##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139649_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10174942
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58552176513635	34.0	31.0	34.0	31.0	34.0
2	32.728985383897026	34.0	31.0	34.0	31.0	34.0
3	32.80005674725222	34.0	31.0	34.0	31.0	34.0
4	36.27759588211903	37.0	37.0	37.0	35.0	37.0
5	36.209635003324834	37.0	37.0	37.0	35.0	37.0
6	36.2358186415215	37.0	37.0	37.0	35.0	37.0
7	36.18306669463079	37.0	37.0	37.0	35.0	37.0
8	36.16129605456228	37.0	37.0	37.0	35.0	37.0
9	37.88261977316431	39.0	38.0	39.0	35.0	39.0
10-11	37.819234890970385	39.0	38.0	39.0	35.0	39.0
12-13	37.78725765709524	39.0	38.0	39.0	35.0	39.0
14-15	39.166437803773235	40.0	39.0	41.0	36.0	41.0
16-17	39.1267361032623	40.0	38.5	41.0	36.0	41.0
18-19	39.01291299744018	40.0	38.5	41.0	35.5	41.0
20-21	39.078292866017804	40.0	39.0	41.0	36.0	41.0
22-23	38.99259662148704	40.0	38.5	41.0	35.0	41.0
24-25	38.88134450398384	40.0	38.0	41.0	35.0	41.0
26-27	38.82840017500578	40.0	38.0	41.0	35.0	41.0
28-29	38.71710141774951	40.0	38.0	41.0	35.0	41.0
30-31	38.64547038781184	40.0	38.0	41.0	35.0	41.0
32-33	38.51454222632788	40.0	38.0	41.0	34.0	41.0
34-35	38.279211024638144	40.0	38.0	41.0	34.0	41.0
36-37	38.542306481284484	40.0	38.0	41.0	34.0	41.0
38-39	38.62432482692772	40.0	38.0	41.0	35.0	41.0
40-41	38.62909382184347	40.0	38.0	41.0	34.5	41.0
42-43	38.56370891966115	40.0	38.0	41.0	34.0	41.0
44-45	38.49287220176358	40.0	38.0	41.0	34.0	41.0
46-47	38.41955407312139	40.0	38.0	41.0	34.0	41.0
48-49	38.35263426429065	40.0	38.0	41.0	34.0	41.0
50-51	38.26431029155325	40.0	38.0	41.0	33.0	41.0
52-53	38.167883111524176	40.0	37.5	41.0	33.0	41.0
54-55	38.051148015189824	40.0	37.0	41.0	33.0	41.0
56-57	37.89569171449015	40.0	37.0	41.0	33.0	41.0
58-59	37.722009720460115	39.5	36.0	41.0	33.0	41.0
60-61	37.551963789591966	39.0	36.0	41.0	33.0	41.0
62-63	37.33868043469131	39.0	35.5	41.0	32.0	41.0
64-65	37.06356619476058	38.5	35.0	40.0	32.0	41.0
66-67	36.7473933537085	38.0	35.0	40.0	31.5	41.0
68-69	36.43497927415878	37.5	35.0	40.0	31.0	41.0
70-71	36.09994823692638	37.0	35.0	39.5	31.0	41.0
72-73	35.787361499227885	36.5	34.5	39.0	31.0	41.0
74-75	35.45837994802455	36.0	34.0	39.0	31.0	40.0
76-77	34.54175893116076	35.0	33.5	37.0	29.5	39.0
78-79	34.78201521466205	35.0	34.0	37.0	30.0	39.0
80-81	34.583929146205335	35.0	34.0	37.0	30.0	39.0
82-83	34.33042446915083	35.0	34.0	36.5	30.0	38.5
84-85	34.06783757898038	35.0	34.0	36.0	30.0	37.0
86-87	33.85484140620353	35.0	34.0	36.0	30.0	37.0
88-89	33.64928183485063	35.0	33.5	35.0	29.5	36.5
90-91	33.46343981921778	35.0	33.0	35.0	29.0	36.0
92-93	33.32473616775444	35.0	33.0	35.0	29.0	36.0
94-95	33.2692560855932	35.0	33.0	35.0	29.0	36.0
96-97	33.26210741361521	35.0	33.0	35.0	29.0	35.5
98-99	33.34840073940317	35.0	33.0	35.0	30.0	35.0
100-101	31.75016221088419	33.5	30.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	13.0
20	46.0
21	263.0
22	1379.0
23	4501.0
24	11575.0
25	23900.0
26	42103.0
27	67136.0
28	96302.0
29	129078.0
30	166189.0
31	209191.0
32	262847.0
33	341643.0
34	468512.0
35	675153.0
36	1120387.0
37	2162514.0
38	2876609.0
