##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139651_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8553708
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58141884198058	34.0	31.0	34.0	31.0	34.0
2	32.72393200703134	34.0	31.0	34.0	31.0	34.0
3	32.79405118809293	34.0	31.0	34.0	31.0	34.0
4	36.27644981568228	37.0	37.0	37.0	35.0	37.0
5	36.206948495319224	37.0	37.0	37.0	35.0	37.0
6	36.23242165853686	37.0	37.0	37.0	35.0	37.0
7	36.17811807464084	37.0	37.0	37.0	35.0	37.0
8	36.15407493451963	37.0	37.0	37.0	35.0	37.0
9	37.872814222790865	39.0	38.0	39.0	35.0	39.0
10-11	37.8086912132142	39.0	38.0	39.0	35.0	39.0
12-13	37.778609288509735	39.0	38.0	39.0	35.0	39.0
14-15	39.15599369302763	40.0	39.0	41.0	36.0	41.0
16-17	39.117710295932476	40.0	38.0	41.0	36.0	41.0
18-19	39.00260512750728	40.0	38.5	41.0	35.5	41.0
20-21	39.06429797662557	40.0	39.0	41.0	36.0	41.0
22-23	38.98068216120518	40.0	38.0	41.0	35.0	41.0
24-25	38.87216721813762	40.0	38.0	41.0	35.0	41.0
26-27	38.82093064740995	40.0	38.0	41.0	35.0	41.0
28-29	38.70075233544334	40.0	38.0	41.0	35.0	41.0
30-31	38.63451609705993	40.0	38.0	41.0	35.0	41.0
32-33	38.505962906079105	40.0	38.0	41.0	34.0	41.0
34-35	38.27332205584591	40.0	38.0	41.0	34.0	41.0
36-37	38.54253156389811	40.0	38.0	41.0	34.0	41.0
38-39	38.63518958511444	40.0	38.0	41.0	35.0	41.0
40-41	38.62992932578874	40.0	38.0	41.0	34.5	41.0
42-43	38.56214196852706	40.0	38.0	41.0	34.0	41.0
44-45	38.48755980127098	40.0	38.0	41.0	34.0	41.0
46-47	38.41169200662682	40.0	38.0	41.0	34.0	41.0
48-49	38.34237701370226	40.0	38.0	41.0	34.0	41.0
50-51	38.26150385569153	40.0	38.0	41.0	33.0	41.0
52-53	38.16173977219873	40.0	37.0	41.0	33.0	41.0
54-55	38.03885424304589	40.0	37.0	41.0	33.0	41.0
56-57	37.88577482974553	40.0	37.0	41.0	33.0	41.0
58-59	37.7116436669868	39.5	36.0	41.0	33.0	41.0
60-61	37.5404885855947	39.0	36.0	41.0	33.0	41.0
62-63	37.320146325531496	39.0	35.5	41.0	32.0	41.0
64-65	37.031146184264436	38.5	35.0	40.0	32.0	41.0
66-67	36.70557581059086	38.0	35.0	40.0	31.0	41.0
68-69	36.38597114928815	37.0	35.0	40.0	31.0	41.0
70-71	36.04523409058221	37.0	35.0	39.5	31.0	41.0
72-73	35.7228654788956	36.0	34.5	39.0	31.0	41.0
74-75	35.39060236384891	36.0	34.0	39.0	31.0	40.0
76-77	34.48235571751035	35.0	33.5	37.0	29.5	39.0
78-79	34.71897741652646	35.0	34.0	37.0	30.0	39.0
80-81	34.52685633928621	35.0	34.0	37.0	30.0	39.0
82-83	34.27735269939118	35.0	34.0	36.0	30.0	38.5
84-85	34.024098516230914	35.0	34.0	36.0	30.0	37.0
86-87	33.820285223762006	35.0	34.0	35.5	30.0	37.0
88-89	33.618117126295374	35.0	33.5	35.0	29.5	36.5
90-91	33.44344058007667	35.0	33.0	35.0	29.0	36.0
92-93	33.31222689067259	35.0	33.0	35.0	29.0	36.0
94-95	33.25864034351583	35.0	33.0	35.0	29.0	36.0
96-97	33.255912742155694	35.0	33.0	35.0	29.0	35.0
98-99	33.3403504842314	35.0	33.0	35.0	30.0	35.0
100-101	31.766594757393506	33.5	30.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	9.0
20	51.0
21	239.0
22	1069.0
23	3644.0
24	9379.0
25	19731.0
26	34990.0
27	55694.0
28	80349.0
29	108506.0
30	139731.0
31	176834.0
32	223018.0
33	289675.0
34	398896.0
35	575700.0
36	952559.0
37	1830753.0
38	2383543.0
39	1200138.0
40	69198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.75176897028831	21.46225524494941	15.5264180843272	24.259557700435074
2	13.678810608152645	21.622169850255094	41.60216364449656	23.096855897095708
