##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9139362
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.568063284942646	34.0	31.0	34.0	31.0	34.0
2	32.699582531034444	34.0	31.0	34.0	31.0	34.0
3	32.77271925545787	34.0	31.0	34.0	31.0	34.0
4	36.26074489663502	37.0	37.0	37.0	35.0	37.0
5	36.19120065492537	37.0	35.0	37.0	35.0	37.0
6	36.218468750882174	37.0	37.0	37.0	35.0	37.0
7	36.16549721960898	37.0	37.0	37.0	35.0	37.0
8	36.144225165826676	37.0	36.0	37.0	35.0	37.0
9	37.86036596427628	39.0	38.0	39.0	35.0	39.0
10-11	37.79700229622155	39.0	38.0	39.0	35.0	39.0
12-13	37.764898906510105	39.0	38.0	39.0	35.0	39.0
14-15	39.136379268049566	40.0	38.5	41.0	36.0	41.0
16-17	39.10008844162208	40.0	38.0	41.0	36.0	41.0
18-19	38.9865660206916	40.0	38.5	41.0	35.0	41.0
20-21	39.04565498091402	40.0	39.0	41.0	36.0	41.0
22-23	38.95963177833585	40.0	38.0	41.0	35.0	41.0
24-25	38.851741604194046	40.0	38.0	41.0	35.0	41.0
26-27	38.8000325725474	40.0	38.0	41.0	35.0	41.0
28-29	38.683791111970166	40.0	38.0	41.0	35.0	41.0
30-31	38.62238631074244	40.0	38.0	41.0	35.0	41.0
32-33	38.49759530244003	40.0	38.0	41.0	34.0	41.0
34-35	38.26312566942217	40.0	38.0	41.0	34.0	41.0
36-37	38.542844322984024	40.0	38.0	41.0	34.0	41.0
38-39	38.644614509749815	40.0	38.0	41.0	35.0	41.0
40-41	38.642772956708704	40.0	38.0	41.0	35.0	41.0
42-43	38.57266322189734	40.0	38.0	41.0	34.0	41.0
44-45	38.497828949440716	40.0	38.0	41.0	34.0	41.0
46-47	38.42564060232982	40.0	38.0	41.0	34.0	41.0
48-49	38.35723798577679	40.0	38.0	41.0	34.0	41.0
50-51	38.28070515676917	40.0	38.0	41.0	33.5	41.0
52-53	38.18319123808326	40.0	37.5	41.0	33.0	41.0
54-55	38.0614405526152	40.0	37.0	41.0	33.0	41.0
56-57	37.91297253397187	40.0	37.0	41.0	33.0	41.0
58-59	37.73902307238977	39.5	36.0	41.0	33.0	41.0
60-61	37.56719555596873	39.0	36.0	41.0	33.0	41.0
62-63	37.347127533870236	39.0	35.5	41.0	32.5	41.0
64-65	37.057635353813055	38.5	35.0	40.0	32.0	41.0
66-67	36.73154239505958	38.0	35.0	40.0	31.5	41.0
68-69	36.41040697200876	37.0	35.0	40.0	31.0	41.0
70-71	36.06842229493277	37.0	35.0	39.5	31.0	41.0
72-73	35.74958776825045	36.0	34.5	39.0	31.0	41.0
74-75	35.42254225620641	36.0	34.0	39.0	31.0	40.0
76-77	34.50741082777651	35.0	33.5	37.0	29.5	39.0
78-79	34.75098902150891	35.0	34.0	37.0	30.0	39.0
80-81	34.5597735818298	35.0	34.0	37.0	30.0	39.0
82-83	34.31231662414402	35.0	34.0	36.0	30.0	38.5
84-85	34.058157603035546	35.0	34.0	36.0	30.0	37.0
86-87	33.85403036669955	35.0	34.0	35.5	30.0	37.0
88-89	33.65487621477534	35.0	33.5	35.0	30.0	36.5
90-91	33.47550897246391	35.0	33.0	35.0	29.0	36.0
92-93	33.343198537299784	35.0	33.0	35.0	29.0	36.0
94-95	33.28969081905289	35.0	33.0	35.0	29.0	36.0
96-97	33.28159868734753	35.0	33.0	35.0	29.0	35.0
98-99	33.361479211339855	35.0	33.0	35.0	30.0	35.0
100-101	31.79151613821812	33.5	30.0	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	0.0
18	2.0
19	10.0
20	43.0
21	259.0
22	1071.0
23	3753.0
24	9789.0
25	20453.0
26	35914.0
27	57215.0
28	83357.0
29	113008.0
30	146079.0
31	185712.0
32	235385.0
33	304632.0
34	421290.0
35	604619.0
36	1004804.0
37	1927595.0
38	2538611.0
39	1365837.0
40	79922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.279216487806586	21.25654312774636	14.699803925001312	24.764436459445736
2	13.876592090017636	21.790524552497978	41.06000989822857	23.272873459255813
