##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17762898
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10281441688175	33.0	31.0	34.0	30.0	34.0
2	32.325043300929835	34.0	31.0	34.0	31.0	34.0
3	32.39090434455009	34.0	31.0	34.0	31.0	34.0
4	36.045073838739604	37.0	35.0	37.0	35.0	37.0
5	35.92630493064814	37.0	35.0	37.0	35.0	37.0
6	35.9494735600013	37.0	35.0	37.0	35.0	37.0
7	35.88227788055755	37.0	35.0	37.0	35.0	37.0
8	35.84517565771081	37.0	35.0	37.0	35.0	37.0
9	37.47678098472445	39.0	37.0	39.0	35.0	39.0
10-11	37.36069812482175	39.0	37.0	39.0	34.0	39.0
12-13	37.31731950495916	39.0	37.0	39.0	34.0	39.0
14-15	38.52050892821656	40.0	38.0	41.0	34.0	41.0
16-17	38.46687491534321	40.0	38.0	41.0	34.0	41.0
18-19	38.34749329754638	40.0	38.0	41.0	33.5	41.0
20-21	38.388040021252316	40.0	38.0	41.0	34.0	41.0
22-23	38.26608795192933	40.0	38.0	41.0	34.0	41.0
24-25	38.11362286678745	40.0	37.5	41.0	33.0	41.0
26-27	38.03959525886965	40.0	37.5	41.0	33.5	41.0
28-29	37.9518012086956	40.0	37.5	41.0	33.0	41.0
30-31	37.85346484326598	40.0	37.0	41.0	33.0	41.0
32-33	37.68136171330728	40.0	37.0	41.0	33.0	41.0
34-35	37.396546775163294	39.0	36.0	41.0	32.0	41.0
36-37	37.674528884420845	40.0	37.0	41.0	33.0	41.0
38-39	37.7668238063757	40.0	37.0	41.0	33.0	41.0
40-41	37.75285334834082	40.0	37.0	41.0	33.0	41.0
42-43	37.65964889476715	40.0	37.0	41.0	33.0	41.0
44-45	37.54799053296628	40.0	37.0	41.0	32.0	41.0
46-47	37.44789394227133	39.5	36.0	41.0	32.0	41.0
48-49	37.363208347304045	39.0	36.0	41.0	32.0	41.0
50-51	37.27364855036387	39.0	36.0	40.0	31.0	41.0
52-53	37.15132932800575	39.0	36.0	40.0	31.0	41.0
54-55	37.00449099849553	39.0	35.5	40.0	31.0	41.0
56-57	36.83533928958124	39.0	35.0	40.0	31.0	41.0
58-59	36.650355160409404	38.0	35.0	40.0	31.0	41.0
60-61	36.48422555655026	38.0	35.0	40.0	30.5	41.0
62-63	36.245649884646895	38.0	34.5	40.0	30.0	41.0
64-65	35.92810895713302	37.5	34.0	40.0	29.5	41.0
66-67	35.56092425623085	37.0	34.0	39.5	29.0	41.0
68-69	35.22106055758913	36.5	34.0	39.0	29.0	40.0
70-71	34.87117453153259	36.0	33.5	39.0	28.0	40.0
72-73	34.555785503234716	35.5	33.0	38.5	28.0	40.0
74-75	34.22297677005817	35.0	33.0	38.0	28.0	40.0
76-77	33.37618936004787	34.5	31.5	36.5	27.0	39.0
78-79	33.72294658734555	35.0	32.0	37.0	28.0	39.0
80-81	33.55105940801961	35.0	32.0	36.0	28.0	39.0
82-83	33.303976078625	35.0	32.0	36.0	28.0	37.5
84-85	33.0319878820782	34.0	32.0	35.5	27.0	37.0
86-87	32.80819088332012	34.0	32.0	35.0	27.0	37.0
88-89	32.584579622321655	34.0	31.5	35.0	27.0	36.0
90-91	32.37364964490099	34.0	31.0	35.0	26.0	36.0
92-93	32.23202943670559	34.0	31.0	35.0	26.0	35.5
94-95	32.184849421009616	34.0	31.0	35.0	26.0	35.0
96-97	32.20914631421593	34.0	31.0	35.0	26.5	35.0
98-99	32.40418101706912	34.0	31.0	35.0	27.5	35.0
100-101	30.892837973668474	32.5	28.0	34.5	24.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	24.0
20	124.0
21	892.0
22	3962.0
23	12697.0
24	32347.0
25	65058.0
26	114419.0
27	177772.0
28	251384.0
29	334910.0
30	427821.0
31	538003.0
32	679957.0
33	885628.0
34	1219878.0
35	1777713.0
36	2806044.0
37	4072070.0
38	3302194.0
39	1046883.0
40	13114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.345617085503235	24.47616224720126	18.11424883162345	25.063971835672056
2	11.792961374473034	23.776458969575344	40.57468930897235	23.855890346979272
3	14.622287701770869	28.24506458598889	32.91818413719088	24.214463575049365
