##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139658_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8121058
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96098427076866	34.0	31.0	34.0	31.0	34.0
2	33.065270067028216	34.0	33.0	34.0	31.0	34.0
3	33.13015890294097	34.0	33.0	34.0	31.0	34.0
4	36.48793334563058	37.0	37.0	37.0	35.0	37.0
5	36.44981897678849	37.0	37.0	37.0	35.0	37.0
6	36.47668862850136	37.0	37.0	37.0	35.0	37.0
7	36.43262515795356	37.0	37.0	37.0	35.0	37.0
8	36.41605514946452	37.0	37.0	37.0	35.0	37.0
9	38.21651329174105	39.0	39.0	39.0	37.0	39.0
10-11	38.1628200414281	39.0	39.0	39.0	37.0	39.0
12-13	38.13766605287144	39.0	39.0	39.0	37.0	39.0
14-15	39.62955756503648	41.0	40.0	41.0	37.0	41.0
16-17	39.60670647839235	41.0	40.0	41.0	37.0	41.0
18-19	39.59388382646695	41.0	40.0	41.0	37.0	41.0
20-21	39.55887997013352	41.0	40.0	41.0	37.0	41.0
22-23	39.483650072493035	41.0	39.0	41.0	37.0	41.0
24-25	39.32987120258633	41.0	39.0	41.0	36.0	41.0
26-27	39.34059385967544	40.5	39.0	41.0	36.5	41.0
28-29	39.26568602834104	41.0	39.0	41.0	36.0	41.0
30-31	39.206376243688986	41.0	39.0	41.0	36.0	41.0
32-33	39.03696505756454	40.5	39.0	41.0	35.5	41.0
34-35	38.96339219984708	40.0	39.0	41.0	35.0	41.0
36-37	39.17862331299686	41.0	39.0	41.0	35.5	41.0
38-39	39.25725737431662	41.0	39.0	41.0	36.0	41.0
40-41	39.2377768303017	41.0	39.0	41.0	35.0	41.0
42-43	39.0896955853239	41.0	39.0	41.0	35.0	41.0
44-45	39.112949573283174	41.0	39.0	41.0	35.0	41.0
46-47	39.063800632069004	40.5	39.0	41.0	35.0	41.0
48-49	38.99038404078402	40.0	39.0	41.0	35.0	41.0
50-51	38.90577125987588	40.0	38.5	41.0	35.0	41.0
52-53	38.779511525339146	40.0	38.0	41.0	35.0	41.0
54-55	38.629624476663984	40.0	38.0	41.0	35.0	41.0
56-57	38.466441149807906	40.0	37.5	41.0	34.5	41.0
58-59	38.28633200111035	40.0	37.0	41.0	34.0	41.0
60-61	38.08462291562449	40.0	36.5	41.0	34.0	41.0
62-63	37.83486972139328	39.0	36.0	41.0	34.0	41.0
64-65	37.542596300882764	39.0	35.5	41.0	33.5	41.0
66-67	37.20231897130584	38.5	35.0	40.0	33.0	41.0
68-69	36.850233087860985	37.5	35.0	40.0	33.0	41.0
70-71	36.47621683311027	37.0	35.0	39.5	32.5	41.0
72-73	36.12249468454522	36.5	35.0	39.0	32.0	41.0
74-75	35.7757777642763	36.0	35.0	39.0	32.0	40.0
76-77	34.92032588742275	35.0	34.0	37.0	30.5	39.0
78-79	35.12432497708102	35.0	34.0	37.0	32.0	39.0
80-81	34.9119901299926	35.0	34.0	37.0	32.0	39.0
82-83	34.66458981846837	35.0	34.0	36.0	32.0	37.5
84-85	34.428684787534564	35.0	34.0	36.0	31.0	37.0
86-87	34.23317614059576	35.0	34.0	35.5	31.0	37.0
88-89	34.060228057431104	35.0	34.0	35.0	31.0	36.0
90-91	33.89002088602717	35.0	34.0	35.0	31.0	36.0
92-93	33.77496945024525	35.0	34.0	35.0	31.0	36.0
94-95	33.71443179243513	35.0	34.0	35.0	31.0	35.5
96-97	33.695934281829594	35.0	34.0	35.0	31.0	35.0
98-99	33.71805124727327	35.0	34.0	35.0	31.0	35.0
100-101	32.1555725566576	33.5	31.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	29.0
21	164.0
22	604.0
23	1971.0
24	5120.0
25	10296.0
26	19267.0
27	31078.0
28	45498.0
29	62346.0
30	82609.0
31	107000.0
32	138691.0
33	185953.0
34	273310.0
35	416470.0
36	768412.0
37	1744585.0
38	2644313.0
39	1485196.0
40	98140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3002098987595	19.754261082730846	13.634048667057913	25.311480351451742