3	17.001405088365406	26.51820065966169	31.212482603264036	25.267911648708868
4	11.92680887939637	18.259429544300694	38.941413840794105	30.872347735508832
5	12.743430612209169	38.17827506521244	35.98943470877611	13.088859613802278
6	30.002582237705923	38.392305369303756	18.45962955830605	13.145482834684266
7	26.141141173999056	32.900607920029415	23.45888552371282	17.499365382258713
8	24.234208033339748	37.181917074736724	21.23574927877898	17.34812561314455
9	25.518409682174532	16.461011932898455	21.505281042868095	36.515297342058915
10-11	23.989750598666816	26.929560149007596	29.175145420603	19.905543831722582
12-13	24.596878271905442	24.611179052890023	29.325939745678063	21.466002929526475
14-15	21.800713293530848	25.970586352667247	26.88986404312368	25.338836310678232
16-17	21.38948185246959	29.092513242042806	27.661236324461136	21.856768581026465
18-19	21.50791234504176	28.279351146948787	29.31010194097342	20.90263456703603
20-21	22.183806027419266	27.491957483054513	29.22387037521424	21.100366114311985
22-23	21.92605690000656	27.373432084045234	29.0664374352763	21.634073580671902
24-25	21.71028400625066	27.483438888313046	29.167909418164633	21.63836768727166
26-27	21.656734206737127	27.666228827691207	29.088105835658553	21.588931129913117
28-29	21.63216223014117	27.70040217188649	29.08923304159089	21.578202556381456
30-31	21.820499708537298	27.62023868566009	28.987977645168517	21.571283960634094
32-33	21.631105865964166	27.808043006867837	28.90845648178186	21.652394645386135
34-35	21.83042331479394	27.780621261863597	28.871394053332278	21.51756137001018
36-37	21.768433971575853	27.696144863149524	28.889178192604486	21.646242972670134
38-39	21.63671004144137	27.859655860857334	28.916410714065766	21.587223383635532
40-41	21.855698872752928	27.72124339123984	28.790521113431517	21.632536622575714
42-43	21.78114005264375	27.809082698066124	28.567399829380495	21.842377419909635
44-45	21.86090887989097	27.916182891627273	28.40687510111721	21.816033127364545
46-47	21.94217858030806	27.875424114076054	28.18632265454809	21.996074651067797
48-49	21.876246822720006	28.003162399156533	28.133801426556726	21.98678935156673
50-51	21.898194950234544	28.10159064360085	28.00236227947582	21.99785212668879
52-53	22.077792980820654	28.01743534375317	27.89308617286892	22.011685502557253
54-55	22.05139240111171	27.93801854696586	27.922239736014053	22.088349315908378
56-57	22.017837385781654	28.05552336273738	27.900926220117356	22.02571303136361
58-59	21.967665475642974	28.116947478896414	27.869253031400447	22.04613401406016
60-61	22.069440198465827	28.0668368906686	27.85762100035089	22.006101910514683
62-63	22.061088713270767	28.14255957049256	27.844966365606115	21.951385350630556
64-65	22.026416690807864	28.050277299976546	27.85144102757252	22.07186498164307
66-67	22.064530096125168	28.102925132119864	27.695364649269422	22.137180122485546
68-69	22.031363339801622	28.077961980218475	27.792490653219033	22.098184026760865
70-71	22.03531086950606	27.8437990006895	27.877112899251973	22.24377723055247
72-73	22.085821839306927	27.95151880116572	27.756311484326663	22.206347875200688
74-75	21.956782713903657	28.030247096262535	27.762376032425408	22.250594157408404
76-77	22.087688090657643	27.93469054733786	27.75174069495266	22.22588066705184
