FastQCFastQC Report
Fri 27 May 2016
SRR2139662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15044007
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA271950.053.4514121
GTATCAA526350.039.8377721
TCAACGC659800.031.0923584
ATCAACG660000.031.0829333
CAACGCA663250.030.8863125
TATCAAC675950.030.5923462
AACGCAG683350.030.0761346
ACGCAGA778350.026.359997
CGCAGAG791450.025.9130738
GCAGAGT888950.022.9670269
GTACATG670300.020.0698641
TACATGG658100.020.0272142
ACATGGG650050.019.8679053
GAGTACT518100.019.82907912-13
CAGAGTA885500.019.34897810-11
AGAGTAC843400.019.05541210-11
GTACTTT554500.018.55379514-15
AGTACTT563850.017.91861312-13
CATGGGG374800.017.576644
ACTTTTT614700.016.88037916-17
Produced by FastQC (version 0.11.5)