FastQCFastQC Report
Fri 27 May 2016
SRR2139651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8553708
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109900.051.086881
GTATCAA203050.037.348121
TCAACGC250550.029.9279274
ATCAACG252300.029.7208653
TATCAAC258400.029.1673972
CAACGCA258250.029.0191635
AACGCAG266700.028.1792936
ACGCAGA309900.024.2100247
CGCAGAG315750.023.7614778
GTGGTAT55650.020.8182741
GCAGAGT362650.020.5602669
GTACATG279250.020.4853761
TACATGG275650.019.8291952
TGGTATC53250.019.7642022
ACATGGG276000.019.404053
GAGTACT206550.019.2704512-13
AGAGTAC331650.018.34798810-11
CAGAGTA355350.018.222910-11
GTACTTT229400.017.32352314-15
CATGGGG194050.017.2105834
Produced by FastQC (version 0.11.5)