FastQCFastQC Report
Fri 27 May 2016
SRR2139657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8716850
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102200.049.149281
GTATCAA186900.038.0624471
TCAACGC228800.030.414724
ATCAACG232450.029.9554163
CAACGCA235850.029.505575
TATCAAC243000.028.7856882
AACGCAG243350.028.5962126
ACGCAGA280850.024.793087
CGCAGAG288000.024.1628078
GTACATG248650.021.9787541
TACATGG250400.021.3840162
GTGGTAT50650.020.9754411
GCAGAGT337250.020.559819
ACATGGG254000.020.2384683
TGGTATC51000.019.3317362
GAGTACT196550.019.15188612-13
AGAGTAC301550.018.95020110-11
CATGGGG183250.018.5237434
CAGAGTA328600.018.08197210-11
GTACTTT220050.017.12633114-15
Produced by FastQC (version 0.11.5)