##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139661_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10340040
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93691484752477	34.0	31.0	34.0	31.0	34.0
2	33.04343774298746	34.0	33.0	34.0	31.0	34.0
3	33.10753469038804	34.0	33.0	34.0	31.0	34.0
4	36.47307969795088	37.0	37.0	37.0	35.0	37.0
5	36.433707219701276	37.0	37.0	37.0	35.0	37.0
6	36.46307316025857	37.0	37.0	37.0	35.0	37.0
7	36.41826898155133	37.0	37.0	37.0	35.0	37.0
8	36.403364493754374	37.0	37.0	37.0	35.0	37.0
9	38.194235225395644	39.0	39.0	39.0	37.0	39.0
10-11	38.145831205681986	39.0	39.0	39.0	37.0	39.0
12-13	38.12013362617553	39.0	39.0	39.0	37.0	39.0
14-15	39.59674788492114	41.0	40.0	41.0	37.0	41.0
16-17	39.575420936476064	41.0	40.0	41.0	37.0	41.0
18-19	39.57256393592288	41.0	40.0	41.0	37.0	41.0
20-21	39.545059171163714	41.0	40.0	41.0	37.0	41.0
22-23	39.467274865013	41.0	39.0	41.0	36.5	41.0
24-25	39.31718104695067	41.0	39.0	41.0	36.0	41.0
26-27	39.323991216088544	40.5	39.0	41.0	36.0	41.0
28-29	39.24871127788731	41.0	39.0	41.0	36.0	41.0
30-31	39.175393543570785	41.0	39.0	41.0	36.0	41.0
32-33	38.994733336631725	40.0	39.0	41.0	35.0	41.0
34-35	38.920499721092156	40.0	38.5	41.0	35.0	41.0
36-37	39.12249172772593	40.5	39.0	41.0	35.0	41.0
38-39	39.19932137094803	41.0	39.0	41.0	35.0	41.0
40-41	39.18071816824755	41.0	39.0	41.0	35.0	41.0
42-43	39.03770602639371	41.0	39.0	41.0	35.0	41.0
44-45	39.061744147574124	41.0	39.0	41.0	35.0	41.0
46-47	39.01623978333848	40.0	39.0	41.0	35.0	41.0
48-49	38.95329957388609	40.0	39.0	41.0	35.0	41.0
50-51	38.878587540234406	40.0	38.5	41.0	35.0	41.0
52-53	38.76050046217673	40.0	38.0	41.0	35.0	41.0
54-55	38.622235420275175	40.0	38.0	41.0	35.0	41.0
56-57	38.47200719814536	40.0	37.5	41.0	34.5	41.0
58-59	38.30565419650628	40.0	37.0	41.0	34.0	41.0
60-61	38.11592058706884	40.0	37.0	41.0	34.0	41.0
62-63	37.88069517213734	39.0	36.0	41.0	34.0	41.0
64-65	37.60207723770263	39.0	36.0	41.0	33.5	41.0
66-67	37.27418455872533	38.5	35.0	40.0	33.0	41.0
68-69	36.934692613369336	37.5	35.0	40.0	33.0	41.0
70-71	36.570628476205115	37.0	35.0	40.0	32.5	41.0
72-73	36.22443869023833	37.0	35.0	39.0	32.0	41.0
74-75	35.880735439592655	36.0	35.0	39.0	32.0	40.5
76-77	35.01699478363901	35.0	34.0	37.0	30.5	39.0
78-79	35.22892527447091	35.0	34.0	37.0	32.0	39.0
80-81	35.01217014704564	35.0	34.0	37.0	32.0	39.0
82-83	34.751027517437265	35.0	34.0	36.5	31.5	38.5
84-85	34.49694042863832	35.0	34.0	36.0	31.0	37.0
86-87	34.28718724376729	35.0	34.0	36.0	31.0	37.0
88-89	34.09753407973897	35.0	34.0	35.0	31.0	36.5
90-91	33.90850857279378	35.0	34.0	35.0	31.0	36.0
92-93	33.780871331979455	35.0	34.0	35.0	31.0	36.0
94-95	33.712123148593804	35.0	34.0	35.0	31.0	36.0
96-97	33.68767823869218	35.0	34.0	35.0	31.0	35.5
98-99	33.708566612159295	35.0	34.0	35.0	31.0	35.0
100-101	32.32328923142491	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	37.0
21	175.0
22	883.0
23	2672.0
24	7028.0
25	14754.0
26	26321.0
27	41893.0
28	61393.0
29	83164.0
30	109814.0
31	140216.0
32	180150.0
33	240835.0
34	349826.0
35	530122.0
36	969188.0
37	2214996.0
38	3352901.0
39	1889296.0
40	124371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.04986441058255	20.792869273232988	14.375108800352804	24.78215751583166