78-79	22.031160557484252	27.940280772840676	27.702916550644158	22.325642119030913
80-81	22.053118996755543	27.942195025434014	27.70019723642155	22.304488741388894
82-83	22.0864075225403	27.925074753389822	27.759665291117564	22.228852432952316
84-85	22.10671005823425	27.895144396439125	27.72744194336357	22.270703601963053
86-87	22.055138863430756	27.866353186733917	27.82477102463887	22.253736925196456
88-89	22.078527306583233	27.84942967160736	27.80072548152337	22.271317540286038
90-91	22.132493539824885	27.831437244525624	27.826552712571395	22.209516503078095
92-93	22.152497618473184	27.74959084996257	27.863405135641322	22.234506395922928
94-95	21.974326340165298	27.74066342504135	27.960769319261853	22.3242409155315
96-97	21.986869140079314	27.798212712536518	27.962984913731948	22.251933233652217
98-99	22.106877081978134	28.352794522536406	28.00686650039104	21.533461895094415
100-101	20.500037648370945	31.925086371101724	25.935740117995238	21.639135862532093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2481.0
1	2131.5
2	2611.5
3	3641.0
4	4744.0
5	6967.5
6	8901.5
7	11035.0
8	12986.5
9	13412.5
10	12479.5
11	11423.5
12	12525.5
13	16624.0
14	26171.0
15	39359.0
16	49818.0
17	54869.0
18	55353.0
19	53050.5
20	49694.5
21	46956.0
22	46621.0
23	51063.0
24	60969.0
25	75323.5
26	92496.5
27	110836.0
28	130009.5
29	149867.0
30	168626.0
31	187845.0
32	209678.5
33	232724.5
34	254705.0
35	275050.5
36	297354.5
37	315132.5
38	328051.0
39	341343.0
40	353711.5
41	364439.5
42	369781.5
43	374935.5
44	380751.5
45	383564.5
46	383651.5
47	381141.5
48	378585.0
49	374294.5
50	367489.5
51	356748.5
52	341647.0
53	323225.5
54	302844.5
55	281753.0
56	259111.5
57	234537.0
58	208141.0
59	180803.0
60	152974.0
61	126102.0
62	100821.5
63	78222.0
64	58778.5
65	43881.5
66	32778.5
67	24684.0
68	18806.0
69	13609.0
70	9779.0
71	7011.0
72	4863.5
73	3331.0
74	2267.5
75	1454.5
76	935.5
77	605.0
78	409.0
79	292.5
80	197.0
81	115.5
82	80.5
83	64.5
84	52.5
85	39.0
86	26.5
87	21.0
88	16.0
89	12.0
90	10.0
91	9.5
92	6.0
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.188336560035591E-5
2	0.0038624140284628183
3	0.004759632018077411
4	2.188336560035591E-5
5	2.188336560035591E-5
6	2.188336560035591E-5
7	2.188336560035591E-5
8	0.0
9	0.0028229541624459125
10-11	0.0010558723902171726
12-13	1.0941682800177956E-5
14-15	7.330927476119231E-4
16-17	6.0179255400978755E-5
18-19	4.92375726008008E-5
20-21	1.094201263988474E-5
22-23	5.744977494461022E-4
24-25	0.003912803903785301
26-27	0.02116926378369797
28-29	0.3354906511056094
30-31	0.32830175780987464
32-33	0.33855391205565555
34-35	0.17977298716999274
36-37	0.04031216209821416
38-39	0.03712220267580956
40-41	0.013127459846678494
42-43	0.010551554765615688
44-45	0.009144677429416787
46-47	0.00577258138014557
48-49	0.009086799084861511
50-51	0.009653301181865396
52-53	0.002934549503655228
54-55	0.003388867642672694
56-57	0.0035417666049466262
58-59	0.004592584961270235
60-61	0.00292399238657639
62-63	0.001680787608193567
64-65	4.6807416782664637E-4
66-67	0.00659939266120282
68-69	0.01642484800938398
70-71	0.013438192927612112
72-73	0.014560965016170282
74-75	0.007989407627007552
76-77	0.004817870683381135
78-79	0.0013006472748083083
80-81	0.0012547771030259197
