FastQCFastQC Report
Fri 27 May 2016
SRR2139641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8898173
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112850.044.6873251
GTATCAA190750.033.4890481
TCAACGC233150.026.9766544
ATCAACG234450.026.827073
CAACGCA239800.026.2109725
TATCAAC249150.025.419312
AACGCAG247600.025.4024316
ACGCAGA284550.022.0301157
CGCAGAG291850.021.5079698
GTACATG226600.018.6636141
AGAGTAC295750.018.46617910-11
GCAGAGT339300.018.3510539
TACATGG226550.018.3141082
GAGTACT195450.017.91295212-13
ACATGGG228700.017.8612673
CAGAGTA331000.016.8753310-11
AGTACTT215950.016.47602512-13
GTACTTT218900.016.01312314-15
TATACTG48850.015.7058515
CATGGGG161600.015.2865994
Produced by FastQC (version 0.11.5)