##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139648_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8097779
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57361098641986	34.0	31.0	34.0	31.0	34.0
2	32.71322457676358	34.0	31.0	34.0	31.0	34.0
3	32.783843569946775	34.0	31.0	34.0	31.0	34.0
4	36.26678265731876	37.0	37.0	37.0	35.0	37.0
5	36.198453921748175	37.0	37.0	37.0	35.0	37.0
6	36.22602950759708	37.0	37.0	37.0	35.0	37.0
7	36.17378728167316	37.0	37.0	37.0	35.0	37.0
8	36.15182533383536	37.0	37.0	37.0	35.0	37.0
9	37.870109693040526	39.0	38.0	39.0	35.0	39.0
10-11	37.81034756567202	39.0	38.0	39.0	35.0	39.0
12-13	37.77662943135395	39.0	38.0	39.0	35.0	39.0
14-15	39.14923314158116	40.0	39.0	41.0	36.0	41.0
16-17	39.114389575215625	40.0	38.0	41.0	36.0	41.0
18-19	39.003053862052795	40.0	38.5	41.0	35.5	41.0
20-21	39.06690352000996	40.0	39.0	41.0	36.0	41.0
22-23	38.982983963189355	40.0	38.0	41.0	35.0	41.0
24-25	38.87546191666726	40.0	38.0	41.0	35.0	41.0
26-27	38.823353735346814	40.0	38.0	41.0	35.0	41.0
28-29	38.70960262524541	40.0	38.0	41.0	35.0	41.0
30-31	38.64280435876468	40.0	38.0	41.0	35.0	41.0
32-33	38.514105168054364	40.0	38.0	41.0	34.0	41.0
34-35	38.28028096774615	40.0	38.0	41.0	34.0	41.0
36-37	38.54863665470765	40.0	38.0	41.0	34.0	41.0
38-39	38.64365341314044	40.0	38.0	41.0	35.0	41.0
40-41	38.6379132557567	40.0	38.0	41.0	35.0	41.0
42-43	38.56713907211825	40.0	38.0	41.0	34.0	41.0
44-45	38.49306781849438	40.0	38.0	41.0	34.0	41.0
46-47	38.42132346068428	40.0	38.0	41.0	34.0	41.0
48-49	38.354518155313755	40.0	38.0	41.0	34.0	41.0
50-51	38.27809179770622	40.0	38.0	41.0	33.5	41.0
52-53	38.179714795787106	40.0	37.5	41.0	33.0	41.0
54-55	38.0620913069509	40.0	37.0	41.0	33.0	41.0
56-57	37.91334299789712	40.0	37.0	41.0	33.0	41.0
58-59	37.74331207234415	39.5	36.0	41.0	33.0	41.0
60-61	37.575658641364335	39.0	36.0	41.0	33.0	41.0
62-63	37.360233090780554	39.0	35.5	41.0	32.0	41.0
64-65	37.07827288526981	38.5	35.0	40.0	32.0	41.0
66-67	36.75668109049482	38.0	35.0	40.0	31.5	41.0
68-69	36.441220968880245	37.5	35.0	40.0	31.0	41.0
70-71	36.10376397977477	37.0	35.0	39.5	31.0	41.0
72-73	35.787898887874036	36.5	34.5	39.0	31.0	41.0
74-75	35.4602170806922	36.0	34.0	39.0	31.0	40.0
76-77	34.5457920544909	35.0	33.5	37.0	29.5	39.0
78-79	34.78477109777029	35.0	34.0	37.0	30.0	39.0
80-81	34.586436276502056	35.0	34.0	37.0	30.0	39.0
82-83	34.33198183401747	35.0	34.0	36.5	30.0	38.5
84-85	34.071385293590836	35.0	34.0	36.0	30.0	37.0
86-87	33.86062656438714	35.0	34.0	36.0	30.0	37.0
88-89	33.651468785596876	35.0	33.5	35.0	29.5	36.5
90-91	33.46805590658434	35.0	33.0	35.0	29.0	36.0
92-93	33.33349496729296	35.0	33.0	35.0	29.0	36.0
94-95	33.276903408900665	35.0	33.0	35.0	29.0	36.0
96-97	33.270838420607475	35.0	33.0	35.0	29.0	35.5
98-99	33.35384175455299	35.0	33.0	35.0	30.0	35.0
100-101	31.781992735065856	33.5	30.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	7.0
20	38.0
21	203.0
22	1021.0
23	3411.0
24	8661.0
25	17899.0
26	32840.0
27	52112.0
28	75563.0
29	101596.0
30	132336.0
31	166026.0
32	210467.0
33	273486.0
34	375815.0
35	541883.0
36	892644.0
37	1713125.0
38	2273850.0
39	1161096.0
40	63698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.6858592566998	21.509283163850625	15.537521527510387	24.267336051939186
