##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10467634
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93718943554962	34.0	31.0	34.0	31.0	34.0
2	33.04509032318096	34.0	33.0	34.0	31.0	34.0
3	33.10888821676417	34.0	33.0	34.0	31.0	34.0
4	36.47270615308101	37.0	37.0	37.0	35.0	37.0
5	36.433241265409166	37.0	37.0	37.0	35.0	37.0
6	36.461340642976246	37.0	37.0	37.0	35.0	37.0
7	36.41882100577838	37.0	37.0	37.0	35.0	37.0
8	36.40216260904804	37.0	37.0	37.0	35.0	37.0
9	38.197639695847215	39.0	39.0	39.0	37.0	39.0
10-11	38.14468016363583	39.0	39.0	39.0	37.0	39.0
12-13	38.11811527800838	39.0	39.0	39.0	37.0	39.0
14-15	39.60272502840661	41.0	40.0	41.0	37.0	41.0
16-17	39.578846805304806	41.0	40.0	41.0	37.0	41.0
18-19	39.569275731268405	41.0	40.0	41.0	37.0	41.0
20-21	39.538951571802826	41.0	39.5	41.0	37.0	41.0
22-23	39.46009724405148	41.0	39.0	41.0	36.5	41.0
24-25	39.30899656683435	41.0	39.0	41.0	36.0	41.0
26-27	39.317416954354094	40.5	39.0	41.0	36.0	41.0
28-29	39.24484584492927	41.0	39.0	41.0	36.0	41.0
30-31	39.17862507162337	41.0	39.0	41.0	36.0	41.0
32-33	39.004103067972956	40.0	39.0	41.0	35.0	41.0
34-35	38.92798327904723	40.0	39.0	41.0	35.0	41.0
36-37	39.133126226938444	40.5	39.0	41.0	35.0	41.0
38-39	39.20998592895954	41.0	39.0	41.0	35.0	41.0
40-41	39.1906367368436	41.0	39.0	41.0	35.0	41.0
42-43	39.042825502026425	41.0	39.0	41.0	35.0	41.0
44-45	39.0660676268703	40.5	39.0	41.0	35.0	41.0
46-47	39.01846896092625	40.0	39.0	41.0	35.0	41.0
48-49	38.949465545219915	40.0	39.0	41.0	35.0	41.0
50-51	38.86815506632569	40.0	38.0	41.0	35.0	41.0
52-53	38.74446621050048	40.0	38.0	41.0	35.0	41.0
54-55	38.59664090635211	40.0	38.0	41.0	35.0	41.0
56-57	38.43707395757136	40.0	37.5	41.0	34.0	41.0
58-59	38.2632448268691	40.0	37.0	41.0	34.0	41.0
60-61	38.06356874269104	40.0	36.5	41.0	34.0	41.0
62-63	37.818702595604066	39.0	36.0	41.0	34.0	41.0
64-65	37.52806232891378	39.0	35.5	41.0	33.5	41.0
66-67	37.1901681123965	38.5	35.0	40.0	33.0	41.0
68-69	36.84123479421383	37.5	35.0	40.0	33.0	41.0
70-71	36.46954452466515	37.0	35.0	39.5	32.0	41.0
72-73	36.117417620681714	36.5	35.0	39.0	32.0	41.0
74-75	35.770774650245485	36.0	35.0	39.0	32.0	40.0
76-77	34.91008457544959	35.0	34.0	37.0	30.5	39.0
78-79	35.12220590210973	35.0	34.0	37.0	31.5	39.0
80-81	34.91026599386599	35.0	34.0	37.0	32.0	39.0
82-83	34.656032856045385	35.0	34.0	36.0	31.0	37.5
84-85	34.41391708979165	35.0	34.0	36.0	31.0	37.0
86-87	34.2181791094202	35.0	34.0	35.5	31.0	37.0
88-89	34.03942434706706	35.0	34.0	35.0	31.0	36.0
90-91	33.862454869369024	35.0	34.0	35.0	31.0	36.0
92-93	33.74320950673613	35.0	34.0	35.0	31.0	36.0
94-95	33.67923850528884	35.0	34.0	35.0	31.0	36.0
96-97	33.66039028905875	35.0	34.0	35.0	31.0	35.0
98-99	33.68546441118542	35.0	34.0	35.0	31.0	35.0
100-101	32.28537740763306	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	39.0
21	193.0
22	887.0
23	2923.0
24	7494.0
25	15446.0
26	27558.0
27	43682.0
28	63025.0
29	85508.0
30	111450.0
31	143049.0
32	184474.0
33	246326.0
34	357135.0
35	543013.0
36	1003646.0
37	2292736.0
38	3411516.0
39	1811717.0
40	115807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.641505043068946	20.18863097429658	13.99310484107488	25.17675914155959
2	14.159380239772165	21.00677399274533	41.19278691996146	23.64105884752104