2	14.42963799807013	20.647910265281844	41.15223818238567	23.77021355426235
3	17.815375779855287	25.188097412923295	30.169702026509356	26.826824780712073
4	12.12796411502048	17.54080564379666	37.9681440521666	32.36308618901626
5	13.414163524013741	37.75717400368277	35.46365510503681	13.36500736726668
6	31.604157980401077	37.16065074279731	18.24594775705333	12.989243519748289
7	28.216515192112944	31.83512094978781	22.07674716194544	17.8716166961538
8	25.236041778003255	36.99817264333791	20.109875360484274	17.655910218174565
9	25.965817360694583	16.40682460818052	19.9992414748733	37.6281165562516
10-11	24.77195705288646	26.11833335016201	28.824292352055608	20.285417244895925
12-13	25.728396472479325	23.468118316603576	28.62349954895039	22.179985661966704
14-15	22.616086414173818	25.179204496058926	25.69909209458569	26.505616995181562
16-17	22.621289806478973	28.341014293031247	26.360538971812225	22.677156928677558
18-19	22.68657605942477	27.695184543688768	27.670033879822064	21.9482055170644
20-21	23.494342020839746	26.85176286081927	27.466142355446483	22.187752762894497
22-23	23.188385807850974	26.743257782203173	27.29170382915965	22.776652580786205
24-25	23.010753549003717	26.88118135082607	27.36720949652551	22.740855603644704
26-27	22.941420288655078	27.01334798097187	27.359159406918494	22.686072323454557
28-29	22.886403473495537	27.04181213081174	27.348862518864152	22.722921876828572
30-31	23.104906524254787	26.929453027988536	27.280073335037848	22.685567112718825
32-33	22.9004821753123	27.043703688213277	27.274909303967405	22.78090483250702
34-35	22.961108704905538	27.040983812990465	27.29735922997829	22.70054825212571
36-37	22.98593043988715	26.966205551551113	27.299294375570298	22.748569632991437
38-39	22.814835787516426	27.077183423701662	27.366560627458554	22.74142016132336
40-41	23.083456087169182	26.93029576062657	27.256947246772462	22.72930090543179
42-43	23.017504461843462	26.969726451913367	27.145495172915375	22.867273913327793
44-45	22.98047364354653	27.06124168993594	27.077714030634947	22.88057063588258
46-47	23.06854429960922	26.99986511369783	26.873131852368925	23.058458734324024
48-49	22.934399594433025	27.02966234393205	26.977651745129133	23.05828631650579
50-51	22.90063464468806	27.165191768219866	26.972014215212543	22.962159371879533
52-53	23.127931100880588	27.01072900672169	26.880684906965207	22.980654985432516
54-55	23.04891528185978	26.967274440172805	26.89618441361652	23.087625864350894
56-57	23.0500076384847	27.151925188646647	26.840883357433576	22.95718381543507
58-59	22.989817154312977	27.15005869416825	26.909719739060563	22.950404412458212
60-61	23.079349020521644	27.193184653456083	26.80629957427833	22.921166751743943
62-63	23.071990568335448	27.2813368113773	26.733403694303938	22.913268925983317
64-65	23.02045638126442	27.19571959786451	26.783577604875052	23.000246415996017
66-67	23.07622257480027	27.232908289828785	26.647461835272228	23.04340730009872
68-69	23.006365303321953	27.169083322051478	26.77132862000493	23.053222754621636
70-71	22.95592767858101	27.01035039358281	26.83400487386371	23.199717053972474
72-73	23.052581537505567	27.010522296713045	26.76251651712353	23.174379648657855
