FastQCFastQC Report
Fri 27 May 2016
SRR2139659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8412317
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102450.051.915181
GTATCAA183150.036.2020451
TCAACGC227500.028.522734
ATCAACG230400.028.200443
CAACGCA235550.027.5838745
TATCAAC238250.027.4390582
AACGCAG241850.026.8828266
ACGCAGA279300.023.263097
CGCAGAG286150.022.7062058
GTACATG248650.022.173131
TACATGG251600.021.4928422
ACATGGG250200.021.150333
GCAGAGT328300.019.650199
GAGTACT189850.019.02884712-13
AGAGTAC294350.018.67572210-11
CATGGGG180650.018.3345784
CAGAGTA323200.017.95758610-11
GTGGTAT49300.017.937851
TGGTATC47850.016.978252
GTACTTT214950.016.72840114-15
Produced by FastQC (version 0.11.5)