##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139650_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8392660
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59250142386323	34.0	31.0	34.0	31.0	34.0
2	32.735115088660805	34.0	31.0	34.0	31.0	34.0
3	32.805757650137146	34.0	31.0	34.0	31.0	34.0
4	36.28224853622093	37.0	37.0	37.0	35.0	37.0
5	36.21375428052608	37.0	37.0	37.0	35.0	37.0
6	36.24075108487655	37.0	37.0	37.0	35.0	37.0
7	36.18989760099897	37.0	37.0	37.0	35.0	37.0
8	36.16679813074758	37.0	37.0	37.0	35.0	37.0
9	37.89147540827342	39.0	38.0	39.0	35.0	39.0
10-11	37.829466462361154	39.0	38.0	39.0	35.0	39.0
12-13	37.79706803325763	39.0	38.0	39.0	35.0	39.0
14-15	39.17978239318643	40.0	39.0	41.0	36.0	41.0
16-17	39.14134863082741	40.0	39.0	41.0	36.0	41.0
18-19	39.02561750386647	40.0	38.5	41.0	36.0	41.0
20-21	39.08660131480409	40.0	39.0	41.0	36.0	41.0
22-23	39.00233792694098	40.0	38.5	41.0	35.0	41.0
24-25	38.8926357458498	40.0	38.0	41.0	35.0	41.0
26-27	38.84276808732726	40.0	38.0	41.0	35.0	41.0
28-29	38.73062838664561	40.0	38.0	41.0	35.0	41.0
30-31	38.663893272589576	40.0	38.0	41.0	35.0	41.0
32-33	38.535400329093065	40.0	38.0	41.0	34.0	41.0
34-35	38.30393479507174	40.0	38.0	41.0	34.0	41.0
36-37	38.56698477235962	40.0	38.0	41.0	34.5	41.0
38-39	38.659299698297616	40.0	38.0	41.0	35.0	41.0
40-41	38.650893848691695	40.0	38.0	41.0	35.0	41.0
42-43	38.57799165399129	40.0	38.0	41.0	34.0	41.0
44-45	38.50272331992254	40.0	38.0	41.0	34.0	41.0
46-47	38.42647322360068	40.0	38.0	41.0	34.0	41.0
48-49	38.35615756218766	40.0	38.0	41.0	34.0	41.0
50-51	38.275219958353006	40.0	38.0	41.0	33.5	41.0
52-53	38.173020071948955	40.0	37.5	41.0	33.0	41.0
54-55	38.04945301689686	40.0	37.0	41.0	33.0	41.0
56-57	37.898020133787014	40.0	37.0	41.0	33.0	41.0
58-59	37.72294033426147	39.5	36.0	41.0	33.0	41.0
60-61	37.55037850281331	39.0	36.0	41.0	33.0	41.0
62-63	37.32684173457741	39.0	35.5	41.0	32.0	41.0
64-65	37.03578966001167	38.5	35.0	40.0	32.0	41.0
66-67	36.71042224016804	38.0	35.0	40.0	31.5	41.0
68-69	36.39008042700962	37.0	35.0	40.0	31.0	41.0
70-71	36.04867792805543	37.0	35.0	39.5	31.0	41.0
72-73	35.72675911139467	36.0	34.5	39.0	31.0	41.0
74-75	35.3967524510573	36.0	34.0	39.0	31.0	40.0
76-77	34.4841693420874	35.0	33.5	37.0	29.5	39.0
78-79	34.723997791667045	35.0	34.0	37.0	30.0	39.0
80-81	34.52941534388121	35.0	34.0	37.0	30.0	39.0
82-83	34.28209038981723	35.0	34.0	36.0	30.0	38.5
84-85	34.02610776284763	35.0	34.0	36.0	30.0	37.0
86-87	33.8195547094038	35.0	34.0	35.5	30.0	37.0
88-89	33.61626192514781	35.0	33.0	35.0	29.5	36.5
90-91	33.43761917459899	35.0	33.0	35.0	29.0	36.0
92-93	33.30677880810978	35.0	33.0	35.0	29.0	36.0
94-95	33.25126493847178	35.0	33.0	35.0	29.0	36.0
96-97	33.246855052521106	35.0	33.0	35.0	29.0	35.0
98-99	33.333336744701356	35.0	33.0	35.0	29.5	35.0
100-101	31.738529923635156	33.5	30.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	6.0
20	39.0
21	205.0
22	1075.0
23	3567.0
24	9325.0
25	19326.0
26	34284.0
27	54256.0
28	78449.0
29	106112.0
30	137007.0
31	172652.0
32	217284.0
33	281372.0
34	389773.0
35	565140.0
36	939197.0
37	1818458.0
38	2359886.0
39	1142656.0
40	62589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.94118193755019	21.21527620563683	15.468421215681321	24.375120641131655
