##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139647_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8633654
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56155840852552	34.0	31.0	34.0	31.0	34.0
2	32.70437684901433	34.0	31.0	34.0	31.0	34.0
3	32.77262987374755	34.0	31.0	34.0	31.0	34.0
4	36.26399783915362	37.0	37.0	37.0	35.0	37.0
5	36.19261218946231	37.0	35.0	37.0	35.0	37.0
6	36.22146544209439	37.0	37.0	37.0	35.0	37.0
7	36.169582542918675	37.0	37.0	37.0	35.0	37.0
8	36.14581867654182	37.0	36.0	37.0	35.0	37.0
9	37.86225160285552	39.0	38.0	39.0	35.0	39.0
10-11	37.796571648574286	39.0	38.0	39.0	35.0	39.0
12-13	37.76432313595147	39.0	38.0	39.0	35.0	39.0
14-15	39.12929844072973	40.0	38.5	41.0	36.0	41.0
16-17	39.09094278042646	40.0	38.0	41.0	36.0	41.0
18-19	38.978665232588654	40.0	38.5	41.0	35.0	41.0
20-21	39.03781491733034	40.0	39.0	41.0	36.0	41.0
22-23	38.949153431140964	40.0	38.0	41.0	35.0	41.0
24-25	38.83794530143907	40.0	38.0	41.0	35.0	41.0
26-27	38.784075611106886	40.0	38.0	41.0	35.0	41.0
28-29	38.6758294620845	40.0	38.0	41.0	35.0	41.0
30-31	38.606800083525755	40.0	38.0	41.0	35.0	41.0
32-33	38.473079345803455	40.0	38.0	41.0	34.0	41.0
34-35	38.24055353348356	40.0	38.0	41.0	34.0	41.0
36-37	38.50902827057381	40.0	38.0	41.0	34.0	41.0
38-39	38.601990551445795	40.0	38.0	41.0	34.5	41.0
40-41	38.59699958442944	40.0	38.0	41.0	34.0	41.0
42-43	38.52647583820905	40.0	38.0	41.0	34.0	41.0
44-45	38.44776578490119	40.0	38.0	41.0	34.0	41.0
46-47	38.37254596357858	40.0	38.0	41.0	34.0	41.0
48-49	38.30125612628419	40.0	38.0	41.0	33.5	41.0
50-51	38.21755989470188	40.0	38.0	41.0	33.0	41.0
52-53	38.119365510134216	40.0	37.0	41.0	33.0	41.0
54-55	37.99082167936163	40.0	37.0	41.0	33.0	41.0
56-57	37.839409621585745	40.0	37.0	41.0	33.0	41.0
58-59	37.66693135338512	39.5	36.0	41.0	33.0	41.0
60-61	37.493194407546895	39.0	36.0	41.0	32.5	41.0
62-63	37.26977616042093	39.0	35.5	40.5	32.0	41.0
64-65	36.97798634562579	38.5	35.0	40.0	32.0	41.0
66-67	36.648412450343926	38.0	35.0	40.0	31.0	41.0
68-69	36.32691960297049	37.0	35.0	40.0	31.0	41.0
70-71	35.98051277678547	37.0	35.0	39.0	31.0	41.0
72-73	35.657168506264796	36.0	34.0	39.0	31.0	40.5
74-75	35.32579283854373	36.0	34.0	39.0	30.0	40.0
76-77	34.409566027149	35.0	33.5	37.0	29.5	39.0
78-79	34.66205380889884	35.0	34.0	37.0	30.0	39.0
80-81	34.47033363974015	35.0	34.0	37.0	30.0	39.0
82-83	34.222585862832915	35.0	34.0	36.0	30.0	38.5
84-85	33.963963898594116	35.0	34.0	36.0	30.0	37.0
86-87	33.75778620631192	35.0	33.5	35.5	30.0	37.0
88-89	33.55733931423879	35.0	33.0	35.0	29.0	36.5
90-91	33.3783850830958	35.0	33.0	35.0	29.0	36.0
92-93	33.2451953019892	35.0	33.0	35.0	29.0	36.0
94-95	33.19413185038303	35.0	33.0	35.0	29.0	36.0
96-97	33.19123766024516	35.0	33.0	35.0	29.0	35.0
98-99	33.28309240935502	34.0	33.0	35.0	29.5	35.0
100-101	31.68911544118866	33.0	30.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	5.0
20	36.0
21	227.0
22	1101.0
23	3745.0
24	9675.0
25	20564.0
26	36205.0
27	57519.0
28	83005.0
29	111423.0
30	144003.0
31	182099.0
32	230955.0
33	298155.0
34	412592.0
35	595391.0
36	991474.0
37	1893402.0
38	2400418.0
39	1103762.0
40	57894.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.5039930967783	21.44544891210554	15.83207565745658	24.218482333659576
2	13.644452804819654	21.579733307634445	41.390436446335	23.3853774412109
