FastQCFastQC Report
Fri 27 May 2016
SRR2139656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10696242
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120150.048.7282031
GTATCAA219050.036.3442651
TCAACGC269200.028.7478684
ATCAACG272500.028.4775033
CAACGCA277550.027.867915
TATCAAC284350.027.5266322
AACGCAG284500.027.3048866
ACGCAGA331000.023.431057
CGCAGAG339100.022.8343078
GTACATG299300.021.8420411
TACATGG298250.021.4415232
ACATGGG305250.020.3596923
GCAGAGT397500.019.3955239
GAGTACT234200.018.07718712-13
AGAGTAC356950.018.05513810-11
CATGGGG211300.017.797364
GTGGTAT62950.017.3636821
CAGAGTA392500.017.15066310-11
TGGTATC62450.016.6979792
GTACTTT258500.016.39459614-15
Produced by FastQC (version 0.11.5)