2	13.67516967894456	21.545186551513765	41.50399412796462	23.27564964157706
3	16.910501544108158	26.431852442433907	31.234930244346465	25.422715769111477
4	11.959295384300091	18.64256385937236	38.86616400521408	30.531976751113472
5	12.958430342704219	37.97222811361356	35.77384285792585	13.295498685756362
6	29.84299375883212	38.35795802522681	18.570357907981496	13.228690307959573
7	26.329459313257058	32.89074024206867	23.115496159739582	17.664304284934694
8	24.329318714209798	37.161889079266885	20.88038833933461	17.62840386718871
9	25.326699880797083	16.949120229083128	21.345937258561662	36.37824263155813
10-11	23.962336193582736	27.06324102406576	28.947259458712658	20.02716332363884
12-13	24.79332535811054	24.584923016064035	29.102811266034845	21.518940359790577
14-15	21.885449310755824	26.02304721100103	26.784253602489528	25.307249875753623
16-17	21.42170998589957	29.17058775448148	27.490737733260538	21.91696452635841
18-19	21.501127622567704	28.2253028912475	29.3308704662379	20.9426990199469
20-21	22.241633911922694	27.349628349286014	29.306566172844246	21.102171565947046
22-23	21.945318251551104	27.29223125833705	29.14928552515456	21.61316496495728
24-25	21.75889793518988	27.43533791247692	29.211677006847736	21.594087145485467
26-27	21.68278969958361	27.593261328481468	29.149217231198897	21.574731740736024
28-29	21.6465348130346	27.59572362910901	29.165971706425577	21.59176985143082
30-31	21.91042490128344	27.48625947307278	29.080850086509713	21.52246553913406
32-33	21.639897020444344	27.660211646049166	29.036421793589607	21.663469539916882
34-35	21.859239621037606	27.67587896365475	28.978143043893105	21.48673837141454
36-37	21.78522531848966	27.591711756390065	28.953373810163257	21.669689114957016
38-39	21.637583131684973	27.683151653476166	29.042947905708694	21.63631730913016
40-41	21.8800354644128	27.60752107275889	28.883129919025606	21.629313543802713
42-43	21.81444683460867	27.704168447636484	28.66070640546226	21.82067831229259
44-45	21.905471646070325	27.778353620449316	28.48661743005676	21.8295573034236
46-47	21.979400096110904	27.76967626010688	28.195088153281905	22.055835490500307
48-49	21.90080988245675	27.90290234141115	28.142016197649134	22.054271578482968
50-51	21.92073094204172	27.995777942929152	28.043094976266815	22.040396138762315
52-53	22.079010541670378	27.914875320239986	27.961451607781434	22.044662530308198
54-55	22.05824759462749	27.87913005148548	27.960443960395708	22.102178393491325
56-57	22.058953896817187	27.96878901397643	27.921947039170288	22.0503100500361
58-59	21.999496337954476	28.04599264947293	27.92615152014691	22.028359492425686
60-61	22.106794786708846	28.062107165330836	27.835770874040804	21.995327173919517
62-63	22.075836801023907	28.138932417176893	27.83279717784517	21.952433603954027
64-65	22.076822054734137	27.998640515467404	27.821236807686812	22.10330062211165
66-67	22.098460185453998	28.04639621048242	27.725325805329952	22.12981779873363
68-69	22.053842919026902	28.023395591087752	27.81006821927809	22.11269327060726
70-71	22.041380233142483	27.831327416689494	27.86842239865106	22.258869951516964