2	13.555001141102514	21.6891972664914	41.74371578544901	23.01208580695707
3	16.896419710548706	26.677645512936888	31.303271794985793	25.122662981528617
4	11.834604505087693	18.76168993518963	39.23384908798438	30.1698564717383
5	12.799338287614207	38.13090455184126	35.91667985958617	13.153077300958364
6	29.486187445494306	38.682006347914204	18.716183624693095	13.115622581898393
7	25.991376893760236	33.051424724165074	23.392281191210724	17.564917190863962
8	23.98334160514877	37.47405307060121	21.074556369098243	17.468048955151776
9	25.070005681960623	17.180762049591007	21.634994356940002	36.114237911508376
10-11	23.80555354881414	27.228807421361978	29.11370567339759	19.851933356426297
12-13	24.627931930471306	24.7369741752646	29.32415172110773	21.31094217315637
14-15	21.710675317984332	26.21999064922671	27.00498263339609	25.064351399392866
16-17	21.22275918721387	29.363617862484716	27.71186804870868	21.701754901592736
18-19	21.29530025222978	28.46229365473849	29.51552741339442	20.72687867963731
20-21	22.038229208905786	27.570554342115116	29.46205183464352	20.929164614335573
22-23	21.743525127387958	27.498347170824545	29.329161933784814	21.428965768002683
24-25	21.543054107047947	27.661688192767958	29.37274070130176	21.422516998882337
26-27	21.529235827019814	27.774567116955666	29.30607086799076	21.390126188033765
28-29	21.49294359740818	27.820495548469626	29.310841468751953	21.37571938537024
30-31	21.730287779196477	27.678578913056857	29.263987633953548	21.32714567379312
32-33	21.467423334653944	27.886193858706765	29.164353111229524	21.482029695409768
34-35	21.667293746549994	27.913208820218742	29.11728321696779	21.302214216263472
36-37	21.596935770845928	27.777446817847355	29.13530005212775	21.490317359178963
38-39	21.468288532793725	27.90981521801537	29.17903859336814	21.44285765582276
40-41	21.73494927095373	27.78661915889906	29.03040641030762	21.448025159839588
42-43	21.650062154127568	27.899745293386424	28.79882309869685	21.651369453789158
44-45	21.732569873073636	28.01217335865503	28.57700834614797	21.67824842212336
46-47	21.825969291038952	27.967924037746812	28.343670578325508	21.862436092888725
48-49	21.765832079053794	28.09698906581715	28.292139835810925	21.845039019318126
50-51	21.755282992787432	28.190380568441313	28.1751597391904	21.879176699580853
52-53	21.90944687407127	28.130482817607383	28.079501068231977	21.880569240089372
54-55	21.90436004868071	28.09772780693666	28.080445750439292	21.917466393943332
56-57	21.910941571098817	28.21306157401534	28.006139373731965	21.86985748115388
58-59	21.84349271032888	28.26505059272901	28.047627918001456	21.84382877894065
60-61	21.94980502794325	28.268137496595617	28.004704030576338	21.777353444884792
62-63	21.928460037869446	28.320085269836916	27.95565230600714	21.795802386286503
64-65	21.865721976834294	28.239916550078203	27.963356071668855	21.931005401418645
66-67	21.928679195383904	28.26174651891301	27.843477882752047	21.96609640295103
68-69	21.905396738464642	28.193177150516323	27.942458330030483	21.95896778098855
70-71	21.889068260743578	28.02143682245989	27.98527027013246	22.104224646664075
72-73	21.979210332462998	28.06982051323958	27.87360142457429	22.077367729723132
74-75	21.817161468895048	28.225671831161513	27.87472591300094	22.082440786942495
