##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139663_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27972172
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0712282192459	31.0	30.0	33.0	28.0	34.0
2	31.606593760398727	33.0	31.0	34.0	28.0	34.0
3	31.70677911604433	33.0	31.0	34.0	28.0	34.0
4	35.09696372523378	37.0	35.0	37.0	32.0	37.0
5	35.10739230403703	37.0	35.0	37.0	32.0	37.0
6	35.17994333797175	37.0	35.0	37.0	32.0	37.0
7	35.26500101600977	37.0	35.0	37.0	33.0	37.0
8	35.4593848128776	37.0	35.0	37.0	33.0	37.0
9	37.294162248108584	39.0	37.0	39.0	34.0	39.0
10-11	37.42968983602704	39.0	37.0	39.0	34.5	39.0
12-13	37.44883582869432	39.0	37.0	39.0	34.0	39.0
14-15	38.73173027107084	40.0	38.0	41.0	34.0	41.0
16-17	38.785405205573596	40.0	38.0	41.0	34.5	41.0
18-19	38.86362814442869	40.0	38.5	41.0	35.0	41.0
20-21	38.90328578596787	40.0	39.0	41.0	35.0	41.0
22-23	38.865935734009575	40.0	39.0	41.0	35.0	41.0
24-25	38.84012747994362	40.0	38.0	41.0	35.0	41.0
26-27	38.75192850969973	40.0	38.0	41.0	34.0	41.0
28-29	38.691903339333386	40.0	38.0	41.0	34.0	41.0
30-31	38.57809177939094	40.0	38.0	41.0	34.0	41.0
32-33	38.480813319657045	40.0	38.0	41.0	34.0	41.0
34-35	38.42794798753703	40.0	38.0	41.0	34.0	41.0
36-37	38.364135691400435	40.0	38.0	41.0	34.0	41.0
38-39	38.285962195264204	40.0	38.0	41.0	34.0	41.0
40-41	38.270011914812414	40.0	38.0	41.0	33.5	41.0
42-43	38.21675816740645	40.0	38.0	41.0	33.0	41.0
44-45	38.1655542757496	40.0	38.0	41.0	33.0	41.0
46-47	38.125345778690274	40.0	38.0	41.0	33.0	41.0
48-49	38.040824573769164	40.0	37.5	41.0	33.0	41.0
50-51	37.96010221037227	40.0	37.0	41.0	33.0	41.0
52-53	37.88148807983525	40.0	37.0	41.0	33.0	41.0
54-55	37.756240541934915	40.0	37.0	41.0	33.0	41.0
56-57	37.59535622027889	39.5	36.0	41.0	32.5	41.0
58-59	37.453759451063036	39.0	36.0	41.0	32.0	41.0
60-61	37.28217360144117	39.0	35.5	41.0	32.0	41.0
62-63	37.101846292788764	39.0	35.0	40.5	32.0	41.0
64-65	36.87948335492415	38.5	35.0	40.0	31.0	41.0
66-67	36.628831360810345	38.0	35.0	40.0	31.0	41.0
68-69	36.34787420264007	37.0	35.0	40.0	31.0	41.0
70-71	36.06260217811376	37.0	35.0	39.5	31.0	41.0
72-73	35.73814062868222	36.5	34.5	39.0	31.0	41.0
74-75	35.41294289881722	36.0	34.0	39.0	30.0	40.0
76-77	35.39051526256675	36.0	34.0	38.5	31.0	40.0
78-79	35.13476987176279	35.0	34.0	37.0	31.0	39.0
80-81	34.853161312725895	35.0	34.0	37.0	31.0	39.0
82-83	34.558940029438645	35.0	34.0	36.5	30.5	39.0
84-85	34.30904267734958	35.0	34.0	36.0	30.0	37.0
86-87	34.10860910121495	35.0	34.0	36.0	30.0	37.0
88-89	33.929997418717505	35.0	34.0	35.5	30.0	37.0
90-91	33.8044531418863	35.0	34.0	35.0	30.0	36.0
92-93	33.71793216065217	35.0	34.0	35.0	30.0	36.0
94-95	33.67097885070912	35.0	34.0	35.0	30.0	36.0
96-97	33.66925193758193	35.0	34.0	35.0	30.0	36.0
98-99	33.71054254393462	35.0	34.0	35.0	30.5	35.5
100	33.22468246383814	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	16.0
17	397.0
18	3821.0
19	13339.0
20	26711.0
21	42461.0
22	59878.0
23	81928.0
24	111763.0
25	147693.0
26	193049.0
27	245311.0
28	303386.0
29	371082.0
30	453738.0
31	557686.0
32	701413.0
33	914028.0
34	1270547.0
35	1839785.0
36	2945337.0
37	5455142.0
38	7805685.0
39	4249605.0
40	178370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.06074880420441	20.316488115402695	9.66452301237101	27.95824006802189