4	10.946047927435252	20.52718320971891	38.956127365499825	29.570641497346013
5	12.259904705848907	38.68176893462468	35.97574607067148	13.082580288854942
6	28.763875483134925	40.012762559256245	18.347352726005532	12.876009231603295
7	25.154491990185157	33.584660608532566	23.59528106201157	17.66556633927071
8	24.30153570661724	37.54498843600859	20.801442422289426	17.35203343508475
9	25.072820889184165	16.524693683297716	21.51666071120839	36.88582471630972
10-11	23.523505146478914	27.72212847723213	29.058488598062677	19.695877778226283
12-13	24.460352696952942	25.247304803529243	29.376692924769372	20.915649574748446
14-15	21.595375722543352	26.466605020893414	27.220271044361034	24.717748212202203
16-17	20.884402729613587	29.717775785295636	27.794889460636746	21.60293202445403
18-19	21.024372636628446	28.502349969316647	30.082134994744518	20.391142399310382
20-21	21.88986282184935	27.524352092932467	29.98798123951884	20.59780384569934
22-23	21.497823852611383	27.554806831376478	29.88348836274371	21.06388095326843
24-25	21.27793869635369	27.748111021042515	29.939044738661853	21.03490554394194
26-27	21.22734728931546	27.852854619005146	29.9014893560254	21.01830873565399
28-29	21.157428382013563	27.797824809986405	30.016784732232544	21.02796207576749
30-31	21.33576820570479	27.746119896200767	29.869975868251085	21.048136029843356
32-33	21.298718886524128	27.884225086456865	29.76503916713319	21.05201685988582
34-35	21.359516986808703	27.919252193439643	29.729478616092536	20.991752203659118
36-37	21.355605904740788	27.802061724013672	29.72578961350386	21.11654275774168
38-39	21.25188841028668	27.921537642812144	29.772578638365747	21.053995308535427
40-41	21.404631573861383	27.850509354633402	29.66395047291567	21.080908598589552
42-43	21.4139833677158	27.990181578475926	29.32416771246637	21.2716673413419
44-45	21.550786513111085	28.06952838433817	29.026002430559718	21.353682671991027
46-47	21.671977494521816	28.09568276728429	28.686755084869414	21.545584653324475
48-49	21.616980267810927	28.2355880313277	28.58071193522791	21.56671976563346
50-51	21.63896901170482	28.310663279214616	28.427664287951828	21.62270342112874
52-53	21.797280702872936	28.274765081493836	28.347660458802604	21.580293756830628
54-55	21.795214378109822	28.248959846754058	28.316167894460815	21.63965788067531
56-57	21.809699517506537	28.41110708673447	28.18101412999214	21.598179265766856
58-59	21.695565103963997	28.477158976532092	28.243670228142747	21.583605691361164
60-61	21.830483089586895	28.493950207571505	28.16185771682147	21.513708986020134
62-63	21.812845480041037	28.551046342008558	28.134101738072125	21.50200643987828
64-65	21.775845716868737	28.44201505514461	28.15867182574174	21.623467402244913
66-67	21.86282334873987	28.45136560303738	28.019238939753322	21.666572108469428
68-69	21.82644929780892	28.37592654772133	28.135057625877153	21.6625665285926
70-71	21.797802920831728	28.209311328477593	28.206234362117367	21.786651388573315
72-73	21.871888044446006	28.2840829223189	28.090399254437763	21.753629778797333
74-75	21.752659306255755	28.361201804597286	28.058671762646476	21.827467126500483
76-77	21.84135275218635	28.265110854890512	28.10700150696377	21.78653488595937
78-79	21.812777248532164	28.261370314449017	28.05713828074612	21.868714156272695