76-77	21.952112443919248	28.12949920493682	27.833549831528416	22.08483851961552
78-79	21.916567395003582	28.10537046390093	27.800339173746476	22.177722967349016
80-81	21.92281154203764	28.10886369474553	27.839716529717695	22.128608233499133
82-83	21.989094678865122	28.049372144784822	27.90825701509289	22.053276161257163
84-85	22.02258356116338	28.031950561616302	27.885892788879456	22.059573088340866
86-87	21.925528410486155	28.01152351119709	27.992692991860473	22.07025508645628
88-89	21.9391255128948	27.99334672850457	27.969000949461094	22.09852680913954
90-91	21.955881069221647	28.022205282389333	27.975870985326733	22.046042663062284
92-93	22.03383243352212	27.908807318041056	28.022382782999234	22.03497746543759
94-95	21.846053372230024	27.91464826561012	28.109559955104547	22.12973840705531
96-97	21.842236694962516	27.930919059012727	28.160715771886586	22.06612847413817
98-99	21.952516710624266	28.518323891571363	28.170335956558056	21.358823441246322
100-101	20.316650098919403	32.1293762465518	26.15061839981398	21.40335525471482
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2242.0
1	1881.5
2	2091.0
3	2809.0
4	3688.0
5	5493.5
6	7097.5
7	8887.5
8	10494.0
9	10825.5
10	10090.5
11	9366.5
12	10586.5
13	14677.0
14	23977.5
15	36594.0
16	46492.0
17	51522.0
18	52267.5
19	49887.5
20	45908.0
21	42144.5
22	40807.0
23	43684.0
24	50891.5
25	62197.5
26	77384.5
27	94146.0
28	112116.5
29	132168.5
30	151808.0
31	171016.5
32	191322.0
33	211775.5
34	231116.5
35	248876.5
36	267901.0
37	283820.0
38	294806.0
39	304045.5
40	313346.5
41	321545.5
42	324612.5
43	328279.0
44	332909.0
45	335162.5
46	336381.0
47	334243.5
48	328250.0
49	321118.0
50	312931.0
51	300714.5
52	284375.5
53	267731.5
54	250112.5
55	231625.0
56	212196.5
57	191265.5
58	170332.0
59	146834.0
60	121801.0
61	98646.5
62	78087.5
63	60132.5
64	44360.0
65	32472.5
66	24606.5
67	18776.0
68	14077.0
69	10319.5
70	7391.0
71	5203.5
72	3597.5
73	2456.5
74	1675.5
75	1079.5
76	672.5
77	420.0
78	277.0
79	201.5
80	137.5
81	89.5
82	66.5
83	44.0
84	27.0
85	21.0
86	18.5
87	15.5
88	12.0
89	6.5
90	3.5
91	2.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2349065095503347E-5
2	0.004285125588139662
3	0.0048037863221508026
4	1.2349065095503347E-5
5	1.2349065095503347E-5
6	1.2349065095503347E-5
7	1.2349065095503347E-5
8	0.0
9	0.002741492451201743
10-11	9.138308170672478E-4
12-13	0.0
14-15	6.545004500616774E-4
16-17	3.704719528651004E-5
18-19	4.939626038201339E-5
20-21	6.174745291756716E-6
22-23	6.731072948466226E-4
24-25	0.0040393586709002935
26-27	0.021247230337955766
28-29	0.3320945497954817
30-31	0.3250303779867612
32-33	0.33540874576009627
34-35	0.17795335598914389
36-37	0.03844185753405107
38-39	0.035417115745510486
40-41	0.01311423198608018
42-43	0.009966369401473288
44-45	0.009020588044542402
46-47	0.005889994024841603
48-49	0.009134134002670765
50-51	0.00948809727472958
52-53	0.0030927437018579036
54-55	0.003724326309442487
56-57	0.003567379716789462
58-59	0.00424525794742807
60-61	0.002554516603423343
62-63	0.0012367718689041383
64-65	4.0912189462374333E-4
66-67	0.006707416777779789
68-69	0.016110512931064296
70-71	0.013059045311682558
72-73	0.014162460499322424
74-75	0.007021847168306238
76-77	0.004413481071918013
78-79	0.0014751756827782211
80-81	0.0011928093145017215
