FastQCFastQC Report
Fri 27 May 2016
SRR2139654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17762898
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA252150.054.8197021
GTATCAA487300.039.2626881
ATCAACG669000.028.1298033
TCAACGC670650.028.0601234
TATCAAC677700.027.98112
CAACGCA688300.027.3282345
AACGCAG709550.026.5337416
GTGGTAT110900.023.0208321
ACGCAGA829650.022.7080827
CGCAGAG842800.022.323538
TGGTATC110550.022.0562272
GCAGAGT942700.019.822959
GTACATG615550.019.6886751
AGAGTAC876150.019.62097510-11
TACATGG622800.019.173012
GAGTACT555900.019.01524512-13
AGTACTT602750.018.50977512-13
ACATGGG633850.018.3835093
GTACTTT602500.017.53798914-15
CAGAGTA935350.017.5126069
Produced by FastQC (version 0.11.5)