##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139642_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8718583
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.555920956421474	34.0	31.0	34.0	31.0	34.0
2	32.684992389244904	34.0	31.0	34.0	31.0	34.0
3	32.75542160922251	34.0	31.0	34.0	31.0	34.0
4	36.24987982565516	37.0	35.0	37.0	35.0	37.0
5	36.179203432484385	37.0	35.0	37.0	35.0	37.0
6	36.20854730636848	37.0	37.0	37.0	35.0	37.0
7	36.15471619642779	37.0	36.0	37.0	35.0	37.0
8	36.13262373025525	37.0	36.0	37.0	35.0	37.0
9	37.843987262609076	39.0	38.0	39.0	35.0	39.0
10-11	37.77838508849431	39.0	38.0	39.0	35.0	39.0
12-13	37.74705797949048	39.0	38.0	39.0	35.0	39.0
14-15	39.10794535075252	40.0	38.5	41.0	36.0	41.0
16-17	39.071260777123996	40.0	38.0	41.0	36.0	41.0
18-19	38.95918620032636	40.0	38.5	41.0	35.0	41.0
20-21	39.022241750343454	40.0	38.5	41.0	35.0	41.0
22-23	38.9365338342957	40.0	38.0	41.0	35.0	41.0
24-25	38.82413876587128	40.0	38.0	41.0	35.0	41.0
26-27	38.772773919988595	40.0	38.0	41.0	34.5	41.0
28-29	38.661967691648904	40.0	38.0	41.0	35.0	41.0
30-31	38.596248345267725	40.0	38.0	41.0	34.0	41.0
32-33	38.461828357188026	40.0	38.0	41.0	34.0	41.0
34-35	38.229221949857475	40.0	38.0	41.0	34.0	41.0
36-37	38.4953576905708	40.0	38.0	41.0	34.0	41.0
38-39	38.59050119448926	40.0	38.0	41.0	34.0	41.0
40-41	38.58796349159293	40.0	38.0	41.0	34.0	41.0
42-43	38.51707556464863	40.0	38.0	41.0	34.0	41.0
44-45	38.44215463299247	40.0	38.0	41.0	34.0	41.0
46-47	38.366371293104734	40.0	38.0	41.0	34.0	41.0
48-49	38.29545172406783	40.0	38.0	41.0	33.0	41.0
50-51	38.21677804430226	40.0	38.0	41.0	33.0	41.0
52-53	38.11993667398737	40.0	37.0	41.0	33.0	41.0
54-55	37.996861885395134	40.0	37.0	41.0	33.0	41.0
56-57	37.846272853120794	40.0	37.0	41.0	33.0	41.0
58-59	37.67428395811125	39.5	36.0	41.0	33.0	41.0
60-61	37.506993313468286	39.0	36.0	41.0	32.5	41.0
62-63	37.29055563850582	39.0	35.5	41.0	32.0	41.0
64-65	37.006036117303836	38.5	35.0	40.0	32.0	41.0
66-67	36.681016918701076	38.0	35.0	40.0	31.0	41.0
68-69	36.361387818583935	37.0	35.0	40.0	31.0	41.0
70-71	36.022769503886636	37.0	35.0	39.5	31.0	41.0
72-73	35.705529733265905	36.0	34.0	39.0	31.0	41.0
74-75	35.3786226009106	36.0	34.0	39.0	30.0	40.0
76-77	34.45807014085409	35.0	33.5	37.0	29.5	39.0
78-79	34.70936264685201	35.0	34.0	37.0	30.0	39.0
80-81	34.516807738986664	35.0	34.0	37.0	30.0	39.0
82-83	34.267918659161	35.0	34.0	36.0	30.0	38.5
84-85	34.01072833833119	35.0	34.0	36.0	30.0	37.0
86-87	33.8013575263374	35.0	34.0	35.5	30.0	37.0
88-89	33.59838513004675	35.0	33.0	35.0	29.0	36.5
90-91	33.415528193817124	35.0	33.0	35.0	29.0	36.0
92-93	33.279643486749336	35.0	33.0	35.0	29.0	36.0
94-95	33.22442518684501	35.0	33.0	35.0	29.0	36.0
96-97	33.2207755532763	35.0	33.0	35.0	29.0	35.0
98-99	33.3078603831552	34.5	33.0	35.0	29.5	35.0
100-101	31.717852801288636	33.0	30.0	34.5	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	1.0
19	9.0
20	47.0
21	265.0
22	1109.0
23	3684.0
24	9653.0
25	20283.0
26	36454.0
27	57864.0
28	83136.0
29	112348.0
30	145390.0
31	182738.0
32	232255.0
33	299016.0
34	413356.0
35	593926.0
36	986235.0
37	1880288.0
38	2410931.0
39	1185962.0
40	63632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.617678884020364	21.56395386313967	15.437361258975372	24.381005993864598
2	13.599141846717968	21.82926264151913	41.560399235810465	23.01119627595244