82-83	5.063448515361841E-4
84-85	7.885205288954136E-5
86-87	0.0
88-89	4.2100937158764815E-4
90-91	0.004520615816368969
92-93	0.017147464180852598
94-95	0.001429337631328903
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	153.0
20-21	591.0
22-23	1209.0
24-25	2255.0
26-27	4192.0
28-29	6947.0
30-31	16358.0
32-33	22017.0
34-35	31886.0
36-37	45014.0
38-39	53848.0
40-41	56212.0
42-43	66776.0
44-45	79141.0
46-47	78682.0
48-49	70273.0
50-51	62121.0
52-53	60045.0
54-55	64699.0
56-57	67990.0
58-59	67864.0
60-61	67368.0
62-63	66215.0
64-65	72068.0
66-67	75523.0
68-69	74765.0
70-71	73262.0
72-73	70800.0
74-75	71498.0
76-77	73300.0
78-79	77519.0
80-81	79359.0
82-83	77397.0
84-85	78687.0
86-87	84056.0
88-89	90294.0
90-91	99085.0
92-93	103153.0
94-95	118680.0
96-97	182702.0
98-99	719268.0
100-101	4884507.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.16960226099017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.90310667695483	76.57011837703614
2	7.019871232846134	11.957592816497439
3	1.7316542510164674	4.42452911437876
4	0.6153308854238949	2.096299470818242
5	0.28453831597678775	1.2117007599877478
6	0.14497811421055065	0.7408636994316603
7	0.08291582911983292	0.4943335731093478
8	0.04875051988714105	0.33216499110434355
9	0.040542141415601966	0.31076622532580733
>10	0.12613973627614475	1.6691677089275152
>50	0.0017508642705750077	0.09725465588041055
>100	3.740142775327654E-4	0.06296039938991561
>500	4.741832445256246E-5	0.03224820811272148
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.704719528651004E-5	0.0	0.0	1.2349065095503347E-5
3	0.0	3.704719528651004E-5	0.0	0.0	1.2349065095503347E-5
4	0.0	6.174532547751674E-5	0.0	0.0	2.4698130191006694E-5
5	0.0	6.174532547751674E-5	0.0	1.2349065095503347E-5	2.4698130191006694E-5
6	0.0	6.174532547751674E-5	0.0	1.2349065095503347E-5	2.4698130191006694E-5
7	0.0	6.174532547751674E-5	0.0	1.2349065095503347E-5	2.4698130191006694E-5
8	0.0	7.409439057302008E-5	0.0	1.2349065095503347E-5	2.4698130191006694E-5
9	4.939626038201339E-5	1.3583971605053683E-4	0.0	7.409439057302008E-5	2.4698130191006694E-5
10-11	7.409439057302008E-5	1.3583971605053683E-4	0.0	8.644345566852343E-5	2.4698130191006694E-5
12-13	8.026892312077176E-5	1.3583971605053683E-4	0.0	8.644345566852343E-5	2.4698130191006694E-5
14-15	8.644345566852343E-5	2.4698130191006695E-4	0.0	1.2349065095503347E-4	3.087266273875837E-5
16-17	9.261798821627511E-5	2.4698130191006695E-4	0.0	1.3583971605053683E-4	3.704719528651004E-5
18-19	1.1114158585953012E-4	2.593303670055703E-4	0.0	1.4818878114604016E-4	3.704719528651004E-5
20-21	1.4201424859828848E-4	2.84028497196577E-4	0.0	2.0375957407580523E-4	3.704719528651004E-5
22-23	1.605378462415435E-4	2.963775622920803E-4	0.0	2.8402849719657696E-4	4.939626038201339E-5
24-25	1.7288691133704687E-4	3.0872662738758367E-4	0.0	3.272502250308387E-4	4.939626038201339E-5
26-27	1.9758504152805355E-4	3.21075692483087E-4	0.0	3.951700830561071E-4	4.939626038201339E-5
28-29	2.099341066235569E-4	3.21075692483087E-4	0.0	9.014817519717444E-4	4.939626038201339E-5
30-31	2.346322368145636E-4	3.4577382267409374E-4	0.0	0.002136388261522079	4.939626038201339E-5
32-33	2.4698130191006695E-4	3.4577382267409374E-4	0.0	0.00450123422731097	5.557079292976506E-5
