##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139657_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8716850
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94907736166161	34.0	31.0	34.0	31.0	34.0
2	33.061645204402964	34.0	33.0	34.0	31.0	34.0
3	33.122513293219455	34.0	33.0	34.0	31.0	34.0
4	36.48690031376013	37.0	37.0	37.0	35.0	37.0
5	36.44880547445465	37.0	37.0	37.0	35.0	37.0
6	36.48031077740239	37.0	37.0	37.0	35.0	37.0
7	36.43802508933847	37.0	37.0	37.0	35.0	37.0
8	36.42230369915738	37.0	37.0	37.0	35.0	37.0
9	38.21783993070891	39.0	39.0	39.0	37.0	39.0
10-11	38.16960754171518	39.0	39.0	39.0	37.0	39.0
12-13	38.1424440021338	39.0	39.0	39.0	37.0	39.0
14-15	39.619513815197	41.0	40.0	41.0	37.0	41.0
16-17	39.59594148115431	41.0	40.0	41.0	37.0	41.0
18-19	39.59832858199923	41.0	40.0	41.0	37.0	41.0
20-21	39.571925402901016	41.0	40.0	41.0	37.0	41.0
22-23	39.49275233182347	41.0	39.0	41.0	37.0	41.0
24-25	39.33936561763157	41.0	39.0	41.0	36.0	41.0
26-27	39.34818715750385	40.5	39.0	41.0	36.5	41.0
28-29	39.27776180353866	41.0	39.0	41.0	36.0	41.0
30-31	39.198159001388206	41.0	39.0	41.0	36.0	41.0
32-33	39.010846088557884	40.0	39.0	41.0	35.0	41.0
34-35	38.93391832860774	40.0	38.5	41.0	35.0	41.0
36-37	39.131762167134966	40.5	39.0	41.0	35.0	41.0
38-39	39.20574777636212	41.0	39.0	41.0	35.0	41.0
40-41	39.18193171386939	41.0	39.0	41.0	35.0	41.0
42-43	39.036964214722815	41.0	39.0	41.0	35.0	41.0
44-45	39.05884511487358	40.5	39.0	41.0	35.0	41.0
46-47	39.012896575261884	40.0	39.0	41.0	35.0	41.0
48-49	38.94476510079677	40.0	39.0	41.0	35.0	41.0
50-51	38.8680097597677	40.0	38.0	41.0	35.0	41.0
52-53	38.74883791239888	40.0	38.0	41.0	35.0	41.0
54-55	38.60777741115276	40.0	38.0	41.0	35.0	41.0
56-57	38.456851898221515	40.0	37.5	41.0	34.5	41.0
58-59	38.29244483249446	40.0	37.0	41.0	34.0	41.0
60-61	38.10113076642651	40.0	37.0	41.0	34.0	41.0
62-63	37.86227642651319	39.0	36.0	41.0	34.0	41.0
64-65	37.57906768008801	39.0	35.5	41.0	33.5	41.0
66-67	37.25084002100587	38.5	35.0	40.0	33.0	41.0
68-69	36.90968956120432	37.5	35.0	40.0	33.0	41.0
70-71	36.54562166672994	37.0	35.0	39.5	32.5	41.0
72-73	36.20056894099873	36.5	35.0	39.0	32.0	41.0
74-75	35.85692342540665	36.0	35.0	39.0	32.0	40.0
76-77	34.99679392635285	35.0	34.0	37.0	30.5	39.0
78-79	35.220213958163114	35.0	34.0	37.0	32.0	39.0
80-81	35.00629051461998	35.0	34.0	37.0	32.0	39.0
82-83	34.74548361196593	35.0	34.0	36.5	31.5	38.5
84-85	34.4906271034624	35.0	34.0	36.0	31.0	37.0
86-87	34.281384347144126	35.0	34.0	36.0	31.0	37.0
88-89	34.08986767277514	35.0	34.0	35.0	31.0	36.5
90-91	33.900390102141415	35.0	34.0	35.0	31.0	36.0
92-93	33.77125116579843	35.0	34.0	35.0	31.0	36.0
94-95	33.698280965346854	35.0	34.0	35.0	31.0	36.0
96-97	33.67212292145634	35.0	34.0	35.0	31.0	35.5
98-99	33.69341378055722	35.0	34.0	35.0	31.0	35.0
100-101	32.279128577916175	34.0	31.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	0.0
19	5.0
20	31.0
21	147.0
22	640.0
23	2181.0
24	5429.0
25	11551.0
26	20654.0
27	33678.0
28	49565.0
29	68362.0
30	90909.0
31	117243.0
32	152124.0
33	204245.0
34	298081.0
35	456605.0
36	840472.0
37	1914742.0
38	2833560.0
39	1519145.0
40	97478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.75801803587719	20.87773011528938	14.777853187298895	24.58639866153454
