	2.4212361207980339E-4
68-69	8.545539249875413E-5	0.0010397072754015086	0.0	0.07555680953431511	2.4212361207980339E-4
70-71	8.545539249875413E-5	0.0010503891994638529	0.0	0.07936313514186379	2.4212361207980339E-4
72-73	8.545539249875413E-5	0.0010646317648803118	0.0	0.08243952927181894	2.563661774962624E-4
74-75	8.545539249875413E-5	0.001132283950608492	0.0	0.08471477909709826	2.563661774962624E-4
76-77	8.545539249875413E-5	0.0012212999844613613	0.0	0.08682623942008831	2.563661774962624E-4
78-79	8.545539249875413E-5	0.0012462244739401644	0.0	0.08828254173392125	2.563661774962624E-4
80-81	8.545539249875413E-5	0.0012462244739401644	0.0	0.0888237592197467	2.634874602044919E-4
82-83	8.545539249875413E-5	0.0012569063980025087	0.0	0.08914065630026291	2.777300256209509E-4
84-85	8.545539249875413E-5	0.0012853915288354267	0.0	0.08926883938901103	2.777300256209509E-4
86-87	8.545539249875413E-5	0.0013031947356060005	0.0	0.08933649157473922	2.884119496832952E-4
88	8.545539249875413E-5	0.0013672862799800661	0.0	0.08934361285744745	2.9197259103741E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25375	0.0	49.484947	1
GTATCAA	47115	0.0	37.348694	1
ATCAACG	61860	0.0	28.019968	3
TCAACGC	61865	0.0	28.011042	4
CAACGCA	62025	0.0	27.903255	5
TATCAAC	63470	0.0	27.51688	2
AACGCAG	64230	0.0	27.083271	6
CTTATAC	10450	0.0	25.120594	1
ACGCAGA	72820	0.0	23.76383	7
CGCAGAG	73455	0.0	23.547197	8
TATACAC	16470	0.0	21.168253	3
GCAGAGT	81420	0.0	20.814144	9
GTACATG	61685	0.0	20.256123	1
TACATGG	61630	0.0	19.86631	2
ACATGGG	60925	0.0	19.683653	3
AGAGTAC	77065	0.0	18.792381	10-11
TATGCCG	7195	0.0	18.463455	42-43
GAGTACT	46465	0.0	18.442945	12-13
CAGAGTA	81250	0.0	17.811768	10-11
CTCGTAT	7020	0.0	17.781986	38-39
>>END_MODULE