##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139656_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10696242
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60131231137067	34.0	31.0	34.0	31.0	34.0
2	32.73375471497373	34.0	31.0	34.0	31.0	34.0
3	32.807076541461946	34.0	31.0	34.0	31.0	34.0
4	36.2832954789168	37.0	37.0	37.0	35.0	37.0
5	36.21610215999227	37.0	37.0	37.0	35.0	37.0
6	36.24290381612533	37.0	37.0	37.0	35.0	37.0
7	36.190234476744266	37.0	37.0	37.0	35.0	37.0
8	36.16919362893996	37.0	37.0	37.0	35.0	37.0
9	37.89250102980093	39.0	38.0	39.0	35.0	39.0
10-11	37.83064687579058	39.0	38.0	39.0	35.0	39.0
12-13	37.80024661932668	39.0	38.0	39.0	35.0	39.0
14-15	39.187190229989184	40.0	39.0	41.0	36.0	41.0
16-17	39.15015217494144	40.0	39.0	41.0	36.0	41.0
18-19	39.029320297727	40.0	38.5	41.0	36.0	41.0
20-21	39.08940949775493	40.0	39.0	41.0	36.0	41.0
22-23	39.00516790385793	40.0	38.5	41.0	35.0	41.0
24-25	38.89794203530409	40.0	38.0	41.0	35.0	41.0
26-27	38.84837269577908	40.0	38.0	41.0	35.0	41.0
28-29	38.73290937312428	40.0	38.0	41.0	35.0	41.0
30-31	38.6732390297274	40.0	38.0	41.0	35.0	41.0
32-33	38.55149452746754	40.0	38.0	41.0	34.5	41.0
34-35	38.325758213967404	40.0	38.0	41.0	34.0	41.0
36-37	38.5976951319006	40.0	38.0	41.0	34.5	41.0
38-39	38.6972615668538	40.0	38.0	41.0	35.0	41.0
40-41	38.69227573675769	40.0	38.0	41.0	35.0	41.0
42-43	38.62338767465808	40.0	38.0	41.0	34.5	41.0
44-45	38.55223436826583	40.0	38.0	41.0	34.0	41.0
46-47	38.48293269006723	40.0	38.0	41.0	34.0	41.0
48-49	38.414998131476125	40.0	38.0	41.0	34.0	41.0
50-51	38.33465499230216	40.0	38.0	41.0	34.0	41.0
52-53	38.23592683767313	40.0	37.5	41.0	33.5	41.0
54-55	38.11429980961505	40.0	37.0	41.0	33.0	41.0
56-57	37.96454265578889	40.0	37.0	41.0	33.0	41.0
58-59	37.79250707462479	40.0	36.5	41.0	33.0	41.0
60-61	37.62118297496963	39.0	36.0	41.0	33.0	41.0
62-63	37.40249645252857	39.0	35.5	41.0	33.0	41.0
64-65	37.11586717911719	38.5	35.0	40.0	32.0	41.0
66-67	36.79371957807459	38.0	35.0	40.0	32.0	41.0
68-69	36.47199367321174	37.5	35.0	40.0	31.0	41.0
70-71	36.12984973829077	37.0	35.0	39.5	31.0	41.0
72-73	35.80797491045588	36.0	35.0	39.0	31.0	41.0
74-75	35.47673219682481	36.0	34.0	39.0	31.0	40.0
76-77	34.55233259495331	35.0	33.5	37.0	29.5	39.0
78-79	34.79235296764386	35.0	34.0	37.0	30.0	39.0
80-81	34.5942579438003	35.0	34.0	37.0	30.0	39.0
82-83	34.34660345782459	35.0	34.0	36.0	30.0	38.5
84-85	34.090489090105166	35.0	34.0	36.0	30.0	37.0
86-87	33.886102370631164	35.0	34.0	35.5	30.0	37.0
88-89	33.687634162178284	35.0	34.0	35.0	30.0	36.5
90-91	33.50940207906068	35.0	33.0	35.0	30.0	36.0
92-93	33.37687676617374	35.0	33.0	35.0	29.0	36.0
94-95	33.32273043922566	35.0	33.0	35.0	29.0	36.0
96-97	33.314671779083916	35.0	33.0	35.0	29.5	35.0
98-99	33.39164997042667	35.0	33.0	35.0	30.0	35.0
100-101	31.79804376173916	33.5	30.0	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	55.0
21	295.0
22	1326.0
23	4508.0
24	11234.0
25	23555.0
26	41946.0
27	65906.0
28	95031.0
29	128754.0
30	166329.0
31	209865.0
32	265081.0
33	346699.0
34	480700.0
35	692100.0
36	1159933.0
37	2263248.0
38	3026164.0
39	1615583.0
40	97923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.312405235408846	21.286420034251282	14.864734735807211	24.53643999453266