3	16.838140276026323	26.756742310356067	31.372593326523024	25.032524087094583
4	11.87694283313502	18.782354745301472	39.12413738839642	30.216565033167093
5	12.788008416965052	38.14082381745105	35.91891433721676	13.152253428367136
6	29.499544765421714	38.679110892115254	18.69885492847346	13.12248941398957
7	25.957348803125463	33.11345433082417	23.391243737657827	17.537953128392537
8	24.0791536881624	37.32779741845665	21.118466154419817	17.474582738961136
9	25.239494803605083	17.00036887705383	21.607856622444242	36.15227969689684
10-11	23.802600920423096	27.193646652479575	29.134768226219894	19.868984200877435
12-13	24.619746121588797	24.74702024400066	29.306711881965224	21.326521752445323
14-15	21.7701446058823	26.161856506691212	26.947780731259012	25.120218156167475
16-17	21.26945264516518	29.323634425872157	27.670726981635134	21.736185947327524
18-19	21.347415519499734	28.415200640471273	29.46244113146866	20.77494270856034
20-21	22.076516101444906	27.525530066017094	29.435731402325306	20.962222430212694
22-23	21.819775349518693	27.42889094525795	29.28743485472143	21.46389885050193
24-25	21.590700678327476	27.613716071507348	29.338617085765918	21.456966164399255
26-27	21.575657846592524	27.734013968361836	29.26525911664188	21.42506906840376
28-29	21.514909296415322	27.748041085430074	29.311261105172605	21.425788512981995
30-31	21.738092839212204	27.710495951450277	29.17554503988859	21.375866169448933
32-33	21.617249455409475	27.77914007300669	29.095498783876927	21.5081116877069
34-35	21.73029629619767	27.769321537577767	29.08616393857159	21.414218227652974
36-37	21.664983901664275	27.742732754620114	29.07574625808343	21.516537085632184
38-39	21.540575294732346	27.826572692544598	29.15218475346047	21.48066725926258
40-41	21.76878013032218	27.744403777528454	28.984585612528768	21.5022304796206
42-43	21.713317530566837	27.831471676915005	28.751573744989656	21.703637047528506
44-45	21.788082008139163	27.952455209585914	28.545407944893462	21.71405483738146
46-47	21.88651499324242	27.938531119594924	28.285085782262826	21.889868104899833
48-49	21.844336309056985	28.033153878500876	28.212258793357996	21.91025101908414
50-51	21.79438511473549	28.13014851368076	28.135167698668578	21.940298672915173
52-53	22.00464082128293	28.07643477731205	27.99974592276512	21.919178478639907
54-55	21.998471887845934	27.985166216323172	28.018384684514096	21.997977211316798
56-57	21.975305100324295	28.13479375231979	27.9355807973548	21.95432035000112
58-59	21.937498692462615	28.16602637730248	27.98476342630413	21.911711503930775
60-61	22.0269907625363	28.180947460701887	27.906641043100954	21.885420733660858
62-63	21.98089056191954	28.250713092812322	27.906263057012264	21.862133288255873
64-65	21.962983203821008	28.165538224247943	27.88781119444278	21.98366737748827
66-67	22.021805311002453	28.178507311608065	27.770910575539354	22.02877680185013
68-69	21.994631890781147	28.143575289526083	27.838023022568127	22.023769797124643
70-71	21.94417446297498	27.956970725629716	27.929254486748423	22.169600324646876
72-73	22.032810274464172	27.989754218751294	27.859481375192573	22.117954131591958
74-75	21.897085862983	28.08388850174023	27.83230114989388	22.186724485382893
76-77	22.025553933005778	28.028917269693576	27.830213495870087	22.115315301430556
78-79	22.00323367603742	27.98907046978867	27.74664397688863	22.261051877285283
