FastQCFastQC Report
Fri 27 May 2016
SRR2139643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9100874
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116350.051.4541171
GTATCAA212550.037.35451
TCAACGC265500.029.21434
ATCAACG266400.029.196263
CAACGCA272700.028.489775
TATCAAC278450.028.20832
AACGCAG281550.027.6546966
ACGCAGA325700.023.8667967
CGCAGAG334750.023.2469758
GTACATG263700.020.8631941
TACATGG264850.020.3870352
GCAGAGT383200.020.07499
ACATGGG269700.019.6730063
GTGGTAT56300.019.4986131
GAGTACT232600.019.16034512-13
AGAGTAC348450.018.87083610-11
TGGTATC54200.018.4484982
CAGAGTA375100.018.02351410-11
AGTACTT255300.017.2233912-13
GTACTTT259150.017.16528114-15
Produced by FastQC (version 0.11.5)