##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139643_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9100874
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58554683868824	34.0	31.0	34.0	31.0	34.0
2	32.71728572442603	34.0	31.0	34.0	31.0	34.0
3	32.787236698365454	34.0	31.0	34.0	31.0	34.0
4	36.27223616105442	37.0	37.0	37.0	35.0	37.0
5	36.20168513485628	37.0	37.0	37.0	35.0	37.0
6	36.23028041043091	37.0	37.0	37.0	35.0	37.0
7	36.17827331748577	37.0	37.0	37.0	35.0	37.0
8	36.155343212091495	37.0	37.0	37.0	35.0	37.0
9	37.87444887161387	39.0	38.0	39.0	35.0	39.0
10-11	37.81026404716734	39.0	38.0	39.0	35.0	39.0
12-13	37.77765910175221	39.0	38.0	39.0	35.0	39.0
14-15	39.1492426991078	40.0	39.0	41.0	36.0	41.0
16-17	39.111439956206404	40.0	38.0	41.0	36.0	41.0
18-19	38.996550770838056	40.0	38.5	41.0	35.5	41.0
20-21	39.057186635531394	40.0	39.0	41.0	36.0	41.0
22-23	38.968845560575375	40.0	38.0	41.0	35.0	41.0
24-25	38.8574780020344	40.0	38.0	41.0	35.0	41.0
26-27	38.8046261859829	40.0	38.0	41.0	35.0	41.0
28-29	38.69735522016309	40.0	38.0	41.0	35.0	41.0
30-31	38.629873103661026	40.0	38.0	41.0	35.0	41.0
32-33	38.496203512366904	40.0	38.0	41.0	34.0	41.0
34-35	38.26281171563397	40.0	38.0	41.0	34.0	41.0
36-37	38.525563970737835	40.0	38.0	41.0	34.0	41.0
38-39	38.617280761408956	40.0	38.0	41.0	35.0	41.0
40-41	38.61019720218712	40.0	38.0	41.0	34.5	41.0
42-43	38.538485206674025	40.0	38.0	41.0	34.0	41.0
44-45	38.46244474328706	40.0	38.0	41.0	34.0	41.0
46-47	38.38627939742958	40.0	38.0	41.0	34.0	41.0
48-49	38.314243415055444	40.0	38.0	41.0	34.0	41.0
50-51	38.22918754230277	40.0	38.0	41.0	33.0	41.0
52-53	38.12641338667103	40.0	37.0	41.0	33.0	41.0
54-55	38.000646501749344	40.0	37.0	41.0	33.0	41.0
56-57	37.84911013144901	40.0	37.0	41.0	33.0	41.0
58-59	37.6765322420854	39.5	36.0	41.0	33.0	41.0
60-61	37.503060899703655	39.0	36.0	41.0	33.0	41.0
62-63	37.27995839381287	39.0	35.5	41.0	32.0	41.0
64-65	36.992209109344756	38.5	35.0	40.0	32.0	41.0
66-67	36.66644312320059	38.0	35.0	40.0	31.0	41.0
68-69	36.34283522625587	37.0	35.0	40.0	31.0	41.0
70-71	35.99957022204241	37.0	35.0	39.0	31.0	41.0
72-73	35.678772215279565	36.0	34.0	39.0	31.0	40.5
74-75	35.35091686457784	36.0	34.0	39.0	30.0	40.0
76-77	34.43491132976114	35.0	33.5	37.0	29.5	39.0
78-79	34.68972385707988	35.0	34.0	37.0	30.0	39.0
80-81	34.49574020285034	35.0	34.0	37.0	30.0	39.0
82-83	34.24923504687847	35.0	34.0	36.0	30.0	38.5
84-85	33.995223720166024	35.0	34.0	36.0	30.0	37.0
86-87	33.789778021869765	35.0	34.0	35.5	30.0	37.0
88-89	33.588940527737954	35.0	33.0	35.0	29.0	36.5
90-91	33.40833432233197	35.0	33.0	35.0	29.0	36.0
92-93	33.27481317011152	35.0	33.0	35.0	29.0	36.0
94-95	33.22075726932975	35.0	33.0	35.0	29.0	36.0
96-97	33.21638244858241	35.0	33.0	35.0	29.0	35.0
98-99	33.30474413610112	34.5	33.0	35.0	29.5	35.0
100-101	31.68932544117628	33.0	30.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	0.0
18	1.0
19	10.0
20	39.0
21	262.0
22	1218.0
23	3966.0
24	10350.0
25	21410.0
26	38034.0
27	59680.0
28	85862.0
29	115926.0
30	150008.0
31	188025.0
32	239076.0
33	309535.0
34	429737.0
35	620555.0
36	1039686.0
37	1997938.0
38	2530498.0
39	1195396.0
40	63661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.78085528507598	21.358502789622797	15.491141947716658	24.369499977584567
2	13.692687214988187	21.658260535135433	41.41123015218944	23.23782209768694
3	16.86918566670729	26.540067247127975	31.323856819153047	25.26689026701169