3	17.529644234790783	25.658243305029583	30.50344519114826	26.30866726903138
4	12.027245125307209	18.015045233717572	38.33656201582898	31.621147625146236
5	13.285972742264393	37.818708602154025	35.536043770731766	13.359274884849814
6	30.883922766118875	37.563426462942815	18.53824847143108	13.014402299507225
7	27.621225217871793	32.07165255458163	22.453863455546358	17.853258772000217
8	24.97543363949174	36.8974028919426	20.43734904561385	17.689814422951812
9	25.751254802085715	16.571632029425693	20.412259291468033	37.264853877020556
10-11	24.452206678223558	26.398797474195216	28.914198757809075	20.23479708977215
12-13	25.45687974952124	23.754188386793043	28.769533783852207	22.019398079833515
14-15	22.44551364388436	25.384786170673873	26.000352330756805	26.16934785468496
16-17	22.317125935037616	28.57865493296618	26.648341596428683	22.455877535567517
18-19	22.35993348640199	27.89009913797139	28.063758247565783	21.686209128060842
20-21	23.133677437401694	27.002163675022278	27.912114745854165	21.95204414172186
22-23	22.848738400673536	26.95869586901365	27.71276912864174	22.479796601671076
24-25	22.693634545679103	27.0978737066763	27.75770858073438	22.450783166910217
26-27	22.59603648896893	27.20023290871912	27.78685429883831	22.416876303473636
28-29	22.55953124427404	27.18044915868366	27.82332001985414	22.43669957718815
30-31	22.786330480838693	27.135861768678577	27.670385767489357	22.40742198299337
32-33	22.667628178140365	27.233271917341597	27.611285733701084	22.487814170816954
34-35	22.667377187458726	27.271046380632363	27.720175661424463	22.34140077048445
36-37	22.704550371999517	27.166521703508508	27.646228530946598	22.48269939354538
38-39	22.513888367247493	27.26836430616669	27.759197601381636	22.458549725204186
40-41	22.75728301258921	27.134374854068977	27.664783837651324	22.443558295690487
42-43	22.70130442081256	27.179974175765985	27.503609774070814	22.615111629350636
44-45	22.675435488808198	27.267204730512745	27.41892255958404	22.638437221095018
46-47	22.791404551915054	27.208552632937632	27.193778888576198	22.806263926571116
48-49	22.679488763590268	27.2691872745468	27.26795625231866	22.783367709544272
50-51	22.648577190891093	27.38193194693582	27.224947864927667	22.744542997245425
52-53	22.832710161705634	27.279936110667492	27.143137665520577	22.744216062106297
54-55	22.81813205293954	27.217817373985532	27.133019357938327	22.831031215136605
56-57	22.808946398727215	27.371705344542985	27.083506660703765	22.735841596026034
58-59	22.721791853666236	27.398744735583115	27.156930859210597	22.722532551540052
60-61	22.809256432503656	27.421524639540817	27.05859493580952	22.710623992146004
62-63	22.79816468630635	27.54054952111132	27.0064428183766	22.65484297420573
64-65	22.7880041335521	27.410143806257835	27.00585359602396	22.7959984641661
66-67	22.84070485191901	27.454049305704515	26.887201576404035	22.818044265972436
68-69	22.761513713920305	27.37663417734305	27.023831935983424	22.83802017275322
70-71	22.742125220554698	27.223701293981467	27.059954762436924	22.974218723026908
72-73	22.811086758598993	27.23472249808554	26.97957986367441	22.97461087964106
74-75	22.708302497159977	27.38350831066663	26.969012118957007	22.939177073216385