2	13.835922230480666	23.004747354169638	38.894694825459034	24.264635589890663
3	16.69023413698443	29.41138071080072	29.542178562322587	24.356206589892267
4	11.296502109310639	20.444172157957556	38.918876231706285	29.340449501025518
5	11.920554471065028	40.11184401411517	35.099330148549065	12.868271366270733
6	26.63249949982002	41.318041102137826	19.077447167193682	12.97201223084847
7	24.37124888152172	35.271345002103345	23.412048631072675	16.94535748530226
8	24.29296516552236	37.1677429982913	21.21321862313731	17.326073213049025
9	26.310298678272105	16.643820150970043	21.104896680886988	35.940984489870864
10-11	23.254804811010025	27.98110028781462	29.14757566913288	19.616519232042474
12-13	24.600606631476456	25.834452540903868	29.21995117147142	20.34498965614826
14-15	21.95651219158664	26.48076048958502	27.288235398948853	24.27449191987949
16-17	20.660102090472304	29.64973753087546	28.03045697183746	21.659703406814774
18-19	20.669394210789207	28.515168575397006	30.119816938062588	20.6956202757512
20-21	21.98026360872798	27.471712727116138	29.865108002439495	20.682915661716393
22-23	21.492132221636453	27.72734673134569	29.695969612907515	21.084551434110345
24-25	21.250485502144233	27.856886462008596	29.942290021744444	20.95033801410273
26-27	21.087846142961396	28.047577967926703	29.87193330350535	20.992642585606553
28-29	20.788351183609528	27.683388228056632	30.195468247343666	21.332792340990174
30-31	21.410079126348812	27.59744610973222	30.1426368073491	20.849837956569868
32-33	20.96759959088954	28.079747698393152	29.885162102989266	21.067490607728033
34-35	21.045882411815658	28.007742177442296	30.252423303925895	20.693952106816145
36-37	21.339758172403723	27.765147339499936	29.84022649896088	21.054867989135463
38-39	20.965291056141098	27.782029696231653	30.096389878834334	21.156289368792923
40-41	21.214677731993774	27.629419062959187	30.036474732284024	21.119428472763015
42-43	21.40543268550115	27.675111007610138	29.606700905067473	21.31275540182124
44-45	21.217167548271416	28.236600669552903	29.353236238633475	21.19299554354221
46-47	21.613813946565536	27.909129152320507	28.722141016197746	21.75491588491622
48-49	21.59111252030733	28.053082270007973	28.619625690395438	21.736179519289262
50-51	21.41114360798737	28.178346086318996	28.64904033672427	21.76146996896937
52-53	21.43329844672165	28.204045618072843	28.801676585992475	21.56097934921303
54-55	21.505956147738495	28.13752849340726	28.633491003926753	21.723024354927492
56-57	21.756775613745987	28.413418559904102	28.17639958286371	21.653406243486206
58-59	21.36199648929045	28.540566309516702	28.70762803214701	21.38980916904584
60-61	21.70644250572278	28.90837546286767	28.05074556285474	21.334436468554806
62-63	21.403263165658714	29.151643840922954	28.117572231596043	21.327520761822285
64-65	21.34668555072847	28.914431596656957	28.22463836623544	21.514244486379134
66-67	21.573376625687164	28.913703729662544	27.972857984656414	21.54006165999388
68-69	21.526837900041212	28.731306052535516	28.161049612874443	21.580806434548823
70-71	21.60514460877185	28.224158908012694	28.343377210909036	21.827319272306415
72-73	21.67895277515302	28.247733009630345	28.296456780071793	21.77685743514484