72-73	22.092584546416987	27.83969672102532	27.804596545671657	22.263122186886037
74-75	21.994794760609324	28.010029887331196	27.739630139586303	22.255545212473177
76-77	22.095782635674617	27.90357367535246	27.72973474006552	22.270908948907405
78-79	22.051484089233305	27.90207758034901	27.701642401723365	22.34479592869432
80-81	22.051100211071493	27.931360457031563	27.718037144781004	22.299502187115937
82-83	22.110454273278723	27.88076657728255	27.765210190736084	22.243568958702646
84-85	22.156428129724173	27.881845970595048	27.74172333335952	22.220002566321263
86-87	22.060496937997527	27.871951260612267	27.81849752282292	22.249054278567286
88-89	22.088928378434073	27.808301845435267	27.83020580279884	22.272563973331817
90-91	22.094891679735127	27.858987083561527	27.81917422269906	22.226947014004285
92-93	22.139418038501503	27.738875615734692	27.89406387113565	22.227642474628155
94-95	21.997098917909913	27.74278156872051	27.927184257215533	22.332935256154045
96-97	21.96468480842946	27.79938151622275	28.01905511462346	22.21687856072433
98-99	22.115590257500443	28.33494672954684	28.03375172708783	21.515711285864885
100-101	20.463531575614518	31.964034774659012	25.994503471782192	21.57793017794428
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2568.0
1	2065.0
2	2139.5
3	2909.5
4	3856.0
5	5728.5
6	7470.5
7	9612.0
8	11633.0
9	12159.5
10	11242.0
11	10295.5
12	11431.5
13	15407.5
14	24892.5
15	37666.5
16	47573.5
17	52485.0
18	52778.5
19	50044.5
20	45652.0
21	41075.5
22	38902.0
23	41380.5
24	48359.5
25	59710.5
26	74505.0
27	90279.0
28	107607.0
29	126616.0
30	144915.0
31	163558.0
32	183918.0
33	205393.5
34	225901.0
35	243990.5
36	264646.5
37	281650.0
38	293584.0
39	305854.0
40	315875.0
41	324706.5
42	329505.5
43	333871.0
44	338548.5
45	342020.5
46	344671.0
47	343731.0
48	340287.0
49	335734.5
50	330157.5
51	320480.5
52	306164.0
53	288598.0
54	270247.5
55	251498.5
56	230215.0
57	206451.0
58	182881.0
59	158351.0
60	132693.0
61	108286.5
62	85738.0
63	65722.0
64	48585.0
65	35904.5
66	26856.5
67	20356.0
68	15578.5
69	11347.0
70	8003.5
71	5783.0
72	4037.5
73	2673.5
74	1752.5
75	1116.5
76	703.0
77	461.5
78	301.0
79	198.5
80	133.0
81	92.0
82	69.0
83	48.0
84	34.0
85	23.5
86	15.5
87	11.5
88	8.0
89	6.0
90	4.5
91	4.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004051158988926037
3	0.005206930818119643
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0029549630272166392
10-11	0.0011140687219546605
12-13	0.0
14-15	7.268255833073185E-4
16-17	7.149104098104772E-5
18-19	2.9787933742103217E-5
20-21	0.0
22-23	5.958191607815623E-4
24-25	0.00432047225085818
26-27	0.021382829427017667
28-29	0.3356106401241523
30-31	0.32801862907940255
32-33	0.33875026666301344
34-35	0.18018054765097063
36-37	0.03939016722423736
38-39	0.0361385852079909
40-41	0.013736216878965838
42-43	0.010615691721784245
44-45	0.009300574533097553
46-47	0.006156413615589288
48-49	0.009698740972340691
50-51	0.009829741506391683
52-53	0.003380267840131769
54-55	0.004134871909925073
56-57	0.0042469467509621646
58-59	0.004775312056475674
60-61	0.0033678679853976125
62-63	0.001701946005062188
64-65	7.407479237509104E-4
66-67	0.0064873611720009565
68-69	0.015441882024708246
70-71	0.012344962811059755