76-77	22.84727561167645	27.273125632729805	26.947775366298423	22.93182338929532
78-79	22.761208398017942	27.28227923994087	26.890104344818194	23.06640801722299
80-81	22.783562604637307	27.355681580710943	26.890353310802535	22.97040250384921
82-83	22.818985371907633	27.349211708186928	26.957644262370305	22.874158657535137
84-85	22.859103203428294	27.308023521976484	26.907396639924997	22.92547663467023
86-87	22.771771788038674	27.30532533303995	27.02957509695434	22.893327781967038
88-89	22.808047795430895	27.2445297268473	27.003715781008804	22.943706696713008
90-91	22.800456591385636	27.349974968587592	26.955172086002637	22.89439635402413
92-93	22.852596948286454	27.27974101415951	26.99589254336644	22.871769494187596
94-95	22.69595470494456	27.319175430374703	27.048863973786126	22.936005890894613
96-97	22.7333247370259	27.326212195257487	27.058794912822627	22.88166815489399
98-99	22.909945960420544	27.86830728068241	27.08174531098404	22.140001447913008
100-101	20.797248954276707	31.625297624248343	25.01796006643538	22.559493355039567
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1809.0
1	1519.0
2	1802.5
3	2497.5
4	3189.5
5	4711.0
6	5991.5
7	7018.0
8	7646.0
9	7552.5
10	7073.5
11	6539.5
12	7218.0
13	9845.5
14	16058.0
15	24621.5
16	31767.0
17	36014.5
18	37205.0
19	35726.5
20	32741.5
21	29731.0
22	28323.5
23	30082.5
24	35480.5
25	44987.5
26	58330.5
27	74288.0
28	93404.0
29	115457.5
30	138293.5
31	163164.5
32	190904.0
33	219562.0
34	248634.5
35	277169.0
36	308565.0
37	335623.5
38	358355.0
39	381672.0
40	401626.0
41	419838.0
42	432899.0
43	446372.5
44	459297.0
45	468898.0
46	477208.5
47	480725.5
48	478660.5
49	473885.5
50	467671.0
51	454693.0
52	434419.5
53	410121.5
54	384503.0
55	357634.0
56	328298.5
57	297102.5
58	265155.0
59	230757.0
60	193496.0
61	157016.5
62	125252.5
63	97825.0
64	73191.0
65	54483.0
66	41430.5
67	31747.0
68	24373.5
69	18095.0
70	13021.5
71	9373.0
72	6706.0
73	4606.0
74	3003.5
75	1930.0
76	1288.0
77	839.5
78	529.5
79	326.5
80	227.5
81	168.0
82	131.0
83	91.0
84	60.0
85	42.5
86	32.0
87	24.0
88	14.5
89	9.0
90	6.0
91	3.5
92	2.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03057042307745953
3	0.0
4	0.0
5	0.0
6	0.0
7	9.553257211706102E-5
8	6.49621490396015E-4
9	4.4900308895018685E-4
10-11	0.0
12-13	0.0
14-15	0.0018819916707061024
16-17	6.687280048194271E-5
18-19	0.0
20-21	2.1972984883828354E-4
22-23	0.0019155886544222178
24-25	7.166665830555653E-4
26-27	0.014490168072090305
28-29	0.3145145021562535
30-31	0.3153573077714415
32-33	0.3326744493192318
34-35	0.21438879554161414
36-37	0.07123789122688275
38-39	0.062334005405298834
40-41	0.025402711440302355
42-43	0.010010842444015254
44-45	0.013451199342935335
46-47	0.009896875065475508
48-49	0.006627750630958808
50-51	0.006986964306212411
52-53	0.002785914870105425
54-55	0.0010501030276455557
56-57	0.003460712342266561
58-59	0.003825904506702367
60-61	0.0028364432035618407
62-63	0.0020274553326586745
64-65	6.588690611395898E-4
66-67	0.008309671308370237
68-69	0.012524917905928041
70-71	0.012729677852847421
72-73	0.010676205607648296
74-75	0.010988122906621899
76-77	0.010123825055610653
78-79	0.0026046218450998756
80-81	0.002107714288339278
82-83	0.0017503445838959913
84-85	1.845072803805229E-5
86-87	0.0029947591092976253
88-89	0.0