3	17.01784896431435	26.32755248390577	31.313480095139496	25.341118456640388
4	11.993004117609587	18.79785490493592	38.77819925523967	30.430941722214822
5	12.952366612035465	37.926809634395646	35.73654248203251	13.384281271536372
6	29.710192097201066	38.400132041488824	18.505209268385908	13.384466592924198
7	26.2197332530274	32.946459492798525	22.976840617815178	17.856966636358898
8	24.337864361949183	37.00101949881244	20.82150848296677	17.839607656271607
9	25.09161215265306	17.200011536581098	21.491492292671943	36.2168840180939
10-11	23.954912033756614	27.150474007413507	28.734182016877728	20.160431941952154
12-13	24.82280387886751	24.637407290123043	28.9856994500822	21.55408938092724
14-15	21.94726176901021	26.087523317370476	26.768312352284713	25.1969025613346
16-17	21.361267065860634	29.312628295283076	27.456703554320466	21.869401084535824
18-19	21.434013175074302	28.349302725503804	29.282065647426236	20.93461845199566
20-21	22.197152046289485	27.35875178516171	29.282327780796763	21.161768387752037
22-23	21.918750033304672	27.310956299402417	29.136950209338703	21.63334345795421
24-25	21.765055909481152	27.48056917484988	29.190126069711408	21.56424884595756
26-27	21.690505790854086	27.578194846844706	29.187228495750528	21.544070866550676
28-29	21.558763261372903	27.630947348062882	29.189263758668933	21.62102563189528
30-31	21.79245963212871	27.50843861976457	29.14607073473038	21.553031013376344
32-33	21.6189080912957	27.696087013561204	29.040360536509553	21.644644358633546
34-35	21.791399875602345	27.70540073491135	29.025925214876914	21.47727417460939
36-37	21.805402111404533	27.567635923467996	29.004675622741804	21.622286342385664
38-39	21.596400583218113	27.707141359727327	29.087514515676933	21.608943541377627
40-41	21.826035437344196	27.638188037663163	28.93069433377333	21.605082191219303
42-43	21.793439147229403	27.72103780536474	28.66165149161144	21.82387155579442
44-45	21.872450678694502	27.79763414564038	28.494146460617465	21.835768715047653
46-47	22.007045481840986	27.80793441865667	28.183311735916234	22.00170836358611
48-49	21.921470089591523	27.878332087465957	28.153119541797007	22.047078281145517
50-51	21.858551698576683	27.99969375084889	28.097822009921337	22.043932540653092
52-53	22.064644347648702	27.97566553955022	27.943164895419947	22.016525217381126
54-55	22.014991978662955	27.880747574312302	27.97105896074732	22.133201486277425
56-57	22.058083902202107	28.02452947182396	27.83416488462048	22.083221741353455
58-59	21.986362592074443	28.070052797661184	27.938859111796617	22.00472549846776
60-61	22.140647429618767	28.115756327066528	27.82055713082054	21.923039112494163
62-63	22.075356137133255	28.201087448167232	27.814920438836587	21.90863597586292
64-65	22.02687253319847	28.131815305989292	27.783921854071615	22.05739030674062
66-67	22.11185590483311	28.074692283773327	27.66618951137455	22.147262300019005
68-69	22.045075555800263	28.047342884642063	27.761187168356717	22.146394391200957
70-71	22.066700545734683	27.830187437552485	27.838800073517895	22.264311943194944
72-73	22.117959131113338	27.863481702366048	27.78209404797134	22.236465118549276
74-75	21.97227723971803	28.02005782670945	27.746529492234462	22.261135441338066
76-77	22.07271407322726	27.931743286666656	27.73890162585853	22.256641014247556