72-73	0.013759169947056985
74-75	0.007078952906770262
76-77	0.004248166744406688
78-79	0.0013734684922713481
80-81	0.0010747203332773553
82-83	5.916830661489834E-4
84-85	1.2719360332894092E-4
86-87	0.0
88-89	4.297884865036822E-4
90-91	0.0046415030744506435
92-93	0.017466115380034418
94-95	0.0015304230379251718
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	137.0
20-21	507.0
22-23	1064.0
24-25	2588.0
26-27	4038.0
28-29	6608.0
30-31	15385.0
32-33	20004.0
34-35	29742.0
36-37	41781.0
38-39	49837.0
40-41	53005.0
42-43	63767.0
44-45	76669.0
46-47	76128.0
48-49	67224.0
50-51	59764.0
52-53	58087.0
54-55	62016.0
56-57	65248.0
58-59	65786.0
60-61	65792.0
62-63	65333.0
64-65	70601.0
66-67	74305.0
68-69	73537.0
70-71	72862.0
72-73	69903.0
74-75	71653.0
76-77	73276.0
78-79	77309.0
80-81	78993.0
82-83	77428.0
84-85	79525.0
86-87	85275.0
88-89	92477.0
90-91	101368.0
92-93	106612.0
94-95	123585.0
96-97	192622.0
98-99	766532.0
100-101	5154287.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.08613233356779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.78558627187266	79.06165580052836
2	6.632635598648659	11.552211629284995
3	1.5344291879944665	4.008825099665252
4	0.5037410621336996	1.754754431953094
5	0.22128160481793505	0.9635279560079473
6	0.11600288427014337	0.6061345518375152
7	0.0628032825604462	0.3828506483229028
8	0.03949073143788051	0.27512760511589185
9	0.022609447872824123	0.1772072432497498
>10	0.07968676424500142	1.0703810601610169
>50	0.001432832090010584	0.08094056374858158
>100	2.7994375826761244E-4	0.0545405716063833
>500	2.0388297897338408E-5	0.011842838518321241
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3830346993682574E-5	0.0	0.0	0.0
3	0.0	2.3830346993682574E-5	0.0	0.0	0.0
4	0.0	4.766069398736515E-5	0.0	1.1915173496841287E-5	0.0
5	0.0	4.766069398736515E-5	0.0	1.1915173496841287E-5	0.0
6	3.574552049052386E-5	4.766069398736515E-5	0.0	1.1915173496841287E-5	0.0
7	3.574552049052386E-5	4.766069398736515E-5	0.0	1.1915173496841287E-5	0.0
8	4.766069398736515E-5	4.766069398736515E-5	0.0	1.1915173496841287E-5	0.0
9	4.766069398736515E-5	7.149104098104772E-5	0.0	1.1915173496841287E-5	0.0
10-11	5.9575867484206435E-5	8.340621447788902E-5	0.0	1.1915173496841287E-5	0.0
12-13	5.9575867484206435E-5	8.936380122630966E-5	0.0	1.1915173496841287E-5	0.0
14-15	6.553345423262708E-5	1.7277001570419867E-4	0.0	2.3830346993682574E-5	0.0
16-17	7.744862772946838E-5	1.787276024526193E-4	0.0	4.766069398736515E-5	0.0
18-19	8.340621447788902E-5	2.0255794944630187E-4	0.0	5.9575867484206435E-5	0.0
20-21	8.936380122630966E-5	2.2638829643998446E-4	0.0	7.744862772946838E-5	0.0
22-23	9.53213879747303E-5	2.3830346993682574E-4	0.0	9.53213879747303E-5	0.0
24-25	1.0127897472315094E-4	2.8596416392419094E-4	0.0	9.53213879747303E-5	0.0
26-27	1.0723656147157159E-4	3.097945109178735E-4	0.0	1.2510932171683352E-4	0.0
28-29	1.0723656147157159E-4	3.1575209766629414E-4	0.0	4.0511589889260374E-4	0.0
30-31	1.0723656147157159E-4	3.4554003140839734E-4	0.0	0.001239178043671494	0.0
32-33	1.3106690846525416E-4	3.574552049052386E-4	0.0	0.0031158178694239967	0.0
34-35	1.370244952136748E-4	3.6341279165365926E-4	0.0	0.005719283278483818	1.1915173496841287E-5
