FastQCFastQC Report
Fri 27 May 2016
SRR2139653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8479972
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105900.050.933771
GTATCAA190100.036.3141561
CTTATAC71000.030.5682561
TCAACGC237900.028.3902634
ATCAACG240250.028.130813
CAACGCA245300.027.533815
TATCAAC252500.026.9852622
AACGCAG253650.026.7114666
ACGCAGA289750.023.3246177
TATACAC110800.023.013243
CGCAGAG296550.022.8041538
GTGGTAT55000.021.319711
TGGTATC53650.020.981932
TTATACA113250.020.6702332
GTACATG243600.019.9369031
GCAGAGT342550.019.6922959
ACATCTC113050.019.4997318
TACATGG240750.019.4821832
ACACATC119350.018.934866
AGAGTAC313000.018.85962110-11
Produced by FastQC (version 0.11.5)