FastQCFastQC Report
Fri 27 May 2016
SRR2139655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9139362
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110150.046.8094671
GTATCAA197600.034.8896831
TCAACGC244400.027.7133354
ATCAACG246550.027.455213
CAACGCA250250.027.032185
TATCAAC256850.026.549252
AACGCAG257150.026.3392546
ACGCAGA298250.022.7235777
CGCAGAG305800.022.1625468
GTACATG265750.022.1310621
TACATGG270150.021.3077322
ACATGGG264250.021.262713
GCAGAGT353750.019.099979
AGAGTAC321300.018.1680310-11
CATGGGG179200.018.026044
GAGTACT210750.017.75990512-13
GTGGTAT59300.017.64281
CAGAGTA346800.016.868210-11
AGTACTT229850.016.32943312-13
TGGTATC58800.016.3041822
Produced by FastQC (version 0.11.5)