36-37	1.4298208196209544E-4	3.812855518989212E-4	0.0	0.009401071889007776	1.1915173496841287E-5
38-39	1.4298208196209544E-4	4.0511589889260374E-4	0.0	0.01544802243865473	1.1915173496841287E-5
40-41	1.5489725545893675E-4	4.2894624588628636E-4	0.0	0.02222775615835742	1.1915173496841287E-5
42-43	1.5489725545893675E-4	4.4086141938312764E-4	0.0	0.0283104522284949	1.1915173496841287E-5
44-45	1.6681242895577803E-4	5.18310047112596E-4	0.0	0.035787223597762804	2.3830346993682574E-5
46-47	1.787276024526193E-4	5.600131543515405E-4	0.0	0.044461469903463266	2.3830346993682574E-5
48-49	1.787276024526193E-4	5.659707410999612E-4	0.0	0.052700812376529016	3.574552049052386E-5
50-51	1.787276024526193E-4	6.612921290746914E-4	0.0	0.06043971756272743	3.574552049052386E-5
52-53	1.787276024526193E-4	6.672497158231121E-4	0.0	0.06870289038278686	3.574552049052386E-5
54-55	1.787276024526193E-4	6.732073025715328E-4	0.0	0.07672180214616105	3.574552049052386E-5
56-57	1.787276024526193E-4	7.02995236313636E-4	0.0	0.08333472343690797	3.574552049052386E-5
58-59	1.787276024526193E-4	7.268255833073185E-4	0.0	0.08845824804054972	3.574552049052386E-5
60-61	1.8468518920103995E-4	7.387407568041598E-4	0.0	0.09351028160321043	3.574552049052386E-5
62-63	1.906427759494606E-4	7.625711037978424E-4	0.0	0.09860401827311008	4.766069398736515E-5
64-65	1.906427759494606E-4	8.69807665269414E-4	0.0	0.10353094251405395	4.766069398736515E-5
66-67	1.906427759494606E-4	8.69807665269414E-4	0.0	0.10870212781168306	4.766069398736515E-5
68-69	2.025579494463019E-4	8.69807665269414E-4	0.0	0.1139388465635448	4.766069398736515E-5
70-71	2.025579494463019E-4	8.69807665269414E-4	0.0	0.1192172684226455	4.766069398736515E-5
72-73	2.025579494463019E-4	8.69807665269414E-4	0.0	0.12335183362604943	4.766069398736515E-5
74-75	2.025579494463019E-4	8.817228387662553E-4	0.0	0.12658680323044186	4.766069398736515E-5
76-77	2.0851553619472254E-4	9.174683592567791E-4	0.0	0.12959538453839425	4.766069398736515E-5
78-79	2.1447312294314318E-4	9.174683592567791E-4	0.0	0.1322345954679446	4.766069398736515E-5
80-81	2.1447312294314318E-4	9.174683592567791E-4	0.0	0.13351547661885504	4.766069398736515E-5
82-83	2.2043070969156382E-4	9.174683592567791E-4	0.0	0.13401591390572237	5.9575867484206435E-5
84-85	2.323458831884051E-4	9.174683592567791E-4	0.0	0.13432570841664027	5.9575867484206435E-5
86-87	2.3830346993682574E-4	9.293835327536204E-4	0.0	0.13444486015160867	5.9575867484206435E-5
88-89	2.3830346993682574E-4	9.591714664957235E-4	0.0	0.13447464808535078	5.9575867484206435E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10470	0.0	52.183514	1
GTATCAA	19655	0.0	37.86822	1
TCAACGC	24525	0.0	29.772482	4
ATCAACG	24740	0.0	29.531027	3
CAACGCA	25150	0.0	29.032608	5
TATCAAC	25805	0.0	28.462196	2
AACGCAG	25930	0.0	28.2582	6
ACGCAGA	29850	0.0	24.532917	7
CGCAGAG	30730	0.0	23.788649	8
TGGTATC	5070	0.0	21.4178	2
GTGGTAT	5500	0.0	21.37564	1
GCAGAGT	35055	0.0	20.719885	9
GTACATG	24885	0.0	20.581087	1
TACATGG	24945	0.0	20.103128	2
GAGTACT	21155	0.0	19.3286	12-13
ACATGGG	25165	0.0	19.247272	3
AGAGTAC	31840	0.0	18.978088	10-11
CAGAGTA	34530	0.0	18.12484	10-11
GTACTTT	23045	0.0	17.734434	14-15
AGTACTT	23075	0.0	16.756964	12-13
>>END_MODULE