FastQCFastQC Report
Fri 27 May 2016
SRR2139644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8300298
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105300.051.988221
GTATCAA190750.037.8694041
TCAACGC245500.028.883594
ATCAACG246800.028.731623
CAACGCA251600.028.1663135
TATCAAC253950.028.124942
AACGCAG260200.027.301126
ACGCAGA302450.023.4732177
CGCAGAG309650.022.8997948
TGGTATC49250.021.4493472
GTGGTAT51200.021.3842181
GTACATG251950.021.2187121
TACATGG254850.020.5073682
GCAGAGT351150.020.0594529
ACATGGG256750.019.7724743
GAGTACT210850.019.136412-13
AGAGTAC325650.018.45778710-11
CAGAGTA347550.017.79306810-11
CATGGGG185850.017.6962764
GTACTTT232450.017.3034114-15
Produced by FastQC (version 0.11.5)