##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139641_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8898173
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.459440606515514	34.0	31.0	34.0	31.0	34.0
2	32.590320844514935	34.0	31.0	34.0	31.0	34.0
3	32.65000613047195	34.0	31.0	34.0	31.0	34.0
4	36.183338422393	37.0	35.0	37.0	35.0	37.0
5	36.10050108039032	37.0	35.0	37.0	35.0	37.0
6	36.13652105887355	37.0	36.0	37.0	35.0	37.0
7	36.08891881513205	37.0	36.0	37.0	35.0	37.0
8	36.061131088370615	37.0	35.0	37.0	35.0	37.0
9	37.77452596167775	39.0	38.0	39.0	35.0	39.0
10-11	37.700613148339556	39.0	37.5	39.0	35.0	39.0
12-13	37.65316734120589	39.0	37.0	39.0	35.0	39.0
14-15	38.99251565461809	40.0	38.0	41.0	35.5	41.0
16-17	38.962103625092475	40.0	38.0	41.0	35.0	41.0
18-19	38.95318814322896	40.0	38.0	41.0	35.0	41.0
20-21	38.91823711693665	40.0	38.0	41.0	35.0	41.0
22-23	38.81621987487344	40.0	38.0	41.0	35.0	41.0
24-25	38.690188438733	40.0	38.0	41.0	34.0	41.0
26-27	38.625042014939424	40.0	38.0	41.0	34.5	41.0
28-29	38.56929219051713	40.0	38.0	41.0	34.0	41.0
30-31	38.50491847113031	40.0	38.0	41.0	34.0	41.0
32-33	38.40462512181939	40.0	38.0	41.0	34.0	41.0
34-35	38.297488835945096	40.0	38.0	41.0	34.0	41.0
36-37	38.44120285732837	40.0	38.0	41.0	34.0	41.0
38-39	38.51124465393282	40.0	38.0	41.0	34.0	41.0
40-41	38.44495755048273	40.0	38.0	41.0	34.0	41.0
42-43	38.40698996738695	40.0	38.0	41.0	34.0	41.0
44-45	38.37348779453484	40.0	38.0	41.0	34.0	41.0
46-47	38.28225754189761	40.0	38.0	41.0	33.0	41.0
48-49	38.203765314220945	40.0	38.0	41.0	33.0	41.0
50-51	38.13621951236678	40.0	37.5	41.0	33.0	41.0
52-53	38.057102470867854	40.0	37.0	41.0	33.0	41.0
54-55	37.928219166910985	40.0	37.0	41.0	33.0	41.0
56-57	37.76718173787653	40.0	36.5	41.0	33.0	41.0
58-59	37.63223334998993	39.0	36.0	41.0	33.0	41.0
60-61	37.478979099114795	39.0	36.0	41.0	32.5	41.0
62-63	37.24922303944666	39.0	35.5	40.5	32.0	41.0
64-65	36.91180969028755	38.5	35.0	40.0	31.5	41.0
66-67	36.602119435734394	38.0	35.0	40.0	31.0	41.0
68-69	36.31212257045513	37.0	35.0	40.0	31.0	41.0
70-71	35.99168117531339	37.0	35.0	39.5	31.0	41.0
72-73	35.672188340506786	36.0	34.0	39.0	31.0	41.0
74-75	35.34104233007676	36.0	34.0	39.0	30.0	40.0
76-77	34.35586339942539	35.0	33.5	37.0	29.0	39.0
78-79	34.67581964800485	35.0	34.0	37.0	30.0	39.0
80-81	34.52311265674003	35.0	34.0	37.0	30.0	39.0
82-83	34.2896561853767	35.0	34.0	36.0	30.0	38.5
84-85	34.035868355786604	35.0	34.0	36.0	30.0	37.0
86-87	33.81731367557574	35.0	34.0	35.5	30.0	37.0
88-89	33.62054017199236	35.0	33.0	35.0	29.5	36.5
90-91	33.46094686920681	35.0	33.0	35.0	29.0	36.0
92-93	33.33729764895349	35.0	33.0	35.0	29.0	36.0
94-95	33.2829563321868	35.0	33.0	35.0	29.0	36.0
96-97	33.305824403628336	35.0	33.0	35.0	29.5	35.0
98-99	33.35563957811928	35.0	33.0	35.0	30.0	35.0
100-101	31.889294078492433	33.5	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	47.0
21	280.0
22	1332.0
23	4458.0
24	11274.0
25	23524.0
26	41303.0
27	63800.0
28	91367.0
29	121402.0
30	155923.0
31	192908.0
32	242893.0
33	311476.0
34	423162.0
35	602740.0
36	987398.0