39	1436891.0
40	78706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.44294527113228	21.501068163441932	15.48279248007433	24.573194085351453
2	13.448309346635048	21.598082977411828	41.85121904426426	23.10238863168886
3	16.64360896816701	26.753780735243758	31.60080184964808	25.001808446941148
4	11.673581203075281	18.989790702471886	38.98553318392706	30.351094910525774
5	12.673075942160256	38.106609168544566	36.09946239491708	13.120852494378099
6	29.352897476260548	38.8023576746047	18.66246693715472	13.18227791198003
7	25.78422813459066	33.0971452316038	23.21577098088333	17.902855652922213
8	23.998043428650504	37.36666017359116	21.168228772213148	17.467067625545187
9	24.990508266481683	16.982807160899895	21.386784774659905	36.63989979795851
10-11	23.638446940603348	27.124995565037775	29.269227318030673	19.9673301763282
12-13	24.5355513837598	24.620755804836815	29.298217586803815	21.54547522459957
14-15	21.697265809565355	26.10749132925594	26.829097139409996	25.366145721768714
16-17	21.326794390628937	29.406180627645135	27.45574112863101	21.81128385309492
18-19	21.39311983132676	28.36607038302292	29.247662848511286	20.993146937139038
20-21	22.068164203129616	27.465162001386584	29.235849117799052	21.23082467768475
22-23	21.73495819511365	27.615075304919596	29.074745938627782	21.57522056133897
24-25	21.679242672216642	27.802292953053865	29.13640064816342	21.382063726566074
26-27	21.658346626316586	27.95478969349715	29.059479387171343	21.327384293014923
28-29	21.473762646815754	27.875630128824252	29.13162550592569	21.5189817184343
30-31	21.95767803603192	27.702093843401038	29.048049560208344	21.292178560358703
32-33	21.343605750254532	28.055043693688763	29.079218588672834	21.52213196738387
34-35	21.682312527459736	27.965119487556798	29.12754540360559	21.22502258137787
36-37	21.499245378821623	27.931658777377454	29.05789744848842	21.51119839531251
38-39	21.445879210348558	27.75066205060101	29.20943600304198	21.59402273600846
40-41	21.66521654187778	27.65104286974069	29.135674473558915	21.548066114822614
42-43	21.711283636004	27.695349042700318	28.96330601163773	21.630061309657954
44-45	21.64241112711538	27.94429287660095	28.71130196990499	21.70199402637868
46-47	21.904191258285802	27.735155745200892	28.31507412063673	22.045578875876576
48-49	21.766235558171093	27.868328058200532	28.33637342583635	22.029062957792025
50-51	21.67673501666812	28.004402367381363	28.349694533471403	21.969168082479115
52-53	21.840040967524637	28.009887244504444	28.36760101808684	21.782470769884082
54-55	21.826463638470006	27.98474832711016	28.161614986017874	22.02717304840196
56-57	21.960470017266562	28.207322873624502	27.932671880305566	21.89953522880337
58-59	21.711569532971208	28.382955612588596	28.24275478098923	21.662720073450963
60-61	21.980312226133385	28.514853714891174	27.838011305790516	21.66682275318492
62-63	21.78956974781132	28.687779009637186	27.81517093394713	21.707480308604364
64-65	21.792258762752965	28.488192165426746	27.87571106473464	21.84383800708564
66-67	21.878248881511205	28.37765690722136	27.810947599043946	21.933146612223485