2	13.826138675533	21.463883613085734	41.38415730534352	23.325820406037746
3	17.0044607867431	26.340782815072604	31.09048132994862	25.564275068235677
4	11.894666512482493	18.36667336633609	38.977747461008846	30.760912660172572
5	13.029732765788735	37.93419364430813	35.77735897195867	13.258714617944467
6	29.996106390750416	38.18184050014409	18.674995824178648	13.147057284926845
7	26.50196326857402	32.762327545036094	23.173911282768607	17.561797903621276
8	24.48690705400484	36.858865410707814	21.043335547832786	17.61089198745456
9	25.504345694268842	16.70623207697238	21.25808024032703	36.53134198843175
10-11	23.98371682337753	26.783185828779075	29.153686776397826	20.079410571445568
12-13	24.863216054333396	24.437018468485572	29.147011724075984	21.55275375310505
14-15	22.014745969426226	25.859423425436752	26.700534274088312	25.425296331048713
16-17	21.626160518733524	29.035084997204553	27.36981514700473	21.968939337057193
18-19	21.661856060388786	28.20104166069165	29.086826089699834	21.05027618921973
20-21	22.405297768907907	27.305666322172723	29.01975936743133	21.26927654148804
22-23	22.18126680175186	27.176504859292372	28.849167982353336	21.793060356602428
24-25	21.986907712251952	27.350402439104855	28.88541135653692	21.777278492106273
26-27	21.895643498125537	27.503732536461932	28.843215330403023	21.757408635009508
28-29	21.861757521893175	27.483646255080114	28.885902729426938	21.768693493599773
30-31	22.055942990395106	27.43950290481449	28.764800544991907	21.739753559798494
32-33	21.91475406575492	27.5749935330216	28.690424443085327	21.819827958138152
34-35	22.005822588168307	27.540798005458523	28.71517039074119	21.738209015631977
36-37	21.989236528617457	27.475376695054372	28.681642428156103	21.853744348172068
38-39	21.887409867634318	27.59458635022167	28.72954535672112	21.788458425422892
40-41	22.10832902915215	27.490884692016266	28.57659207511845	21.824194203713137
42-43	22.047710697280998	27.635354176752617	28.302141894618092	22.014793231348293
44-45	22.06870695866356	27.70782517527713	28.20189842663494	22.02156943942437
46-47	22.164705862433884	27.683279176674468	27.93566893990192	22.21634602098973
48-49	22.11520861251155	27.793242409563966	27.91175312490781	22.179795853016664
50-51	22.127925755398696	27.878363391924193	27.818417741743147	22.175293110933964
52-53	22.302430513160115	27.799831174233848	27.679186305788388	22.218552006817653
54-55	22.271439590799122	27.739062379573543	27.694316215831783	22.295181813795555
56-57	22.264263928159394	27.851266452116118	27.675534819252384	22.2089348004721
58-59	22.178755196171593	27.877993559070536	27.6914713597183	22.25177988503957
60-61	22.303745002795356	27.87523761162899	27.64515392105412	22.17586346452154
62-63	22.29577499768224	27.960478624423533	27.600449274395583	22.14329710349864
64-65	22.250503955683794	27.82219284526977	27.632698814348156	22.29460438469828
66-67	22.33105887608868	27.877243888513647	27.454405819123863	22.337291416273814
68-69	22.26060288524159	27.86831705453435	27.557567699971646	22.313512360252414
70-71	22.246925301815125	27.68947122843682	27.625319219394722	22.438284250353334
72-73	22.343720159295945	27.713310547509156	27.48201269503739	22.460956598157512
74-75	22.18376855018688	27.844145386955745	27.515344370053363	22.456741692804012
