##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139646_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9978154
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5749740883935	34.0	31.0	34.0	31.0	34.0
2	32.711536322249586	34.0	31.0	34.0	31.0	34.0
3	32.78421529673725	34.0	31.0	34.0	31.0	34.0
4	36.2729816557251	37.0	37.0	37.0	35.0	37.0
5	36.20303845781494	37.0	37.0	37.0	35.0	37.0
6	36.22954155648429	37.0	37.0	37.0	35.0	37.0
7	36.17637400665494	37.0	37.0	37.0	35.0	37.0
8	36.15319045987865	37.0	37.0	37.0	35.0	37.0
9	37.87136929335827	39.0	38.0	39.0	35.0	39.0
10-11	37.806893138750915	39.0	38.0	39.0	35.0	39.0
12-13	37.77807718742365	39.0	38.0	39.0	35.0	39.0
14-15	39.15184652391615	40.0	39.0	41.0	36.0	41.0
16-17	39.11365293620443	40.0	38.0	41.0	36.0	41.0
18-19	38.9971411044568	40.0	38.5	41.0	35.5	41.0
20-21	39.05657226699192	40.0	39.0	41.0	36.0	41.0
22-23	38.972378235287756	40.0	38.0	41.0	35.0	41.0
24-25	38.864939407702394	40.0	38.0	41.0	35.0	41.0
26-27	38.81395860561865	40.0	38.0	41.0	35.0	41.0
28-29	38.70144324246243	40.0	38.0	41.0	35.0	41.0
30-31	38.63563918404749	40.0	38.0	41.0	35.0	41.0
32-33	38.50836223619153	40.0	38.0	41.0	34.0	41.0
34-35	38.28055035324435	40.0	38.0	41.0	34.0	41.0
36-37	38.55574533661735	40.0	38.0	41.0	34.5	41.0
38-39	38.652401331574296	40.0	38.0	41.0	35.0	41.0
40-41	38.649026943152926	40.0	38.0	41.0	35.0	41.0
42-43	38.57845333268588	40.0	38.0	41.0	34.0	41.0
44-45	38.505643026833	40.0	38.0	41.0	34.0	41.0
46-47	38.436194792575805	40.0	38.0	41.0	34.0	41.0
48-49	38.3684101989363	40.0	38.0	41.0	34.0	41.0
50-51	38.28758332007517	40.0	38.0	41.0	33.5	41.0
52-53	38.19132461977247	40.0	37.5	41.0	33.0	41.0
54-55	38.06907959896008	40.0	37.0	41.0	33.0	41.0
56-57	37.92022220233108	40.0	37.0	41.0	33.0	41.0
58-59	37.750261668959396	39.5	36.5	41.0	33.0	41.0
60-61	37.578388788133076	39.0	36.0	41.0	33.0	41.0
62-63	37.35778071031897	39.0	35.5	41.0	32.0	41.0
64-65	37.07123253978003	38.5	35.0	40.0	32.0	41.0
66-67	36.74548134817171	38.0	35.0	40.0	31.5	41.0
68-69	36.42474437128835	37.5	35.0	40.0	31.0	41.0
70-71	36.083180326361656	37.0	35.0	39.5	31.0	41.0
72-73	35.76277212206226	36.0	34.5	39.0	31.0	41.0
74-75	35.42859157987615	36.0	34.0	39.0	31.0	40.0
76-77	34.50255264516436	35.0	33.5	37.0	29.5	39.0
78-79	34.74953631805572	35.0	34.0	37.0	30.0	39.0
80-81	34.555114554965506	35.0	34.0	37.0	30.0	39.0
82-83	34.30264987004378	35.0	34.0	36.0	30.0	38.5
84-85	34.042122274857775	35.0	34.0	36.0	30.0	37.0
86-87	33.83446051944492	35.0	34.0	35.5	30.0	37.0
88-89	33.633191637673036	35.0	33.5	35.0	30.0	36.5
90-91	33.453154712447706	35.0	33.0	35.0	29.0	36.0
92-93	33.31880965344803	35.0	33.0	35.0	29.0	36.0
94-95	33.26369886992532	35.0	33.0	35.0	29.0	36.0
96-97	33.25800451628562	35.0	33.0	35.0	29.0	35.0
98-99	33.340348215601175	35.0	33.0	35.0	30.0	35.0
100-101	31.756516282469384	33.5	30.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	37.0
21	248.0
22	1053.0
23	3964.0
24	10016.0
25	21330.0
26	38700.0
27	62266.0
28	90082.0
29	123027.0
30	159792.0
31	204170.0
32	257801.0
33	336558.0
34	464882.0
35	669334.0
36	1114790.0
37	2158942.0
38	2813300.0
39	1372642.0
40	75210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.67747641822618	21.443000812475177	15.425182481203182	24.454340288095462