2	13.863878873596422	21.64402141052939	41.33038017910471	23.16171953676948
3	17.044044317043262	26.525883845710464	31.052442737165425	25.37762910008085
4	11.845350694968298	18.552442737165425	39.203755498044494	30.398451069821782
5	12.971303786058854	38.084378783834495	35.8158962634574	13.12842116664926
6	29.690726534907018	38.37698887044924	18.988475866631077	12.943808728012657
7	26.483972823679153	32.76093659680517	23.315152536776726	17.43993804273894
8	24.479069753047497	36.86664673107179	21.15047982758934	17.503803688291374
9	25.60939189437504	16.67888139048625	21.132642422906038	36.57908429223267
10-11	23.932861961849277	26.78063624512091	29.37457688751688	19.911924905512937
12-13	24.929429673386178	24.35327619622361	29.26538001787227	21.45191411251794
14-15	22.05765173462465	25.836257700187964	26.68780334714882	25.41828721803857
16-17	21.721648994498036	28.985512572516075	27.285339328413265	22.00749910457262
18-19	21.748334629266424	28.242115117543065	28.890434659827235	21.119115593363276
20-21	22.578474249919704	27.360054500123937	28.691053099418916	21.370418150537443
22-23	22.30492558800773	27.283797492755195	28.498682918593783	21.912594000643296
24-25	22.115973075545522	27.43198813439591	28.54791211656075	21.90412667349782
26-27	22.04865497995121	27.567861664758386	28.505954151163408	21.877529204126994
28-29	21.985697124472548	27.6052160434668	28.550336554101886	21.858750277958766
30-31	22.23417319512673	27.50287460367523	28.411607262080896	21.851344939117144
32-33	22.083489668929356	27.64433158213086	28.332739684715293	21.93943906422449
34-35	22.1347600143742	27.652811015409647	28.39733505531784	21.815093914898316
36-37	22.14095223895311	27.56123429645391	28.362539699511085	21.93527376508189
38-39	22.000996451868797	27.676322247081934	28.42190293661912	21.900778364430145
40-41	22.218894336505134	27.583531244410626	28.255462880571997	21.94211153851225
42-43	22.196088364149773	27.6718318583405	28.05316995980028	22.078909817709444
44-45	22.22527619318215	27.744936194544533	27.938222675000006	22.09156493727331
46-47	22.289026812227352	27.681496708681745	27.711510892829033	22.31796558626187
48-49	22.237362897399375	27.762608636199026	27.707156085886343	22.292872380515256
50-51	22.214272132848468	27.85193718706976	27.666469557876656	22.267321122205118
52-53	22.43403324745003	27.763043724538004	27.538494010636366	22.2644290173756
54-55	22.385255577659066	27.72173655886335	27.524300644230948	22.368707219246637
56-57	22.37624671560411	27.820112739348534	27.499173121480393	22.30446742356696
58-59	22.31586920714416	27.847769054531714	27.50962423054224	22.32673750778189
60-61	22.41092206009148	27.869329217044104	27.478612070413476	22.241136652450937
62-63	22.417561149504944	27.92688042029775	27.41788038992557	22.237678040271735
64-65	22.354889971744676	27.826338701553944	27.435412161143812	22.38335916555757
66-67	22.435297209775225	27.862282498660797	27.306233541580916	22.39618674998306
68-69	22.3479759266518	27.830048079593155	27.408603025990143	22.413372967764904
70-71	22.328420991912623	27.67761661219696	27.468197173729465	22.525765222160953
72-73	22.423338988229432	27.689320668099608	27.399836918317256	22.4875034253537
74-75	22.292915227148136	27.833857925848765	27.34189887195192	22.53132797505118