80-81	21.98775389652171	28.022243025129473	27.77952623271872	22.210476845630097
82-83	22.06669677398925	27.9923073805598	27.84569033626122	22.095305509189732
84-85	22.08070583964731	27.924250816818745	27.81933737298384	22.175705970550105
86-87	21.986296414597795	27.936025694986334	27.900880463669687	22.176797426746184
88-89	22.036118824174718	27.90093366039897	27.884778353036687	22.178169162389626
90-91	22.06060489788871	27.944793715047627	27.87998733800804	22.114614049055625
92-93	22.097988095554943	27.866682266394914	27.903678286356843	22.1316513516933
94-95	21.915967017159165	27.8719520571031	27.96192289249797	22.250158033239764
96-97	21.894714179985645	27.866909719040684	28.06786615034975	22.170509950623924
98-99	22.06257385006418	28.40810289279733	28.0790397578993	21.450283499239184
100-101	20.422492225337525	32.0584173841185	26.006641749766285	21.51244864077769
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2432.0
1	2028.5
2	2317.0
3	3154.0
4	4101.5
5	6049.0
6	7841.5
7	9943.0
8	11748.0
9	12059.5
10	11153.0
11	10336.0
12	11656.5
13	15952.5
14	26198.0
15	40138.5
16	50905.5
17	56092.0
18	56180.5
19	52976.0
20	48709.5
21	44643.0
22	42880.5
23	45879.5
24	53818.5
25	66849.0
26	83499.0
27	100962.5
28	119883.5
29	140655.5
30	160799.0
31	180786.0
32	202745.5
33	225143.5
34	246195.5
35	265852.0
36	287104.5
37	304166.0
38	315434.0
39	327144.5
40	337609.5
41	345378.0
42	349195.5
43	352629.0
44	356240.0
45	359077.5
46	360801.0
47	357893.0
48	351938.5
49	346048.0
50	339210.5
51	327787.5
52	311932.0
53	293331.0
54	274336.0
55	255058.5
56	233644.5
57	210306.5
58	186010.0
59	160721.5
60	134327.0
61	109131.0
62	86133.0
63	66076.5
64	49003.0
65	35913.0
66	27044.0
67	20689.5
68	15674.0
69	11290.5
70	7934.5
71	5737.0
72	3989.0
73	2581.0
74	1739.0
75	1126.0
76	716.5
77	470.5
78	329.5
79	235.0
80	154.5
81	91.0
82	59.0
83	46.5
84	36.0
85	27.0
86	21.0
87	17.0
88	13.0
89	7.5
90	3.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.1469753743240157E-5
2	0.0037506094740395314
3	0.004920524355850027
4	1.1469753743240157E-5
5	1.1469753743240157E-5
6	1.1469753743240157E-5
7	0.0
8	0.0
9	0.002649513114688476
10-11	0.0010609522212497145
12-13	0.0
14-15	6.652457171079292E-4
16-17	7.455339933106102E-5
18-19	3.440926122972047E-5
20-21	5.735031124300663E-6
22-23	5.506052700495166E-4
24-25	0.0038262486162116607
26-27	0.020858940767494255
28-29	0.3344431102215952
30-31	0.3276421805708612
32-33	0.33815088110726554
34-35	0.1792900205334632
36-37	0.03865148739154119
38-39	0.035435703819184004
40-41	0.01292387350479515
42-43	0.009987554653871697
44-45	0.008560639125839183
46-47	0.005551961313401933
48-49	0.008897694093615459
50-51	0.009243067868442017
52-53	0.002880949183347145
54-55	0.003493922265864943
56-57	0.0033425359434768953
58-59	0.004101034436507345
60-61	0.00260109168590659
62-63	0.001397277790912911
64-65	3.5435958757268804E-4
66-67	0.006891417525451816
68-69	0.016591901395014073
70-71	0.013432467338118259
72-73	0.014462766156880436
74-75	0.00748221000651368
76-77	0.0046724448066581565
78-79	0.0014385229501455423
80-81	0.0012769496456428865
82-83	5.156663429589356E-4
84-85	7.354084303986664E-5
86-87	7.450758680953447E-6
88-89	5.213799794621625E-4
90-91	0.004481376924546121
92-93	0.017519306744254177
94-95	0.0015421516958978605
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	134.0
20-21	525.0
22-23	1150.0
24-25	2661.0
26-27	4241.0
28-29	7516.0