3	16.863966411147775	26.59078107035472	31.386594716387123	25.158657802110383
4	11.935950561774108	18.73092420185171	38.910401983701796	30.42272325267238
5	12.876186401097536	38.101430900410996	35.818011025631584	13.204371672859889
6	29.689508815185935	38.45236654759546	18.627027703199957	13.231096934018648
7	26.22461260938973	33.017213008865745	23.20302138079347	17.555153000951048
8	24.301718038539544	37.24091353130128	20.958781852268046	17.498586577891132
9	25.323208914863088	17.035660247343586	21.430707576805887	36.21042326098745
10-11	23.959474723259127	27.084126734812823	28.985507839396035	19.97089070253201
12-13	24.797315971038525	24.600074026375463	29.149592200248126	21.453017802337886
14-15	21.938390008002436	26.008806771849898	26.793489792947916	25.259313427199746
16-17	21.440642640933252	29.20990305544259	27.461712929376148	21.88774137424801
18-19	21.552923557686405	28.2737012791533	29.224020468323374	20.949354694836916
20-21	22.343250631279954	27.353518119080643	29.19006242564774	21.11316882399166
22-23	22.051502702930225	27.34496662186629	28.99939259338073	21.604138081822757
24-25	21.832034394295	27.508898715733793	29.086874878156856	21.572192011814355
26-27	21.77133488838801	27.63842919509072	29.025219170978634	21.565016745542636
28-29	21.715485707084817	27.631475876217127	29.06046204420461	21.59257637249344
30-31	22.01459446231966	27.494621612456427	28.95231614684845	21.53846777837546
32-33	21.687252699269468	27.759279090424627	28.929120604699516	21.62434760560639
34-35	21.916129524701176	27.713328344638512	28.87381335147919	21.496728779181122
36-37	21.806392904522856	27.645093407803728	28.885946583754237	21.662567103919184
38-39	21.710795639887017	27.669686917691827	28.97186192321131	21.647655519209845
40-41	21.92582017734428	27.613940986663405	28.796044614538935	21.664194221453375
42-43	21.86991892471798	27.700238916448956	28.60868587751591	21.82115628131715
44-45	21.942750561580652	27.80169624946318	28.412802098084654	21.842751090871516
46-47	22.08057415223403	27.72348875217817	28.119677492949215	22.076259602638586
48-49	21.99878490752858	27.86418200863674	28.07493624737038	22.062096836464296
50-51	21.92820019332446	27.985779374545206	28.018572022630124	22.067448409500212
52-53	22.132675307100648	27.90097921271651	27.930638362870457	22.035707117312384
54-55	22.11324659390618	27.845118862882188	27.91550780938029	22.126126733831335
56-57	22.147131610917913	27.996479343721724	27.795854843300553	22.060534202059806
58-59	22.022294031788586	28.04397809559077	27.892497095652857	22.041230776967787
60-61	22.164809449010527	28.09879486386037	27.773366890387628	21.96302879674147
62-63	22.127714233730778	28.175521796255687	27.744255857732362	21.95250811228117
64-65	22.085794168467558	28.083888093727875	27.760580902016663	22.0697368357879
66-67	22.166896845161506	28.078545243151538	27.612892170767456	22.1416657409195
68-69	22.098666976238352	28.00949792467224	27.738947633054757	22.152887466034656
70-71	22.09559665303781	27.813672942393087	27.81422432741022	22.27650607715889
72-73	22.19206103147371	27.864825039625174	27.676881542507793	22.266232386393327
74-75	22.0229884927975	28.00126547330159	27.67289866968281	22.3028473642181
76-77	22.173994322282443	27.9061070844469	27.656135453101875	22.263763140168777