82-83	5.965716536463106E-4
84-85	1.0929976305268699E-4
86-87	0.0
88-89	4.728539784882963E-4
90-91	0.004777454566101808
92-93	0.01914026707316874
94-95	0.0016479346150250437
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	176.0
20-21	533.0
22-23	1224.0
24-25	2955.0
26-27	5153.0
28-29	10146.0
30-31	24988.0
32-33	31517.0
34-35	45239.0
36-37	64614.0
38-39	74905.0
40-41	76630.0
42-43	88076.0
44-45	103289.0
46-47	101628.0
48-49	91160.0
50-51	82534.0
52-53	80498.0
54-55	86080.0
56-57	89584.0
58-59	88135.0
60-61	86239.0
62-63	84240.0
64-65	90625.0
66-67	95163.0
68-69	94744.0
70-71	93104.0
72-73	89259.0
74-75	90451.0
76-77	92143.0
78-79	96814.0
80-81	97409.0
82-83	94667.0
84-85	95752.0
86-87	101235.0
88-89	108349.0
90-91	116914.0
92-93	119540.0
94-95	135093.0
96-97	202572.0
98-99	779979.0
100-101	5226006.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.81091540599775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.37254192082531	80.23506549040574
2	6.290287847625903	11.047118681345076
3	1.388143880140724	3.6568255449117175
4	0.45226889812048576	1.5885658381448717
5	0.2029973520595069	0.8912691654669451
6	0.0987497125543979	0.5202781593288497
7	0.05456605238570728	0.3354046507056409
8	0.0335889031755107	0.2359577868260015
9	0.026202469310858845	0.207077653447567
>10	0.07838163355522257	1.1048000667326654
>50	0.001921797393512537	0.11115123816656351
>100	3.30923360731164E-4	0.053167674722915795
>500	1.8609492131659495E-5	0.01331804979547212
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0941682800177956E-5
2	0.0	2.188336560035591E-5	0.0	0.0	1.0941682800177956E-5
3	0.0	2.188336560035591E-5	0.0	0.0	1.0941682800177956E-5
4	0.0	7.659177960124569E-5	0.0	4.376673120071182E-5	1.0941682800177956E-5
5	0.0	7.659177960124569E-5	0.0	4.376673120071182E-5	1.0941682800177956E-5
6	0.0	7.659177960124569E-5	0.0	4.376673120071182E-5	1.0941682800177956E-5
7	0.0	7.659177960124569E-5	0.0	4.376673120071182E-5	2.188336560035591E-5
8	0.0	7.659177960124569E-5	0.0	4.376673120071182E-5	3.2825048400533864E-5
9	2.188336560035591E-5	9.84751452016016E-5	0.0	5.4708414000889775E-5	5.4708414000889775E-5
10-11	2.188336560035591E-5	9.84751452016016E-5	0.0	9.300430380151261E-5	5.4708414000889775E-5
12-13	2.188336560035591E-5	9.84751452016016E-5	0.0	1.0941682800177955E-4	6.017925540097875E-5
14-15	2.188336560035591E-5	1.6412524200266933E-4	0.0	1.3130019360213546E-4	7.659177960124569E-5
16-17	4.376673120071182E-5	1.8600860760302525E-4	0.0	1.5318355920249137E-4	8.753346240142365E-5
18-19	4.376673120071182E-5	2.0789197320338114E-4	0.0	1.750669248028473E-4	1.0394598660169057E-4
20-21	4.376673120071182E-5	2.51658704404093E-4	0.0	1.8600860760302525E-4	1.258293522020465E-4
22-23	4.376673120071182E-5	2.626003872042709E-4	0.0	2.0242113180329218E-4	1.477127178024024E-4
24-25	4.376673120071182E-5	2.626003872042709E-4	0.0	2.243044974036481E-4	1.750669248028473E-4
26-27	4.376673120071182E-5	2.680712286043599E-4	0.0	3.610755324058725E-4	1.750669248028473E-4
28-29	4.376673120071182E-5	2.735420700044489E-4	0.0	8.479804170137915E-4	1.8053776620293626E-4
30-31	4.92375726008008E-5	3.1730880120516073E-4	0.0	0.002188336560035591	1.9147944900311424E-4
32-33	5.4708414000889775E-5	3.1730880120516073E-4	0.0	0.004715865286876699	1.969502904032032E-4