68-69	21.877586568439995	28.26531671867716	27.915579802053013	21.94151691082983
70-71	21.873163803549332	28.050762610139873	28.0078341287114	22.068239457599393
72-73	21.94784360716009	28.048154772022237	27.932779102498305	22.07122251831936
74-75	21.777336051215272	28.20062138528464	27.915012783383762	22.10702978011632
76-77	21.8739280242846	28.12691893192537	27.89015426873412	22.108998775055912
78-79	21.865976381435274	28.088110364147926	27.860665027243275	22.185248227173517
80-81	21.901946212256632	28.09480316271735	27.85064510520761	22.152605519818405
82-83	21.936365374857342	28.057263245639568	27.934531334394837	22.071840045108253
84-85	21.997715938929886	28.02487643151879	27.915901468178106	22.061506161373217
86-87	21.894862547821166	28.050358661135515	27.961415319960565	22.093363471082753
88-89	21.9156054899211	28.015169010958612	27.950169991990915	22.11905550712937
90-91	21.955023629401353	28.02241061551623	27.964481397029182	22.05808435805324
92-93	21.99169846320616	27.930588707947972	28.03412395304057	22.043588875805295
94-95	21.81115956838928	27.907656254454622	28.120235032436742	22.160949144719357
96-97	21.789312941679462	27.958304713153296	28.15766549921189	22.09471684595535
98-99	21.947609926611598	28.485595676549725	28.210831896576288	21.355962500262393
100-101	20.259586124046997	32.141877101476496	26.180649329677543	21.417887444798968
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2763.0
1	2280.5
2	2537.5
3	3474.0
4	4622.0
5	6953.0
6	9027.5
7	11244.0
8	13120.5
9	13528.5
10	12746.0
11	12080.5
12	13806.5
13	18684.5
14	29931.5
15	45322.5
16	57565.5
17	63912.5
18	64393.5
19	60861.0
20	55562.0
21	51052.0
22	49477.0
23	52835.5
24	61705.5
25	76459.0
26	95782.0
27	116555.0
28	138781.5
29	163558.0
30	186806.5
31	209839.0
32	235965.5
33	261759.0
34	286235.0
35	308752.0
36	333018.5
37	351920.5
38	363688.0
39	377820.0
40	391412.5
41	401432.0
42	405571.5
43	409116.5
44	414633.0
45	420649.5
46	430479.5
47	436115.5
48	427245.5
49	415361.5
50	405109.5
51	386011.5
52	362990.0
53	339447.5
54	314563.0
55	290707.5
56	265688.0
57	238328.5
58	211276.5
59	182614.0
60	152096.0
61	123067.5
62	96469.5
63	73119.5
64	53884.0
65	39626.5
66	29644.0
67	22653.0
68	16920.0
69	12068.5
70	8647.5
71	6063.5
72	4088.0
73	2646.0
74	1693.5
75	1065.0
76	693.5
77	459.5
78	303.0
79	210.5
80	149.0
81	95.0
82	69.0
83	48.0
84	31.5
85	23.0
86	17.5
87	14.0
88	11.0
89	8.5
90	6.0
91	5.0
92	4.0
93	2.5
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.828065850399933E-6
2	0.004068819262065573
3	0.004579878686286369
4	9.828065850399933E-6
5	9.828065850399933E-6
6	9.828065850399933E-6
7	9.828065850399933E-6
8	0.0
9	0.00293859168926958
10-11	0.0010712591776935927
12-13	4.9140329251999665E-6
14-15	6.437383132011957E-4
16-17	4.42262963267997E-5
18-19	2.4570164625999834E-5
20-21	1.4742496499271352E-5
22-23	5.012816346184703E-4
24-25	0.0042370180004941875
26-27	0.021196412864821317
28-29	0.3339199578964499
30-31	0.32609447786197626
32-33	0.33681517316106835
34-35	0.17917412709964498
36-37	0.0392495517514311
38-39	0.03615400556690711
40-41	0.013225723201560726
42-43	0.010831172418181333
44-45	0.01002061558976816
