FastQCFastQC Report
Fri 27 May 2016
SRR2139652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10769379
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138000.052.5262571
GTATCAA256400.037.877491
TCAACGC325550.029.3053824
ATCAACG328350.029.069433
CAACGCA334150.028.5511555
TATCAAC340500.028.1836362
AACGCAG344700.027.7639926
ACGCAGA396800.024.0540227
CGCAGAG405600.023.532148
TGGTATC64800.020.949322
GCAGAGT464550.020.537089
GTGGTAT68700.020.505721
GTACATG318150.020.408691
TACATGG317900.020.0621682
GAGTACT280150.019.54859412-13
AGAGTAC422600.019.34286710-11
ACATGGG321150.019.19383
CAGAGTA455150.018.28311710-11
GTACTTT305400.017.86986714-15
AGTACTT303750.017.79788612-13
Produced by FastQC (version 0.11.5)