FastQCFastQC Report
Fri 27 May 2016
SRR2139660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10467634
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104800.044.7786181
GTATCAA180700.034.0725981
TCAACGC209850.028.1235544
ATCAACG215200.027.4833623
CAACGCA215750.027.3544755
AACGCAG223650.026.501736
TATCAAC227300.026.324162
GTACATG304100.022.792811
ACGCAGA266250.022.2614527
TACATGG307550.021.849392
CGCAGAG275150.021.5260098
ACATGGG308500.021.1325763
CATGGGG229800.018.759844
GCAGAGT334700.017.5446599
GAGTACT161850.016.96450212-13
AGAGTAC289850.016.88764810-11
CAGAGTA323350.016.15203910-11
GTGGTAT63500.015.66011
ATGGGGG137050.015.1876675
GTACTTT181950.015.18409414-15
Produced by FastQC (version 0.11.5)