80-81	21.789666835875778	28.303028585042604	28.013709947553544	21.893594631528075
82-83	21.84664057770412	28.251010218104383	28.09890565754992	21.803443546641578
84-85	21.851227873305447	28.237115650537138	28.0917691445815	21.819887331575917
86-87	21.8079863947619	28.212887720576024	28.13239843930259	21.846727445359484
88-89	21.809220274170006	28.18694313358313	28.134692401078205	21.86914419116866
90-91	21.811033803568677	28.219255216379675	28.190567477456142	21.779143502595506
92-93	21.844771657275317	28.124462831643594	28.190092595879573	21.840672915201516
94-95	21.61359731868577	28.113800907725288	28.318789265671096	21.953812507917846
96-97	21.58496766913759	28.16115982427702	28.390494663940313	21.863377842645075
98-99	21.608174002986154	28.711850723429677	28.478566817970957	21.201408455613212
100-101	19.72929024501476	32.475784933490495	26.540049604499515	21.25487521699523
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7141.0
1	5966.5
2	6556.0
3	8540.0
4	10126.0
5	13032.0
6	15899.5
7	21418.0
8	27051.5
9	28906.0
10	27401.5
11	25755.0
12	29520.5
13	41978.5
14	73391.0
15	116874.5
16	148546.0
17	159322.5
18	153764.5
19	139178.5
20	121147.5
21	104661.0
22	95278.0
23	98405.5
24	114358.5
25	142315.0
26	179073.5
27	218326.5
28	260215.5
29	303871.0
30	344240.0
31	385402.0
32	431064.0
33	479352.5
34	523029.0
35	560738.0
36	602647.0
37	636487.5
38	657209.5
39	675963.5
40	693165.5
41	706012.5
42	710258.5
43	716453.5
44	720475.0
45	718275.0
46	711291.0
47	699349.5
48	693606.5
49	682796.0
50	660977.5
51	634547.0
52	600528.5
53	558948.5
54	517004.0
55	474738.0
56	429747.0
57	383305.0
58	334957.0
59	286327.5
60	239292.5
61	192938.0
62	150046.0
63	114017.5
64	83663.5
65	60435.0
66	44256.0
67	33130.0
68	24903.5
69	17888.0
70	12442.5
71	8709.5
72	6082.5
73	4068.0
74	2745.0
75	1823.5
76	1181.5
77	770.5
78	517.5
79	358.5
80	253.0
81	174.0
82	133.0
83	97.5
84	71.5
85	56.5
86	42.5
87	30.5
88	24.0
89	19.0
90	14.5
91	11.5
92	9.0
93	6.0
94	2.0
95	0.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.6889135995714212E-5
2	0.002229365951434276
3	0.0034284946071299854
4	1.6889135995714212E-5
5	1.6889135995714212E-5
6	1.6889135995714212E-5
7	1.6889135995714212E-5
8	0.0
9	0.0027078914713128456
10-11	9.626807517557102E-4
12-13	0.0
14-15	6.924545758242827E-4
16-17	9.851995997499958E-5
18-19	3.659312799071413E-5
20-21	5.629923086242765E-6
22-23	6.41877435192901E-4
24-25	0.0031903296013141118
26-27	0.018425107136603704
28-29	0.3084995224854355
30-31	0.3033642513679155
32-33	0.311843221555309
34-35	0.16329841080567908
36-37	0.030503156435223007
38-39	0.02829062476254143
40-41	0.006901932454813193
42-43	0.005997289781020735
44-45	0.006696886023967021
46-47	0.004472811549998077
48-49	0.005602075874706458
50-51	0.005576892388404729
52-53	0.0015022879451660053
54-55	0.0014890823563544345
56-57	0.0014120840181992096
58-59	0.0018680240395455103
60-61	0.0010800708150777885
62-63	5.338894376261393E-4
64-65	1.3076381376171133E-4
66-67	0.005660277129231218
68-69	0.014461124193718197
70-71	0.01138743192493057
72-73	0.012410066145286279
74-75	0.006105701062048665
76-77	0.0034885354981630395
78-79	0.0010707984751416543
80-81	7.810126617145118E-4
82-83	3.0027286678560296E-4
84-85	5.3702064597354276E-5
86-87	0.0
88-89	2.620464990071021E-4
90-91	0.003540735485183375
92-93	0.014209255870134078
94-95	0.0010376860450211883