74-75	21.536162298904156	28.431881668322923	28.25410711712164	21.777848915651283
76-77	21.57053466686011	28.37282994161877	28.248948576958938	21.807686814562185
78-79	21.588205911338516	28.36099272855657	28.21973679756574	21.831064562539165
80-81	21.563774733192815	28.470426688199126	28.126016337401584	21.839782241206475
82-83	21.788959199878587	28.240902473598467	28.278316571070146	21.691821755452803
84-85	21.76260899756266	28.320629676834553	28.16440456563903	21.75235675996376
86-87	21.510848166317427	28.484257202968017	28.30720789461576	21.6976867360988
88-89	21.67129943711741	28.350939384490264	28.22282004323396	21.75494113515837
90-91	21.739437162278637	28.27927926012191	28.303754671598703	21.677528906000752
92-93	21.72724908466727	28.38526969214787	28.21730276113333	21.670178462051528
94-95	21.45210921549615	28.35566472340628	28.407083325922116	21.785142735175448
96-97	21.587959926834788	28.572717860544184	28.381343713998593	21.457978498622442
98-99	22.41382021125082	30.538099855111493	28.681023020455164	18.367056913182523
100	21.73109135449409	31.353575892212433	22.467644651895856	24.44768810139762
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38642.0
1	35056.5
2	22078.5
3	11940.0
4	11451.5
5	13203.5
6	16218.0
7	22092.0
8	32498.5
9	40245.5
10	40367.0
11	39687.5
12	47003.0
13	65917.0
14	108155.0
15	166613.5
16	215725.0
17	246296.5
18	251345.5
19	230986.5
20	196982.0
21	160722.0
22	135764.5
23	132863.5
24	150609.0
25	186886.0
26	243210.5
27	311795.0
28	376186.5
29	446507.5
30	524629.0
31	598074.5
32	683768.0
33	774520.0
34	857307.5
35	932865.5
36	1017613.0
37	1084911.0
38	1111321.5
39	1131370.5
40	1146501.5
41	1145978.5
42	1138908.0
43	1154798.5
44	1214368.5
45	1261646.0
46	1234240.0
47	1191253.5
48	1152405.5
49	1105147.5
50	1069471.5
51	1014564.5
52	940315.5
53	858358.0
54	771864.0
55	694096.5
56	625978.0
57	556229.0
58	487696.0
59	427536.5
60	362674.5
61	286639.5
62	215973.5
63	163987.5
64	122602.0
65	89945.0
66	66677.0
67	50351.5
68	38020.0
69	27726.5
70	20067.5
71	14538.0
72	10639.0
73	8292.5
74	5953.0
75	3991.0
76	2776.0
77	1743.5
78	1123.5
79	709.0
80	448.0
81	289.0
82	210.5
83	159.0
84	120.5
85	93.0
86	74.5
87	60.0
88	37.5
89	25.0
90	23.5
91	19.0
92	13.5
93	7.5
94	5.0
95	7.0
96	7.5
97	7.0
98	11.0
99	15.0
100	22.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002867135237120664
3	0.0
4	0.0
5	0.0
6	0.00800795876702031
7	5.362472388629671E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.1976188334606265E-4
16-17	4.289977910903737E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.4107069588691895E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	1.8079230383072314E-6
36-37	9.349741118376819E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.00389810538336289
52-53	0.0
54-55	0.0
56-57	8.869769607734438E-4
58-59	0.0032816008782873395
60-61	0.003606944836964583
62-63	0.00237457471955846
64-65	0.0027449789776604395
66-67	0.0028630521874008327
68-69	0.0014250983855954734
70-71	0.0
72-73	0.0038853614596194427
74-75	0.0031623279369399067
76-77	0.006076165747808621
78-79	1.7538874916251875E-5
80-81	1.94879514190174E-4
82-83	0.003278689698630004
84-85	0.001996151068079851
86-87	8.448853903834049E-5
88-89	3.9240211633079276E-5
90-91	4.1828149026357524E-4