90-91	0.009524207317623103
92-93	0.02060315709952727
94-95	0.0014770539341628266
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	137.0
20-21	505.0
22-23	1190.0
24-25	2717.0
26-27	4446.0
28-29	8482.0
30-31	23002.0
32-33	30153.0
34-35	42755.0
36-37	64738.0
38-39	75510.0
40-41	74869.0
42-43	85904.0
44-45	100395.0
46-47	99641.0
48-49	92031.0
50-51	84916.0
52-53	83527.0
54-55	91081.0
56-57	94714.0
58-59	94005.0
60-61	91759.0
62-63	91118.0
64-65	96679.0
66-67	101868.0
68-69	100831.0
70-71	96647.0
72-73	92427.0
74-75	93660.0
76-77	95565.0
78-79	100607.0
80-81	100260.0
82-83	97450.0
84-85	98058.0
86-87	102448.0
88-89	108638.0
90-91	113652.0
92-93	117046.0
94-95	131916.0
96-97	205887.0
98-99	896404.0
100-101	6379996.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.59531615014875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.00922751696	77.04368285744859
2	7.038993955335498	12.05009825971856
3	1.6615668902270275	4.266670298208058
4	0.5965814005249191	2.0425829434891587
5	0.28167846585457956	1.2055178668755808
6	0.1438177996968049	0.7386078019840068
7	0.0807877409766842	0.48405365609689277
8	0.05223707264026335	0.3576998997921302
9	0.03155839006570641	0.2431125337377468
>10	0.10098960483519102	1.347726094224737
>50	0.0019930955441370326	0.11409033206046418
>100	5.141063965198189E-4	0.06828413837222712
>500	3.59752230539025E-5	0.020323093698569383
>1k	1.798571964332992E-5	0.017550224293269925
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9106514423412206E-5	0.0	0.0	0.0
3	0.0	1.9106514423412206E-5	0.0	9.553257211706103E-6	0.0
4	0.0	3.821302884682441E-5	0.0	2.8659771635118308E-5	0.0
5	0.0	3.821302884682441E-5	0.0	2.8659771635118308E-5	0.0
6	1.3374560096388543E-4	3.821302884682441E-5	0.0	2.8659771635118308E-5	1.9106514423412206E-5
7	1.3374560096388543E-4	4.776628605853052E-5	0.0	3.821302884682441E-5	1.9106514423412206E-5
8	1.3374560096388543E-4	5.7319543270236616E-5	0.0	3.821302884682441E-5	1.9106514423412206E-5
9	1.3374560096388543E-4	6.687280048194271E-5	0.0	4.776628605853052E-5	1.9106514423412206E-5
10-11	1.3374560096388543E-4	6.687280048194271E-5	0.0	6.687280048194271E-5	1.9106514423412206E-5
12-13	1.385222295697385E-4	6.687280048194271E-5	0.0	9.553257211706103E-5	1.9106514423412206E-5
14-15	2.0061840144582817E-4	9.553257211706103E-5	0.0	1.2419234375217933E-4	2.388314302926526E-5
16-17	3.295873738038606E-4	9.553257211706103E-5	0.0	1.289689723580324E-4	3.821302884682441E-5
18-19	3.343640024097136E-4	1.0986245793462018E-4	0.0	1.5285211538729765E-4	3.821302884682441E-5
20-21	3.3914063101556666E-4	1.2419234375217933E-4	0.0	1.6240537259900375E-4	3.821302884682441E-5
22-23	3.439172596214197E-4	1.8151188702241594E-4	0.0	1.767352584165629E-4	4.776628605853052E-5
24-25	3.4869388822727273E-4	2.1972491586924036E-4	0.0	2.1017165865753427E-4	5.7319543270236616E-5
26-27	3.630237740448319E-4	2.2927817308094646E-4	0.0	3.0092760216874227E-4	6.687280048194271E-5
28-29	3.72577031256538E-4	2.3883143029265256E-4	0.0	6.018552043374845E-4	6.687280048194271E-5
30-31	3.72577031256538E-4	2.865977163511831E-4	0.0	0.001485531496420299	6.687280048194271E-5
32-33	3.72577031256538E-4	2.865977163511831E-4	0.0	0.003921612085405355	7.164942908779578E-5
34-35	4.0123680289165634E-4	3.152574879863014E-4	0.0	0.007031197307815692	8.597931490535492E-5
