##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139653_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8479972
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57601322268517	34.0	31.0	34.0	31.0	34.0
2	32.71593149128323	34.0	31.0	34.0	31.0	34.0
3	32.78577724077391	34.0	31.0	34.0	31.0	34.0
4	36.271338395928666	37.0	37.0	37.0	35.0	37.0
5	36.20101222032337	37.0	37.0	37.0	35.0	37.0
6	36.22739756687876	37.0	37.0	37.0	35.0	37.0
7	36.175441145324534	37.0	37.0	37.0	35.0	37.0
8	36.15252656494621	37.0	37.0	37.0	35.0	37.0
9	37.87356656366318	39.0	38.0	39.0	35.0	39.0
10-11	37.80720508275263	39.0	38.0	39.0	35.0	39.0
12-13	37.77454890181241	39.0	38.0	39.0	35.0	39.0
14-15	39.14913693111251	40.0	39.0	41.0	36.0	41.0
16-17	39.108259378686625	40.0	38.0	41.0	36.0	41.0
18-19	38.991912591220824	40.0	38.5	41.0	35.5	41.0
20-21	39.05493171553748	40.0	39.0	41.0	36.0	41.0
22-23	38.96852398330447	40.0	38.0	41.0	35.0	41.0
24-25	38.85597017526797	40.0	38.0	41.0	35.0	41.0
26-27	38.80606721040051	40.0	38.0	41.0	35.0	41.0
28-29	38.700301230039194	40.0	38.0	41.0	35.0	41.0
30-31	38.63417430678915	40.0	38.0	41.0	35.0	41.0
32-33	38.500522672414014	40.0	38.0	41.0	34.0	41.0
34-35	38.265591253378275	40.0	38.0	41.0	34.0	41.0
36-37	38.535927528385216	40.0	38.0	41.0	34.0	41.0
38-39	38.62484567649719	40.0	38.0	41.0	35.0	41.0
40-41	38.62231987629961	40.0	38.0	41.0	34.5	41.0
42-43	38.55119008780672	40.0	38.0	41.0	34.0	41.0
44-45	38.47947330376238	40.0	38.0	41.0	34.0	41.0
46-47	38.40950866032111	40.0	38.0	41.0	34.0	41.0
48-49	38.340763704385466	40.0	38.0	41.0	34.0	41.0
50-51	38.25681903903654	40.0	38.0	41.0	33.0	41.0
52-53	38.16178118156655	40.0	37.5	41.0	33.0	41.0
54-55	38.03639236321685	40.0	37.0	41.0	33.0	41.0
56-57	37.88293535260836	40.0	37.0	41.0	33.0	41.0
58-59	37.71746769139304	39.5	36.0	41.0	33.0	41.0
60-61	37.54159684970742	39.0	36.0	41.0	33.0	41.0
62-63	37.325644807609095	39.0	35.5	41.0	32.0	41.0
64-65	37.04425873493562	38.5	35.0	40.0	32.0	41.0
66-67	36.723833461388764	38.0	35.0	40.0	31.0	41.0
68-69	36.409921329470464	37.5	35.0	40.0	31.0	41.0
70-71	36.07474509156437	37.0	35.0	39.5	31.0	41.0
72-73	35.75397267615138	36.5	34.5	39.0	31.0	41.0
74-75	35.41986439954664	36.0	34.0	39.0	30.5	40.0
76-77	34.50022533175686	35.0	33.5	37.0	29.5	39.0
78-79	34.74837172414493	35.0	34.0	37.0	30.0	39.0
80-81	34.550001240978716	35.0	34.0	37.0	30.0	39.0
82-83	34.29674670879998	35.0	34.0	36.0	30.0	38.5
84-85	34.03273171084332	35.0	34.0	36.0	30.0	37.0
86-87	33.823934269958016	35.0	34.0	35.5	30.0	37.0
88-89	33.61657149149205	35.0	33.0	35.0	29.5	36.5
90-91	33.43297647283535	35.0	33.0	35.0	29.0	36.0
92-93	33.29688591712883	35.0	33.0	35.0	29.0	36.0
94-95	33.24287474622599	35.0	33.0	35.0	29.0	36.0
96-97	33.235741215224365	35.0	33.0	35.0	29.0	35.0
98-99	33.32361875572033	35.0	33.0	35.0	29.5	35.0
100-101	31.736904079232453	33.0	30.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	45.0
21	221.0
22	1074.0
23	3713.0
24	9680.0
25	19868.0
26	35428.0
27	55975.0
28	80202.0
29	107933.0
30	138441.0
31	175785.0
32	221320.0
33	286211.0
34	395374.0
35	568623.0
36	949798.0
37	1833412.0
38	2397720.0
39	1138694.0
40	60447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.129795711589615	21.69271313631696	15.654355934194122	24.523135217899306