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	394.0
20-21	1492.0
22-23	2960.0
24-25	5832.0
26-27	9946.0
28-29	14393.0
30-31	30539.0
32-33	41040.0
34-35	60696.0
36-37	81255.0
38-39	100777.0
40-41	113895.0
42-43	144632.0
44-45	175028.0
46-47	171476.0
48-49	145427.0
50-51	125138.0
52-53	120309.0
54-55	130317.0
56-57	138779.0
58-59	141307.0
60-61	144344.0
62-63	146510.0
64-65	161573.0
66-67	168748.0
68-69	165821.0
70-71	164292.0
72-73	160528.0
74-75	163755.0
76-77	165830.0
78-79	178961.0
80-81	186602.0
82-83	186790.0
84-85	194418.0
86-87	214237.0
88-89	238421.0
90-91	266092.0
92-93	294848.0
94-95	372775.0
96-97	550928.0
98-99	1758580.0
100-101	1.0123213E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.8000518885177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.70903929169779	74.3382209562483
2	7.869181926571252	13.188757075337133
3	1.9520416818987072	4.907435826949832
4	0.6858682013742259	2.299031634553778
5	0.31266696017001094	1.3100753743036007
6	0.1614626084364753	0.8118344979019229
7	0.08841852923880111	0.5186634136682567
8	0.06039073363107375	0.4048597289495705
9	0.03596662826128589	0.2712604783095706
>10	0.12075061804878247	1.5658208608995745
>50	0.0030651588411997983	0.18059831183405228
>100	0.001075689137526706	0.14296862761406612
>500	4.799441440975782E-5	0.03051334426356639
>1k	2.3978278458758828E-5	0.029959869166678244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.629711998571404E-6
2	5.629711998571404E-6	1.6889135995714212E-5	0.0	1.1259423997142809E-5	5.629711998571404E-6
3	5.629711998571404E-6	1.6889135995714212E-5	0.0	1.1259423997142809E-5	5.629711998571404E-6
4	1.1259423997142809E-5	5.066740798714264E-5	0.0	2.2518847994285618E-5	5.629711998571404E-6
5	1.1259423997142809E-5	5.066740798714264E-5	0.0	2.2518847994285618E-5	5.629711998571404E-6
6	7.318625598142826E-5	5.066740798714264E-5	0.0	2.2518847994285618E-5	5.629711998571404E-6
7	7.318625598142826E-5	6.755654398285685E-5	0.0	2.2518847994285618E-5	1.1259423997142809E-5
8	7.318625598142826E-5	6.755654398285685E-5	0.0	2.2518847994285618E-5	1.6889135995714212E-5
9	7.881596797999966E-5	9.007539197714247E-5	0.0	2.8148559992857023E-5	2.8148559992857023E-5
10-11	8.163082397928536E-5	9.007539197714247E-5	0.0	7.037139998214255E-5	2.8148559992857023E-5
12-13	9.007539197714247E-5	1.0696452797285668E-4	0.0	8.726053597785677E-5	2.8148559992857023E-5
14-15	1.0133481597428528E-4	1.351130879657137E-4	0.0	1.0133481597428528E-4	4.222283998928553E-5
16-17	1.407427999642851E-4	1.407427999642851E-4	0.0	1.0977938397214239E-4	5.066740798714264E-5
18-19	1.4637251196285652E-4	1.548170799607136E-4	0.0	1.1259423997142809E-4	6.474168798357115E-5
20-21	1.5200222396142792E-4	1.8859535195214202E-4	0.0	1.1822395196999949E-4	8.444567997857106E-5
22-23	1.60446791959285E-4	2.3363304794071327E-4	0.0	1.2666851996785661E-4	9.007539197714247E-5
24-25	1.8015078395428494E-4	2.730410319307131E-4	0.0	1.5200222396142792E-4	9.570510397571387E-5
26-27	1.8578049595285634E-4	2.9555987992499873E-4	0.0	1.9703991994999914E-4	9.570510397571387E-5
28-29	1.8578049595285634E-4	3.068193039221415E-4	0.0	4.897849438757122E-4	9.851995997499957E-5
30-31	1.9703991994999914E-4	3.5185699991071277E-4	0.0	0.0012075732236935663	1.0133481597428528E-4
32-33	2.1674391194499904E-4	3.603015679085699E-4	0.0	0.0028317451352814164	1.0133481597428528E-4