74-75	22.931120134615472	27.145880884693852	26.747115869570813	23.175883111119862
76-77	23.025942259580763	27.059490767508677	26.717114951542225	23.197452021368335
78-79	23.010697742191226	27.054336908040753	26.666053200547402	23.268912149220615
80-81	23.029144775786108	27.101015588038813	26.64372704390365	23.226112592271434
82-83	23.061151308012086	27.088372987884284	26.740230194073007	23.110245510030627
84-85	23.11721497821555	27.034987806112554	26.716611797197924	23.131185418473972
86-87	23.024295806104345	27.022517906704905	26.795498985137556	23.15768730205319
88-89	23.03335220354633	27.035127606016474	26.753375709808726	23.178144480628468
90-91	23.023112804359318	27.124286292010257	26.73522739952047	23.11737350410996
92-93	23.076408219996587	27.06928083791617	26.767542459340028	23.086768482747214
94-95	22.944214066397187	27.070304876957387	26.82389121236392	23.161589844281508
96-97	22.92498286618468	27.057294025132432	26.883988606876812	23.133734501806074
98-99	23.163250247192337	27.630412405365917	26.878873909771105	22.327463437670637
100-101	24.460956184376197	36.36952880534163	28.657667257936858	10.511847752345316
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	987.0
1	858.0
2	1071.0
3	1463.5
4	1862.0
5	2799.0
6	3588.0
7	4163.0
8	4523.0
9	4483.0
10	4232.0
11	3960.0
12	4351.0
13	5817.5
14	9262.0
15	14171.5
16	18532.5
17	21408.5
18	22514.5
19	22021.5
20	20615.5
21	19055.5
22	18431.5
23	19727.0
24	23295.0
25	29910.0
26	38859.0
27	49494.0
28	62962.0
29	79167.0
30	95988.5
31	114452.5
32	136133.0
33	158669.5
34	182163.5
35	206297.5
36	233121.5
37	256753.5
38	274886.0
39	293153.5
40	311590.0
41	327063.5
42	337489.5
43	347917.0
44	357676.0
45	365071.5
46	370918.0
47	375225.5
48	379725.5
49	379806.5
50	374224.5
51	366076.5
52	353009.5
53	336043.5
54	318169.5
55	297573.5
56	274695.5
57	250716.5
58	223997.5
59	195091.0
60	164828.0
61	134966.0
62	107584.5
63	83457.5
64	62349.0
65	46307.5
66	34964.0
67	26650.5
68	20376.0
69	15037.0
70	10955.5
71	7897.5
72	5555.0
73	3877.0
74	2679.5
75	1753.5
76	1110.0
77	692.5
78	447.5
79	295.5
80	197.0
81	138.5
82	98.0
83	70.0
84	51.5
85	36.5
86	24.0
87	17.0
88	13.5
89	11.5
90	9.0
91	6.0
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.02920801698497905
3	0.0
4	0.0
5	0.0
6	0.0
7	2.4627333039611344E-5
8	4.679193277526155E-4
9	4.3097832819319847E-4
10-11	0.0
12-13	0.0
14-15	0.0020686959753273525
16-17	3.078416629951418E-5
18-19	0.0
20-21	1.8470878474364545E-4
22-23	0.0019333871767295055
24-25	6.527710191344429E-4
26-27	0.014112131634711509
28-29	0.31586066855552014
30-31	0.3159679008744413
32-33	0.33314451474581636
34-35	0.21570046464146173
36-37	0.07340494508533223
38-39	0.06455199034884951
40-41	0.025832240167184335
42-43	0.010276734404656847
44-45	0.013559830142845664
46-47	0.009772283033683602
48-49	0.006684939711637862
50-51	0.007009663112670113
52-53	0.002860754356134975
54-55	0.001014595222839269
56-57	0.003581586244904356
58-59	0.004145628465874948
60-61	0.0028288442369213624
62-63	0.0021107845630912447
64-65	7.27895993082419E-4
66-67	0.008484743888908346
68-69	0.012783651427467542
70-71	0.013420376881765814
72-73	0.011722508023854631
74-75	0.011850148997640192
76-77	0.010397861886970568