78-79	22.071572243026775	27.87518185876296	27.67791443362017	22.37533146459009
80-81	22.04804422469988	27.93170071942773	27.691569176028025	22.328685879844365
82-83	22.114936212875953	27.88965956706171	27.76187674679195	22.2335274732704
84-85	22.148553804840148	27.859512407814325	27.75169366816496	22.240240119180566
86-87	22.071390956012145	27.860275686223	27.844519199157947	22.223814158606906
88-89	22.073444021905598	27.82295014266378	27.813058747199896	22.290547088230724
90-91	22.093358107609	27.839603611589357	27.82680735727801	22.24023092352363
92-93	22.17108875572864	27.746971742423483	27.873539008670644	22.208400493177226
94-95	21.977880092859678	27.697798553326308	27.98038667386954	22.343934679944475
96-97	21.95516221333058	27.76563743174461	28.03512479748578	22.24407555743903
98-99	22.093741698155206	28.350192056149815	28.016899299141034	21.53916694655394
100-101	20.476330871196723	31.947970916684703	25.964352075413665	21.611346136704903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2234.0
1	1869.0
2	2106.5
3	2860.5
4	3824.0
5	5643.0
6	7190.0
7	9092.5
8	10899.5
9	11353.5
10	10681.5
11	10228.0
12	11645.5
13	15841.0
14	25983.0
15	39753.0
16	50343.0
17	55842.5
18	56511.5
19	53294.0
20	48217.0
21	43048.5
22	40196.5
23	42009.0
24	49044.5
25	60691.5
26	75153.0
27	91566.5
28	109982.0
29	129101.0
30	148077.5
31	167179.0
32	187850.0
33	209706.5
34	230320.5
35	249430.5
36	270608.5
37	288167.5
38	301063.5
39	314280.0
40	326218.0
41	336663.5
42	342194.0
43	347771.0
44	354342.5
45	359459.5
46	364494.5
47	368749.0
48	366872.5
49	356649.5
50	345231.5
51	330945.0
52	313407.5
53	294084.0
54	272957.5
55	252472.5
56	230938.5
57	207037.0
58	182542.5
59	157270.0
60	131442.0
61	107126.5
62	84752.0
63	64772.0
64	47931.0
65	35483.0
66	26744.0
67	20458.0
68	15585.5
69	11127.0
70	7772.0
71	5440.0
72	3744.5
73	2521.5
74	1609.0
75	1036.0
76	708.5
77	460.5
78	292.5
79	195.0
80	141.5
81	102.5
82	77.0
83	54.5
84	38.0
85	30.5
86	26.0
87	21.0
88	12.5
89	5.5
90	3.0
91	2.5
92	3.0
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.633032549138522E-5
2	0.003891747341276359
3	0.005050005478560989
4	4.633032549138522E-5
5	4.633032549138522E-5
6	4.633032549138522E-5
7	4.633032549138522E-5
8	0.0
9	0.0028608975990930376
10-11	0.0010018932887512055
12-13	0.0
14-15	7.991981147263951E-4
16-17	7.528677892350099E-5
18-19	2.8956453432115767E-5
20-21	5.791513394264687E-6
22-23	6.255447452156253E-4
24-25	0.004385455720105733
26-27	0.021992570973271813
28-29	0.3363535411816213
30-31	0.32887104673405304
32-33	0.3401277655773439
34-35	0.17971185800249645
36-37	0.03792899046587667
38-39	0.03480195380711712
40-41	0.012757635835950309
42-43	0.010160994518071901
44-45	0.008953205521163448
46-47	0.005858950354168995
48-49	0.008836838561561235
50-51	0.009119905627513148
52-53	0.002882084761926104
54-55	0.003436970538765202
56-57	0.0035982178134673767
58-59	0.00415205130787384
60-61	0.0028500646604398866
62-63	0.0014341453126647686
64-65	4.5832103778025427E-4
66-67	0.006546514106878258
68-69	0.01595401822125257
70-71	0.012836309451701293
72-73	0.013884214241400477
74-75	0.007055233599816964
76-77	0.004401536073508573
78-79	0.0013776057394991124
80-81	0.0011588402412179282
82-83	4.962554648335037E-4
84-85	8.73202231235448E-5
86-87	0.0
88-89	3.8072396079528607E-4
90-91	0.004303114083357532