36-37	4.0123680289165634E-4	3.439172596214197E-4	0.0	0.011263290252601496	8.597931490535492E-5
38-39	4.0123680289165634E-4	3.534705168331258E-4	0.0	0.01775472852795579	8.597931490535492E-5
40-41	4.0123680289165634E-4	3.7735365986239105E-4	0.0	0.024518434633843712	8.597931490535492E-5
42-43	4.2034331731506853E-4	3.8213028846824414E-4	0.0	0.031191384796220425	8.597931490535492E-5
44-45	4.346732031326277E-4	4.1079006010336246E-4	0.0	0.039020279081213576	8.597931490535492E-5
46-47	4.776628605853051E-4	4.537797175560399E-4	0.0	0.04682529022317746	8.597931490535492E-5
48-49	4.776628605853051E-4	4.63332974767746E-4	0.0	0.05488823930985741	8.597931490535492E-5
50-51	4.919927464028643E-4	5.349824038555417E-4	0.0	0.06222991747705355	8.597931490535492E-5
52-53	4.967693750087173E-4	5.349824038555417E-4	0.0	0.06938530712862143	8.597931490535492E-5
54-55	5.015460036145704E-4	5.349824038555417E-4	0.0	0.07700402975495704	8.597931490535492E-5
56-57	5.302057752496887E-4	5.349824038555417E-4	0.0	0.08422629220700685	8.597931490535492E-5
58-59	5.54088918278954E-4	5.397590324613948E-4	0.0	0.09145810791626838	9.075594351120797E-5
60-61	5.827486899140723E-4	5.445356610672479E-4	0.0	0.09847019870966064	9.553257211706103E-5
62-63	6.49621490396015E-4	5.445356610672479E-4	0.0	0.10526734121578954	9.553257211706103E-5
64-65	6.543981190018681E-4	6.209617187608967E-4	0.0	0.11162503389017996	9.553257211706103E-5
66-67	7.117176622721047E-4	6.352916045784558E-4	0.0	0.11829798405255668	9.553257211706103E-5
68-69	7.164942908779577E-4	6.591747476077211E-4	0.0	0.12507602004426216	9.553257211706103E-5
70-71	7.260475480896638E-4	6.591747476077211E-4	0.0	0.13132385026071794	1.1463908654047323E-4
72-73	7.547073197247821E-4	6.591747476077211E-4	0.0	0.1362198945817173	1.4329885817559155E-4
74-75	7.547073197247821E-4	6.878345192428394E-4	0.0	0.14003164420918807	1.4329885817559155E-4
76-77	7.547073197247821E-4	7.594839483306352E-4	0.0	0.14336095434746762	1.480754867814446E-4
78-79	7.547073197247821E-4	7.833670913599004E-4	0.0	0.1466711579713238	1.5285211538729765E-4
80-81	7.594839483306352E-4	7.833670913599004E-4	0.0	0.14820923238240846	1.576287439931507E-4
82-83	7.642605769364883E-4	7.929203485716065E-4	0.0	0.14884452398698694	1.7195862981070985E-4
84-85	7.642605769364883E-4	7.976969771774596E-4	0.0	0.14915022821776153	1.7195862981070985E-4
86-87	7.833670913599004E-4	8.072502343891657E-4	0.0	0.14925053741848443	1.767352584165629E-4
88-89	8.024736057833127E-4	8.215801202067248E-4	0.0	0.14929830370454297	1.9106514423412207E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10480	0.0	44.778618	1
GTATCAA	18070	0.0	34.072598	1
TCAACGC	20985	0.0	28.123554	4
ATCAACG	21520	0.0	27.483362	3
CAACGCA	21575	0.0	27.354475	5
AACGCAG	22365	0.0	26.50173	6
TATCAAC	22730	0.0	26.32416	2
GTACATG	30410	0.0	22.79281	1
ACGCAGA	26625	0.0	22.261452	7
TACATGG	30755	0.0	21.84939	2
CGCAGAG	27515	0.0	21.526009	8
ACATGGG	30850	0.0	21.132576	3
CATGGGG	22980	0.0	18.75984	4
GCAGAGT	33470	0.0	17.544659	9
GAGTACT	16185	0.0	16.964502	12-13
AGAGTAC	28985	0.0	16.887648	10-11
CAGAGTA	32335	0.0	16.152039	10-11
GTGGTAT	6350	0.0	15.6601	1
ATGGGGG	13705	0.0	15.187667	5
GTACTTT	18195	0.0	15.184094	14-15
>>END_MODULE