76-77	22.29904883666719	27.72483834847795	27.51046492027674	22.465647894578122
78-79	22.256102276440668	27.73089225561285	27.456855781401917	22.556149686544565
80-81	22.301399149881806	27.75502867044824	27.44396120155106	22.499610978118895
82-83	22.33671511258928	27.70944474305311	27.530783115757007	22.423057028600606
84-85	22.365094066532095	27.65771523679742	27.504692925503914	22.47249777116657
86-87	22.309091157993894	27.66545716633571	27.57003650700448	22.455415168665912
88-89	22.326294308561888	27.624562228200748	27.556217064061432	22.492926399175932
90-91	22.37357029575994	27.620246932383736	27.57570209421541	22.430480677640915
92-93	22.40366438584942	27.554124732845697	27.602537651535293	22.439673229769593
94-95	22.271831765651743	27.5278312411023	27.654495516379207	22.54584147686675
96-97	22.316464455752165	27.556136205999998	27.66953430192678	22.457865036321056
98-99	22.490400557048254	28.079813855307194	27.70143911039374	21.72834647725081
100-101	20.294656704471137	31.825833526124864	25.82041341243707	22.05909635696693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2070.0
1	1751.5
2	2113.5
3	2968.5
4	4051.5
5	6174.5
6	7879.5
7	9576.5
8	10986.5
9	11156.0
10	10351.5
11	9666.0
12	10795.0
13	14426.5
14	23052.0
15	34782.5
16	44276.5
17	49148.5
18	49199.5
19	46234.0
20	42289.0
21	38631.0
22	37073.0
23	39649.0
24	46616.0
25	57988.5
26	72976.0
27	89851.0
28	109082.0
29	130106.0
30	149720.5
31	169464.5
32	190978.5
33	213099.5
34	233259.5
35	252413.0
36	274342.0
37	292524.5
38	306061.0
39	319308.0
40	330711.5
41	340268.0
42	346356.5
43	351861.0
44	357731.0
45	361579.0
46	362558.5
47	360679.5
48	358690.0
49	355889.5
50	349955.0
51	340538.5
52	326821.0
53	309550.5
54	291700.0
55	271623.0
56	249724.5
57	226275.0
58	200921.5
59	174061.0
60	146564.5
61	119972.0
62	95041.5
63	73241.0
64	54356.5
65	40265.0
66	30383.5
67	22936.5
68	17460.0
69	12702.0
70	9009.5
71	6397.0
72	4447.5
73	3057.5
74	2042.5
75	1363.0
76	878.0
77	526.5
78	342.5
79	222.5
80	162.0
81	117.5
82	84.0
83	56.0
84	40.5
85	30.5
86	19.5
87	13.0
88	8.5
89	5.0
90	2.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.2944068097994114E-5
2	0.029104550382305534
3	2.2944068097994114E-5
4	2.2944068097994114E-5
5	2.2944068097994114E-5
6	2.2944068097994114E-5
7	1.032483064409735E-4
8	6.539059407928323E-4
9	6.997940769888205E-4
10-11	5.736017024498529E-6
12-13	1.1472034048997057E-5
14-15	0.0018699415499865205
16-17	5.162415322048675E-5
18-19	0.0
20-21	2.5238988845800964E-4
22-23	0.00194466582466652
24-25	7.401256056264234E-4
26-27	0.014439592029873199
28-29	0.3118683209373667
30-31	0.3121724667069504
32-33	0.3300326609587786
34-35	0.21386077757611965
36-37	0.07250861333796088
38-39	0.06413691893815819
40-41	0.02622439252218745
42-43	0.010068137215546958
44-45	0.013689968173217349
46-47	0.010012592528054457
48-49	0.007291871572431034
50-51	0.007616652640153033
52-53	0.0033925558005527628
54-55	0.001660486835942334
56-57	0.004021475437605765
58-59	0.0046144929415809655
60-61	0.003646262542241243
62-63	0.0024509767272283293
64-65	0.0010631240398661014
66-67	0.008088387243870777
68-69	0.01148384319073424
70-71	0.011890376148934682
72-73	0.010016748633182491
74-75	0.010534016579049085
76-77	0.009248050383154969
78-79	0.002238740723173989
80-81	0.0016360799495830176