30-31	18016.0
32-33	24123.0
34-35	34598.0
36-37	49181.0
38-39	58030.0
40-41	60884.0
42-43	72714.0
44-45	86517.0
46-47	85532.0
48-49	75928.0
50-51	67564.0
52-53	66538.0
54-55	71065.0
56-57	73820.0
58-59	73396.0
60-61	73167.0
62-63	72676.0
64-65	79125.0
66-67	83672.0
68-69	81621.0
70-71	80299.0
72-73	77317.0
74-75	79438.0
76-77	79928.0
78-79	85851.0
80-81	86052.0
82-83	84743.0
84-85	87228.0
86-87	92110.0
88-89	99150.0
90-91	107489.0
92-93	113205.0
94-95	131053.0
96-97	199858.0
98-99	778514.0
100-101	5211954.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.47035491660371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.09291950930192	79.67929999868252
2	6.483249779556504	11.341843184616001
3	1.4349054160475816	3.7653505804031657
4	0.46310454043970195	1.6203167406300554
5	0.212949507595369	0.9313384504341462
6	0.11213174223413296	0.5884921974382038
7	0.0596639510190157	0.3653178879952113
8	0.04058111201913822	0.2839715416979578
9	0.020961243192829083	0.16501386434130033
>10	0.07752899848587418	1.078905086038326
>50	0.0016275509771131638	0.09289268419938752
>100	3.3763571767489724E-4	0.05780776474065909
>500	3.901341308588844E-5	0.02945001878305955
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1469753743240157E-5
2	0.0	1.1469753743240157E-5	0.0	1.1469753743240157E-5	1.1469753743240157E-5
3	0.0	1.1469753743240157E-5	0.0	2.2939507486480314E-5	1.1469753743240157E-5
4	0.0	5.734876871620079E-5	0.0	5.734876871620079E-5	2.2939507486480314E-5
5	0.0	5.734876871620079E-5	0.0	5.734876871620079E-5	2.2939507486480314E-5
6	0.0	5.734876871620079E-5	0.0	5.734876871620079E-5	3.4409261229720474E-5
7	0.0	5.734876871620079E-5	0.0	6.881852245944095E-5	3.4409261229720474E-5
8	0.0	6.881852245944095E-5	0.0	6.881852245944095E-5	3.4409261229720474E-5
9	0.0	1.1469753743240158E-4	0.0	1.1469753743240158E-4	3.4409261229720474E-5
10-11	1.1469753743240157E-5	1.1469753743240158E-4	0.0	1.7204630614860237E-4	3.4409261229720474E-5
12-13	2.2939507486480314E-5	1.2043241430402166E-4	0.0	1.835160598918425E-4	3.4409261229720474E-5
14-15	2.2939507486480314E-5	1.5484167553374213E-4	0.0	1.835160598918425E-4	4.014413810134055E-5
16-17	2.2939507486480314E-5	1.605765524053622E-4	0.0	1.9498581363508268E-4	4.587901497296063E-5
18-19	2.2939507486480314E-5	1.7204630614860237E-4	0.0	2.0645556737832282E-4	5.161389184458071E-5
20-21	2.2939507486480314E-5	1.835160598918425E-4	0.0	2.3512995173642322E-4	6.308364558782087E-5
22-23	3.4409261229720474E-5	2.2366019799318306E-4	0.0	2.5233458235128346E-4	8.602315307430119E-5
24-25	4.587901497296063E-5	2.580694592229035E-4	0.0	2.752740898377638E-4	1.0322778368916141E-4
26-27	4.587901497296063E-5	2.638043360945236E-4	0.0	4.301157653715059E-4	1.376370449188819E-4
28-29	4.587901497296063E-5	2.695392129661437E-4	0.0	0.0011297707437091555	1.4337192179050197E-4
30-31	4.587901497296063E-5	3.211531048107244E-4	0.0	0.002827294297708699	1.5484167553374213E-4
32-33	4.587901497296063E-5	3.211531048107244E-4	0.0	0.00583236977843762	1.7204630614860237E-4
34-35	5.161389184458071E-5	4.071762578850256E-4	0.0	0.010024564771591898	1.835160598918425E-4
36-37	5.734876871620079E-5	4.587901497296063E-4	0.0	0.015828260165671418	1.835160598918425E-4
38-39	5.734876871620079E-5	4.8746453408770663E-4	0.0	0.023725185617892268	1.835160598918425E-4
40-41	5.734876871620079E-5	5.390784259322874E-4	5.7348768716200785E-6	0.03349168093026126	1.835160598918425E-4