2	13.436265825180731	21.758388924575833	41.725998257229726	23.079346993013704
3	16.622291198750876	26.76354304805629	31.573299715137683	25.040866038055153
4	11.742338300173632	19.17621897808153	39.11209848334405	29.969344238400787
5	12.750796818668741	38.086729531654115	36.017465623707245	13.145008025969899
6	29.13575658032833	38.84225089422465	18.82032157653351	13.20167094891351
7	25.76522658329532	33.12112351314367	23.27103202699254	17.842617876568458
8	23.99129383917777	37.27644383731455	21.241956930989865	17.490305392517804
9	25.025366379676328	17.085229620572736	21.52462909187653	36.364774907874406
10-11	23.67863153925595	27.2289654615315	29.173577377307552	19.918825621904997
12-13	24.526755512871976	24.70137873096751	29.24769680843286	21.524168947727656
14-15	21.706346085063764	26.191104846377694	26.82268502265528	25.279864045903256
16-17	21.266140316441035	29.427473698752536	27.50967765976961	21.79670832503682
18-19	21.36763243872899	28.405099617999763	29.29413004305382	20.933137900217435
20-21	22.055801801984536	27.483406963544493	29.23215297787839	21.228638256592582
22-23	21.74028699148149	27.59439216602487	29.09432134455359	21.570999497940043
24-25	21.601297000980136	27.730196382075974	29.175373637421743	21.493132979522148
26-27	21.53832128255988	27.84067664893791	29.146762754214674	21.474239314287537
28-29	21.412434859165984	27.750898442186944	29.282078925515144	21.554587773131935
30-31	21.674590342651612	27.615983871814887	29.042512312928903	21.6669134726046
32-33	21.62534694635233	27.782991646556525	29.127514386485988	21.46414702060516
34-35	21.721511082430922	27.977316218398308	28.997937702865656	21.30323499630511
36-37	21.585428320807836	27.80041968325185	28.959181957555817	21.654970038384498
38-39	21.509265269350713	27.81443056423094	29.09901816312481	21.577286003293533
40-41	21.712475010162418	27.752278152100473	28.994955374934577	21.540291462802532
42-43	21.722613800198467	27.800668523406213	28.815769816119623	21.66094786027569
44-45	21.680035560559578	28.006433286408388	28.595793952083643	21.717737200948395
46-47	21.90336875671679	27.82799055403926	28.251965424508562	22.01667526473538
48-49	21.828059967376763	27.940044609271837	28.219406600172064	22.01248882317934
50-51	21.73884960606341	28.100904627312524	28.19938956212039	21.960856204503678
52-53	21.914232675889313	28.071286243455447	28.176561099186966	21.837919981468275
54-55	21.8730023003272	28.04286417841293	28.081322294353917	22.00281122690595
56-57	22.003948730976905	28.19891700056355	27.861630637613015	21.935503630846526
58-59	21.793925407698488	28.335646876222548	28.09422153945304	21.77620617662592
60-61	22.007811599992174	28.46503794079982	27.796464918667695	21.73068554054031
62-63	21.88659494146933	28.591047027690212	27.782248888874744	21.74010914196571
64-65	21.861188295988935	28.43590009911764	27.81148107841544	21.89143052647799
66-67	21.951781386053227	28.3801060398338	27.69253594578098	21.975576628331993
68-69	21.907937564946348	28.249755280590815	27.832434556476116	22.00987259798672
70-71	21.926709310424663	28.06663408945945	27.888227518993798	22.11842908112209
72-73	21.96405337990186	28.11975375381169	27.80480249125966	22.111390375026787