76-77	22.403478713202787	27.734356663661803	27.29936782892008	22.562796794215334
78-79	22.34597155393377	27.757613450914008	27.270721831483918	22.625693163668306
80-81	22.380527363864978	27.787218611445702	27.263267672222756	22.568986352466567
82-83	22.420395149630526	27.763044300329216	27.318089419034745	22.498471131005513
84-85	22.4794492550514	27.7304577631041	27.29550833097046	22.494584650874042
86-87	22.36903508334951	27.72510875928054	27.35923451687683	22.546621640493118
88-89	22.417329323854673	27.698495594488293	27.34715581824949	22.537019263407547
90-91	22.431034086984432	27.768055551649805	27.340002201316327	22.46090816004943
92-93	22.492336828246838	27.685210300906704	27.35609919696519	22.466353673881272
94-95	22.284735845122714	27.75297147433192	27.409397915524274	22.552894765021094
96-97	22.328207372252855	27.793930070095374	27.41080578964736	22.467056768004415
98-99	22.510419529027427	28.280788784818633	27.46310921575495	21.745682470398993
100-101	20.39957389534159	32.039151994331874	25.48184157304051	22.079432537286024
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2636.0
1	2275.5
2	2681.5
3	3619.0
4	4719.0
5	7056.0
6	8921.0
7	10237.5
8	10969.0
9	10717.5
10	10034.0
11	9554.0
12	10800.5
13	14535.5
14	23227.5
15	35154.0
16	44820.5
17	50028.0
18	50694.0
19	48196.5
20	44313.5
21	40517.5
22	39255.0
23	42730.5
24	51192.0
25	64759.0
26	83233.5
27	104204.5
28	127577.5
29	153152.0
30	177053.5
31	200703.5
32	226406.5
33	252329.0
34	277397.0
35	301569.5
36	327293.5
37	349868.0
38	368061.0
39	385389.0
40	401504.0
41	415899.0
42	425538.5
43	433906.0
44	441522.0
45	446812.0
46	450172.0
47	449787.5
48	445886.0
49	439330.0
50	431476.0
51	419214.0
52	400301.0
53	378227.0
54	355285.5
55	328851.0
56	298874.0
57	268357.5
58	237582.0
59	204491.0
60	170024.5
61	137745.0
62	109172.5
63	84571.5
64	62753.5
65	46076.0
66	34822.5
67	26526.0
68	20076.5
69	14533.0
70	10218.5
71	7271.5
72	5122.0
73	3570.0
74	2372.5
75	1525.5
76	1019.5
77	665.5
78	436.0
79	290.5
80	198.0
81	129.5
82	80.5
83	52.5
84	36.5
85	26.0
86	22.5
87	20.5
88	12.0
89	3.5
90	3.0
91	4.0
92	3.5
93	2.0
94	1.0
95	1.0
96	1.0
97	1.5
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.029690407387205464
3	0.0
4	0.0
5	0.0
6	0.0
7	8.704028224262189E-5
8	7.350068278265848E-4
9	5.802685482841458E-4
10-11	0.0
12-13	0.0
14-15	0.0018858727819234741
16-17	5.319128359271337E-5
18-19	0.0
20-21	2.176054301936045E-4
22-23	0.001842511409788175
24-25	5.90084443502244E-4
26-27	0.014272557268756996
28-29	0.31263037561441437
30-31	0.3137866101748648
32-33	0.33098811131254546
34-35	0.2136064806921706
36-37	0.07214333586962247
38-39	0.06380310638752161
40-41	0.02554632756370653
42-43	0.010085275492765507
44-45	0.013095414068029613
46-47	0.009524153817337176
48-49	0.006552018759268117
50-51	0.007076182156189005
52-53	0.002879570962912708
54-55	0.001123082795207236
56-57	0.003435352551846002
58-59	0.0038833249321355025
60-61	0.003138588490060743
62-63	0.0022556930616932053
64-65	7.502150149824657E-4
66-67	0.007822612137739332
68-69	0.011896242779530218
70-71	0.01240957312574042
72-73	0.010605099700234648
74-75	0.010981872983005863
76-77	0.010261308962265563
78-79	0.002877026092627884
80-81	0.0022625603832299025
82-83	0.0019428611367624644
84-85	1.2565706866561931E-5