2	13.771155401222778	21.875062816765958	41.27671342867489	23.077068353336372
3	16.86231817256214	26.656929436621272	31.387551541654336	25.093200849162255
4	11.838999155030336	18.68088811004837	39.208565026857094	30.271547708064194
5	12.758639903622226	38.245759585469365	36.02700836424606	12.968592146662349
6	29.593571274845875	38.67087150795578	18.796676440192734	12.938880777005608
7	26.129625713404764	33.00693832469385	23.45768728867578	17.405748673225606
8	24.21319562515508	37.13969822298336	21.22826876953607	17.418837382325496
9	25.35554889258313	16.76106241188819	21.43462647043122	36.44876222509746
10-11	23.760767439755814	26.973852987658347	29.44215555972736	19.823224012858475
12-13	24.64523521438651	24.606431866444307	29.40699640116594	21.34133651800324
14-15	21.798756958207793	26.120528439075592	26.899245627948893	25.181468974767725
16-17	21.416456682294445	29.208999523897784	27.602896627387256	21.771647166420514
18-19	21.44610930281706	28.401456268949126	29.289815293479105	20.86261913475471
20-21	22.194367903110788	27.57602835284738	29.161776760360425	21.067826983681407
22-23	21.913106246227798	27.481677211298912	28.99759373876521	21.607622803708082
24-25	21.708835088027104	27.623457583914195	29.072432520596802	21.5952748074619
26-27	21.68960586848322	27.77612858622551	28.979528456601393	21.554737088689873
28-29	21.677401077458246	27.80018581660065	28.97366621262991	21.548746893311193
30-31	21.855836832972003	27.70871562868873	28.90499950621	21.53044803212927
32-33	21.623415563267336	27.87362334143509	28.830777490931915	21.672183604365657
34-35	21.785038104288045	27.855137936691165	28.784470943448714	21.57535301557207
36-37	21.794276579871212	27.761610344055086	28.790198042658755	21.653915033414943
38-39	21.641698889930527	27.91313490526322	28.878433678143267	21.566732526662985
40-41	21.867627392522763	27.792348882821805	28.69903390951385	21.640989815141584
42-43	21.805869140205676	27.92536492600804	28.44952023671217	21.819245697074116
44-45	21.91354854089711	27.985243313204755	28.305139472176727	21.796068673721404
46-47	21.960002702888545	27.9413284796356	28.055561711253667	22.04310710622218
48-49	21.91513012280988	28.03534167840037	28.02389204216193	22.025636156627815
50-51	21.92249993898295	28.116839602324784	27.930603408508702	22.03005705018356
52-53	22.090126953372064	28.05898237464316	27.814173116469032	22.036717555515747
54-55	22.073237657429072	27.986840370473864	27.82397305532055	22.115948916776514
56-57	22.080542060627348	28.126643014642834	27.754650029635858	22.038164895093963
58-59	21.98411603764123	28.16088516163266	27.819544037700894	22.03545476302522
60-61	22.080295800840503	28.167332460051064	27.751912546764906	22.000459192343527
62-63	22.04875549585133	28.24096738935735	27.704302349176796	22.00597476561452
64-65	22.030932979524103	28.14332452511496	27.70817879761614	22.117563697744792
66-67	22.104640813213443	28.175483004675943	27.57571738039033	22.14415880172028
68-69	22.039129255758105	28.142086665011988	27.68163456175023	22.137149517479678
70-71	22.03445900204402	27.921826055279848	27.750364140674854	22.29335080200128
72-73	22.116587863602696	27.98588667998873	27.650298900984154	22.247226555424422
74-75	21.955243255077818	28.128278062148226	27.63817227926874	22.27830640350521