46-47	0.006264922410432509
48-49	0.00977951594157487
50-51	0.009579728432828422
52-53	0.0032835649049829
54-55	0.003934486370955227
56-57	0.004013314075171905
58-59	0.0048243380730777895
60-61	0.003361468790492448
62-63	0.0018863127097031869
64-65	5.942790660489603E-4
66-67	0.006681670363139141
68-69	0.015995812506349252
70-71	0.012949981393104692
72-73	0.014227914899364615
74-75	0.007403241849993144
76-77	0.00474632082327658
78-79	0.0015611775562936123
80-81	0.0012120827757038375
82-83	6.380631030780411E-4
84-85	6.89555781270148E-5
86-87	0.0
88-89	5.275031116250621E-4
90-91	0.004622631962104864
92-93	0.017539307492662092
94-95	0.001513281817797356
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	155.0
20-21	618.0
22-23	1276.0
24-25	2819.0
26-27	4827.0
28-29	8467.0
30-31	19521.0
32-33	26239.0
34-35	38571.0
36-37	53685.0
38-39	63606.0
40-41	66862.0
42-43	80360.0
44-45	94788.0
46-47	94800.0
48-49	84439.0
50-51	74771.0
52-53	73611.0
54-55	81113.0
56-57	89131.0
58-59	88999.0
60-61	91963.0
62-63	91716.0
64-65	98412.0
66-67	107990.0
68-69	95632.0
70-71	89925.0
72-73	86637.0
74-75	88209.0
76-77	89449.0
78-79	94381.0
80-81	96821.0
82-83	95236.0
84-85	96590.0
86-87	103557.0
88-89	112006.0
90-91	122289.0
92-93	128516.0
94-95	149094.0
96-97	230034.0
98-99	908286.0
100-101	6149541.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.98768586267086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.45122335131356	76.02252470213045
2	7.389920717008702	12.56104520894358
3	1.7998993299214856	4.58907836507397
4	0.6452925879595213	2.1936769502005475
5	0.2828421990395049	1.2019051980338236
6	0.16256206837164003	0.8289464399972978
7	0.0798478483261074	0.47502586952445836
8	0.048023958018411236	0.3265156046360662
9	0.03638167922397269	0.2782795252539104
>10	0.10156725994914907	1.2989514387673629
>50	0.0019384362638656723	0.1070769198954955
>100	4.266331058836399E-4	0.06798139365428266
>500	5.5450319944425885E-5	0.03023855341905694
>1k	1.8481178216694374E-5	0.01875383046971034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.828065850399933E-6	0.0	0.0	0.0
3	0.0	9.828065850399933E-6	0.0	9.828065850399933E-6	0.0
4	0.0	6.879646095279954E-5	0.0	1.9656131700799866E-5	0.0
5	0.0	9.828065850399934E-5	0.0	1.9656131700799866E-5	0.0
6	0.0	9.828065850399934E-5	0.0	2.94841975511998E-5	0.0
7	0.0	9.828065850399934E-5	0.0	4.914032925199967E-5	0.0
8	0.0	1.2776485605519913E-4	0.0	6.879646095279954E-5	0.0
9	0.0	1.769051853071988E-4	0.0	7.862452680319946E-5	0.0
10-11	0.0	1.769051853071988E-4	0.0	1.0810872435439926E-4	0.0
12-13	0.0	1.769051853071988E-4	0.0	1.2285082312999917E-4	4.9140329251999665E-6
14-15	0.0	2.063893828583986E-4	0.0	1.3759292190559908E-4	9.828065850399933E-6
16-17	0.0	2.2604551455919847E-4	0.0	1.47420987755999E-4	1.9656131700799866E-5
18-19	0.0	2.4570164625999834E-4	0.0	1.621630865315989E-4	2.4570164625999834E-5
20-21	0.0	2.653577779607982E-4	0.0	1.9656131700799867E-4	2.94841975511998E-5
22-23	0.0	2.94841975511998E-4	0.0	2.506156791851983E-4	4.914032925199967E-5
24-25	0.0	3.1449810721279785E-4	0.0	3.0467004136239793E-4	7.862452680319946E-5