42-43	5.734876871620079E-5	5.734876871620079E-4	1.1469753743240157E-5	0.0423004518050697	1.892509367634626E-4
44-45	5.734876871620079E-5	6.365713327498287E-4	1.1469753743240157E-5	0.05257161628214126	1.9498581363508268E-4
46-47	5.734876871620079E-5	6.652457171079291E-4	1.1469753743240157E-5	0.06294027366603036	2.17925321121563E-4
48-49	5.734876871620079E-5	6.652457171079291E-4	1.1469753743240157E-5	0.07133039852921054	2.5233458235128346E-4
50-51	5.734876871620079E-5	7.3406423956737E-4	1.1469753743240157E-5	0.0795484770862421	2.5233458235128346E-4
52-53	5.734876871620079E-5	7.455339933106102E-4	1.1469753743240157E-5	0.08704969603432117	2.580694592229035E-4
54-55	5.734876871620079E-5	7.570037470538504E-4	1.1469753743240157E-5	0.09480324956475152	2.638043360945236E-4
56-57	5.734876871620079E-5	7.684735007970905E-4	1.1469753743240157E-5	0.10187435274745907	2.695392129661437E-4
58-59	6.881852245944095E-5	7.799432545403307E-4	1.1469753743240157E-5	0.10868738647094373	2.752740898377638E-4
60-61	6.881852245944095E-5	7.856781314119507E-4	1.1469753743240157E-5	0.11509324393654335	2.8674384358100394E-4
62-63	6.881852245944095E-5	7.914130082835708E-4	1.1469753743240157E-5	0.12091987883810937	2.8674384358100394E-4
64-65	6.881852245944095E-5	8.71701284486252E-4	1.1469753743240157E-5	0.12708487147510095	2.8674384358100394E-4
66-67	6.881852245944095E-5	8.71701284486252E-4	1.1469753743240157E-5	0.13315237120527498	2.8674384358100394E-4
68-69	6.881852245944095E-5	8.889059151011122E-4	1.1469753743240157E-5	0.1397245401001516	2.8674384358100394E-4
70-71	6.881852245944095E-5	9.061105457159724E-4	1.1469753743240157E-5	0.14629097411815659	2.8674384358100394E-4
72-73	6.881852245944095E-5	9.175802994592126E-4	1.1469753743240157E-5	0.15112547532093232	3.4409261229720474E-4
74-75	6.881852245944095E-5	9.347849300740728E-4	1.1469753743240157E-5	0.15501945671676234	3.4409261229720474E-4
76-77	6.881852245944095E-5	9.806639450470334E-4	1.1469753743240157E-5	0.15844317820911952	3.4409261229720474E-4
78-79	6.881852245944095E-5	0.0010093383294051338	1.1469753743240157E-5	0.16154574659666598	3.4409261229720474E-4
80-81	6.881852245944095E-5	0.001020808083148374	1.1469753743240157E-5	0.16290491241523997	3.67032119783685E-4
82-83	7.455339933106102E-5	0.0010265429600199941	1.1469753743240157E-5	0.163432521087429	4.129111347566457E-4
84-85	8.02882762026811E-5	0.0010380127137632343	1.1469753743240157E-5	0.1638339624684424	4.2438088849988583E-4
86-87	8.02882762026811E-5	0.0010666870981213346	1.1469753743240157E-5	0.16393719025213155	4.2438088849988583E-4
88-89	8.02882762026811E-5	0.0011355056205807755	1.1469753743240157E-5	0.1639715995133613	4.473203959863661E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10815	0.0	52.11488	1
GTATCAA	20130	0.0	37.044678	1
TCAACGC	25070	0.0	29.220459	4
ATCAACG	25255	0.0	29.041277	3
CAACGCA	25810	0.0	28.382677	5
TATCAAC	26040	0.0	28.230526	2
AACGCAG	26480	0.0	27.696518	6
ACGCAGA	30670	0.0	23.940353	7
CGCAGAG	31300	0.0	23.458488	8
GCAGAGT	36320	0.0	20.017956	9
GTACATG	25095	0.0	19.67531	1
TACATGG	25350	0.0	19.210121	2
GAGTACT	21585	0.0	19.027756	12-13
TGGTATC	5290	0.0	18.57131	2
ACATGGG	25395	0.0	18.525974	3
AGAGTAC	32965	0.0	18.38696	10-11
GTGGTAT	5720	0.0	18.285683	1
CAGAGTA	35830	0.0	17.862118	10-11
GTACTTT	24075	0.0	16.972374	14-15
AGTACTT	23735	0.0	16.751135	12-13
>>END_MODULE