37	1874418.0
38	2450392.0
39	1232126.0
40	65944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.01898738089268	21.70048840363072	15.14931211159864	24.131212103877953
2	13.905220551636507	22.374313017274442	40.797927372611156	22.922539058477895
3	16.868181816649326	26.931719578839385	31.666983772961032	24.53311483155025
4	11.925043489264594	19.093076747327796	39.68161778828081	29.30026197512681
5	12.682333777956442	38.26841757291075	36.198071222036255	12.851177427096552
6	28.917048477254824	39.34337981515981	19.00019251142903	12.739379196156333
7	25.65159162448291	33.2076933096266	23.82201379991151	17.318701265978984
8	24.09260867371313	36.90652002382961	21.531824566683518	17.469046735773734
9	25.045838061363835	16.856730027613533	21.67996733711516	36.41746457390747
10-11	23.388523949579973	27.15800093300492	29.801291979180792	19.65218313823431
12-13	24.45499596792796	24.74094688706675	29.67403854145027	21.130018603555023
14-15	21.641998113994436	26.304164080461028	27.202904956535452	24.850932849009084
16-17	21.237966490424494	29.453130434753295	27.76377240586354	21.545130668958674
18-19	21.226166315264944	28.662400697311686	29.452838239939815	20.658594747483555
20-21	21.99624527674324	27.748252262243522	29.352638126057172	20.902864334956064
22-23	21.7302454153637	27.61298811127079	29.20573883460466	21.45102763876085
24-25	21.5777004715947	27.767153236909724	29.199925976743835	21.45522031475174
26-27	21.534326750071166	27.919171052417695	29.142278546172818	21.40422365133832
28-29	21.53462947150025	27.942488161859664	29.158671305476947	21.36421106116314
30-31	21.733156601851746	27.852797846213655	29.045594043129423	21.368451508805176
32-33	21.570880524891813	27.994764407694728	28.940267913339866	21.4940871540736
34-35	21.715301140844907	27.95943652454366	28.953110430703667	21.372151903907767
36-37	21.685829223951746	27.870823508449988	28.94351393650541	21.499833331092855
38-39	21.53624593858873	28.055778434579775	28.97736249791039	21.4306131289211
40-41	21.828094171067498	27.94049664475009	28.789740616114145	21.441668568068263
42-43	21.675849873187985	28.05714684598739	28.5488226260558	21.71818065476883
44-45	21.776505559888776	28.118411781800358	28.407244493766765	21.697838164544102
46-47	21.876414405946978	28.083818249195673	28.161500948225164	21.878266396632178
48-49	21.778543929621197	28.179838550293564	28.182069827582843	21.8595476925024
50-51	21.81020973388586	28.261078461700826	28.072018061734905	21.856693742678406
52-53	21.98618794552133	28.191846522195917	27.90211756577839	21.919847966504356
54-55	21.971080542197946	28.081993985872415	27.959982939274262	21.986942532655373
56-57	21.93395003348387	28.178042166670707	27.909897608374884	21.978110191470535
58-59	21.911446660452818	28.209723431313975	27.91484282609453	21.963987082138676
60-61	21.986066076426034	28.156482045772925	27.948817626806505	21.90863425099453
62-63	21.94275681712516	28.234539323696712	27.89733623780939	21.925367621368743
64-65	21.899308123178972	28.14953298120459	27.90138031121127	22.049778584405168