92-93	0.017293636711665027
94-95	0.0015365056897354442
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	188.0
20-21	726.0
22-23	1281.0
24-25	2658.0
26-27	4845.0
28-29	6878.0
30-31	15571.0
32-33	21074.0
34-35	30693.0
36-37	43133.0
38-39	51402.0
40-41	54811.0
42-43	65852.0
44-45	79036.0
46-47	78542.0
48-49	69157.0
50-51	60855.0
52-53	59053.0
54-55	64124.0
56-57	67527.0
58-59	67545.0
60-61	67230.0
62-63	67252.0
64-65	72862.0
66-67	78181.0
68-69	75809.0
70-71	74010.0
72-73	71305.0
74-75	73020.0
76-77	75097.0
78-79	80786.0
80-81	81081.0
82-83	80533.0
84-85	82433.0
86-87	88130.0
88-89	95492.0
90-91	105019.0
92-93	110761.0
94-95	130277.0
96-97	200229.0
98-99	794915.0
100-101	5284281.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.76619256302422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.64325412519614	78.64770041965907
2	6.73624080863988	11.689559343066996
3	1.5086003263604824	3.9268651925290436
4	0.5450162071268184	1.8915592471013847
5	0.22361735177701525	0.9701213102359015
6	0.11335501365860713	0.5901229765852168
7	0.07075171119031991	0.4297199618111941
8	0.04188801304465232	0.2907570724731819
9	0.025950307486717297	0.20264484388159815
>10	0.0894045461454324	1.1893815851739482
>50	0.001555778289368422	0.08724751765215376
>100	3.2520724146256776E-4	0.057034597227282
>500	4.0603843079754505E-5	0.02728593260302625
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1582581372846307E-5	1.1582581372846307E-5
2	0.0	1.1582581372846307E-5	0.0	1.1582581372846307E-5	1.1582581372846307E-5
3	0.0	1.1582581372846307E-5	0.0	1.1582581372846307E-5	1.1582581372846307E-5
4	0.0	5.7912906864231527E-5	0.0	1.1582581372846307E-5	3.474774411853892E-5
5	0.0	5.7912906864231527E-5	0.0	1.1582581372846307E-5	3.474774411853892E-5
6	2.3165162745692613E-5	5.7912906864231527E-5	0.0	1.1582581372846307E-5	3.474774411853892E-5
7	2.3165162745692613E-5	5.7912906864231527E-5	0.0	1.1582581372846307E-5	3.474774411853892E-5
8	2.3165162745692613E-5	5.7912906864231527E-5	0.0	3.474774411853892E-5	3.474774411853892E-5
9	2.3165162745692613E-5	9.266065098277045E-5	0.0	8.107806960992415E-5	4.633032549138523E-5
10-11	2.8956453432115767E-5	9.266065098277045E-5	0.0	1.100345230420399E-4	5.7912906864231527E-5
12-13	3.474774411853892E-5	9.84519416691936E-5	0.0	1.2740839510130936E-4	5.7912906864231527E-5
14-15	4.053903480496207E-5	1.737387205926946E-4	0.0	1.2740839510130936E-4	6.370419755065468E-5
16-17	7.528677892350099E-5	1.7953001127911773E-4	0.0	1.3899097647415568E-4	6.949548823707784E-5
18-19	8.107806960992415E-5	2.316516274569261E-4	0.0	1.737387205926946E-4	6.949548823707784E-5
20-21	8.107806960992415E-5	2.7798195294831136E-4	0.0	2.4323420882977243E-4	6.949548823707784E-5
22-23	9.266065098277045E-5	3.185209877532734E-4	0.0	2.7798195294831136E-4	8.107806960992415E-5
24-25	9.84519416691936E-5	3.6485131324465864E-4	0.0	3.0114711569400395E-4	9.266065098277045E-5
26-27	1.0424323235561675E-4	3.938077666767744E-4	0.0	3.995990573631976E-4	9.266065098277045E-5
28-29	1.0424323235561675E-4	3.938077666767744E-4	0.0	7.007461730572015E-4	9.84519416691936E-5
30-31	1.0424323235561675E-4	4.16972929422467E-4	0.0	0.001407283636800826	1.1582581372846305E-4
32-33	1.1582581372846305E-4	4.16972929422467E-4	0.0	0.002762445657423844	1.5057355784700197E-4