78-79	0.002932757912782573
80-81	0.002452310615007276
82-83	0.0020067300310254795
84-85	8.066073401590597E-6
86-87	0.0029260268535705822
88-89	0.0
90-91	0.009213172362805137
92-93	0.019511439703241516
94-95	0.0013901847051531193
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	103.0
20-21	336.0
22-23	790.0
24-25	2102.0
26-27	3154.0
28-29	6167.0
30-31	18525.0
32-33	24346.0
34-35	34022.0
36-37	53178.0
38-39	62329.0
40-41	61462.0
42-43	69538.0
44-45	79712.0
46-47	79557.0
48-49	75541.0
50-51	70191.0
52-53	69291.0
54-55	75798.0
56-57	78733.0
58-59	78412.0
60-61	76587.0
62-63	75233.0
64-65	80440.0
66-67	84049.0
68-69	82802.0
70-71	80197.0
72-73	76192.0
74-75	77203.0
76-77	79838.0
78-79	82856.0
80-81	83314.0
82-83	80497.0
84-85	80239.0
86-87	83930.0
88-89	88998.0
90-91	91971.0
92-93	93846.0
94-95	105115.0
96-97	160299.0
98-99	686142.0
100-101	4828023.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.56028120108485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.58160379770162	78.40769096374306
2	6.7449314262409255	11.676863218748977
3	1.5268260781246359	3.96487484002854
4	0.5309541964373807	1.838381781940629
5	0.25615122454904665	1.1086261013483856
6	0.11878289599337402	0.6169128527439403
7	0.06892610101701102	0.4176383880288798
8	0.04416599719523519	0.3058416909396706
9	0.026969935121023823	0.21010726512457528
>10	0.09833077361747866	1.2731378201563495
>50	0.0020729358488293186	0.11792747890866115
>100	2.1874821261717913E-4	0.023404291902544068
>500	6.588994085345904E-5	0.03859330638575902
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	6.156833259902835E-5	0.0	0.0	0.0
5	0.0	6.156833259902835E-5	0.0	0.0	0.0
6	3.694099955941701E-5	6.156833259902835E-5	0.0	1.231366651980567E-5	0.0
7	3.694099955941701E-5	6.156833259902835E-5	0.0	1.231366651980567E-5	0.0
8	3.694099955941701E-5	6.156833259902835E-5	0.0	1.231366651980567E-5	1.231366651980567E-5
9	3.694099955941701E-5	1.600776647574737E-4	0.0	8.619566563863969E-5	2.462733303961134E-5
10-11	8.003883237873685E-5	1.600776647574737E-4	0.0	9.850933215844536E-5	2.462733303961134E-5
12-13	9.850933215844536E-5	1.600776647574737E-4	0.0	9.850933215844536E-5	2.462733303961134E-5
14-15	1.1082299867825102E-4	1.908618310569879E-4	0.0	1.1082299867825102E-4	4.9254666079222675E-5
16-17	1.8470499779708505E-4	1.9701866431689073E-4	0.0	1.231366651980567E-4	7.388199911883402E-5
18-19	1.8470499779708505E-4	2.278028306164049E-4	0.0	1.4776399823766804E-4	8.619566563863969E-5
20-21	1.8470499779708505E-4	2.5858699691591906E-4	0.0	1.7854816453718222E-4	9.850933215844536E-5
22-23	1.8470499779708505E-4	3.1399849625504456E-4	0.0	1.8470499779708505E-4	9.850933215844536E-5
24-25	1.908618310569879E-4	3.324689960347531E-4	0.0	2.278028306164049E-4	9.850933215844536E-5
26-27	2.0317549757679356E-4	3.4478266255455876E-4	0.0	3.4478266255455876E-4	9.850933215844536E-5
28-29	2.093323308366964E-4	3.4478266255455876E-4	0.0	8.188588235670772E-4	9.850933215844536E-5
30-31	2.093323308366964E-4	4.925466607922268E-4	0.0	0.002314969305723466	1.1082299867825102E-4
32-33	2.2164599735650207E-4	5.048603273120325E-4	0.0	0.004962407607481685	1.3545033171786237E-4
34-35	2.5858699691591906E-4	6.34153825769992E-4	0.0	0.01031269571033725	1.4776399823766804E-4