4	11.94912970976847	18.668068290598967	38.81134686873961	30.57145513089295
5	12.912312138880758	38.02408164843984	35.810590457705594	13.25301575497381
6	29.82668034447688	38.4278012041044	18.542486917737115	13.203031533681608
7	26.230266726089546	32.97678508169327	23.168164545111722	17.624783647105463
8	24.32820188478601	37.103832005585396	20.933703729993404	17.63426237963519
9	25.32870417986362	16.911409201871	21.37707865780921	36.38280796045616
10-11	23.929812921776477	27.030812263372244	28.99864649142624	20.04072832342504
12-13	24.7781174399423	24.59394280087196	29.139528702356355	21.488411056829385
14-15	21.88570457543314	26.01764425267398	26.817875177161422	25.278775994731458
16-17	21.414562952377892	29.159846575065508	27.507696381691087	21.917894090865513
18-19	21.51626949030911	28.19091781235396	29.3829413432937	20.909871354043226
20-21	22.203345011467068	27.321415728906135	29.338205452119148	21.13703380750765
22-23	21.941200239431176	27.282873736758233	29.165646966928545	21.61027905688205
24-25	21.74407615027437	27.420201275996988	29.246890946493124	21.58883162723552
26-27	21.703835998670012	27.577617033345312	29.17897446987576	21.539572498108914
28-29	21.64545634963998	27.55882562660173	29.22096457051426	21.574753453244032
30-31	21.880494674119728	27.537797059768902	29.07009327154934	21.511614994562024
32-33	21.75188039740624	27.624725705300946	28.99267353328781	21.630720364005004
34-35	21.859937498284708	27.614419915830872	28.981063471703322	21.544579114181094
36-37	21.81056535348694	27.59040681475157	28.95690647924795	21.642121352513534
38-39	21.658773583571175	27.646330760503023	29.05749134871388	21.63740430721192
40-41	21.924785683561794	27.55749411925797	28.877410873581177	21.640309323599062
42-43	21.8486537576476	27.68062199140854	28.62463678229734	21.84608746864652
44-45	21.906200949248557	27.77383722594649	28.447977296935857	21.8719845278691
46-47	22.004456576884724	27.75228768772959	28.17415103456909	22.069104700816595
48-49	21.948559682890934	27.888329866334328	28.082668654434777	22.080441796339965
50-51	21.985963963038614	27.996366712002462	27.977821853482222	22.039847471476705
52-53	22.13724308045175	27.909042968872587	27.912175285629985	22.041538665045678
54-55	22.111800211822484	27.82967648734639	27.890998547032396	22.167524753798734
56-57	22.10750437581487	27.967795446865708	27.83018859408536	22.094511583234063
58-59	22.018633280198188	28.02279343549252	27.897875860936878	22.060697423372414
60-61	22.15643062570511	28.071703341086003	27.770337709555058	22.001528323653826
62-63	22.116872548593207	28.117098414097963	27.763460743883346	22.002568293425483
64-65	22.076978939724036	28.041992231036954	27.764555987594154	22.116472841644853
66-67	22.146676617722726	28.056327697481176	27.62516636186938	22.17182932292672
68-69	22.08710893158657	27.983526074283287	27.749845829096962	22.179519165033177
70-71	22.071993586162783	27.820359816782425	27.799687862494842	22.307958734559953
72-73	22.156179182745326	27.8704225688897	27.67348253706117	22.299915711303807
74-75	22.023428479574758	27.99058822849879	27.686185104955314	22.299798186971138
76-77	22.13952203753492	27.88887330899775	27.683154628149637	22.28845002531769
78-79	22.08224125583107	27.860747990475698	27.668517317422936	22.38849343627029
80-81	22.11925902586124	27.906717872031134	27.64555151982998	22.328471582277643
82-83	22.197631300536173	27.848883634690903	27.706826734234234	22.246658330538686
