FastQCFastQC Report
Fri 27 May 2016
SRR2139646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9978154
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117000.050.7530671
GTATCAA221200.037.5062641
TCAACGC269700.030.0217784
ATCAACG272150.029.814453
CAACGCA276250.029.3253025
TATCAAC285400.028.623482
AACGCAG283750.028.5501846
ACGCAGA328550.024.6442647
CGCAGAG336700.024.0981258
GTACATG277900.020.696231
GCAGAGT391350.020.4731469
TACATGG277450.020.011292
ACATGGG278850.019.515343
GAGTACT236350.019.06605312-13
AGAGTAC356650.018.77143510-11
CAGAGTA382200.017.99928710-11
GTGGTAT61200.017.7422491
CATGGGG196100.017.4544984
TGGTATC58450.017.2708052
AGTACTT258600.017.22879812-13
Produced by FastQC (version 0.11.5)