2	13.644898591206278	21.760508574884856	41.443751731989934	23.15084110191893
3	16.80727518147213	26.60130292513217	31.419846832482385	25.17157506091332
4	11.888655523453192	18.764237983570304	39.17977388796782	30.167332605008685
5	12.846598533135047	38.08450082585441	35.94161884301009	13.127281798000451
6	29.534650257347277	38.67089203200072	18.73200756332511	13.062450147326896
7	26.04920490780306	33.044108071725915	23.35812516767886	17.548561852792165
8	24.168308085844338	37.114901213190336	21.162832323493905	17.553958377471425
9	25.277900438310684	16.865149560216057	21.583989904041488	36.27296009743177
10-11	23.782706120422695	27.106348343104703	29.174709533516314	19.93623600295629
12-13	24.613480609740037	24.722889624674064	29.236900933779935	21.426728831805963
14-15	21.785039486001985	26.126370038844094	26.950931405429873	25.137659069724045
16-17	21.325482244979394	29.22440177910744	27.636857916390206	21.813258059522962
18-19	21.40988846530785	28.356045122989222	29.387603975927	20.84646243577593
20-21	22.158799925515492	27.512609349008223	29.317706085660788	21.010884639815497
22-23	21.85284560505315	27.462759869060672	29.133238583401656	21.55115594248452
24-25	21.64285580378567	27.626477341342483	29.209476077473834	21.521190777398015
26-27	21.619945778169132	27.73135364155327	29.138860454889056	21.50984012538855
28-29	21.57884955715685	27.761277557959414	29.14322885442467	21.516644030459066
30-31	21.83137365974365	27.629853202226755	29.099715470380367	21.439057667649227
32-33	21.60908429792134	27.811501323152132	28.99036927054926	21.58904510837727
34-35	21.72326362710628	27.82966302355461	29.019815632338357	21.427257717000753
36-37	21.766374686584605	27.689763386590283	28.96032303085874	21.583538895966374
38-39	21.59155230475904	27.825871242666526	29.021799634999557	21.56077681757487
40-41	21.845363631523643	27.742006399778045	28.8677561804393	21.544873788259007
42-43	21.784143869334265	27.81378846046238	28.662337110257436	21.73973055994592
44-45	21.843610643938494	27.94547813778246	28.45554506417345	21.7553661541056
46-47	21.959377284955238	27.875307274585786	28.16406499170497	22.001250448754007
48-49	21.881153411537607	27.998158262497004	28.164645114135332	21.956043211830053
50-51	21.86880634779995	28.113001435396523	28.034576706270947	21.983615510532577
52-53	22.026934383549722	28.027827709310348	27.923025187205567	22.022212719934362
54-55	22.02218705736423	27.938921327991117	27.959128805991938	22.07976280865271
56-57	22.052997934917133	28.11442744212537	27.846938026081393	21.98563659687611
58-59	21.9710874136962	28.151976107168487	27.920636931580624	21.956299547554696
60-61	22.069159831694183	28.160943673060245	27.84232759274986	21.927568902495707
62-63	22.051306808240202	28.22274487299088	27.780571216946758	21.94537710182216
64-65	22.02423997074443	28.13929281339092	27.779164833997978	22.057302381866673
66-67	22.085148028274222	28.153301311079566	27.661615390251626	22.09993527039458
68-69	22.04211475224439	28.080491496271055	27.775212638542374	22.102181112942183
70-71	22.01227138795136	27.89531809784087	27.840616086647795	22.251794427559975
72-73	22.12156106328446	27.91441126249627	27.74709019770108	22.21693747651819
74-75	21.951280649066266	28.050458879064983	27.75243180596723	22.245828665901517