74-75	21.80868334700806	28.22605873713301	27.80512330687043	22.160134608988503
76-77	21.9283973202724	28.184465440663907	27.759468834818207	22.127668404245487
78-79	21.879277673745392	28.166364057938008	27.752010495400338	22.202347772916262
80-81	21.887471806516086	28.20773870640946	27.72654465019237	22.17824483688208
82-83	21.929933131237732	28.15598686287676	27.830846527377815	22.08323347850769
84-85	21.974632188959415	28.12347660338682	27.806431158839462	22.095460048814306
86-87	21.855268650310496	28.16632703987954	27.85495363926435	22.123450670545612
88-89	21.920124570872048	28.156138082768585	27.80356141125782	22.12017593510155
90-91	21.946968834993857	28.194450552310045	27.798443692575887	22.06013692012021
92-93	21.96100479729712	28.143497140347073	27.835423017588674	22.06007504476713
94-95	21.762166463202824	28.210184623903068	27.91906372711833	22.108585185775777
96-97	21.740251598181217	28.246163063044328	27.968699510485607	22.04488582828885
98-99	21.90316116534628	28.764875358746888	27.99975117356149	21.332212302345344
100-101	20.241512285658395	32.455385222500475	25.94466678176571	21.35843571007542
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2892.0
1	2357.0
2	2511.0
3	3354.0
4	4261.5
5	5993.5
6	7388.5
7	8860.5
8	10182.5
9	10462.0
10	9867.5
11	9463.5
12	11024.5
13	15169.5
14	24653.5
15	37696.0
16	47916.5
17	53025.0
18	53604.5
19	50845.5
20	46239.5
21	41765.0
22	39926.0
23	42744.0
24	50123.0
25	61858.5
26	77560.0
27	95061.5
28	114093.5
29	134033.0
30	152984.0
31	172594.5
32	193856.5
33	215505.5
34	237170.5
35	256327.5
36	276558.0
37	294019.0
38	304810.0
39	316100.0
40	327568.0
41	336866.0
42	341328.0
43	344826.5
44	348911.5
45	350832.5
46	351719.0
47	351370.0
48	355910.0
49	353095.0
50	339019.5
51	324920.5
52	306643.0
53	284778.0
54	262541.5
55	240196.0
56	218182.0
57	196140.5
58	172870.0
59	147976.0
60	122524.5
61	98827.5
62	77666.5
63	59426.5
64	43675.0
65	31732.5
66	23484.5
67	17648.0
68	13308.5
69	9783.5
70	7097.5
71	5123.5
72	3585.0
73	2402.0
74	1588.5
75	1017.5
76	651.0
77	420.0
78	276.0
79	193.5
80	133.5
81	84.5
82	59.5
83	38.5
84	24.0
85	17.0
86	13.0
87	11.5
88	10.0
89	9.0
90	7.5
91	5.5
92	4.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004351429462267092
3	0.005153318902468075
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0028773679913094053
10-11	9.37503095529089E-4
12-13	0.0
14-15	6.014170801507363E-4
16-17	4.127372118681524E-5
18-19	4.127372118681524E-5
20-21	0.0
22-23	6.191680633820529E-4
24-25	0.00392793547711323
26-27	0.020636507042871725
28-29	0.3309936426200105
30-31	0.32427242854753147
32-33	0.3347184568837965
34-35	0.17951186451303883
36-37	0.04027050621742998
38-39	0.03696754419833111
40-41	0.014541046340161574
42-43	0.011610359258034891
44-45	0.010153179512867838
46-47	0.006517698705711185
48-49	0.010149642306255906
50-51	0.01036112290148706
52-53	0.0034251054393338344
54-55	0.004084729433978595
56-57	0.004294157251006903
58-59	0.005277098077568136
60-61	0.003802670683267678
62-63	0.0022609210276197693
64-65	7.768094520566936E-4
66-67	0.006608600362091714
68-69	0.015399166034848949
70-71	0.012508968335290166
72-73	0.014159233651396593
74-75	0.006966283681046407
76-77	0.0040927832537569825