82-83	0.0015251457869510116
84-85	0.0
86-87	0.0025972688275351344
88-89	0.0
90-91	0.008937049656482154
92-93	0.01838673945882211
94-95	0.001684232823331967
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	99.0
20-21	414.0
22-23	960.0
24-25	2453.0
26-27	4158.0
28-29	7691.0
30-31	19182.0
32-33	24842.0
34-35	35730.0
36-37	51473.0
38-39	59161.0
40-41	61185.0
42-43	72237.0
44-45	85954.0
46-47	84740.0
48-49	75569.0
50-51	67635.0
52-53	66289.0
54-55	71217.0
56-57	73485.0
58-59	72984.0
60-61	71120.0
62-63	70879.0
64-65	75812.0
66-67	79238.0
68-69	78822.0
70-71	77215.0
72-73	74081.0
74-75	75051.0
76-77	77127.0
78-79	81039.0
80-81	81854.0
82-83	79053.0
84-85	80426.0
86-87	84157.0
88-89	90628.0
90-91	96234.0
92-93	97294.0
94-95	108684.0
96-97	168853.0
98-99	745242.0
100-101	5386583.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.93159229089217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.86773424003852	78.99276825352169
2	6.568013117735721	11.419356770244665
3	1.482412030155373	3.8660531463774186
4	0.5075689115555675	1.7649509471552198
5	0.22461234042559514	0.9762954200690455
6	0.11174563431859133	0.5828535553722546
7	0.06924031727532028	0.42134197220291114
8	0.04378587462185736	0.3045100640581941
9	0.02726515179260896	0.21331827534459327
>10	0.09494211466907897	1.2639800418550324
>50	0.0023271610389717836	0.13121612199879215
>100	3.3281905297031036E-4	0.05256214465240522
>500	2.0287319771687513E-5	0.010793287147731843
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.2944068097994114E-5	0.0	0.0	0.0
3	0.0	3.441610214699117E-5	0.0	0.0	0.0
4	0.0	6.883220429398235E-5	0.0	0.0	0.0
5	0.0	6.883220429398235E-5	0.0	1.1472034048997057E-5	1.1472034048997057E-5
6	1.1472034048997057E-5	6.883220429398235E-5	0.0	1.1472034048997057E-5	1.1472034048997057E-5
7	1.1472034048997057E-5	8.030423834297941E-5	0.0	1.1472034048997057E-5	2.2944068097994114E-5
8	2.2944068097994114E-5	1.0324830644097352E-4	0.0	1.1472034048997057E-5	2.2944068097994114E-5
9	2.2944068097994114E-5	1.6060847668595882E-4	0.0	4.588813619598823E-5	3.441610214699117E-5
10-11	3.441610214699117E-5	1.6060847668595882E-4	0.0	1.0324830644097352E-4	3.441610214699117E-5
12-13	4.588813619598823E-5	1.6634449371045736E-4	0.0	1.204563575144691E-4	3.441610214699117E-5
14-15	5.162415322048676E-5	2.0649661288194704E-4	0.0	1.2619237453896764E-4	3.441610214699117E-5
16-17	6.309618726948381E-5	2.1223262990644555E-4	0.0	1.376644085879647E-4	3.441610214699117E-5
18-19	7.456822131848088E-5	2.409127150289382E-4	0.0	1.4913644263696174E-4	4.01521191714897E-5
20-21	8.604025536747793E-5	2.753288171759294E-4	0.0	1.7208051073495587E-4	5.7360170244985284E-5
22-23	9.177627239197646E-5	3.04008902298422E-4	0.0	2.1223262990644555E-4	6.309618726948381E-5
24-25	9.751228941647498E-5	3.326889874209147E-4	0.0	2.8106483420042795E-4	8.030423834297941E-5
26-27	1.0324830644097352E-4	3.4416102146991173E-4	0.0	3.326889874209147E-4	9.177627239197646E-5
28-29	1.0324830644097352E-4	3.5563305551890875E-4	0.0	8.604025536747793E-4	9.177627239197646E-5
30-31	1.0324830644097352E-4	3.900491576658999E-4	0.0	0.0022944068097994113	9.177627239197646E-5
32-33	1.0324830644097352E-4	4.072572087393955E-4	0.0	0.004915766589995239	9.177627239197646E-5
34-35	1.0324830644097352E-4	4.6461737898438084E-4	0.0	0.009791381060818989	1.2619237453896764E-4