76-77	22.066319550980765	27.981224826214156	27.711073827470766	22.241381795334313
78-79	22.062183119797073	27.932503752242173	27.695019288507915	22.31029383945284
80-81	22.03522978045679	27.94453213309056	27.708485749597834	22.311752336854816
82-83	22.129675256526234	27.910515109158602	27.75829576132533	22.201513872989835
84-85	22.193421219915997	27.86248855662377	27.738449058118537	22.205641165341696
86-87	22.03653124287915	27.868638848161197	27.81515371422232	22.279676194737334
88-89	22.10292303756399	27.818109691340787	27.82670777141915	22.25225949967607
90-91	22.15309798531196	27.881131826525774	27.784791900675206	22.180978287487065
92-93	22.16951428354063	27.77472694602124	27.853288699097668	22.202470071340468
94-95	22.017898853661528	27.77447441614748	27.898190525441457	22.309436204749534
96-97	21.97108269410179	27.8308094983475	27.956456813730902	22.241650993819814
98-99	22.131595661746598	28.345475183923945	27.999555582714393	21.523373571615068
100-101	20.555651244383515	31.96474207098029	25.871676009938927	21.60793067469727
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2096.0
1	1843.5
2	2403.0
3	3426.5
4	4550.0
5	6700.5
6	8563.0
7	10553.5
8	12249.0
9	12603.0
10	11984.0
11	11321.5
12	12753.5
13	17414.5
14	28454.5
15	43628.5
16	55749.5
17	62016.5
18	62432.0
19	58852.5
20	53378.0
21	48311.0
22	46176.5
23	49207.5
24	57885.5
25	72274.0
26	91177.5
27	111265.5
28	132531.5
29	155806.5
30	177889.0
31	199949.5
32	225458.0
33	252073.0
34	277441.0
35	300787.0
36	326411.0
37	346992.0
38	360910.0
39	375738.0
40	390360.0
41	402717.0
42	408193.5
43	413548.0
44	418332.0
45	420947.5
46	422583.5
47	419073.5
48	412000.5
49	405802.0
50	397349.5
51	381376.0
52	362086.5
53	340570.5
54	316121.5
55	290001.5
56	263361.0
57	235940.0
58	208398.0
59	179383.5
60	148866.0
61	120248.0
62	94334.0
63	71960.0
64	53223.0
65	39190.5
66	29284.5
67	22145.5
68	16918.5
69	12200.5
70	8520.0
71	6021.0
72	4191.5
73	2808.5
74	1859.5
75	1210.0
76	752.0
77	449.0
78	311.0
79	226.0
80	163.5
81	125.5
82	88.5
83	53.0
84	34.5
85	24.0
86	16.0
87	14.0
88	11.5
89	9.5
90	8.5
91	6.5
92	4.5
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.00656814877782E-5
2	0.004700268205922659
3	0.004519874116995989
4	3.00656814877782E-5
5	3.00656814877782E-5
6	3.00656814877782E-5
7	3.00656814877782E-5
8	0.0
9	0.00300656814877782
10-11	0.0010973973743039042
12-13	0.0
14-15	6.113355235848235E-4
16-17	4.50985222316673E-5
18-19	3.00656814877782E-5
20-21	5.01106719244788E-6
22-23	6.214167058058963E-4
24-25	0.004200354714442043
26-27	0.02119416775813037
28-29	0.3335242573791258
30-31	0.32648706278816414
32-33	0.33724737589530607
34-35	0.17907748442877458
36-37	0.03788962974927042
38-39	0.03437530995020144
40-41	0.011965999927926796
42-43	0.009463049017146011
44-45	0.00865046757916404
46-47	0.005751100893023145
48-49	0.00853391902742923
50-51	0.008716679713042392
52-53	0.0028480783565916473
54-55	0.0034506040031155897
56-57	0.0031514371903416825
58-59	0.00367041497600494
60-61	0.002346507795738854
62-63	0.0010142404458125043
64-65	4.405250807233605E-4
66-67	0.006898694891831586
68-69	0.016662831292165732
70-71	0.013358913880106585
72-73	0.014477668659163535
74-75	0.007162960368173747
76-77	0.004396974490708216
78-79	0.0013282643470928959
80-81	0.0014196478385298803
82-83	5.635611135486361E-4