78-79	0.0013259599391470863
80-81	0.001297198626689337
82-83	5.454973944759248E-4
84-85	8.947442276694582E-5
86-87	0.0
88-89	4.5109792647868367E-4
90-91	0.0044750231661944495
92-93	0.01691061162539569
94-95	0.0016105903446631286
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	120.0
20-21	511.0
22-23	1127.0
24-25	2218.0
26-27	3887.0
28-29	6706.0
30-31	15390.0
32-33	20865.0
34-35	30940.0
36-37	42963.0
38-39	51780.0
40-41	54132.0
42-43	65472.0
44-45	78233.0
46-47	77826.0
48-49	68974.0
50-51	61218.0
52-53	59890.0
54-55	65158.0
56-57	70576.0
58-59	70786.0
60-61	72233.0
62-63	73276.0
64-65	78483.0
66-67	85451.0
68-69	76508.0
70-71	73239.0
72-73	70468.0
74-75	71243.0
76-77	72921.0
78-79	76620.0
80-81	78732.0
82-83	76676.0
84-85	79306.0
86-87	84159.0
88-89	90991.0
90-91	97502.0
92-93	104792.0
94-95	123187.0
96-97	195113.0
98-99	768551.0
100-101	5181749.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.02164499970911
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.99173163975411	77.41236792024019
2	7.176077421785025	12.345959689344385
3	1.6529633226463984	4.265718724146845
4	0.5680933536706833	1.9547329918461487
5	0.2487160148482936	1.0697480367511145
6	0.12895302177986587	0.6655650636712428
7	0.06901033097899828	0.4155467534951473
8	0.04354269906803753	0.29964916812479114
9	0.028565145349127928	0.2211498713328997
>10	0.0904582955303669	1.1840177316629994
>50	0.0014662616449933718	0.08136201006536088
>100	4.014412695705001E-4	0.06960429697102903
>500	2.1051674500640388E-5	0.014577742347875304
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	4.716996707064599E-5	0.0	0.0	0.0
5	0.0	5.8962458838307487E-5	0.0	0.0	0.0
6	0.0	5.8962458838307487E-5	0.0	1.1792491767661498E-5	0.0
7	0.0	5.8962458838307487E-5	0.0	1.1792491767661498E-5	1.1792491767661498E-5
8	0.0	7.075495060596898E-5	0.0	1.1792491767661498E-5	1.1792491767661498E-5
9	0.0	1.8867986828258396E-4	0.0	1.1792491767661498E-5	1.1792491767661498E-5
10-11	1.1792491767661498E-5	1.8867986828258396E-4	0.0	5.3066212954476735E-5	1.1792491767661498E-5
12-13	1.1792491767661498E-5	1.8867986828258396E-4	0.0	5.8962458838307487E-5	1.1792491767661498E-5
14-15	1.7688737651492247E-5	2.2405734358556844E-4	0.0	5.8962458838307487E-5	2.3584983535322992E-5
16-17	2.9481229419153743E-5	2.5943481888855295E-4	0.0	5.8962458838307487E-5	3.537747530298449E-5
18-19	3.537747530298449E-5	2.7712355654004516E-4	0.0	8.844368825746123E-5	3.537747530298449E-5
20-21	3.537747530298449E-5	2.9481229419153743E-4	0.0	1.0023618002512273E-4	3.537747530298449E-5
22-23	3.537747530298449E-5	3.3608601537835265E-4	0.0	1.1792491767661497E-4	3.537747530298449E-5
24-25	5.3066212954476735E-5	3.8325598244899866E-4	0.0	1.4150990121193795E-4	3.537747530298449E-5
26-27	7.075495060596898E-5	4.127372118681524E-4	0.0	1.7099113063109172E-4	4.716996707064599E-5
28-29	7.075495060596898E-5	4.1863345775198314E-4	0.0	3.183972777268604E-4	4.716996707064599E-5
30-31	1.0023618002512273E-4	4.716996707064599E-4	0.0	9.021256202261046E-4	4.716996707064599E-5
32-33	1.1792491767661497E-4	4.8349216247412137E-4	0.0	0.002423357058254438	4.716996707064599E-5
34-35	1.238211635604457E-4	5.719358507315826E-4	0.0	0.004227608298706647	4.716996707064599E-5
