FastQCFastQC Report
Fri 27 May 2016
SRR2139645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8996126
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114650.049.7768141
GTATCAA204450.036.7752341
TCAACGC258150.028.318034
ATCAACG259050.028.2204323
CAACGCA263750.027.7167765
TATCAAC270500.027.2151742
AACGCAG270800.027.1048726
ACGCAGA313100.023.3752337
CGCAGAG320100.022.8773168
GTGGTAT56050.021.4234641
TGGTATC55200.020.369752
GCAGAGT365450.019.87649
GTACATG256050.019.3054541
TACATGG254500.018.856182
AGAGTAC336350.018.82658810-11
ACATGGG255350.018.3772813
GAGTACT222650.018.19808812-13
CAGAGTA361450.017.70703310-11
AGTACTT242450.016.92191112-13
CATGGGG176850.016.6016394
Produced by FastQC (version 0.11.5)