76-77	22.09943309795295	28.030498043047114	27.591239637510313	22.278829221489623
78-79	22.039582714404098	28.028894016364976	27.55715884314438	22.374364426086547
80-81	22.06507070677352	28.062700754667922	27.56030933669262	22.31191920186594
82-83	22.118162090717348	28.04853885652181	27.61456506472943	22.218733988031413
84-85	22.130822570349572	28.024978784634115	27.552687523268144	22.291511121748172
86-87	22.05390503387315	28.025265190394027	27.642910805549135	22.277918970183684
88-89	22.099775771660806	27.989000320398738	27.62707288081116	22.284151027129294
90-91	22.074082951269336	28.074755434644143	27.62908200988291	22.222079604203607
92-93	22.115271903448182	28.013854324035293	27.645857045964185	22.22501672655234
94-95	21.931267145169052	28.050412580599176	27.703771596193345	22.31454867803843
96-97	21.924323760708603	28.082162258021214	27.774247413152686	22.219266568117497
98-99	22.07874821219989	28.61294474613596	27.782535386063216	21.525771655600934
100-101	20.44990652118806	32.19911596062058	25.682917667053218	21.668059851138143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3715.0
1	3154.5
2	3518.5
3	4647.0
4	5764.5
5	8110.0
6	10205.5
7	12146.0
8	13586.5
9	13753.0
10	12985.0
11	12240.5
12	13651.5
13	18246.5
14	29050.5
15	43795.0
16	55415.0
17	61536.0
18	62564.0
19	60042.5
20	55806.0
21	51987.5
22	50740.0
23	54506.0
24	64467.5
25	80925.0
26	102346.0
27	125566.0
28	150605.5
29	176705.0
30	201198.5
31	225436.5
32	251093.0
33	277684.0
34	303434.5
35	329209.5
36	357698.5
37	379176.0
38	394276.5
39	409010.0
40	421792.5
41	431723.0
42	436614.5
43	442338.0
44	447100.5
45	449119.5
46	451310.5
47	449373.0
48	445980.5
49	440067.0
50	429270.5
51	415179.5
52	395552.0
53	372574.5
54	349085.5
55	323632.5
56	294033.0
57	262967.0
58	232732.5
59	200556.5
60	167006.5
61	136239.0
62	108594.5
63	83518.0
64	61613.0
65	45336.0
66	34234.0
67	26034.0
68	19537.5
69	14029.5
70	10010.5
71	7092.0
72	4849.0
73	3209.0
74	2103.0
75	1400.5
76	944.5
77	605.0
78	398.5
79	265.0
80	178.0
81	121.0
82	81.0
83	50.0
84	33.0
85	25.5
86	22.5
87	20.5
88	17.0
89	11.0
90	7.5
91	5.0
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004216434145749507
3	0.004898916834529361
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0029449595474747114
10-11	9.910022604200615E-4
12-13	0.0
14-15	5.702937536379599E-4
16-17	8.881624032066589E-5
18-19	3.272177274971901E-5
20-21	1.4024027272432344E-5
22-23	5.329557388673961E-4
24-25	0.004110166105419355
26-27	0.0208199242605676
28-29	0.3384156213774199
30-31	0.3310237494060739
32-33	0.34197115691884317
34-35	0.18247232814288672
36-37	0.04126437847905995
38-39	0.03787234014115776
40-41	0.014763783788932443
42-43	0.011827416932407573
44-45	0.01028639449131476
46-47	0.00638003598994661
48-49	0.01005501789001214
50-51	0.010794548651668725
52-53	0.0033577795356574207
54-55	0.004071259108861753
56-57	0.004317309978007268
58-59	0.005718030177075537
60-61	0.003925887134633478
62-63	0.002363490648491226
64-65	7.130273415504926E-4
66-67	0.006951245440654982
68-69	0.016168665271663964
70-71	0.013403366440306852
72-73	0.0141357698717226
74-75	0.007345614090448263
76-77	0.004598189062323948
78-79	0.0015108853960246887
80-81	0.001164847613014086
82-83	5.598452685042249E-4
84-85	9.250205477897682E-5