84-85	7.052429427300416E-5
86-87	0.0
88-89	5.007285930455519E-4
90-91	0.0045494943504646775
92-93	0.017600994636493197
94-95	0.001482852850898938
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	137.0
20-21	546.0
22-23	1340.0
24-25	3141.0
26-27	4812.0
28-29	8218.0
30-31	19999.0
32-33	26230.0
34-35	38277.0
36-37	54482.0
38-39	64324.0
40-41	68429.0
42-43	81283.0
44-45	97109.0
46-47	96078.0
48-49	84756.0
50-51	76067.0
52-53	75204.0
54-55	81210.0
56-57	84730.0
58-59	83895.0
60-61	83039.0
62-63	83197.0
64-65	90703.0
66-67	95510.0
68-69	93580.0
70-71	91772.0
72-73	89009.0
74-75	90766.0
76-77	92985.0
78-79	97378.0
80-81	99463.0
82-83	97278.0
84-85	99653.0
86-87	106630.0
88-89	113763.0
90-91	122925.0
92-93	128676.0
94-95	149132.0
96-97	227676.0
98-99	891308.0
100-101	5983444.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.64489482026633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.02825852650552	77.1046073235431
2	6.954574729245526	11.912476424118305
3	1.7188028930262425	4.41620078970006
4	0.593789261477946	2.0342007537872924
5	0.2842051898386244	1.2170361795551408
6	0.14773160179730138	0.7591474498535606
7	0.07980779766007765	0.47845913055041117
8	0.05473962288889596	0.37505353958564297
9	0.03420299289202411	0.26363805558981496
>10	0.10214507661332724	1.2833729921117225
>50	0.0013664985625958248	0.071489066276751
>100	3.3908663781978364E-4	0.058292862336815465
>500	1.8362498567967667E-5	0.008932676188228208
>1k	1.836035563301854E-5	0.0170927568030613
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.00218938292594E-5	0.0
2	0.0	2.00437876585188E-5	0.0	1.00218938292594E-5	0.0
3	0.0	2.00437876585188E-5	0.0	2.00437876585188E-5	0.0
4	0.0	3.00656814877782E-5	0.0	2.00437876585188E-5	1.00218938292594E-5
5	0.0	3.00656814877782E-5	0.0	2.00437876585188E-5	1.00218938292594E-5
6	1.00218938292594E-5	3.00656814877782E-5	0.0	2.00437876585188E-5	1.00218938292594E-5
7	1.00218938292594E-5	4.00875753170376E-5	0.0	2.00437876585188E-5	1.00218938292594E-5
8	1.00218938292594E-5	4.00875753170376E-5	0.0	3.00656814877782E-5	2.00437876585188E-5
9	1.00218938292594E-5	1.0021893829259401E-4	0.0	7.01532568048158E-5	3.00656814877782E-5
10-11	2.00437876585188E-5	1.0021893829259401E-4	0.0	1.202627259511128E-4	3.00656814877782E-5
12-13	3.00656814877782E-5	1.0021893829259401E-4	0.0	1.653612481827801E-4	3.5076628402407896E-5
14-15	3.5076628402407896E-5	1.803940889266692E-4	0.0	2.104597704144474E-4	6.51423098901861E-5
16-17	4.00875753170376E-5	2.0043787658518801E-4	0.0	2.104597704144474E-4	8.01751506340752E-5
18-19	4.00875753170376E-5	2.104597704144474E-4	0.0	2.254926111583365E-4	8.01751506340752E-5
20-21	4.00875753170376E-5	2.204816642437068E-4	0.0	2.405254519022256E-4	8.01751506340752E-5
22-23	4.00875753170376E-5	2.3551450498759588E-4	0.0	2.7059113339000383E-4	9.01970444633346E-5
24-25	5.0109469146297004E-5	2.7059113339000383E-4	0.0	3.00656814877782E-4	1.102408321218534E-4
26-27	5.0109469146297004E-5	2.806130272192632E-4	0.0	3.407443901948196E-4	1.202627259511128E-4
28-29	5.0109469146297004E-5	2.806130272192632E-4	0.0	6.51423098901861E-4	1.202627259511128E-4
30-31	5.0109469146297004E-5	3.207006025363008E-4	0.0	0.0013980541891816863	1.252736728657425E-4
32-33	5.0109469146297004E-5	3.307224963655602E-4	0.0	0.0031418637154728216	1.403065136096316E-4