84-85	22.18119021633926	27.835170339045856	27.688533087808082	22.2951063568068
86-87	22.132379278349987	27.813541242714706	27.743177085520127	22.31090239341518
88-89	22.128724313304502	27.790409140664448	27.73582796492489	22.34503858110616
90-91	22.191527505707683	27.79599779102238	27.74086018144181	22.271614521828127
92-93	22.199346831636227	27.687670428442967	27.823696910579248	22.28928582934156
94-95	22.034625536379874	27.680476876941178	27.902751423327487	22.382146163351464
96-97	22.040208243426886	27.735096740806853	27.909469654559487	22.315225361206775
98-99	22.136310419187126	28.303919345772456	27.964126546416455	21.595643688623966
100-101	20.513787288091304	31.918855773212158	25.905689300831842	21.661667637864703
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2574.0
1	2183.5
2	2518.0
3	3410.5
4	4470.0
5	6545.5
6	8356.5
7	10597.5
8	12637.0
9	13137.0
10	12367.5
11	11541.5
12	12864.5
13	17234.5
14	27898.0
15	42540.5
16	53969.5
17	59648.5
18	59857.0
19	56213.0
20	50852.0
21	45969.0
22	43740.5
23	46005.5
24	53251.5
25	65444.5
26	80939.0
27	97823.5
28	116835.0
29	138031.5
30	158975.0
31	179501.5
32	201506.0
33	224637.0
34	246240.0
35	265418.0
36	286077.5
37	303999.0
38	317367.5
39	330539.5
40	343066.0
41	353182.5
42	357421.0
43	361331.5
44	366080.0
45	369788.0
46	373776.5
47	372904.0
48	368526.0
49	363776.0
50	357995.0
51	348441.5
52	332867.5
53	313580.5
54	293321.5
55	273343.5
56	251225.5
57	226357.0
58	201473.0
59	174537.5
60	145309.0
61	117839.5
62	93763.5
63	72441.0
64	53791.0
65	39884.0
66	30154.0
67	22832.5
68	17390.5
69	12846.5
70	9219.5
71	6617.0
72	4566.0
73	3026.5
74	1954.5
75	1270.0
76	838.5
77	539.0
78	348.5
79	234.5
80	165.0
81	119.5
82	90.5
83	57.5
84	32.5
85	26.5
86	23.0
87	18.0
88	13.0
89	8.5
90	6.0
91	5.5
92	5.5
93	4.0
94	3.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.1975911324560695E-5
2	0.00410949541769285
3	0.004516049777197223
4	2.1975911324560695E-5
5	2.1975911324560695E-5
6	2.1975911324560695E-5
7	2.1975911324560695E-5
8	0.0
9	0.003186507142061301
10-11	9.010123643069885E-4
12-13	1.0987955662280348E-5
14-15	6.977351845548021E-4
16-17	7.142171180482227E-5
18-19	5.493977831140174E-5
20-21	1.0988253282534637E-5
22-23	7.197828695303439E-4
24-25	0.004050218755777195
26-27	0.02065925905605877
28-29	0.3321695554292131
30-31	0.3247728910866783
32-33	0.3354964158844386
34-35	0.17884597716925807
36-37	0.040303118128650416
38-39	0.03727330853289933
40-41	0.014084568836136504
42-43	0.010994744557417349
44-45	0.009158967915158361
46-47	0.005863117556949713
48-49	0.00914712284824859
50-51	0.00972981548015993
52-53	0.003238793522768215
54-55	0.0038011548469348935
56-57	0.00392957858188309
58-59	0.005044528741156975
60-61	0.0035150943294547272
62-63	0.0022178314513581687
64-65	6.297535481312531E-4
66-67	0.00651294848484505
68-69	0.015518130848140958
70-71	0.012707713395531246
72-73	0.013487490936873864
74-75	0.006799495288428881
76-77	0.004423933253393015
78-79	0.0015958062747505027
80-81	0.0011328091931468165
82-83	6.108608450072387E-4
84-85	7.640245032382137E-5
86-87	0.0
88-89	3.56458863506483E-4
90-91	0.004652471587544139
92-93	0.01702431579716046
94-95	0.0015647092627943432
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	149.0
20-21	537.0
22-23	1197.0
24-25	2901.0
26-27	4573.0
28-29	7660.0
30-31	18012.0
32-33	23601.0
34-35	34388.0
36-37	47619.0
38-39	56558.0
40-41	60095.0
42-43	71682.0
44-45	85808.0
46-47	84831.0
48-49	74443.0
50-51	66467.0
52-53	64273.0