86-87	0.0
88-89	4.760259676608268E-4
90-91	0.0048179635351752615
92-93	0.01811796891072028
94-95	0.0016447858883254568
96-97	6.841791440987325E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	181.0
20-21	680.0
22-23	1520.0
24-25	3496.0
26-27	5960.0
28-29	11005.0
30-31	26139.0
32-33	34199.0
34-35	49024.0
36-37	69205.0
38-39	80768.0
40-41	84175.0
42-43	98961.0
44-45	115447.0
46-47	114066.0
48-49	102480.0
50-51	93043.0
52-53	91588.0
54-55	99288.0
56-57	102242.0
58-59	101005.0
60-61	99912.0
62-63	99673.0
64-65	106623.0
66-67	110452.0
68-69	108445.0
70-71	106744.0
72-73	102850.0
74-75	104082.0
76-77	106409.0
78-79	111180.0
80-81	111730.0
82-83	108548.0
84-85	110012.0
86-87	116868.0
88-89	123642.0
90-91	131542.0
92-93	137471.0
94-95	158481.0
96-97	240634.0
98-99	920136.0
100-101	6196336.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.83435090603707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.18962003435517	77.41367494110993
2	6.872991363665158	11.79877504965995
3	1.65839062226756	4.270406478329846
4	0.599124143590104	2.0570172790876775
5	0.27591578722636395	1.184152625065159
6	0.13965933315322027	0.7192540925505988
7	0.08356591413450505	0.5020978198323409
8	0.04605540456555265	0.31625086052793183
9	0.030997201728780583	0.23945622212640344
>10	0.10142651145048419	1.3135778759433863
>50	0.0018796364944526382	0.1036538866825893
>100	3.3968475908711205E-4	0.05990129474961239
>500	3.436260948889808E-5	0.02178157433455706
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8698155856982295E-5	0.0	9.349077928491147E-6	0.0
3	0.0	1.8698155856982295E-5	0.0	9.349077928491147E-6	0.0
4	0.0	4.674538964245573E-5	0.0	9.349077928491147E-6	0.0
5	0.0	4.674538964245573E-5	0.0	9.349077928491147E-6	0.0
6	8.414170135642032E-5	4.674538964245573E-5	0.0	9.349077928491147E-6	0.0
7	8.414170135642032E-5	4.674538964245573E-5	0.0	1.8698155856982295E-5	0.0
8	8.414170135642032E-5	4.674538964245573E-5	0.0	1.8698155856982295E-5	9.349077928491147E-6
9	8.414170135642032E-5	8.414170135642032E-5	0.0	1.8698155856982295E-5	9.349077928491147E-6
10-11	8.414170135642032E-5	8.88162403206659E-5	0.0	2.804723378547344E-5	9.349077928491147E-6
12-13	8.414170135642032E-5	9.349077928491146E-5	0.0	2.804723378547344E-5	9.349077928491147E-6
14-15	1.0751439617764818E-4	1.215380130703849E-4	0.0	3.272177274971902E-5	3.739631171396459E-5
16-17	1.6828340271284064E-4	1.215380130703849E-4	0.0	3.739631171396459E-5	3.739631171396459E-5
18-19	1.6828340271284064E-4	1.589343247843495E-4	0.0	4.207085067821016E-5	3.739631171396459E-5
20-21	1.6828340271284064E-4	2.1502879235529637E-4	0.0	5.609446757094688E-5	3.739631171396459E-5
22-23	1.7295794167708622E-4	2.617741819977521E-4	0.0	6.076900653519245E-5	3.739631171396459E-5
24-25	1.776324806413318E-4	3.131941106044534E-4	0.0	8.88162403206659E-5	3.739631171396459E-5
26-27	1.776324806413318E-4	3.365668054256813E-4	0.0	1.3088709099887605E-4	3.739631171396459E-5
28-29	1.776324806413318E-4	3.365668054256813E-4	0.0	4.581048184960662E-4	5.14199286067013E-5
30-31	1.776324806413318E-4	3.7396311713964585E-4	0.0	0.001435083462023391	5.609446757094688E-5
32-33	1.776324806413318E-4	3.83312195068137E-4	0.0	0.0034965551452556887	5.609446757094688E-5
34-35	1.776324806413318E-4	4.6277935746031175E-4	0.0	0.006376071147230962	5.609446757094688E-5