66-67	21.93763347799796	28.274357573180968	27.706055478424208	22.081953470396865
68-69	21.930593768122467	28.246791247726005	27.745260685469457	22.077354298682074
70-71	21.950294404992157	28.02005608888046	27.81729258877569	22.2123569173517
72-73	21.99779445218854	28.072139617913344	27.73619040176661	22.19387552813151
74-75	21.897030333724516	28.174529417931094	27.708340346474664	22.220099901869727
76-77	22.004250010632312	28.130854925944647	27.663255238399316	22.201639825023722
78-79	21.975490953221925	28.11076700643755	27.596085031432654	22.31765700890788
80-81	22.01604393713666	28.139116334661185	27.577179347001213	22.26766038120094
82-83	22.054304124495484	28.12437320902703	27.65813084605372	22.163191820423762
84-85	22.13180926396994	28.13080864779026	27.606946333628642	22.130435754611156
86-87	22.031686638388514	28.1303016903935	27.69179778085396	22.146213890364024
88-89	22.037392772163678	28.12718232226897	27.6518475429475	22.183577362619854
90-91	22.058116913534725	28.175363659249157	27.67613510682413	22.090384320391987
92-93	22.151971041004558	28.089958411324883	27.669831167018653	22.088239380651903
94-95	21.94971010321148	28.14614720367424	27.7396907134972	22.164451979617077
96-97	21.9332017972715	28.17712294288981	27.806240073360133	22.08343518647856
98-99	22.117507218279314	28.72897128686661	27.856721357947084	21.29680013690699
100-101	22.345605394686846	31.601118842176028	25.07532147675476	20.97795428638237
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3442.0
1	2838.0
2	2971.0
3	3843.5
4	4753.0
5	6777.5
6	8517.5
7	10074.5
8	11312.0
9	11383.5
10	10668.5
11	10087.5
12	11329.5
13	15261.5
14	24291.0
15	36367.0
16	45868.0
17	50962.5
18	52082.0
19	50256.0
20	46933.0
21	44056.0
22	43545.0
23	47710.5
24	56889.0
25	71288.5
26	90474.0
27	110963.0
28	133051.5
29	156118.5
30	176023.5
31	195312.0
32	216070.0
33	237171.0
34	257935.5
35	277693.5
36	300236.5
37	318414.0
38	330644.5
39	342157.0
40	351942.0
41	360587.0
42	364942.5
43	368145.0
44	371516.0
45	373987.5
46	374040.5
47	369766.5
48	364149.5
49	356442.0
50	345991.5
51	331944.0
52	314380.5
53	295188.0
54	274039.0
55	252025.0
56	229625.5
57	205994.5
58	181801.0
59	157145.5
60	131451.5
61	106399.0
62	84167.0
63	64787.0
64	47793.0
65	35167.0
66	26349.0
67	19783.5
68	15044.0
69	11220.0
70	8146.5
71	5782.5
72	4041.0
73	2744.5
74	1817.0
75	1166.5
76	738.0
77	482.0
78	342.5
79	237.5
80	161.0
81	106.0
82	70.0
83	44.0
84	27.5
85	24.5
86	23.5
87	20.0
88	15.0
89	11.0
90	8.0
91	4.5
92	3.5
93	3.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.022757480664851087
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	4.7200700638209666E-4
12-13	0.0016014523430821137
14-15	2.2476524113433175E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008189782153481014
26-27	0.027446238043511144
28-29	0.3270105184922752
30-31	0.3126629068911263
32-33	0.3148870471984355
34-35	0.16948173791346768
36-37	0.030675665849009555
38-39	0.02904545671287042
40-41	0.011334974288249897
42-43	0.008841156981722071
44-45	0.003252990916698125
46-47	0.0020418967535088127
48-49	0.0018233761500840104