36-37	2.955279964753361E-4	7.511336577081459E-4	0.0	0.019006144273320053	1.4776399823766804E-4
38-39	2.955279964753361E-4	7.696041574878545E-4	0.0	0.02985448447726885	1.4776399823766804E-4
40-41	2.955279964753361E-4	8.496429898665913E-4	0.0	0.04064741318187852	1.4776399823766804E-4
42-43	2.955279964753361E-4	8.557998231264941E-4	0.0	0.0497287422402352	1.4776399823766804E-4
44-45	3.0784166299514175E-4	9.912501548443565E-4	0.0	0.06204856559330077	1.4776399823766804E-4
46-47	3.201553295149474E-4	0.001126700486562219	0.0	0.07261369146729404	1.5392083149757087E-4
48-49	3.201553295149474E-4	0.0011328573198221216	0.0	0.08203364635494538	1.8470499779708505E-4
50-51	3.4478266255455876E-4	0.001348346483918721	0.0	0.0905608604199108	1.8470499779708505E-4
52-53	3.4478266255455876E-4	0.0013545033171786238	0.0	0.09868172348972265	1.8470499779708505E-4
54-55	3.4478266255455876E-4	0.0013545033171786238	0.0	0.10648858806327943	1.8470499779708505E-4
56-57	3.4478266255455876E-4	0.0013914443167380409	0.0	0.11363051464476673	1.8470499779708505E-4
58-59	3.4478266255455876E-4	0.0014283853162974577	0.0	0.11997820973572655	1.8470499779708505E-4
60-61	3.7556682885407297E-4	0.0014653263158568748	0.0	0.12651676665774336	1.908618310569879E-4
62-63	4.001941618936843E-4	0.001489953648896486	0.0	0.13237191508791096	2.093323308366964E-4
64-65	4.063509951535871E-4	0.0016623449801737656	0.0	0.13861494401345242	2.093323308366964E-4
66-67	4.063509951535871E-4	0.0016685018134336683	0.0	0.1450303642702712	2.093323308366964E-4
68-69	4.063509951535871E-4	0.0017362269792925997	0.0	0.15124876586277305	2.093323308366964E-4
70-71	4.2482149493329566E-4	0.0017731679788520166	0.0	0.15757799045395315	2.093323308366964E-4
72-73	4.49448827972907E-4	0.0017854816453718222	0.0	0.1628790238907295	2.3395966387630774E-4
74-75	4.556056612328098E-4	0.0018162658116713365	0.0	0.16672088784490888	2.4011649713621058E-4
76-77	4.8023299427242116E-4	0.001902461477309976	0.0	0.170008636805697	2.462733303961134E-4
78-79	5.048603273120325E-4	0.0019332456436094902	0.0	0.17341952243168318	2.462733303961134E-4
80-81	5.048603273120325E-4	0.0019332456436094902	0.0	0.1747924962486415	2.5243016365601625E-4
82-83	5.048603273120325E-4	0.0019578729766491013	0.0	0.1754697479072308	2.7090066343572473E-4
84-85	5.110171605719353E-4	0.00197634347642881	0.0	0.1759315104017235	2.770574966956276E-4
86-87	5.171739938318381E-4	0.002007127642728324	0.0	0.1760731175667013	2.832143299555304E-4
88-89	5.23330827091741E-4	0.002093323308366964	0.0	0.17618394056537953	2.832143299555304E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7365	0.0	44.35227	1
GTATCAA	12700	0.0	33.622208	1
TCAACGC	14645	0.0	28.43241	4
ATCAACG	15085	0.0	27.658743	3
CAACGCA	15115	0.0	27.576075	5
AACGCAG	15670	0.0	26.626173	6
TATCAAC	15885	0.0	26.59025	2
GTACATG	23690	0.0	22.933916	1
TACATGG	23650	0.0	22.56452	2
ACGCAGA	18940	0.0	22.029152	7
CGCAGAG	19645	0.0	21.217224	8
ACATGGG	24135	0.0	21.165796	3
CATGGGG	17655	0.0	19.067684	4
GCAGAGT	24135	0.0	17.201212	9
GAGTACT	10625	0.0	16.672224	12-13
AGAGTAC	20730	0.0	16.543707	10-11
CAGAGTA	22945	0.0	15.952857	10-11
GTGGTAT	4610	0.0	15.847375	1
TATACTG	3775	0.0	15.789293	5
ATGGGGG	11295	0.0	15.645418	5
>>END_MODULE