34-35	5.4708414000889775E-5	3.610755324058725E-4	0.0	0.008211732941533555	1.969502904032032E-4
36-37	5.4708414000889775E-5	4.0484226360658435E-4	0.0	0.013359794699017283	1.969502904032032E-4
38-39	5.4708414000889775E-5	4.2672562920694027E-4	0.0	0.020143638035127615	2.0242113180329218E-4
40-41	5.4708414000889775E-5	4.2672562920694027E-4	0.0	0.027201023441242398	2.0789197320338114E-4
42-43	5.4708414000889775E-5	4.650215190075631E-4	0.0	0.034395179882359406	2.0789197320338114E-4
44-45	5.4708414000889775E-5	5.142590916083639E-4	0.0	0.04226772065708744	2.0789197320338114E-4
46-47	5.4708414000889775E-5	5.689675056092537E-4	0.0	0.05121801718763301	2.133628146034701E-4
48-49	5.4708414000889775E-5	5.853800298095207E-4	0.0	0.05945710433616701	2.188336560035591E-4
50-51	5.4708414000889775E-5	6.565009680106773E-4	0.0	0.06738982436629604	2.188336560035591E-4
52-53	5.4708414000889775E-5	6.893260164112112E-4	0.0	0.07551402384542816	2.243044974036481E-4
54-55	5.4708414000889775E-5	7.002676992113892E-4	0.0	0.08347409808255762	2.3524618020382603E-4
56-57	6.565009680106773E-5	7.112093820115671E-4	0.0	0.09100744669048014	2.4071702160391502E-4
58-59	7.659177960124569E-5	7.38563589012012E-4	0.0	0.0983711992149999	2.4071702160391502E-4
60-61	7.659177960124569E-5	7.604469546123679E-4	0.0	0.10555988481471683	2.5712954580418195E-4
62-63	7.659177960124569E-5	7.659177960124569E-4	0.0	0.1119279442044204	2.626003872042709E-4
64-65	7.659177960124569E-5	8.096845272131687E-4	0.0	0.11850389556732735	2.626003872042709E-4
66-67	7.659177960124569E-5	8.096845272131687E-4	0.0	0.12500325515063304	2.626003872042709E-4
68-69	7.659177960124569E-5	8.206262100133466E-4	0.0	0.13161750240334064	2.680712286043599E-4
70-71	7.659177960124569E-5	8.315678928135246E-4	0.0	0.1376354279434385	2.8448375280462683E-4
72-73	7.659177960124569E-5	8.425095756137026E-4	0.0	0.14276707717672196	3.0636711840498275E-4
74-75	7.659177960124569E-5	8.425095756137026E-4	0.0	0.1470179209445911	3.0636711840498275E-4
76-77	7.659177960124569E-5	9.191013552149482E-4	0.0	0.1509405142284549	3.0636711840498275E-4
78-79	8.206262100133467E-5	9.409847208153042E-4	0.0	0.1547153947945163	3.0636711840498275E-4
80-81	9.300430380151263E-5	9.409847208153042E-4	0.0	0.15637305973874327	3.2277964260524965E-4
82-83	9.84751452016016E-5	9.464555622153932E-4	0.0	0.1569365564029524	3.501338496056946E-4
84-85	9.84751452016016E-5	9.628680864156601E-4	0.0	0.15733592782515893	3.501338496056946E-4
86-87	9.84751452016016E-5	9.73809769215838E-4	0.0	0.15741799044616023	3.501338496056946E-4
88-89	9.84751452016016E-5	9.73809769215838E-4	0.0	0.1574398738117606	3.501338496056946E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11015	0.0	46.809467	1
GTATCAA	19760	0.0	34.889683	1
TCAACGC	24440	0.0	27.713335	4
ATCAACG	24655	0.0	27.45521	3
CAACGCA	25025	0.0	27.03218	5
TATCAAC	25685	0.0	26.54925	2
AACGCAG	25715	0.0	26.339254	6
ACGCAGA	29825	0.0	22.723577	7
CGCAGAG	30580	0.0	22.162546	8
GTACATG	26575	0.0	22.131062	1
TACATGG	27015	0.0	21.307732	2
ACATGGG	26425	0.0	21.26271	3
GCAGAGT	35375	0.0	19.09997	9
AGAGTAC	32130	0.0	18.16803	10-11
CATGGGG	17920	0.0	18.02604	4
GAGTACT	21075	0.0	17.759905	12-13
GTGGTAT	5930	0.0	17.6428	1
CAGAGTA	34680	0.0	16.8682	10-11
AGTACTT	22985	0.0	16.329433	12-13
TGGTATC	5880	0.0	16.304182	2
>>END_MODULE