FastQCFastQC Report
Fri 27 May 2016
SRR2139663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27972172
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA546600.052.138151
GTATCAA1020250.038.045411
CTTATAC295850.036.884691
TATACAC364500.031.9404243
ATCAACG1247350.030.5999073
TCAACGC1251450.030.5198944
CAACGCA1254500.030.4255055
TATCAAC1295400.029.7297252
AACGCAG1302150.029.4223526
TTATACA427600.025.9345992
ACGCAGA1480650.025.7560527
CGCAGAG1498750.025.4337848
ACACATC441950.024.7478926
ACATCTC441750.024.6421818
GCAGAGT1692550.022.3245629
TACACAT533200.022.2860035
CACATCT484900.022.1446327
CATCTCC493900.021.6129559
GTACATG1324150.021.4457571
TACATGG1311750.021.2944892
Produced by FastQC (version 0.11.5)