FastQCFastQC Report
Fri 27 May 2016
SRR2139640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8401853
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111250.052.7783051
GTATCAA200150.037.3579751
ATCAACG257200.028.5996743
TCAACGC258400.028.4668584
TATCAAC267300.027.7653752
CAACGCA264950.027.7473285
AACGCAG272450.027.030676
ACGCAGA310500.023.6639217
CGCAGAG318400.023.0901938
TGGTATC52100.020.654542
GTGGTAT54950.020.5158231
GCAGAGT362400.020.0629089
GTACATG258300.019.6732141
TACATGG256850.019.3686392
GAGTACT216250.018.67652112-13
AGAGTAC335400.018.62296110-11
ACATGGG260200.018.474723
CAGAGTA355550.018.3960910-11
AGTACTT235850.017.14253612-13
GTACTTT237600.017.14215914-15
Produced by FastQC (version 0.11.5)