78-79	22.136324685488457	27.89925607657709	27.589408590347308	22.37501064758715
80-81	22.148089858386495	27.876736202475694	27.65227391702323	22.322900022114585
82-83	22.179952437267193	27.84517205052941	27.72122113349852	22.253654378704883
84-85	22.222539345208112	27.80165331985677	27.712257014266502	22.26355032066862
86-87	22.119404417461194	27.804478131123943	27.78595018829173	22.290167263123127
88-89	22.186915364214062	27.782763685010565	27.724140365931394	22.306180584843975
90-91	22.149988456142776	27.815504686369536	27.76981252495258	22.26469433253511
92-93	22.228578056609948	27.70271154165877	27.789240743123628	22.279469658607653
94-95	22.050739705073973	27.702024459391634	27.86030545494946	22.38693038058493
96-97	22.0346838354085	27.726797501973827	27.946680216880488	22.291838445737188
98-99	22.177067187699574	28.310141155270795	27.977456236342736	21.535335420686895
100-101	20.53424359670187	31.89069427053156	25.901928474980867	21.673133657785705
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2325.0
1	1887.5
2	2064.5
3	2836.0
4	3733.5
5	5516.5
6	7126.0
7	8904.0
8	10624.0
9	11331.0
10	10844.0
11	10089.5
12	11146.0
13	15115.5
14	24853.5
15	38024.0
16	48224.0
17	53290.5
18	53801.0
19	51215.0
20	46755.0
21	42087.5
22	39811.5
23	42166.5
24	49232.0
25	60844.0
26	76150.5
27	93076.0
28	110843.5
29	130023.5
30	148603.5
31	167659.0
32	189271.0
33	211378.5
34	232944.5
35	253155.0
36	275368.5
37	292687.0
38	304164.5
39	315578.5
40	326370.5
41	336038.0
42	340202.0
43	344890.0
44	350757.5
45	354752.0
46	357097.0
47	355428.0
48	351884.0
49	347754.0
50	341248.5
51	329685.5
52	313996.5
53	295653.5
54	276468.0
55	256521.0
56	234255.0
57	210833.5
58	187127.0
59	162075.0
60	135732.5
61	110349.0
62	87103.5
63	66879.0
64	49869.0
65	37166.0
66	27964.0
67	21074.0
68	15989.5
69	11691.5
70	8375.0
71	5951.5
72	4022.0
73	2654.0
74	1748.5
75	1137.0
76	769.0
77	482.5
78	306.0
79	219.5
80	151.0
81	102.0
82	75.0
83	59.0
84	43.5
85	27.5
86	18.5
87	13.5
88	8.0
89	4.5
90	3.5
91	2.5
92	1.0
93	1.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.507250890491001E-5
2	0.0042320827411924744
3	0.004606189502844848
4	3.507250890491001E-5
5	3.507250890491001E-5
6	3.507250890491001E-5
7	3.507250890491001E-5
8	0.0
9	0.003062999111028808
10-11	9.76184831186662E-4
12-13	0.0
14-15	7.365226870031103E-4
16-17	5.260876335736502E-5
18-19	3.507250890491001E-5
20-21	5.8455903672153106E-6
22-23	6.021415309699804E-4
24-25	0.004209879816117128
26-27	0.02181054677093071
28-29	0.3395435509373263
30-31	0.3315550059843593
32-33	0.3421172166982885
34-35	0.18171957483636966
36-37	0.03978100109527834
38-39	0.036247954324841854
40-41	0.012505853326036155
42-43	0.010172422869126141
44-45	0.009447253544088804
46-47	0.005863018500711859
48-49	0.008955973662362027
50-51	0.009016069453287463
52-53	0.002909655328288963
54-55	0.0036335438073659253
56-57	0.0035056371094160065
58-59	0.0041475065903231675
60-61	0.0028653954952319294
62-63	0.0013604210331393941
64-65	4.4010767967896146E-4
66-67	0.006834782545394347
68-69	0.016371171117382864
70-71	0.013727602197243316
72-73	0.01534608486405828
74-75	0.007815299272099924
76-77	0.004883299117019792
78-79	0.0017352655791567676
80-81	0.0013192770798931604
82-83	7.232139806413329E-4
84-85	1.3448653042683035E-4