36-37	1.776324806413318E-4	5.422465198524865E-4	0.0	0.010872977630835204	7.479262342792918E-5
38-39	1.776324806413318E-4	5.515955977809777E-4	0.0	0.01761366281727732	8.414170135642032E-5
40-41	1.776324806413318E-4	5.889919094949422E-4	0.0	0.024344998925790947	8.414170135642032E-5
42-43	2.1502879235529637E-4	6.030155263876789E-4	0.0	0.030922075248484466	8.414170135642032E-5
44-45	2.2437787028378752E-4	6.871572277440993E-4	0.0	0.0389061877994159	8.414170135642032E-5
46-47	2.3372694821227868E-4	7.572753122077829E-4	0.0	0.04773171736391155	8.88162403206659E-5
48-49	2.3372694821227868E-4	7.619498511720285E-4	0.0	0.05556157012902288	9.349077928491146E-5
50-51	2.3372694821227868E-4	8.507660914926944E-4	0.0	0.06399443842052191	1.0283985721340262E-4
52-53	2.3372694821227868E-4	8.694642473496767E-4	0.0	0.0715438188477785	1.0283985721340262E-4
54-55	2.3372694821227868E-4	8.694642473496767E-4	0.0	0.07940639338563955	1.0283985721340262E-4
56-57	2.3372694821227868E-4	8.694642473496767E-4	0.0	0.0866519287802202	1.0751439617764818E-4
58-59	2.5242510406926094E-4	8.694642473496767E-4	0.0	0.09322900510291371	1.1218893514189376E-4
60-61	2.5709964303350654E-4	8.975114811351501E-4	0.0	0.0996892179515011	1.1218893514189376E-4
62-63	2.7112325992624325E-4	9.162096369921324E-4	0.0	0.10583623668948403	1.1218893514189376E-4
64-65	2.7112325992624325E-4	0.0010564458059194996	0.0	0.11228710046014292	1.1218893514189376E-4
66-67	2.804723378547344E-4	0.0010657948838479908	0.0	0.11906518195829899	1.215380130703849E-4
68-69	2.804723378547344E-4	0.0011172148124546921	0.0	0.1255487675017076	1.2621255203463047E-4
70-71	2.804723378547344E-4	0.0011452620462401655	0.0	0.13212116928543688	1.3088709099887605E-4
72-73	2.8982141578322556E-4	0.0011546111241686565	0.0	0.1371089023602869	1.3088709099887605E-4
74-75	2.8982141578322556E-4	0.0011779838189898847	0.0	0.1415403652983917	1.3088709099887605E-4
76-77	2.8982141578322556E-4	0.001215380130703849	0.0	0.14549969980110772	1.3088709099887605E-4
78-79	2.8982141578322556E-4	0.0012387528255250768	0.0	0.1489869058684349	1.3088709099887605E-4
80-81	2.8982141578322556E-4	0.0012434273644893225	0.0	0.15035187124599464	1.3088709099887605E-4
82-83	2.8982141578322556E-4	0.0012434273644893225	0.0	0.1509268395385968	1.3088709099887605E-4
84-85	2.991704937117167E-4	0.0012434273644893225	0.0	0.15137092074020014	1.3088709099887605E-4
86-87	2.991704937117167E-4	0.001262125520346305	0.0	0.1515064823701633	1.3088709099887605E-4
88-89	2.991704937117167E-4	0.0012995218320602693	0.0	0.15155790229876998	1.3088709099887605E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12015	0.0	48.728203	1
GTATCAA	21905	0.0	36.344265	1
TCAACGC	26920	0.0	28.747868	4
ATCAACG	27250	0.0	28.477503	3
CAACGCA	27755	0.0	27.86791	5
TATCAAC	28435	0.0	27.526632	2
AACGCAG	28450	0.0	27.304886	6
ACGCAGA	33100	0.0	23.43105	7
CGCAGAG	33910	0.0	22.834307	8
GTACATG	29930	0.0	21.842041	1
TACATGG	29825	0.0	21.441523	2
ACATGGG	30525	0.0	20.359692	3
GCAGAGT	39750	0.0	19.395523	9
GAGTACT	23420	0.0	18.077187	12-13
AGAGTAC	35695	0.0	18.055138	10-11
CATGGGG	21130	0.0	17.79736	4
GTGGTAT	6295	0.0	17.363682	1
CAGAGTA	39250	0.0	17.150663	10-11
TGGTATC	6245	0.0	16.697979	2
GTACTTT	25850	0.0	16.394596	14-15
>>END_MODULE