92-93	0.0
94-95	4.8072508726362145E-4
96-97	5.688110877364623E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	7827.0
20-21	19934.0
22-23	25594.0
24-25	25178.0
26-27	29408.0
28-29	41903.0
30-31	66241.0
32-33	76528.0
34-35	103464.0
36-37	158393.0
38-39	218067.0
40-41	219702.0
42-43	250934.0
44-45	305694.0
46-47	289954.0
48-49	245391.0
50-51	208510.0
52-53	185085.0
54-55	190055.0
56-57	203252.0
58-59	217348.0
60-61	217524.0
62-63	202308.0
64-65	237089.0
66-67	238803.0
68-69	258291.0
70-71	270157.0
72-73	201115.0
74-75	192053.0
76-77	204825.0
78-79	224355.0
80-81	238726.0
82-83	226236.0
84-85	219632.0
86-87	229175.0
88-89	255882.0
90-91	284450.0
92-93	299052.0
94-95	350445.0
96-97	877421.0
98-99	1707979.0
100-101	1.7948192E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.57547091500747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.2557655649025	66.05075867266895
2	8.525801691127374	13.057345588520915
3	2.39758113557842	5.5078771354156775
4	1.086289093621252	3.3273239557553604
5	0.5753989622969289	2.203072325094698
6	0.33622279891797363	1.5447851495703415
7	0.2150488381577786	1.1527226236159986
8	0.14332173537617868	0.8779943503029575
9	0.09723766931264101	0.6701418286463932
>10	0.3550451836590328	4.182135012165896
>50	0.008092995328003791	0.41906446522625673
>100	0.003626209108231406	0.5398738413484447
>500	4.0601030012855894E-4	0.20844938218666162
>1k	1.6211231364915364E-4	0.25845566948135457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.149963184839561E-6	0.0
2	0.0	4.289977910903737E-5	0.0	7.149963184839561E-6	0.0
3	0.0	4.289977910903737E-5	0.0	3.2174834331778023E-5	0.0
4	0.0	1.0367446618017364E-4	0.0	3.932479751661759E-5	0.0
5	0.0	1.108244293650132E-4	0.0	4.289977910903737E-5	0.0
6	0.0	1.1439941095743297E-4	0.0	6.792465025597584E-5	3.5749815924197804E-6
7	0.0	1.1439941095743297E-4	0.0	6.792465025597584E-5	3.5749815924197804E-6
8	0.0	1.3942428210437144E-4	0.0	8.222457662565496E-5	7.149963184839561E-6
9	0.0	2.9314849057842203E-4	0.0	1.0367446618017364E-4	7.149963184839561E-6
10-11	3.5749815924197804E-6	3.0029845376326156E-4	0.0	1.3406180971574177E-4	8.937453981049452E-6
12-13	5.362472388629671E-6	3.0744841694810114E-4	0.0	1.5014922688163078E-4	1.0724944777259342E-5
14-15	1.2512435573469232E-5	3.9146048436996595E-4	0.0	1.5372420847405057E-4	3.0387343535568135E-5
16-17	1.7874907962098903E-5	4.003979383510154E-4	0.0	1.7517409802856926E-4	4.826225149766704E-5
18-19	1.9662398758308792E-5	4.200603371093243E-4	0.0	2.413112574883352E-4	5.004974229387693E-5
20-21	2.3237380350728575E-5	4.432977174600528E-4	0.0	2.6812361943148353E-4	5.54122146825066E-5
22-23	2.8599852739358243E-5	4.790475333842506E-4	0.0	2.9136099978221215E-4	7.32871226446055E-5
24-25	3.0387343535568135E-5	5.237348032894979E-4	0.0	3.449857236685088E-4	8.579955821807473E-5
26-27	3.2174834331778023E-5	5.469721836402265E-4	0.0	4.039729199434352E-4	8.579955821807473E-5
28-29	3.2174834331778023E-5	5.52334656028856E-4	0.0	7.239337724650055E-4	8.579955821807473E-5
30-31	4.289977910903737E-5	6.131093430999923E-4	0.0	0.0017964282501909397	8.579955821807473E-5
32-33	4.289977910903737E-5	6.327717418583012E-4	0.0	0.003769818089206659	9.831199379154397E-5
34-35	4.289977910903737E-5	7.275087540574254E-4	0.0	0.0063366548725640615	1.0367446618017364E-4