86-87	0.0
88-89	4.067849269759655E-4
90-91	0.005011700332744166
92-93	0.018437769239189945
94-95	0.0016471351958879754
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	161.0
20-21	510.0
22-23	1119.0
24-25	2499.0
26-27	4083.0
28-29	7182.0
30-31	16748.0
32-33	22238.0
34-35	32262.0
36-37	45806.0
38-39	55060.0
40-41	57668.0
42-43	69108.0
44-45	82363.0
46-47	82254.0
48-49	72406.0
50-51	64716.0
52-53	63331.0
54-55	68579.0
56-57	71905.0
58-59	72491.0
60-61	72427.0
62-63	71641.0
64-65	77894.0
66-67	83051.0
68-69	80822.0
70-71	78568.0
72-73	76233.0
74-75	77313.0
76-77	78345.0
78-79	83721.0
80-81	85114.0
82-83	83245.0
84-85	84663.0
86-87	89963.0
88-89	97413.0
90-91	106223.0
92-93	111139.0
94-95	128410.0
96-97	195300.0
98-99	762930.0
100-101	5136804.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.78515463793663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.6401895457472	78.66222866139559
2	6.725032221519414	11.672659225793376
3	1.5376892126542199	4.003457883158505
4	0.5212103353777001	1.8093327821857803
5	0.233851144498615	1.0147403868785383
6	0.11357598176950665	0.5914025484613263
7	0.07577612716146834	0.46033700395003857
8	0.03900718215600185	0.27081954683190257
9	0.02678089420103583	0.20917656401212031
>10	0.08460664728747297	1.120153076311398
>50	0.0018630128136179315	0.10804991082139348
>100	3.9721927511298164E-4	0.06523914013639424
>500	2.0475538655203862E-5	0.012403270063638673
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3381672603273342E-5
2	0.0	0.0	0.0	0.0	2.3381672603273342E-5
3	0.0	0.0	0.0	0.0	2.3381672603273342E-5
4	0.0	4.6763345206546683E-5	0.0	2.3381672603273342E-5	3.507250890491001E-5
5	0.0	5.8454181508183354E-5	0.0	2.3381672603273342E-5	3.507250890491001E-5
6	5.8454181508183354E-5	5.8454181508183354E-5	0.0	2.3381672603273342E-5	5.8454181508183354E-5
7	5.8454181508183354E-5	5.8454181508183354E-5	0.0	3.507250890491001E-5	5.8454181508183354E-5
8	5.8454181508183354E-5	9.352669041309337E-5	0.0	3.507250890491001E-5	5.8454181508183354E-5
9	5.8454181508183354E-5	1.4029003561964005E-4	0.0	4.6763345206546683E-5	5.8454181508183354E-5
10-11	5.8454181508183354E-5	1.4029003561964005E-4	0.0	5.8454181508183354E-5	5.8454181508183354E-5
12-13	5.8454181508183354E-5	1.4029003561964005E-4	0.0	6.429959965900168E-5	6.429959965900168E-5
14-15	6.429959965900168E-5	2.0458963527864173E-4	0.0	7.599043596063837E-5	7.014501780982003E-5
16-17	9.937210856391171E-5	2.2212588973109673E-4	0.0	8.768127226227502E-5	8.18358541114567E-5
18-19	1.0521752671473004E-4	2.455075623343701E-4	0.0	1.0521752671473004E-4	8.18358541114567E-5
20-21	1.0521752671473004E-4	2.805800712392801E-4	0.0	1.4613545377045837E-4	9.937210856391171E-5
22-23	1.1690836301636671E-4	3.7410676165237347E-4	0.0	1.7536254452455005E-4	1.2275378116718505E-4
24-25	1.344446174688217E-4	4.3840636131137515E-4	0.0	2.1043505342946008E-4	1.2859919931800337E-4
26-27	1.519808719212767E-4	4.5594261576383015E-4	0.0	3.565705071999184E-4	1.2859919931800337E-4
28-29	1.519808719212767E-4	4.6763345206546683E-4	0.0	7.014501780982002E-4	1.4029003561964005E-4
30-31	1.519808719212767E-4	5.611601424785602E-4	0.0	0.001741934608943864	1.519808719212767E-4
32-33	1.5782629007209505E-4	5.611601424785602E-4	0.0	0.0037819855435794626	1.519808719212767E-4
