FastQCFastQC Report
Fri 27 May 2016
SRR2139661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10340040
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110900.047.733581
GTATCAA198500.036.9449161
TCAACGC247250.028.971674
ATCAACG249650.028.7437883
CAACGCA253300.028.2963265
TATCAAC262650.027.648912
AACGCAG262150.027.4214296
ACGCAGA300750.023.8879937
CGCAGAG310950.023.07738
GTACATG302750.021.2579231
TACATGG300800.020.849482
ACATGGG305850.020.017883
GCAGAGT369650.019.3336989
AGAGTAC327700.018.56814410-11
GAGTACT196900.017.96605312-13
CATGGGG217550.017.8386274
CAGAGTA358400.017.09519810-11
GTGGTAT59350.016.6883371
AGTACTT221950.015.99530212-13
GTACTTT223350.015.92372614-15
Produced by FastQC (version 0.11.5)