36-37	4.289977910903737E-5	8.007958767020308E-4	0.0	0.01007966059982757	1.0367446618017364E-4
38-39	4.289977910903737E-5	8.436956558110682E-4	0.0	0.01697222511001291	1.0724944777259342E-4
40-41	4.647476070145715E-5	9.420076496026122E-4	0.0	0.0247960723250236	1.0724944777259342E-4
42-43	4.647476070145715E-5	9.831199379154395E-4	0.0	0.03117026450430807	1.0903693856880332E-4
44-45	4.826225149766704E-5	0.0011028818212615024	0.0	0.03796630451149807	1.1261192016122308E-4
46-47	5.004974229387693E-5	0.0011976188334606266	0.0	0.04550951567150381	1.1618690175364287E-4
48-49	5.004974229387693E-5	0.0012011938150530465	0.0	0.052865040297907506	1.2512435573469233E-4
50-51	5.004974229387693E-5	0.0013996052934323441	0.0	0.06017766514520216	1.2512435573469233E-4
52-53	5.004974229387693E-5	0.0014442925633375914	0.0	0.06826784848884813	1.3406180971574177E-4
54-55	5.004974229387693E-5	0.001449655035726221	0.0	0.0760148335996218	1.447867544930011E-4
56-57	5.004974229387693E-5	0.0014693174344845299	0.0	0.08353301988848058	1.4657424528921102E-4
58-59	5.362472388629671E-5	0.0014728924160769498	0.0	0.09082240735542452	1.5551169927026045E-4
60-61	5.362472388629671E-5	0.0014818298700579992	0.0	0.09748259806210258	1.6444915325130991E-4
62-63	5.362472388629671E-5	0.001499704778020098	0.0	0.10368340363415468	1.823240612134088E-4
64-65	5.362472388629671E-5	0.0015336671031480858	0.0	0.10852392871029107	1.823240612134088E-4
66-67	5.362472388629671E-5	0.0015354545939442958	0.0	0.11321966703193445	1.8411155200961872E-4
68-69	5.362472388629671E-5	0.0015747793914609133	0.0	0.11791540535357783	1.858990428058286E-4
70-71	5.362472388629671E-5	0.0016015917534040617	0.0	0.1222464955527944	1.858990428058286E-4
72-73	5.362472388629671E-5	0.0016015917534040617	0.0	0.12583577707158386	1.9662398758308794E-4
74-75	5.362472388629671E-5	0.0016659414220676178	0.0	0.1285760004621736	1.9662398758308794E-4
76-77	5.362472388629671E-5	0.0017517409802856924	0.0	0.13113389979154996	1.9662398758308794E-4
78-79	5.362472388629671E-5	0.0017874907962098904	0.0	0.13264254202355114	1.9662398758308794E-4
80-81	5.362472388629671E-5	0.0017874907962098904	0.0	0.13320917660594966	2.1271140474897693E-4
82-83	5.362472388629671E-5	0.0017892782870061004	0.0	0.13379904856869893	2.377362758959154E-4
84-85	5.362472388629671E-5	0.00179464075939473	0.0	0.1340278473906138	2.395237666921253E-4
86-87	5.362472388629671E-5	0.001842903010892397	0.0	0.13417442163590299	2.4309874828454508E-4
88	5.362472388629671E-5	0.0019769648206081386	0.0	0.13418335908988405	2.4309874828454508E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	54660	0.0	52.13815	1
GTATCAA	102025	0.0	38.04541	1
CTTATAC	29585	0.0	36.88469	1
TATACAC	36450	0.0	31.940424	3
ATCAACG	124735	0.0	30.599907	3
TCAACGC	125145	0.0	30.519894	4
CAACGCA	125450	0.0	30.425505	5
TATCAAC	129540	0.0	29.729725	2
AACGCAG	130215	0.0	29.422352	6
TTATACA	42760	0.0	25.934599	2
ACGCAGA	148065	0.0	25.756052	7
CGCAGAG	149875	0.0	25.433784	8
ACACATC	44195	0.0	24.747892	6
ACATCTC	44175	0.0	24.642181	8
GCAGAGT	169255	0.0	22.324562	9
TACACAT	53320	0.0	22.286003	5
CACATCT	48490	0.0	22.144632	7
CATCTCC	49390	0.0	21.612955	9
GTACATG	132415	0.0	21.445757	1
TACATGG	131175	0.0	21.294489	2
>>END_MODULE