34-35	2.3081819194142758E-4	4.081541198964268E-4	0.0	0.005303188702654263	1.0696452797285668E-4
36-37	2.3644790393999898E-4	4.6163638388285516E-4	0.0	0.008624718781811392	1.0696452797285668E-4
38-39	2.3644790393999898E-4	4.925997998749979E-4	0.0	0.014153095964408509	1.2103880796928519E-4
40-41	2.3644790393999898E-4	5.235632158671407E-4	0.0	0.019965773602933487	1.238536639685709E-4
42-43	2.3644790393999898E-4	5.545266318592833E-4	0.0	0.02515073835361775	1.238536639685709E-4
44-45	2.505221839364275E-4	6.446020238364258E-4	0.0	0.031357495832042726	1.2666851996785661E-4
46-47	2.730410319307131E-4	6.924545758242827E-4	0.0	0.03846500723023912	1.407427999642851E-4
48-49	2.758558879299988E-4	7.065288558207113E-4	0.0	0.045564074060437665	1.491873679621422E-4
50-51	2.9274502392571304E-4	7.909745357992823E-4	0.0	0.05270254887462621	1.6326164795857072E-4
52-53	3.0400444792285584E-4	8.022339597964251E-4	0.0	0.05909227199300475	1.6607650395785643E-4
54-55	3.068193039221415E-4	8.163082397928536E-4	0.0	0.06596615034326042	1.801507839542849E-4
56-57	3.096341599214272E-4	8.21937951791425E-4	0.0	0.07291321494949754	2.0829934394714196E-4
58-59	3.1526387191999864E-4	8.275676637899965E-4	0.0	0.08018117313965323	2.2237362394357047E-4
60-61	3.2089358391857006E-4	8.472716557849963E-4	0.0	0.08674823218598676	2.3363304794071327E-4
62-63	3.5467185590999845E-4	8.585310797821391E-4	0.0	0.09332936551231674	2.477073279371418E-4
64-65	3.5467185590999845E-4	9.289024797642817E-4	0.0	0.09987390571065599	2.477073279371418E-4
66-67	3.6311642390785557E-4	9.317173357635674E-4	0.0	0.10659296698095097	2.477073279371418E-4
68-69	3.6593127990714125E-4	9.514213277585673E-4	0.0	0.11349499389119952	2.505221839364275E-4
70-71	3.6874613590642694E-4	9.711253197535671E-4	0.0	0.11967641766563092	2.533370399357132E-4
72-73	4.3348782388999814E-4	9.851995997499957E-4	0.0	0.1241661129844916	2.64596463932856E-4
74-75	4.3348782388999814E-4	0.0010189778717414242	0.0	0.12774098010358445	2.64596463932856E-4
76-77	4.391175358885695E-4	0.0010668304237292812	0.0	0.13078102458281302	2.64596463932856E-4
78-79	4.391175358885695E-4	0.001086534415724281	0.0	0.13373943823806228	2.64596463932856E-4
80-81	4.475621038864267E-4	0.0010921641277228523	0.0	0.13534109130165584	2.7022617593142745E-4
82-83	4.5037695988571236E-4	0.0011034235517199953	0.0	0.13596598933349727	2.814855999285702E-4
84-85	4.5037695988571236E-4	0.0011118681197178522	0.0	0.1365514793813487	2.814855999285702E-4
86-87	4.6163638388285516E-4	0.0011372018237114237	0.0	0.13675696386929656	2.8430045592785593E-4
88-89	4.6163638388285516E-4	0.001154090959707138	0.0	0.1368639283972694	2.9555987992499873E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25215	0.0	54.819702	1
GTATCAA	48730	0.0	39.262688	1
ATCAACG	66900	0.0	28.129803	3
TCAACGC	67065	0.0	28.060123	4
TATCAAC	67770	0.0	27.9811	2
CAACGCA	68830	0.0	27.328234	5
AACGCAG	70955	0.0	26.533741	6
GTGGTAT	11090	0.0	23.020832	1
ACGCAGA	82965	0.0	22.708082	7
CGCAGAG	84280	0.0	22.32353	8
TGGTATC	11055	0.0	22.056227	2
GCAGAGT	94270	0.0	19.82295	9
GTACATG	61555	0.0	19.688675	1
AGAGTAC	87615	0.0	19.620975	10-11
TACATGG	62280	0.0	19.17301	2
GAGTACT	55590	0.0	19.015245	12-13
AGTACTT	60275	0.0	18.509775	12-13
ACATGGG	63385	0.0	18.383509	3
GTACTTT	60250	0.0	17.537989	14-15
CAGAGTA	93535	0.0	17.512606	9
>>END_MODULE