54-55	69254.0
56-57	72212.0
58-59	71888.0
60-61	71416.0
62-63	72968.0
64-65	78928.0
66-67	83971.0
68-69	80663.0
70-71	79286.0
72-73	77724.0
74-75	79545.0
76-77	79959.0
78-79	85309.0
80-81	86859.0
82-83	85745.0
84-85	87950.0
86-87	94259.0
88-89	101154.0
90-91	109892.0
92-93	116269.0
94-95	135040.0
96-97	210644.0
98-99	827907.0
100-101	5537238.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.19844525817737
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.27837869602956	77.81855884026709
2	6.917838304463627	11.92613812784461
3	1.6129631298966318	4.171047421675597
4	0.5667202917239291	1.9540163217145363
5	0.2529704725304085	1.090283071417384
6	0.13558740634335784	0.7012454174031708
7	0.07039325615532817	0.4247452466074938
8	0.04130905031544523	0.284862072982656
9	0.02825229539694856	0.21917735443725542
>10	0.09321098184806362	1.2235079075727706
>50	0.0019898001910148666	0.11037477633727251
>100	3.468374926470305E-4	0.051967876271866775
>500	3.9477613035870705E-5	0.02407556546828366
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0987955662280348E-5
2	0.0	0.0	0.0	2.1975911324560695E-5	1.0987955662280348E-5
3	0.0	1.0987955662280348E-5	0.0	2.1975911324560695E-5	1.0987955662280348E-5
4	0.0	1.0987955662280348E-5	0.0	2.1975911324560695E-5	1.0987955662280348E-5
5	0.0	4.395182264912139E-5	0.0	3.2963866986841046E-5	2.1975911324560695E-5
6	4.395182264912139E-5	4.395182264912139E-5	0.0	3.2963866986841046E-5	3.2963866986841046E-5
7	4.395182264912139E-5	4.395182264912139E-5	0.0	4.395182264912139E-5	3.2963866986841046E-5
8	4.395182264912139E-5	4.395182264912139E-5	0.0	5.493977831140174E-5	4.395182264912139E-5
9	7.691568963596244E-5	5.493977831140174E-5	0.0	8.790364529824278E-5	4.395182264912139E-5
10-11	7.691568963596244E-5	5.493977831140174E-5	5.493977831140174E-6	1.3185546794736418E-4	4.395182264912139E-5
12-13	7.691568963596244E-5	6.043375614254191E-5	1.0987955662280348E-5	1.5383137927192487E-4	4.395182264912139E-5
14-15	7.691568963596244E-5	8.240966746710262E-5	1.0987955662280348E-5	1.8130126842762575E-4	5.493977831140174E-5
16-17	1.8679524625876592E-4	9.889160096052314E-5	1.0987955662280348E-5	2.0327717975218644E-4	5.493977831140174E-5
18-19	1.9228922408990608E-4	1.0987955662280348E-4	1.6481933493420523E-5	2.2525309107674713E-4	7.142171180482226E-5
20-21	2.087711575833266E-4	1.3185546794736418E-4	2.1975911324560695E-5	2.4722900240130785E-4	8.790364529824278E-5
22-23	2.3624104673902746E-4	1.3185546794736418E-4	2.1975911324560695E-5	2.6371093589472837E-4	9.889160096052314E-5
24-25	2.582169580635882E-4	1.4284342360964451E-4	2.1975911324560695E-5	3.7359049251753184E-4	1.0987955662280348E-4
26-27	2.6371093589472837E-4	1.5383137927192487E-4	2.1975911324560695E-5	4.7797607130919517E-4	1.2086751228508383E-4
28-29	2.6371093589472837E-4	1.5932535710306504E-4	2.1975911324560695E-5	0.0011152774997214554	1.26361490116224E-4
30-31	2.911808250504292E-4	1.8679524625876592E-4	2.1975911324560695E-5	0.0025382177579867603	1.3185546794736418E-4
32-33	3.0766275854384974E-4	1.8679524625876592E-4	2.1975911324560695E-5	0.005345640429699389	1.3185546794736418E-4
34-35	3.186507142061301E-4	1.9228922408990608E-4	2.1975911324560695E-5	0.009565015404015043	1.4284342360964451E-4
36-37	3.2963866986841046E-4	1.9778320192104628E-4	3.2963866986841046E-5	0.014921643789376713	1.4284342360964451E-4
38-39	3.406266255306908E-4	2.087711575833266E-4	3.2963866986841046E-5	0.021591332876380882	1.5383137927192487E-4