50-51	0.0018648961455684648
52-53	2.992680209582892E-4
54-55	5.543993591143408E-5
56-57	3.0468053344462673E-4
58-59	6.027545506241931E-4
60-61	0.00321512475857235
62-63	0.0027476095796656907
64-65	0.0028535125088994325
66-67	0.0067722093132207666
68-69	0.010412299938049131
70-71	0.0144609188477213
72-73	0.013573574534103827
74-75	0.010536598742052134
76-77	0.005133772585390768
78-79	0.002345953556721961
80-81	0.002134648960180472
82-83	3.53272743933568E-4
84-85	2.191769772996213E-5
86-87	0.0
88-89	6.613623402847526E-4
90-91	0.0025501748816783754
92-93	0.015429596622044305
94-95	4.428300127408521E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	170.0
20-21	717.0
22-23	1438.0
24-25	2929.0
26-27	5469.0
28-29	9017.0
30-31	19557.0
32-33	26813.0
34-35	39022.0
36-37	53249.0
38-39	60748.0
40-41	65060.0
42-43	76696.0
44-45	90791.0
46-47	89619.0
48-49	80447.0
50-51	72881.0
52-53	68347.0
54-55	74566.0
56-57	77494.0
58-59	78636.0
60-61	78614.0
62-63	77929.0
64-65	84659.0
66-67	87470.0
68-69	88600.0
70-71	87987.0
72-73	84428.0
74-75	86199.0
76-77	87126.0
78-79	91443.0
80-81	92374.0
82-83	90904.0
84-85	92302.0
86-87	96278.0
88-89	102834.0
90-91	108828.0
92-93	113032.0
94-95	128738.0
96-97	193418.0
98-99	760107.0
100-101	5271237.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.34148312553566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.57580332577413	78.20449194434164
2	6.649351415179517	11.482297260189576
3	1.5745107417790314	4.0783677792686674
4	0.5510262203340489	1.9030568441879991
5	0.2567998854017919	1.1086241486029151
6	0.1344945696789485	0.6967476371046667
7	0.07036140057094473	0.4252575376059695
8	0.048794270732443426	0.33703757624544667
9	0.03442307433508606	0.2674925362648704
>10	0.1023565653332265	1.3348898074421494
>50	0.0017564045476360156	0.09590568998869763
>100	2.81700747948254E-4	0.044470084618939684
>500	4.042558521590653E-5	0.02136115413846651
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.495304822686635E-5	0.0	0.0	0.0
3	0.0	4.495304822686635E-5	0.0	0.0	0.0
4	0.0	8.99060964537327E-5	0.0	0.0	1.1238262056716587E-5
5	0.0	8.99060964537327E-5	0.0	0.0	1.1238262056716587E-5
6	0.0	8.99060964537327E-5	0.0	0.0	3.371478617014976E-5
7	0.0	8.99060964537327E-5	0.0	0.0	3.371478617014976E-5
8	0.0	1.0114435851044928E-4	0.0	0.0	3.371478617014976E-5
9	0.0	1.798121929074654E-4	0.0	1.1238262056716587E-5	4.495304822686635E-5
10-11	0.0	1.798121929074654E-4	0.0	2.2476524113433175E-5	4.495304822686635E-5
12-13	0.0	1.798121929074654E-4	0.0	2.2476524113433175E-5	4.495304822686635E-5
14-15	0.0	1.9666958599254026E-4	0.0	3.933391719850805E-5	6.181044131194123E-5
16-17	0.0	2.0228871702089856E-4	0.0	7.304870336865781E-5	7.86678343970161E-5
18-19	0.0	2.1914611010597344E-4	0.0	8.42869654253744E-5	8.99060964537327E-5
20-21	0.0	2.6971828936119804E-4	0.0	8.99060964537327E-5	1.0114435851044928E-4
22-23	0.0	3.596243858149308E-4	0.0	1.4047827570895733E-4	1.1238262056716586E-4
24-25	0.0	4.3829222021194683E-4	0.0	1.741930618791071E-4	1.2362088262388244E-4
26-27	0.0	4.832452684388132E-4	0.0	2.697182893611981E-4	1.2924001365224074E-4
