FastQCFastQC Report
Fri 27 May 2016
SRR2139658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8121058
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73650.044.352271
GTATCAA127000.033.6222081
TCAACGC146450.028.432414
ATCAACG150850.027.6587433
CAACGCA151150.027.5760755
AACGCAG156700.026.6261736
TATCAAC158850.026.590252
GTACATG236900.022.9339161
TACATGG236500.022.564522
ACGCAGA189400.022.0291527
CGCAGAG196450.021.2172248
ACATGGG241350.021.1657963
CATGGGG176550.019.0676844
GCAGAGT241350.017.2012129
GAGTACT106250.016.67222412-13
AGAGTAC207300.016.54370710-11
CAGAGTA229450.015.95285710-11
GTGGTAT46100.015.8473751
TATACTG37750.015.7892935
ATGGGGG112950.015.6454185
Produced by FastQC (version 0.11.5)