36-37	1.0898432346547204E-4	5.162415322048676E-4	0.0	0.016605769285923243	1.2619237453896764E-4
38-39	1.3192839156346618E-4	5.506576343518588E-4	0.0	0.025949741018831345	1.376644085879647E-4
40-41	1.434004256124632E-4	6.653779748418293E-4	0.0	0.034754527151436586	1.376644085879647E-4
42-43	1.4913644263696174E-4	6.768500088908263E-4	0.0	0.04228018148757866	1.376644085879647E-4
44-45	1.6060847668595882E-4	7.456822131848087E-4	0.0	0.05260501213167601	1.376644085879647E-4
46-47	1.6634449371045736E-4	8.03042383429794E-4	0.0	0.062287408869029524	1.4913644263696174E-4
48-49	1.7208051073495587E-4	8.087784004542926E-4	0.0	0.07102909881436528	1.7208051073495587E-4
50-51	1.9502457883294996E-4	8.948186558217705E-4	0.0	0.07886449806983027	1.7208051073495587E-4
52-53	1.9502457883294996E-4	8.948186558217705E-4	0.0	0.08547238968205258	1.7208051073495587E-4
54-55	2.0649661288194704E-4	9.120267068952661E-4	0.0	0.09252195460516127	1.7208051073495587E-4
56-57	2.179686469309441E-4	9.177627239197645E-4	0.0	0.09886025341723215	1.7208051073495587E-4
58-59	2.2370466395544263E-4	9.407067920177587E-4	0.0	0.1048601272248576	1.7208051073495587E-4
60-61	2.2944068097994114E-4	9.693868771402513E-4	0.0	0.11050436797696415	1.7208051073495587E-4
62-63	2.2944068097994114E-4	0.0010038029792872427	0.0	0.11567825533306184	1.7208051073495587E-4
64-65	2.2944068097994114E-4	0.0010668991665567264	0.0	0.12116762362550693	1.7208051073495587E-4
66-67	2.3517669800443967E-4	0.0010783712006057235	0.0	0.126725824122246	1.7208051073495587E-4
68-69	2.638567831269323E-4	0.0010955792516792189	0.0	0.132444633095671	1.7781652775945438E-4
70-71	2.638567831269323E-4	0.0011013152687037175	0.0	0.1380372496945571	1.9502457883294996E-4
72-73	2.638567831269323E-4	0.0011070512857282162	0.0	0.14253428704176394	1.9502457883294996E-4
74-75	2.638567831269323E-4	0.0011185233197772131	0.0	0.1465839150610599	1.9502457883294996E-4
76-77	2.638567831269323E-4	0.0011414673878752074	0.0	0.14979608459477908	1.9502457883294996E-4
78-79	2.695928001514308E-4	0.0011472034048997058	0.0	0.15299678209444925	2.0649661288194704E-4
80-81	2.753288171759294E-4	0.0011529394219242043	0.0	0.1543390100781819	2.2370466395544263E-4
82-83	2.753288171759294E-4	0.0011701474729976999	0.0	0.1547806833890683	2.409127150289382E-4
84-85	2.753288171759294E-4	0.0011701474729976999	0.0	0.1551477884786362	2.409127150289382E-4
86-87	2.753288171759294E-4	0.001193091541095694	0.0	0.15533134102342017	2.5238474907793523E-4
88-89	2.753288171759294E-4	0.0012848678134876705	0.0	0.15536575712556716	2.695928001514308E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10220	0.0	49.14928	1
GTATCAA	18690	0.0	38.062447	1
TCAACGC	22880	0.0	30.41472	4
ATCAACG	23245	0.0	29.955416	3
CAACGCA	23585	0.0	29.50557	5
TATCAAC	24300	0.0	28.785688	2
AACGCAG	24335	0.0	28.596212	6
ACGCAGA	28085	0.0	24.79308	7
CGCAGAG	28800	0.0	24.162807	8
GTACATG	24865	0.0	21.978754	1
TACATGG	25040	0.0	21.384016	2
GTGGTAT	5065	0.0	20.975441	1
GCAGAGT	33725	0.0	20.55981	9
ACATGGG	25400	0.0	20.238468	3
TGGTATC	5100	0.0	19.331736	2
GAGTACT	19655	0.0	19.151886	12-13
AGAGTAC	30155	0.0	18.950201	10-11
CATGGGG	18325	0.0	18.523743	4
CAGAGTA	32860	0.0	18.081972	10-11
GTACTTT	22005	0.0	17.126331	14-15
>>END_MODULE