36-37	1.2971740944427648E-4	6.485870472213824E-4	0.0	0.007116768781783713	4.716996707064599E-5
38-39	1.2971740944427648E-4	6.603795389890438E-4	0.0	0.011320792096955037	4.716996707064599E-5
40-41	1.4150990121193795E-4	7.075495060596898E-4	0.0	0.01623236491818605	5.8962458838307487E-5
42-43	1.4150990121193795E-4	7.429269813626744E-4	0.0	0.020754785511084235	5.8962458838307487E-5
44-45	1.5330239297959945E-4	8.372669155039663E-4	0.0	0.026055510560648077	5.8962458838307487E-5
46-47	1.591986388634302E-4	9.080218661099352E-4	0.0	0.03201661514920096	5.8962458838307487E-5
48-49	1.6509488474726096E-4	9.139181119937659E-4	0.0	0.03780672860712276	5.8962458838307487E-5
50-51	1.6509488474726096E-4	0.0010436355214380425	0.0	0.043885758113352255	5.8962458838307487E-5
52-53	1.7688737651492246E-4	0.0010495317673218732	0.0	0.04950488044064297	8.844368825746123E-5
54-55	1.7688737651492246E-4	0.001055428013205704	0.0	0.055660561143362264	1.238211635604457E-4
56-57	1.7688737651492246E-4	0.0010731167508571961	0.0	0.06209926164850544	1.2971740944427648E-4
58-59	1.7688737651492246E-4	0.0010731167508571961	0.0	0.06850258467834563	1.2971740944427648E-4
60-61	1.7688737651492246E-4	0.0010849092426248577	0.0	0.07437524557864106	1.2971740944427648E-4
62-63	1.7688737651492246E-4	0.0011202867179278422	0.0	0.08014177405302753	1.2971740944427648E-4
64-65	1.7688737651492246E-4	0.0012500041273721186	0.0	0.08605570867450978	1.2971740944427648E-4
66-67	1.8867986828258396E-4	0.0012500041273721186	0.0	0.09175148219829027	1.2971740944427648E-4
68-69	1.8867986828258396E-4	0.0012853816026751031	0.0	0.09779513422921679	1.2971740944427648E-4
70-71	1.8867986828258396E-4	0.0013207590779780877	0.0	0.10349680399888113	1.2971740944427648E-4
72-73	1.8867986828258396E-4	0.0013325515697457492	0.0	0.10782464847761289	1.4150990121193795E-4
74-75	1.8867986828258396E-4	0.0013502403073972413	0.0	0.1112562635820024	1.4150990121193795E-4
76-77	1.8867986828258396E-4	0.0014209952580032104	0.0	0.11491783227586129	1.4150990121193795E-4
78-79	1.8867986828258396E-4	0.0014740614709576872	0.0	0.11810770129901371	1.4150990121193795E-4
80-81	1.8867986828258396E-4	0.0014740614709576872	0.0	0.11945204536052713	1.4150990121193795E-4
82-83	1.8867986828258396E-4	0.0014740614709576872	0.0	0.12002987745714255	1.4150990121193795E-4
84-85	1.8867986828258396E-4	0.0014799577168415178	0.0	0.12048388839019752	1.4150990121193795E-4
86-87	1.8867986828258396E-4	0.0015094389462606717	0.0	0.1205959170619903	1.4150990121193795E-4
88-89	1.8867986828258396E-4	0.0015684014050989792	0.0	0.12066077576671244	1.4150990121193795E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10590	0.0	50.93377	1
GTATCAA	19010	0.0	36.314156	1
CTTATAC	7100	0.0	30.568256	1
TCAACGC	23790	0.0	28.390263	4
ATCAACG	24025	0.0	28.13081	3
CAACGCA	24530	0.0	27.53381	5
TATCAAC	25250	0.0	26.985262	2
AACGCAG	25365	0.0	26.711466	6
ACGCAGA	28975	0.0	23.324617	7
TATACAC	11080	0.0	23.01324	3
CGCAGAG	29655	0.0	22.804153	8
GTGGTAT	5500	0.0	21.31971	1
TGGTATC	5365	0.0	20.98193	2
TTATACA	11325	0.0	20.670233	2
GTACATG	24360	0.0	19.936903	1
GCAGAGT	34255	0.0	19.692295	9
ACATCTC	11305	0.0	19.499731	8
TACATGG	24075	0.0	19.482183	2
ACACATC	11935	0.0	18.93486	6
AGAGTAC	31300	0.0	18.859621	10-11
>>END_MODULE