34-35	1.812079626753684E-4	6.488414147408353E-4	0.0	0.006505950401860807	1.519808719212767E-4
36-37	1.987442171278234E-4	7.540589414555652E-4	0.0	0.010872477760522104	1.519808719212767E-4
38-39	1.987442171278234E-4	7.949768685112936E-4	0.0	0.017577172379510734	1.519808719212767E-4
40-41	1.987442171278234E-4	8.885035589243869E-4	0.0	0.0249950080128992	1.519808719212767E-4
42-43	1.987442171278234E-4	9.586485767342069E-4	0.0	0.03226086277436639	1.519808719212767E-4
44-45	2.1628047158027842E-4	0.0011281657031079386	0.0	0.04069580116599725	1.519808719212767E-4
46-47	2.3381672603273342E-4	0.0012275378116718503	0.0	0.05031735944224423	1.5782629007209505E-4
48-49	2.3381672603273342E-4	0.0012275378116718503	0.0	0.05977524601026829	1.7536254452455005E-4
50-51	2.3381672603273342E-4	0.0014087457743472188	0.0	0.06946110388617427	1.812079626753684E-4
52-53	2.3381672603273342E-4	0.0014262820287996739	0.0	0.07838705740247387	2.1043505342946008E-4
54-55	2.3381672603273342E-4	0.0014730453740062206	0.0	0.08714349379239975	2.3381672603273342E-4
56-57	2.3381672603273342E-4	0.0015314995555144038	0.0	0.09409954139187356	2.455075623343701E-4
58-59	2.455075623343701E-4	0.0015607266462684954	0.0	0.09995080496084272	2.5719839863600673E-4
60-61	2.513529804851884E-4	0.0015782629007209505	0.0	0.10548641594966768	2.5719839863600673E-4
62-63	2.5719839863600673E-4	0.0016133354096258606	0.0	0.11102787235664346	2.5719839863600673E-4
64-65	2.5719839863600673E-4	0.0018997608990159588	0.0	0.11628290327422913	2.5719839863600673E-4
66-67	2.5719839863600673E-4	0.0019289879897700507	0.0	0.12206402182538847	2.5719839863600673E-4
68-69	2.5719839863600673E-4	0.001952369662373324	0.0	0.12792113081250844	2.5719839863600673E-4
70-71	2.5719839863600673E-4	0.001981596753127416	0.0	0.13333398802016622	2.747346530884618E-4
72-73	3.039617438425534E-4	0.0019991330075798705	0.0	0.13774727872403408	3.039617438425534E-4
74-75	3.039617438425534E-4	0.0020342055164847806	0.0	0.141611100121725	3.039617438425534E-4
76-77	3.039617438425534E-4	0.0021043505342946004	0.0	0.14529371355674053	3.039617438425534E-4
78-79	3.039617438425534E-4	0.0021160413705962372	0.0	0.148368403504071	3.039617438425534E-4
80-81	3.039617438425534E-4	0.0021160413705962372	0.0	0.1499934297499985	3.156525801441901E-4
82-83	3.039617438425534E-4	0.002145268461350329	0.0	0.15051367196542131	3.390342527474634E-4
84-85	3.039617438425534E-4	0.002162804715802784	0.0	0.15096376916303433	3.448796708982818E-4
86-87	3.039617438425534E-4	0.002232949733612604	0.0	0.15109236836235232	3.507250890491001E-4
88-89	3.039617438425534E-4	0.002262176824366696	0.0	0.15112744087125723	3.507250890491001E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10990	0.0	51.08688	1
GTATCAA	20305	0.0	37.34812	1
TCAACGC	25055	0.0	29.927927	4
ATCAACG	25230	0.0	29.720865	3
TATCAAC	25840	0.0	29.167397	2
CAACGCA	25825	0.0	29.019163	5
AACGCAG	26670	0.0	28.179293	6
ACGCAGA	30990	0.0	24.210024	7
CGCAGAG	31575	0.0	23.761477	8
GTGGTAT	5565	0.0	20.818274	1
GCAGAGT	36265	0.0	20.560266	9
GTACATG	27925	0.0	20.485376	1
TACATGG	27565	0.0	19.829195	2
TGGTATC	5325	0.0	19.764202	2
ACATGGG	27600	0.0	19.40405	3
GAGTACT	20655	0.0	19.27045	12-13
AGAGTAC	33165	0.0	18.347988	10-11
CAGAGTA	35535	0.0	18.2229	10-11
GTACTTT	22940	0.0	17.323523	14-15
CATGGGG	19405	0.0	17.210583	4
>>END_MODULE