FastQCFastQC Report
Fri 27 May 2016
SRR2139647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8633654
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161250.036.9214821
GTATCAA257950.030.7959421
TCAACGC310300.025.1446274
ATCAACG310950.025.0797823
CAACGCA315350.024.7690455
TATCAAC320200.024.5554872
AACGCAG323900.024.1943266
ACGCAGA368450.021.2689447
CGCAGAG375950.020.7992028
GTACATG258450.019.4663641
GAGTACT221800.019.2632212-13
TACATGG258000.019.0037042
AGAGTAC343200.018.7330310-11
GCAGAGT421800.018.4876869
ACATGGG259250.018.3353823
GTACTTT243550.017.57015214-15
AGTACTT240500.017.21496212-13
CAGAGTA404700.016.71989810-11
CATGGGG180400.016.0975864
CTTATAC52900.015.8240071
Produced by FastQC (version 0.11.5)