FastQCFastQC Report
Fri 27 May 2016
SRR2139650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8392660
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104700.052.1835141
GTATCAA196550.037.868221
TCAACGC245250.029.7724824
ATCAACG247400.029.5310273
CAACGCA251500.029.0326085
TATCAAC258050.028.4621962
AACGCAG259300.028.25826
ACGCAGA298500.024.5329177
CGCAGAG307300.023.7886498
TGGTATC50700.021.41782
GTGGTAT55000.021.375641
GCAGAGT350550.020.7198859
GTACATG248850.020.5810871
TACATGG249450.020.1031282
GAGTACT211550.019.328612-13
ACATGGG251650.019.2472723
AGAGTAC318400.018.97808810-11
CAGAGTA345300.018.1248410-11
GTACTTT230450.017.73443414-15
AGTACTT230750.016.75696412-13
Produced by FastQC (version 0.11.5)