86-87	0.002996480566773802
88-89	0.0
90-91	0.00921381143140386
92-93	0.019387347860591892
94-95	0.0015441421349794652
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	137.0
20-21	506.0
22-23	1237.0
24-25	2745.0
26-27	5009.0
28-29	9997.0
30-31	24793.0
32-33	32624.0
34-35	45525.0
36-37	67384.0
38-39	78273.0
40-41	80258.0
42-43	91652.0
44-45	108053.0
46-47	106110.0
48-49	96236.0
50-51	87785.0
52-53	85500.0
54-55	92140.0
56-57	94616.0
58-59	93824.0
60-61	91614.0
62-63	90022.0
64-65	95585.0
66-67	99774.0
68-69	99563.0
70-71	97407.0
72-73	92590.0
74-75	93892.0
76-77	95330.0
78-79	100079.0
80-81	100461.0
82-83	96906.0
84-85	98004.0
86-87	102297.0
88-89	108049.0
90-91	112517.0
92-93	115602.0
94-95	129842.0
96-97	200697.0
98-99	867006.0
100-101	6248399.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.80558774380101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.04299053868789	77.26191725381626
2	7.049862376196618	12.098351694049207
3	1.6615837660015418	4.277195148819619
4	0.5926463256585909	2.0340946518935796
5	0.27653658774119816	1.1864192221899355
6	0.12885778962225797	0.6634031024342945
7	0.07107904352748302	0.4269285374300028
8	0.048150010862439384	0.33052319855376117
9	0.027930245429792902	0.21569140124387928
>10	0.09756992984439665	1.294775733392208
>50	0.002262451508363623	0.13021991992058762
>100	5.13007603364491E-4	0.06923542721758727
>500	1.7927316051141417E-5	0.011244709039094962
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	9.671142471402432E-6	1.9342284942804864E-5	0.0	9.671142471402432E-6	0.0
3	9.671142471402432E-6	1.9342284942804864E-5	0.0	9.671142471402432E-6	0.0
4	9.671142471402432E-6	6.769799729981703E-5	0.0	9.671142471402432E-6	0.0
5	1.9342284942804864E-5	6.769799729981703E-5	0.0	1.9342284942804864E-5	0.0
6	1.9342284942804864E-5	6.769799729981703E-5	0.0	2.9013427414207297E-5	0.0
7	3.868456988560973E-5	6.769799729981703E-5	0.0	3.868456988560973E-5	0.0
8	3.868456988560973E-5	7.736913977121946E-5	0.0	4.835571235701216E-5	0.0
9	3.868456988560973E-5	1.9342284942804863E-4	0.0	7.736913977121946E-5	0.0
10-11	4.835571235701216E-5	1.9342284942804863E-4	0.0	9.18758534783231E-5	0.0
12-13	7.253356853551824E-5	1.9342284942804863E-4	0.0	1.0638256718542674E-4	0.0
14-15	8.704028224262189E-5	2.514497042564632E-4	0.0	1.78916135720945E-4	4.835571235701216E-6
16-17	9.671142471402431E-5	2.6112084672786566E-4	0.0	1.837517069566462E-4	9.671142471402432E-6
18-19	9.671142471402431E-5	2.9496984537777415E-4	0.0	2.0309399189945106E-4	9.671142471402432E-6
20-21	9.671142471402431E-5	3.384899864990851E-4	0.0	2.2243627684225592E-4	1.4506713707103648E-5
22-23	9.671142471402431E-5	3.8201012762039603E-4	0.0	2.514497042564632E-4	3.3848998649908514E-5
24-25	1.2572485212823163E-4	4.25530268741707E-4	0.0	2.901342741420729E-4	5.802685482841459E-5
26-27	1.3539599459963406E-4	4.3520141121310943E-4	0.0	3.7233898514899363E-4	6.769799729981703E-5
28-29	1.3539599459963406E-4	4.3520141121310943E-4	0.0	8.075403963621031E-4	6.769799729981703E-5
30-31	1.5473827954243891E-4	5.028994085129264E-4	0.0	0.001924557351809084	7.253356853551824E-5
32-33	1.6440942201384134E-4	5.125705509843289E-4	0.0	0.004487410106730728	8.704028224262189E-5
34-35	1.837517069566462E-4	5.802685482841458E-4	0.0	0.008104417391035238	8.704028224262189E-5
