FastQCFastQC Report
Fri 27 May 2016
SRR2139642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8718583
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108150.052.114881
GTATCAA201300.037.0446781
TCAACGC250700.029.2204594
ATCAACG252550.029.0412773
CAACGCA258100.028.3826775
TATCAAC260400.028.2305262
AACGCAG264800.027.6965186
ACGCAGA306700.023.9403537
CGCAGAG313000.023.4584888
GCAGAGT363200.020.0179569
GTACATG250950.019.675311
TACATGG253500.019.2101212
GAGTACT215850.019.02775612-13
TGGTATC52900.018.571312
ACATGGG253950.018.5259743
AGAGTAC329650.018.3869610-11
GTGGTAT57200.018.2856831
CAGAGTA358300.017.86211810-11
GTACTTT240750.016.97237414-15
AGTACTT237350.016.75113512-13
Produced by FastQC (version 0.11.5)