FastQCFastQC Report
Fri 27 May 2016
SRR2139648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8097779
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117400.045.875351
GTATCAA203150.034.6798441
TCAACGC248350.027.852474
ATCAACG250300.027.6378693
CAACGCA254600.027.168745
TATCAAC262650.026.5165522
AACGCAG261950.026.4874176
ACGCAGA304000.022.7817367
CGCAGAG310250.022.3638328
GTACATG232750.019.8210071
GCAGAGT352900.019.3967239
TACATGG235300.019.1011682
GAGTACT209250.018.74880812-13
AGAGTAC311450.018.71437310-11
ACATGGG237650.018.429763
CAGAGTA339150.017.76145210-11
AGTACTT228100.017.12500612-13
GTACTTT233400.016.90018714-15
CATGGGG165550.016.200494
TATACCG5650.015.7728845
Produced by FastQC (version 0.11.5)