26-27	0.0	3.292402059883978E-4	0.0	3.7838053524039746E-4	7.862452680319946E-5
28-29	0.0	3.3415423891359775E-4	0.0	6.584804119767955E-4	7.862452680319946E-5
30-31	0.0	3.7838053524039746E-4	0.0	0.0016658571616427888	7.862452680319946E-5
32-33	0.0	4.0786473279159723E-4	0.0	0.003675696628049575	8.353855972839942E-5
34-35	0.0	4.914032925199967E-4	0.0	0.006545491856366356	8.84525926535994E-5
36-37	0.0	5.700278193231962E-4	0.0	0.011061488114625125	8.84525926535994E-5
38-39	0.0	5.749418522483961E-4	0.0	0.01781828338677508	8.84525926535994E-5
40-41	0.0	6.928786424531953E-4	0.0	0.02461930495525183	8.84525926535994E-5
42-43	0.0	7.371049387799951E-4	0.0	0.031636543972437384	8.84525926535994E-5
44-45	0.0	8.353855972839943E-4	0.0	0.03935157566500133	1.031946914291993E-4
46-47	0.0	9.041820582367939E-4	0.0	0.04807398410723128	1.1302275727959923E-4
48-49	0.0	9.041820582367939E-4	0.0	0.056452410244697215	1.2776485605519913E-4
50-51	0.0	0.0010565170789179929	0.0	0.06537138000393516	1.3267888898039912E-4
52-53	0.0	0.0011007433752447927	0.0	0.07345496416588909	1.5724905360639893E-4
54-55	0.0	0.0011007433752447927	0.0	0.08147957993274066	1.769051853071988E-4
56-57	0.0	0.0011105714410951926	0.0	0.088123352447611	1.8673325115759875E-4
58-59	0.0	0.0011253135398707923	0.0	0.09390225516764616	1.9656131700799867E-4
60-61	0.0	0.001164625803272392	0.0	0.09990720340224052	1.9656131700799867E-4
62-63	0.0	0.0012285082312999916	0.0	0.1054551465747913	2.063893828583986E-4
64-65	0.0	0.00144963971293399	0.0	0.11065419340965285	2.063893828583986E-4
66-67	0.0	0.00147420987755999	0.0	0.11637412773458561	2.063893828583986E-4
68-69	0.0	0.00149386600926079	0.0	0.12212354625706957	2.1130341578359856E-4
70-71	0.0	0.0014987800421859898	0.0	0.12773045782472275	2.1621744870879854E-4
72-73	0.0	0.00150369407511119	0.0	0.13209411906230029	2.358735804095984E-4
74-75	0.0	0.0015430063385127896	0.0	0.13602534540246028	2.358735804095984E-4
76-77	0.0	0.0015921466677647892	0.0	0.13922929486969066	2.358735804095984E-4
78-79	0.0	0.0016019747336151891	0.0	0.14211383219678303	2.358735804095984E-4
80-81	0.0	0.0016019747336151891	0.0	0.14330794219760662	2.4570164625999834E-4
82-83	0.0	0.0016068887665403892	0.0	0.14377968935842583	2.604437450355982E-4
84-85	0.0	0.001611802799465589	0.0	0.14414332779489064	2.702718108859982E-4
86-87	0.0	0.0016412869970167888	0.0	0.14425143651924505	2.800998767363981E-4
88-89	0.0	0.0017248255567451883	0.0	0.14430549088142222	3.0467004136239793E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12770	0.0	50.807587	1
GTATCAA	23165	0.0	36.775833	1
CTTATAC	9490	0.0	35.5496	1
TCAACGC	28570	0.0	29.295881	4
ATCAACG	28805	0.0	29.072767	3
CAACGCA	29360	0.0	28.507605	5
TATACAC	13780	0.0	28.243198	3
TATCAAC	29890	0.0	28.146196	2
AACGCAG	30325	0.0	27.71252	6
TTATACA	14525	0.0	24.659931	2
ACGCAGA	34705	0.0	24.16605	7
CGCAGAG	35190	0.0	23.832985	8
ACATCTC	16105	0.0	21.605968	8
TACACAT	17950	0.0	21.44442	5
ACACATC	16180	0.0	21.295748	6
GCAGAGT	40760	0.0	20.48261	9
GTACATG	28120	0.0	20.09633	1
GTGGTAT	6415	0.0	19.539156	1
GAGTACT	24885	0.0	19.53117	12-13
TACATGG	28520	0.0	19.21855	2
>>END_MODULE