34-35	2.4698130191006695E-4	4.013446156038588E-4	0.0	0.007588500501186807	6.174532547751674E-5
36-37	2.4698130191006695E-4	4.569154085336238E-4	0.0	0.012416984953528616	6.174532547751674E-5
38-39	2.902030297443287E-4	4.692644736291272E-4	0.0	0.01962883896930257	6.79198580252684E-5
40-41	2.963775622920803E-4	5.063116689156372E-4	0.0	0.02727908479596689	8.026892312077176E-5
42-43	2.963775622920803E-4	5.124862014633889E-4	0.0	0.03413281592397125	9.261798821627511E-5
44-45	2.963775622920803E-4	5.927551245841606E-4	0.0	0.04288212854413537	9.879252076402678E-5
46-47	3.0872662738758367E-4	6.791985802526841E-4	0.0	0.05244030492805496	1.0496705331177845E-4
48-49	3.1490115993533535E-4	6.791985802526841E-4	0.0	0.061418075252485896	1.2349065095503347E-4
50-51	3.21075692483087E-4	7.162457755391942E-4	0.0	0.07032792571889157	1.2349065095503347E-4
52-53	3.272502250308387E-4	7.285948406346975E-4	0.0	0.07922542712020172	1.2349065095503347E-4
54-55	3.4577382267409374E-4	7.285948406346975E-4	0.0	0.08802413600074785	1.2349065095503347E-4
56-57	3.7047195286510045E-4	7.409439057302008E-4	0.0	0.09447034798060061	1.2349065095503347E-4
58-59	3.8282101796060375E-4	7.471184382779525E-4	0.0	0.10070662585382979	1.2349065095503347E-4
60-61	3.8282101796060375E-4	7.532929708257041E-4	0.0	0.10664652616476691	1.2349065095503347E-4
62-63	3.889955505083554E-4	7.656420359212075E-4	0.0	0.11207394027424063	1.2349065095503347E-4
64-65	4.013446156038588E-4	9.014817519717444E-4	0.0	0.11750135438371434	1.2349065095503347E-4
66-67	4.1369368069936214E-4	9.014817519717444E-4	0.0	0.12299051381866558	1.2349065095503347E-4
68-69	4.3221727834261717E-4	9.385289472582544E-4	0.0	0.12876987628336115	1.2349065095503347E-4
70-71	4.3221727834261717E-4	9.632270774492611E-4	0.0	0.13420346492538263	1.2349065095503347E-4
72-73	4.6308994108137556E-4	9.632270774492611E-4	0.0	0.13879731714090987	1.4818878114604016E-4
74-75	4.8161353872463054E-4	9.879252076402678E-4	0.0	0.14264405091815915	1.4818878114604016E-4
76-77	4.8161353872463054E-4	0.001037321468022281	0.0	0.14595360036375404	1.4818878114604016E-4
78-79	4.877880712723822E-4	0.001055845065665536	0.0	0.1490408666376299	1.4818878114604016E-4
80-81	4.939626038201339E-4	0.001062019598213288	0.0	0.15028812221227572	1.605378462415435E-4
82-83	4.939626038201339E-4	0.001062019598213288	0.0	0.15075121215335713	1.9758504152805355E-4
84-85	5.063116689156372E-4	0.001062019598213288	0.0	0.15106611331329245	1.9758504152805355E-4
86-87	5.063116689156372E-4	0.0010743686633087912	0.0	0.15117108036660423	2.0375957407580523E-4
88-89	5.063116689156372E-4	0.0011978593142638247	0.0	0.151226651159534	2.099341066235569E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11740	0.0	45.87535	1
GTATCAA	20315	0.0	34.679844	1
TCAACGC	24835	0.0	27.85247	4
ATCAACG	25030	0.0	27.637869	3
CAACGCA	25460	0.0	27.16874	5
TATCAAC	26265	0.0	26.516552	2
AACGCAG	26195	0.0	26.487417	6
ACGCAGA	30400	0.0	22.781736	7
CGCAGAG	31025	0.0	22.363832	8
GTACATG	23275	0.0	19.821007	1
GCAGAGT	35290	0.0	19.396723	9
TACATGG	23530	0.0	19.101168	2
GAGTACT	20925	0.0	18.748808	12-13
AGAGTAC	31145	0.0	18.714373	10-11
ACATGGG	23765	0.0	18.42976	3
CAGAGTA	33915	0.0	17.761452	10-11
AGTACTT	22810	0.0	17.125006	12-13
GTACTTT	23340	0.0	16.900187	14-15
CATGGGG	16555	0.0	16.20049	4
TATACCG	565	0.0	15.772884	5
>>END_MODULE