34-35	1.2161710441488621E-4	4.92259708345968E-4	0.0	0.004661989002570638	1.621561392198483E-4
36-37	1.2740839510130936E-4	5.675464872694691E-4	0.0	0.007459182404113021	1.621561392198483E-4
38-39	1.2740839510130936E-4	5.791290686423153E-4	0.0	0.011524668465982074	1.6794742990627144E-4
40-41	1.2740839510130936E-4	6.486245568793932E-4	0.0	0.01634881360777256	1.737387205926946E-4
42-43	1.2740839510130936E-4	6.717897196250858E-4	0.0	0.020790733564259117	1.737387205926946E-4
44-45	1.2740839510130936E-4	7.991981147263951E-4	0.0	0.02619400777469192	1.737387205926946E-4
46-47	1.2740839510130936E-4	9.150239284548582E-4	0.0	0.032616549145935196	2.0269517402481035E-4
48-49	1.2740839510130936E-4	9.266065098277045E-4	0.0	0.03859895242501031	2.200690460840798E-4
50-51	1.331996857877325E-4	0.0011061365211068224	0.0	0.044645059901636086	2.200690460840798E-4
52-53	1.3899097647415568E-4	0.0011177191024796685	0.0	0.05061588059933836	2.2586033677050296E-4
54-55	1.3899097647415568E-4	0.0011466755559117843	0.0	0.05679518776175187	2.316516274569261E-4
56-57	1.3899097647415568E-4	0.001169840718657477	0.0	0.06308452944720741	2.316516274569261E-4
58-59	1.3899097647415568E-4	0.001181423300030323	0.0	0.06825036073949686	2.3744291814334925E-4
60-61	1.4478226716057883E-4	0.0012219623348352854	0.0	0.07371733914748031	2.4323420882977243E-4
62-63	1.5057355784700197E-4	0.0012393362068945548	0.0	0.07915536110203165	2.548167902026187E-4
64-65	1.5057355784700197E-4	0.0013319968578773252	0.0	0.0846455046727608	2.548167902026187E-4
66-67	1.5057355784700197E-4	0.0013319968578773252	0.0	0.09050629084742104	2.548167902026187E-4
68-69	1.5057355784700197E-4	0.0013551620206230176	0.0	0.09656398090541965	2.548167902026187E-4
70-71	1.5057355784700197E-4	0.0013899097647415567	0.0	0.10201937673203026	2.7798195294831136E-4
72-73	1.5057355784700197E-4	0.001401492346114403	0.0	0.10621227118900062	2.7798195294831136E-4
74-75	1.5057355784700197E-4	0.0014304487995465188	0.0	0.10976812367046444	2.7798195294831136E-4
76-77	1.5636484853342515E-4	0.0014883617064107503	0.0	0.11304599419897995	2.7798195294831136E-4
78-79	1.621561392198483E-4	0.0015404833225885588	0.0	0.11572736178679387	2.7798195294831136E-4
80-81	1.621561392198483E-4	0.0015404833225885588	0.0	0.11692615895888346	2.8956453432115765E-4
82-83	1.621561392198483E-4	0.0015462746132749818	0.0	0.11731996672556023	3.4168615049896605E-4
84-85	1.6794742990627144E-4	0.0015694397760206745	0.0	0.1176384877133135	3.474774411853892E-4
86-87	1.737387205926946E-4	0.0015752310667070976	0.0	0.11775431352704196	3.474774411853892E-4
88-89	1.737387205926946E-4	0.001627352682884906	0.0	0.11781801772459262	3.474774411853892E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16125	0.0	36.921482	1
GTATCAA	25795	0.0	30.795942	1
TCAACGC	31030	0.0	25.144627	4
ATCAACG	31095	0.0	25.079782	3
CAACGCA	31535	0.0	24.769045	5
TATCAAC	32020	0.0	24.555487	2
AACGCAG	32390	0.0	24.194326	6
ACGCAGA	36845	0.0	21.268944	7
CGCAGAG	37595	0.0	20.799202	8
GTACATG	25845	0.0	19.466364	1
GAGTACT	22180	0.0	19.26322	12-13
TACATGG	25800	0.0	19.003704	2
AGAGTAC	34320	0.0	18.73303	10-11
GCAGAGT	42180	0.0	18.487686	9
ACATGGG	25925	0.0	18.335382	3
GTACTTT	24355	0.0	17.570152	14-15
AGTACTT	24050	0.0	17.214962	12-13
CAGAGTA	40470	0.0	16.719898	10-11
CATGGGG	18040	0.0	16.097586	4
CTTATAC	5290	0.0	15.824007	1
>>END_MODULE