34-35	5.5120416060926706E-5	3.808319655118572E-4	0.0	0.005692435695019339	1.50328407438891E-4
36-37	6.01313629755564E-5	4.3595238157278387E-4	0.0	0.008934518348784755	1.50328407438891E-4
38-39	6.01313629755564E-5	4.810509038044512E-4	0.0	0.014160935980743533	1.603503012681504E-4
40-41	7.01532568048158E-5	5.111165852922294E-4	0.0	0.02006884239309195	1.803940889266692E-4
42-43	7.01532568048158E-5	5.361713198653779E-4	0.0	0.025630993468330917	1.9542692967055832E-4
44-45	7.01532568048158E-5	6.163464704994531E-4	0.0	0.031989885102996	2.054488234998177E-4
46-47	7.01532568048158E-5	6.714668865603798E-4	0.0	0.03919562676623352	2.204816642437068E-4
48-49	7.01532568048158E-5	6.814887803896393E-4	0.0	0.04669200335051955	2.405254519022256E-4
50-51	8.01751506340752E-5	7.416201433651956E-4	0.0	0.05372737281865964	2.405254519022256E-4
52-53	8.01751506340752E-5	7.616639310237144E-4	0.0	0.060762742286799745	2.605692395607444E-4
54-55	8.01751506340752E-5	7.616639310237144E-4	0.0	0.06796347300312262	2.906349210485226E-4
56-57	8.01751506340752E-5	7.616639310237144E-4	0.0	0.07569035314548161	3.00656814877782E-4
58-59	8.01751506340752E-5	7.766967717676035E-4	0.0	0.08234990159502449	3.106787087070414E-4
60-61	8.01751506340752E-5	8.318171878285302E-4	0.0	0.08833297221109235	3.207006025363008E-4
62-63	8.01751506340752E-5	8.618828693163084E-4	0.0	0.0939752984369654	3.307224963655602E-4
64-65	8.01751506340752E-5	9.420580199503836E-4	0.0	0.09981806253942363	3.357334432801899E-4
66-67	8.01751506340752E-5	9.470689668650133E-4	0.0	0.10591638493452797	3.457553371094493E-4
68-69	8.01751506340752E-5	9.570908606942726E-4	0.0	0.11242560497663195	3.50766284024079E-4
70-71	8.01751506340752E-5	9.671127545235321E-4	0.0	0.11885464986810185	3.50766284024079E-4
72-73	8.51860975487049E-5	9.771346483527915E-4	0.0	0.1235849837555123	3.708100716825978E-4
74-75	9.01970444633346E-5	0.0010072003298405695	0.0	0.1277190149600818	3.708100716825978E-4
76-77	9.01970444633346E-5	0.0010322550644137182	0.0	0.13135696242010297	3.758210185972275E-4
78-79	9.01970444633346E-5	0.0010573097989868669	0.0	0.13474937348130728	3.808319655118572E-4
80-81	9.01970444633346E-5	0.0010773535866453856	0.0	0.13616747145814748	3.808319655118572E-4
82-83	9.01970444633346E-5	0.001087375480474645	0.0	0.13682390550396395	3.8584291242648686E-4
84-85	9.01970444633346E-5	0.0010923864273892747	0.0	0.1372548469386221	3.908538593411166E-4
86-87	9.52079913779643E-5	0.001102408321218534	0.0	0.13741519723989026	3.908538593411166E-4
88-89	1.102408321218534E-4	0.0011775725249379794	0.0	0.13745027386829267	3.958648062557463E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11700	0.0	50.753067	1
GTATCAA	22120	0.0	37.506264	1
TCAACGC	26970	0.0	30.021778	4
ATCAACG	27215	0.0	29.81445	3
CAACGCA	27625	0.0	29.325302	5
TATCAAC	28540	0.0	28.62348	2
AACGCAG	28375	0.0	28.550184	6
ACGCAGA	32855	0.0	24.644264	7
CGCAGAG	33670	0.0	24.098125	8
GTACATG	27790	0.0	20.69623	1
GCAGAGT	39135	0.0	20.473146	9
TACATGG	27745	0.0	20.01129	2
ACATGGG	27885	0.0	19.51534	3
GAGTACT	23635	0.0	19.066053	12-13
AGAGTAC	35665	0.0	18.771435	10-11
CAGAGTA	38220	0.0	17.999287	10-11
GTGGTAT	6120	0.0	17.742249	1
CATGGGG	19610	0.0	17.454498	4
TGGTATC	5845	0.0	17.270805	2
AGTACTT	25860	0.0	17.228798	12-13
>>END_MODULE