28-29	0.0	4.888643994671715E-4	0.0	9.889670609910597E-4	1.3485914468059905E-4
30-31	0.0	5.506748407791127E-4	0.0	0.0030118542312000452	1.3485914468059905E-4
32-33	0.0	5.619131028358293E-4	0.0	0.007063247702646375	1.3485914468059905E-4
34-35	0.0	6.405809372328454E-4	0.0	0.01320495791664199	1.3485914468059905E-4
36-37	0.0	7.192487716298616E-4	0.0	0.022375379754922722	1.3485914468059905E-4
38-39	0.0	7.642018198567279E-4	0.0	0.03692330998734235	1.4609740673731563E-4
40-41	0.0	8.260122611686691E-4	0.0	0.06537858951494874	1.4609740673731563E-4
42-43	0.0	8.428696542537441E-4	0.0	0.08007261715410569	1.4609740673731563E-4
44-45	0.0	9.271566196791183E-4	0.0	0.10113873937942092	1.4609740673731563E-4
46-47	0.0	0.0010002053230477761	0.0	0.125166143656681	1.4609740673731563E-4
48-49	0.0	0.0010058244540761345	0.0	0.14572092495841563	1.4609740673731563E-4
50-51	0.0	0.0011406835987567334	0.0	0.16579808012273978	1.4609740673731563E-4
52-53	0.0	0.0011463027297850918	0.0	0.1937026848095671	1.573356687940322E-4
54-55	0.0	0.0011575409918418084	0.0	0.2499333290103485	1.741930618791071E-4
56-57	0.0	0.0011800175159552416	0.0	0.3078103786024389	1.854313239358237E-4
58-59	0.0	0.0011800175159552416	0.0	0.3538872530349769	1.9105045496418198E-4
60-61	0.0	0.0011968749090403167	0.0	0.37989259143421916	2.0228871702089856E-4
62-63	0.0	0.0012137323021253915	0.0	0.40190834680332693	2.1914611010597342E-4
64-65	0.0	0.0014047827570895734	0.0	0.41305670276358974	2.2476524113433172E-4
66-67	0.0	0.0014272592812030066	0.0	0.4204458600658809	2.3038437216269002E-4
68-69	0.0	0.001494688853543306	0.0	0.4279417808577109	2.3600350319104833E-4
70-71	0.0	0.0015396419017701724	0.0	0.43554446513907963	2.3600350319104833E-4
72-73	0.0	0.0015621184258836057	0.0	0.4410568326778992	2.8095655141791465E-4
74-75	0.0	0.0015902140810253968	0.0	0.445445374011047	2.8657568244627295E-4
76-77	0.0	0.001663262784394055	0.0	0.44921581093107543	3.0343307553134787E-4
78-79	0.0	0.0016969775705642045	0.0	0.4526828147755725	3.146713375880644E-4
80-81	0.0	0.0016969775705642045	0.0	0.45406512100854857	3.146713375880644E-4
82-83	0.0	0.0017082158326209211	0.0	0.4546101767182994	3.427669927298559E-4
84-85	0.0	0.0017194540946776377	0.0	0.45501475415234116	3.483861237582142E-4
86-87	0.0	0.0017475497498194294	0.0	0.4551439941659934	3.483861237582142E-4
88-89	0.0	0.0018430749773015202	0.0	0.4551833280831919	3.652435168432891E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11285	0.0	44.687325	1
GTATCAA	19075	0.0	33.489048	1
TCAACGC	23315	0.0	26.976654	4
ATCAACG	23445	0.0	26.82707	3
CAACGCA	23980	0.0	26.210972	5
TATCAAC	24915	0.0	25.41931	2
AACGCAG	24760	0.0	25.402431	6
ACGCAGA	28455	0.0	22.030115	7
CGCAGAG	29185	0.0	21.507969	8
GTACATG	22660	0.0	18.663614	1
AGAGTAC	29575	0.0	18.466179	10-11
GCAGAGT	33930	0.0	18.351053	9
TACATGG	22655	0.0	18.314108	2
GAGTACT	19545	0.0	17.912952	12-13
ACATGGG	22870	0.0	17.861267	3
CAGAGTA	33100	0.0	16.87533	10-11
AGTACTT	21595	0.0	16.476025	12-13
GTACTTT	21890	0.0	16.013123	14-15
TATACTG	4885	0.0	15.705851	5
CATGGGG	16160	0.0	15.286599	4
>>END_MODULE