FastQCFastQC Report
Fri 27 May 2016
SRR2139649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10174942
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127700.050.8075871
GTATCAA231650.036.7758331
CTTATAC94900.035.54961
TCAACGC285700.029.2958814
ATCAACG288050.029.0727673
CAACGCA293600.028.5076055
TATACAC137800.028.2431983
TATCAAC298900.028.1461962
AACGCAG303250.027.712526
TTATACA145250.024.6599312
ACGCAGA347050.024.166057
CGCAGAG351900.023.8329858
ACATCTC161050.021.6059688
TACACAT179500.021.444425
ACACATC161800.021.2957486
GCAGAGT407600.020.482619
GTACATG281200.020.096331
GTGGTAT64150.019.5391561
GAGTACT248850.019.5311712-13
TACATGG285200.019.218552
Produced by FastQC (version 0.11.5)