40-41	3.626025368552515E-4	2.307470689078873E-4	3.2963866986841046E-5	0.030255335916088935	1.5383137927192487E-4
42-43	3.6809651468639164E-4	2.3624104673902746E-4	3.2963866986841046E-5	0.03760078427632335	1.7580729059648556E-4
44-45	3.7908447034867203E-4	2.4722900240130785E-4	3.2963866986841046E-5	0.0465504741632507	1.9778320192104628E-4
46-47	3.8457844817981217E-4	2.801928693881489E-4	3.2963866986841046E-5	0.05543423631620435	2.142651354144668E-4
48-49	3.8457844817981217E-4	3.0766275854384974E-4	3.2963866986841046E-5	0.06276320274294535	2.307470689078873E-4
50-51	4.285302708289336E-4	3.516145811929711E-4	3.2963866986841046E-5	0.06976253049981793	2.307470689078873E-4
52-53	4.450122043223541E-4	3.7359049251753184E-4	3.2963866986841046E-5	0.07699809930342955	2.4722900240130785E-4
54-55	4.5050618215349426E-4	3.7908447034867203E-4	3.2963866986841046E-5	0.0845688007547407	2.6371093589472837E-4
56-57	4.5050618215349426E-4	3.9556640384209255E-4	3.845784481798122E-5	0.09131540553138083	2.746988915570087E-4
58-59	4.5050618215349426E-4	4.010603816732327E-4	4.395182264912139E-5	0.09739174501262186	2.746988915570087E-4
60-61	4.669881156469148E-4	4.065543595043729E-4	4.395182264912139E-5	0.10327030129194184	2.8568684721928903E-4
62-63	5.219278939583166E-4	4.230362929977934E-4	4.395182264912139E-5	0.10915984552692412	2.911808250504292E-4
64-65	5.329158496205969E-4	4.944580048026157E-4	5.493977831140174E-5	0.1148461125821542	2.966748028815694E-4
66-67	5.38409827451737E-4	5.164339161271764E-4	5.493977831140174E-5	0.12101584968652462	2.966748028815694E-4
68-69	5.38409827451737E-4	5.38409827451737E-4	5.493977831140174E-5	0.1266197070742876	2.966748028815694E-4
70-71	5.38409827451737E-4	5.38409827451737E-4	5.493977831140174E-5	0.132240046395544	3.0216878071270955E-4
72-73	5.603857387762978E-4	5.493977831140174E-4	5.493977831140174E-5	0.13648689125901534	3.0766275854384974E-4
74-75	5.713736944385781E-4	5.548917609451576E-4	5.493977831140174E-5	0.14042607336394286	3.186507142061301E-4
76-77	5.713736944385781E-4	5.878556279319987E-4	5.493977831140174E-5	0.14394221917587258	3.351326476995506E-4
78-79	5.713736944385781E-4	5.933496057631388E-4	5.493977831140174E-5	0.14701335278347993	3.406266255306908E-4
80-81	5.713736944385781E-4	5.933496057631388E-4	5.493977831140174E-5	0.14846925690873208	3.46120603361831E-4
82-83	5.713736944385781E-4	6.043375614254191E-4	5.493977831140174E-5	0.14886482331257417	3.516145811929711E-4
84-85	5.713736944385781E-4	6.153255170876995E-4	5.493977831140174E-5	0.14917798004894914	3.571085590241113E-4
86-87	5.713736944385781E-4	6.263134727499799E-4	7.142171180482226E-5	0.1493208234725588	3.6809651468639164E-4
88-89	5.713736944385781E-4	6.3180745058112E-4	7.691568963596244E-5	0.14935928131737677	3.7359049251753184E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11635	0.0	51.454117	1
GTATCAA	21255	0.0	37.3545	1
TCAACGC	26550	0.0	29.2143	4
ATCAACG	26640	0.0	29.19626	3
CAACGCA	27270	0.0	28.48977	5
TATCAAC	27845	0.0	28.2083	2
AACGCAG	28155	0.0	27.654696	6
ACGCAGA	32570	0.0	23.866796	7
CGCAGAG	33475	0.0	23.246975	8
GTACATG	26370	0.0	20.863194	1
TACATGG	26485	0.0	20.387035	2
GCAGAGT	38320	0.0	20.0749	9
ACATGGG	26970	0.0	19.673006	3
GTGGTAT	5630	0.0	19.498613	1
GAGTACT	23260	0.0	19.160345	12-13
AGAGTAC	34845	0.0	18.870836	10-11
TGGTATC	5420	0.0	18.448498	2
CAGAGTA	37510	0.0	18.023514	10-11
AGTACTT	25530	0.0	17.22339	12-13
GTACTTT	25915	0.0	17.165281	14-15
>>END_MODULE