36-37	2.0309399189945106E-4	6.479665455839629E-4	0.0	0.012823934917079624	8.704028224262189E-5
38-39	2.0309399189945106E-4	6.624732592910666E-4	0.0	0.020144989767931266	9.18758534783231E-5
40-41	2.127651343708535E-4	6.963222579409751E-4	0.0	0.027214594914526442	9.671142471402431E-5
42-43	2.127651343708535E-4	7.108289716480788E-4	0.0	0.03449212962425677	1.1121813842112796E-4
44-45	2.1760070560655472E-4	8.075403963621031E-4	0.0	0.0421710167465503	1.257248521282316E-4
46-47	2.6112084672786566E-4	8.800739648976213E-4	0.0	0.0509137295406981	1.257248521282316E-4
48-49	2.707919891992681E-4	8.94580678604725E-4	0.0	0.05964677119237449	1.4506713707103649E-4
50-51	2.707919891992681E-4	9.912921033187493E-4	0.0	0.06751908116409608	1.4506713707103649E-4
52-53	2.707919891992681E-4	0.0010057988170258528	0.0	0.07534787099469635	1.6440942201384134E-4
54-55	2.804631316706705E-4	0.0010057988170258528	0.0	0.08322501653765363	1.885872781923474E-4
56-57	2.998054166134754E-4	0.0010057988170258528	0.0	0.09118920236285352	1.9342284942804863E-4
58-59	3.1914770155628023E-4	0.001010634388261554	0.0	0.09869400892066182	1.9342284942804863E-4
60-61	3.1914770155628023E-4	0.0010348122444400602	0.0	0.10641158061284095	1.9342284942804863E-4
62-63	3.1914770155628023E-4	0.00107349681432567	0.0	0.1133264474798937	2.127651343708535E-4
64-65	3.1914770155628023E-4	0.0011798793815110966	0.0	0.12033802577166046	2.127651343708535E-4
66-67	3.2398327279198146E-4	0.001189550523982499	0.0	0.12735927520589863	2.127651343708535E-4
68-69	3.4816112897048754E-4	0.0012475773788109138	0.0	0.13487858847741402	2.127651343708535E-4
70-71	3.4816112897048754E-4	0.0012669196637537185	0.0	0.14185631777053087	2.2243627684225592E-4
72-73	3.4816112897048754E-4	0.0012717552349894197	0.0	0.14665320443634647	2.6112084672786566E-4
74-75	3.4816112897048754E-4	0.0013152753761107307	0.0	0.15058452385097154	2.6112084672786566E-4
76-77	3.5783227144188995E-4	0.0013346176610535354	0.0	0.15429340698875438	2.6112084672786566E-4
78-79	3.5783227144188995E-4	0.001344288803524938	0.0	0.15737850143713178	2.6112084672786566E-4
80-81	3.5783227144188995E-4	0.001344288803524938	0.0	0.15857288753235	2.8529870290637174E-4
82-83	3.626678426775912E-4	0.001344288803524938	0.0	0.1590709513696272	3.0947655908487783E-4
84-85	3.675034139132924E-4	0.0013539599459963404	0.0	0.15938042792871207	3.1914770155628023E-4
86-87	3.675034139132924E-4	0.0013733022309391453	0.0	0.1595206594945474	3.1914770155628023E-4
88-89	3.675034139132924E-4	0.0014216579432961575	0.0	0.15961253534802572	3.1914770155628023E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11090	0.0	47.73358	1
GTATCAA	19850	0.0	36.944916	1
TCAACGC	24725	0.0	28.97167	4
ATCAACG	24965	0.0	28.743788	3
CAACGCA	25330	0.0	28.296326	5
TATCAAC	26265	0.0	27.64891	2
AACGCAG	26215	0.0	27.421429	6
ACGCAGA	30075	0.0	23.887993	7
CGCAGAG	31095	0.0	23.0773	8
GTACATG	30275	0.0	21.257923	1
TACATGG	30080	0.0	20.84948	2
ACATGGG	30585	0.0	20.01788	3
GCAGAGT	36965	0.0	19.333698	9
AGAGTAC	32770	0.0	18.568144	10-11
GAGTACT	19690	0.0	17.966053	12-13
CATGGGG	21755	0.0	17.838627	4
CAGAGTA	35840	0.0	17.095198	10-11
GTGGTAT	5935	0.0	16.688337	1
AGTACTT	22195	0.0	15.995302	12-13
GTACTTT	22335	0.0	15.923726	14-15
>>END_MODULE